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http://dx.doi.org/10.7314/APJCP.2015.16.6.2209

Loss of Expression of Cyclin D2 by Aberrant DNA Methylation: a Potential Biomarker in Vietnamese Breast Cancer Patients  

Truong, Phuong Kim (Molecular, Biotechnology, Ho Chi Minh City Open University)
Lao, Thuan Duc (Molecular, Biotechnology, Ho Chi Minh City Open University)
Doan, Thao Phuong Thi (Department of Pathological Anatomy, Faculty of Medicine, Ho Chi Minh City Medicine and Pharmacy University)
Huyen Le, Thuy Ai (Molecular, Biotechnology, Ho Chi Minh City Open University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.6, 2015 , pp. 2209-2213 More about this Journal
Abstract
DNA methylation of tumor suppressor gene promoters is the most frequent phenomenon leading to inactivation of function, consequently driving malignant cell transformation. Cyclin D2 is implicated in tumor suppression. In our study, we carried out the MSP assay to evaluation the methylation status at CpG islands in the cyclin D2 promoter in breast cancer cases from the Vietnamese population. The results showed that the frequency of methylation reached 62.1% (59 of 95 breast cancer tumors), but was low in non-cancer specimens at 10% (2 of 20 non-cancer specimens). Additionally, with an RR (relative risk) and OR (odd ratios) of 6.21 and 14.8, DNA hypermethylation of cyclin D2 increased the possibility of malignant transformation. Our results confirmed the cyclin D2 hypermethylation could be used as the potential biomarker which could be applied in prognosis and early diagnosis of Vietnamese breast cancer patients.
Keywords
Cyclin D2; methylation; MSP; breast cancer; Vietnamese population;
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Times Cited By KSCI : 3  (Citation Analysis)
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