• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.295-300
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• 2004

The identification of the leptin gene in 1994 and it's adipocytes specific protein leptin hal provided the first physiological links to the regulatory system controlling body weight and fat deposits. The meat tastes is mainly determined by quantifY and quality of triglyceride stored in adipose tissue. This study was conducted to analyze genetic cbaracteristics of Hanwoo leptin gene and also to investigate the association of DNA marlcer with some economic meat traits for Hanwoo. The leptin hormone gene polymorphisms were identified by digestion with Kpn2 I and Msp I. Slaughter weight(SWI), slaughter peroentage(SP), longissimus muscle area(LMA), beef marbling score(MS) and back fat thickness(BF) were compared among three genotypes by P(R..RFlJ> and showed significant differences among genotypes. PCR-RFLP(Kpn2 I) were detected significant for SP, MS and BF. The allele was essociated with fatter carcasses and C allele with leaner carcasses.

• Korean Chemical Engineering Research
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• v.43 no.3
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• pp.425-431
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• 2005

New type of supercapacitor using high surface area activated carbons mixed with high conductivity polypyrrole (Ppy) has been prepared in order to achieve low impedance and high energy density. Mixed carbons of BP-20 and MSP-20 were used as the active electrode material, and polypyrrole doped with 2-naphthalenesulfonic acid (2-NSA) and carbon black (Super P) as conducting agents were added to activated carbons in order to enhance good electric conductivity. Electrodes prepared with the activated electrode materials and the conducting agents were added to a solution of organic binder [P(VdF-co-HFP) / NMP]. The ratio of optimum electrode composition was 78 : 17 : 5 wt.％ of (MSP20 : BP-20=1 : 1), (Super P : Ppy=10 : 7) and P(VdF-co-HFP) respectively. The performance of unit cell with addition of 7 wt％ Ppy have shown specific capacitance of 28.02 F/g, DC-ESR of $1.34{\Omega}$, AC-ESR of $0.36{\Omega}$, specific energy of 19.87 Wh/kg and specific power of 9.77 kW/kg. With addition of Ppy, quick charge-discharge of unit cell was possible because of low ESR, low charge transfer resistance and quick reaction rate. And good stability up to 500 chargedischarge cycles were retained about 80％ of their original capacity. It was concluded that the specific capacitance originated highly from compound phenomena of the pseudocapacitance by oxidation-reduction of polypyrrole and the nonfaradaic capacitance by adsorption-desorption of activated carbons.

• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.106-112
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• 2001

In view of the effect of factor Ⅶ as a risk factor for essential hypertension, we investigated the length (I/D) polymorphism at position 323 promoter region and exon 8-Msp I RFLP of the human factor Ⅶ gene in the Korean patients with essential hypertension and normal controls. There were no significant differences in the allele, genotype and haplotype frequencies of these polymorphisms between normotensive and essential hypertensive subjects. The significant linkage disequilibrium was however, detected between two polymorphic sites. The Msp I RFLP and I/D polymorphism were also significantly associated with plasma triglyceride (TG) levels. Therefore, our results suggest that the significant association between two genetic variations in the human factor Ⅶ gene and plasma TG level may reflect the potential role of human factor Ⅶ gene as one of the genetic components for cardiovascular risk.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.369-374
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• 2008

The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

• Journal of Power Electronics
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• v.7 no.2
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• pp.132-139
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• 2007

Electrical motors are an integral part of industrial plants with no less than 5 billion motors built world wide every year. The demand for low-cost brushless DC (BLDC) motors has increased in industrial applications. This paper presents a BLDC motor control algorithm for low-cost motor drive applications using general purpose microcontrollers which have only one on-chip timer. This paper describes how to realize pulse width modulation (PWM) signals with general input/output (I/O) ports to control a three-phase permanent magnet brushless DC motor using the timer interrupt on MSP430F1232.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.07a
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• pp.595-596
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• 2005

This research which it sees adds $LiMn_2O_4$ in the activated carbon electrode the test against the effect which it follows is. Test cells, which were $LiMn_2O_4$fabricated with active mass composite consisted of (100-X)% of MSP-20 and (X)% of $LiMn_2O_4$ (X=20,40,60,80,100), exhibits the better specific capacitance than those of the cells fabricated with single active mass that is MSP-20. The enhanced properties of composite active mass could be caused by capability of $LiMn_2O_4$ powders. But the resistance was increase by proportionate in $LiMn_2O_4$ addition and when mixture ratio of the activated carbon and the $LiMn_2O_4$ being similar, to be low rather to the after where had become the maximum it came.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2209-2213
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• 2015

DNA methylation of tumor suppressor gene promoters is the most frequent phenomenon leading to inactivation of function, consequently driving malignant cell transformation. Cyclin D2 is implicated in tumor suppression. In our study, we carried out the MSP assay to evaluation the methylation status at CpG islands in the cyclin D2 promoter in breast cancer cases from the Vietnamese population. The results showed that the frequency of methylation reached 62.1% (59 of 95 breast cancer tumors), but was low in non-cancer specimens at 10% (2 of 20 non-cancer specimens). Additionally, with an RR (relative risk) and OR (odd ratios) of 6.21 and 14.8, DNA hypermethylation of cyclin D2 increased the possibility of malignant transformation. Our results confirmed the cyclin D2 hypermethylation could be used as the potential biomarker which could be applied in prognosis and early diagnosis of Vietnamese breast cancer patients.

• Journal of Life Science
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• v.22 no.2
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• pp.245-250
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• 2012

The 16S rDNA - RFLP types for six Vibrio species (V. fluvialis, V. proteolyticus, V. vulnificus, V. mimicus) including two core group members, V. alginolyticus and V. parahaemolyticu s, and Grimontia (Vibrio) hollisae were determined using PCR-RFLP analysis. Six tetrameric restriction enzymes (Alu I, Cfo I, Dde I, Hae III, Msp I, and Rsa I) were selected for RFLP analysis. V. alginolyticus, V. parahaemolyticus, and V. proteolyticus showed the same RFLP pattern following digestion with four of the six used restriction enzymes: CfoI, DdeI, MspI, and RsaI. Various restriction enzyme combinations generated digests recognizable as distinct RFLP types for each of the assayed Vibrio species. In particular, AluI single digestion produced species specific band patterns that enabled the differentiation between these Vibrio species. Dendrogram based on restriction patterns showed that two Vibrio core group members, V. alginolyticus and V. parahaemolyticus were closely related having a similarity over 90%. Although the observed RFLP pattern for Grimontia hollisae shared several common bands with other Vibrio spp., G. hollisae results were still clearly distinct from Vibrio spp. RFLP types for all restriction enzymes tested. If restriction enzymes are aptly selected, PCR-RFLP analysis is still a rapid and effective tool for differentiating Vibrio species.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1995.11a
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• pp.167-170
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• 1995

LiCoO$_2$is a electrode material of Li ion Cell which is expected as the cell with a very high electric charge density. The recent study is mainly to focused on a high power secondary cell. If very thin Li ion Cell can be made in the scale of IC substrate it can be a electric souse in IC chip , micro machine or very thin electrical display etc. LiCoO$_2$thin film can be made by CVD, Laser ablation, E-Beam, ton Beam process, sputtering etc. But to make the material with a high quality for a cell is difficult as the electrode in cell have the fitable ratio in components and a lattice structure of bulk etc. In this study, LiCoO$_2$is made by R.F magnetron sputtering with the variance of substrate temperature and oxygen partial pressure etc. In the substrate temperature of 600$^{\circ}C$ and the oxygen rate of 10%, we can acquire the good thin film LiCoO$_2$compared wish a bulk material.

• Journal of Astronomy and Space Sciences
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• v.30 no.3
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• pp.159-162
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• 2013

We report the detection of a quasi-sinusoidally modulated optical flux with a period of 4.6343 hour in the optical and infrared band of the Fermi source 2FGL J2339.7-0531. Comparing the multi-wavelength observations, we suggest that 2FGL J2339.7- 0531 is a ${\gamma}$-ray emitting millisecond pulsar (MSP) in a binary system with an optically visible late-type companion accreted by the pulsar, where the MSP is responsible for the ${\gamma}$-ray emission while the optical and infrared emission originate from the heated side of the companion. Based on the optical properties, the companion star is believed to be heated by the pulsar and reaches peak magnitude when the heated side faces the observer. We conclude that 2FGL J2339.7-0531 is a member of a subclass of ${\gamma}$-ray emitting pulsars -the 'black widows'- recently revealed to be evaporating their companions in the late-stage of recycling as a prominent group of these newly revealed Fermi sources.