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Effect of Carbon Sources and Culture Temperature on Pectate Lyase Production in Phytopathogenic Bacteria (탄소원과 배양온도가 식물 병원세균의 Pectate lyase 생산에 미치는 영향)

  • 한광섭;최재을
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.125-129
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    • 1998
  • Phytopathogenic bacteria causing soft-rot many vegetables; extracellular enzymes produced by them, pectate lyase(Pel) is important pathogenicity facotrs which cause tissue maceration and cell death. Ten of seventeen plant pathogenic bacteria showed weak Pel activity, four of them showed low Pel activity and Erwinia acrotovora subsp. carotovora, E. chrysanthemi, Pseudomonas marginalis and Xanthomonas campestris pv. campestris showed high Pel activity in the polygalacturonate yeast extract agar (PAY) plate. High Pel activity of the four bacteria species produced the highest Pel activity when pectin or polygalacturonic acid (PGA) was added to minimal salts (MS) medium. Pel activity of the four bacterial species was the highest at 2$0^{\circ}C$ among different temperature conditions. The rate and amount of maceration of potato tuber tissue were highest at 2$0^{\circ}C$ in E. carotovora subsp. carotovora, E. chrysanthemi and P. marginalis, while those were the highest at $25^{\circ}C$ in X. campestris pv. campetris.

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Antioxidant Activity of Ergosterol Peroxide (5,8-Epidioxy-$5\alpha,8\alpha$-ergosta-6,22E-dien-3$\beta$-ol) in Armillariella mellea

  • 김상욱;박상신;민태진;유국현
    • Bulletin of the Korean Chemical Society
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    • v.20 no.7
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    • pp.819-823
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    • 1999
  • Antioxidant activities of various mushroom fruiting bodies were investigated in vitro. Among the mushroom extracts examined, Armillariella mellea, Daedalea dickinsi, Fomitella fraxinea and Pleurotus cornusopiae markedly exhibited inhibition on lipid peroxidaton of rat liver microsomes. Ergosterol peroxide (5,8-epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol), antioxidant from A. mellea, was isolated by solvent extraction, silica gel column chromatography and recrystallization. The structure of the compound was determined by NMR, GC/MS and X-ray crystallography. Ergosterol peroxide showed potent inhibition on lipid peroxidation and exhibited higher antioxidant activity than well-known antioxidants, α-tocopherol and thiourea.

Fabrication and Characterization of AlN films Containing Various Amounts of Co Content

  • Bae, Chang-Hwan;Han, Seung-Oh;Han, Cahng-Suk
    • Korean Journal of Metals and Materials
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    • v.48 no.3
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    • pp.268-275
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    • 2010
  • A new approach is described for preparing AlN thin films containing various amounts of Co content by using a two-facing targets type sputtering (TFTS) system. The deposited films were annealed isothermally at different temperatures and their microstructure, magnetic properties and resistivity were investigated. A small saturation magnetization ($4{\pi}Ms=0.52{\sim}0.85kG$) was observed irrespective of Co content in the asdeposited films. It was found that annealing conditions can control physical properties as well as the microstructure of the films. A high saturation magnetization (3.7 kG) and resistivity of $2200{\mu}{\Omega}-cm$ was obtained for AlN films containing 25 at.% Co.

Gemological Comparison between Gwangdong Tektite and Baikdusan Obsidian (텍타이트와 흑요석의 보석학적 특징비교 - 중국 광동 텍타이트와 백두산 흑요석을 중심으로 -)

  • Kim, Won-Sa
    • Journal of the Mineralogical Society of Korea
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    • v.20 no.3
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    • pp.181-191
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    • 2007
  • The gemological charateristics of Gwangdong tektites and Baikdusan obsidians were investigated, using microscopy, density and refractive index measurements, X-ray fluorescence spectrometry, Inductively Coupled Plasma Mass spectrometry, X-ray powder diffraction, and electron microprobe analysis. The Gwangdong tektites and Baikdusan obsidians are both black in colour and slightly trans-lucent with various shades of brown when cut into a few mm thick. Both the materials yield conchoidal fracture on broken surface. The tektites occur as tear-drop shapes, ranging from 4 to 10 cm long, and in spheres, from 3 to 5 cm in diameter. On the surface numerous shallow pits up to 3 mm in diameter are present. Mohs' hardness and specific gravity are 5 to 5.5 and 2.66, respectively. The tektites are singly refractive, with an refractive index of 1.51. Numerous spherical air bubbles are randomly scattered throughout the tektites, and silica-rich glass inclusions are occasionally seen. X-ray powder diffraction analysis verifies that they are non-crystalline. The Baikdusan obsidians show very similar properties to those of the Gwangdong tektites, especially in hardness, amorphous nature and fracture. Nevertheless, the Baikdusan obsidian can readily be distinguished from the Gwangdong tektites by refractive index ($1.49{\sim}1.50$), specific gravity (2.67 to 2.68), and inclusions (absence of bubbles and presence of sanidine and magnetite crystals).

Effect of Water Temperature on the Expression of Stress Related Genes in Atlantic Salmon (Salmo salar) Fry (수온이 대서양 연어(Salmo salar) 치어의 체내 스트레스 관련 유전자 발현에 미치는 영향)

  • Kang, Hee Woong;Kim, Kwang Il;Lim, Hyun Jeong;Kang, Han Seung
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.131-139
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    • 2018
  • The warming of water as a result of climate change affects fish habitat. Variations in water temperature affect fish physiology almost totally. The rise in water temperature due to climate change leads to hypoxia following decreased oxygen solubility and decreased binding capacity of oxygen-carrying hemoglobin. This study was conducted to evaluate the health status of Atlantic salmon (Salmo salar) fry at elevated water temperatures($20^{\circ}C$) compared with optimum water temperature ($15^{\circ}C$). The method facilitated the detection of biomarker genes using NGS RNAseq analysis and evaluation of their expression pattern using RT-qPCR analysis. The biomarker genes included interferon alpha-inducible protein 27-like protein 2A transcript variant X3, protein L-Myc-1b-like, placenta growth factor-like transcript variant X1, fibroblast growth factor receptor-like 1 transcript variant X1, transferrin, intelectin, thioredoxin-like, c-type lectin lectoxin-Thr1-like, ladderlectin-like and calponin-1. The selected biomarker genes were sensitive to changes in water temperature based on NGS RNAseq analysis. The expression patterns of these genes based on RT-qPCR were similar to those of NGS RNAseq analysis.

P123-Templated Co3O4/Al2O3 Mesoporous Mixed Oxides for Epoxidation of Styrene

  • Jung, Mie-Won;Kim, Young-Sil
    • Korean Journal of Materials Research
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    • v.22 no.6
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    • pp.316-320
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    • 2012
  • $Co_3O_4$, $Al_2O_3$ and $Co_3O_4$/$Al_2O_3$ mesoporous powders were prepared by a sol-gel method with starting matierals of aluminum isopropoxide and cobalt (II) nitrate. A P123 template is employed as an active organic additive for improving the specific surface area of the mixed oxide by forming surfactant micelles. A transition metal cobalt oxide supported on alumina with and without P123 was tested to find the most active and selective conditions as a heterogeneous catalyst in the reaction of styrene epoxidation. A bBlock copolymer-P123 template was added to the staring materials to control physical and chemical properties. The properties of $Co_3O_4$/$Al_2O_3$ powder with and without P123 were characterized using an X-ray diffractometer (XRD), a Field-Emission Scanning Electron Microscope (FE-SEM), a Bruner-Emmertt-Teller (BET) surface analyzer, and $^{27}Al$ MAS NMR spectroscopy. Powders with and without P123 were compared in catalytic tests. The catalytic activity and selectivity were monitored by GC/MS, $^1H$, and $^{13}C$-NMR spectroscopy. The performance for the reaction of epoxidation of styrene was observed to be in the following order: [$Co_3O_4$/$Al_2O_3$ with P123-1173 K > $Co_3O_4$/$Al_2O_3$ with P123-973 K > $Co_3O_4$-973 K>$Co_3O_4$/$Al_2O_3$-973 K > $Co_3O_4$/$Al_2O_3$ with P123-1473 K > $Al_2O_3$-973 K]. The existence of ${\gamma}$-alumina and the nature of the surface morphology are related to catalytic activity.

Antibacterial and phytochemical properties of Aphanamixis polystachya essential oil

  • Rahman, Md. Shahedur;Ahad, Abir;Saha, Subbroto Kumar;Hong, Jongki;Kim, Ki-Hyun
    • Analytical Science and Technology
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    • v.30 no.3
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    • pp.113-121
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    • 2017
  • Now a day's rise of new antibiotic resistant bacterial strains is a global threat. Ethnic people of India have been employing Aphanamixis polystachya (Wall.) R. Parker wood extract in healing cancerous wounds. The aim of this study was to evaluate the antimicrobial activity and to identify the medicinally potent chemicals in the essential oil extract of A. polystachya. The antibacterial properties of various organic extracts were evaluated against a range of bacteria (gram-positive and gram-negative bacteria) based on the disc diffusion method and GC-MS based analysis for finding active oil extract components. All extracts of A. polystachya leaves showed potential antibacterial activity, notably ethyl acetate, while petroleum ether extracts revealed highly sensitive activity against all tested bacteria (zones of inhibition ranging from 8.83 to 11.23 mm). In addition, the petroleum ether extract had the lowest MIC value (32 to $256{\mu}g/mL$) against E. coli, S. lutea, X. campestris, and B. subtilis bacteria. The major compounds detected in oil [${\beta}$-elemene (16.04 %), ${\beta}$-eudesmol (12.78 %), ${\beta}$-caryophyllene (19.37 %), ${\beta}$-selinene (11.32 %), elemol (5.76 %), and ${\alpha}$-humulene (5.68 %)] are expected to be responsible for the potent antimicrobial activity. The results of this study offer valuable insights into the potent role of A. polystachya essential oil extract in pharmaceutical and antibiotic research.

Effect of TDZ (Thidiazuron) on Shoot Proliferation of Peace Poplar (Peace 포플러(Populus koreana X P. trichocarpa)의 줄기형성에 미치는 Thidiazuron 효과)

  • Kang, Ho-Duck;Moon, Heung-Kyu;Park, In-Sun;Lee, Min-Soon
    • Journal of Plant Biotechnology
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    • v.31 no.1
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    • pp.49-53
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    • 2004
  • Shoot formation was investigated from in vitro cultivation of exotic hybrid poplar (Populus koreana ${\times}$ P. trichocarpa) with a specific stomatal character occurring both upper and lower surface of leaves. Two different explants (stem and leaf segment) of Peace poplar were cultured on half strength Murashige and Skoog (MS) basal medium supplemented with the various concentrations of thidiazuron as a plant growth regulator. Most adventitious shoots were produced from excised ends of stem or mid-veins of leaf segments. The highest average numbers of shoots were 7.1 and 5.3 with the treatments of 0.02mg/L TDZ in both explants of stem and leaf segment. The highest shooting rates were achieved to 83.3% and 47.6% with the concentrations of 0.01mg/L and 0.02mg/L TDZ by axillary bud and leaf cultures, respectively.

PC용 중소형 S/W개발을 위한 graphic user interface tool kit

  • 신하용;홍태화
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 1992.04b
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    • pp.524-531
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    • 1992
  • S/W의 구성은 기능을 실제로 수행하는 계산처리부와 사용자와 정보를 주고받는 User Interface부로 크게 나누어 볼 수 있다. 대부분의 S/W는 그 기능의 충실도와 아울러 편리한 사용법이 잘 어울려야 제역할을 할 수 있다. 따라서 편리한 User Interface를 만드는 일은 훌륭한 기능을 제공하는 것과 마찬가지로 S/W 개발자들이 지향해야 할 목표중 하나라 하겠다. 그러나 편리한 User Interface를 만드는 일은 전체 시스템개발 기간중 상당한 부분을 차지한다. 특히 CAD/CAM관련 S/W와 같이 사용자와 정보를 Graphic으로 주고받는 경우에는 User Interface의 구성이 더욱 더 어려워지며, 개발기간중 User Interace가 차지하는 비중은 더욱 커지게 된다. GUI는 최근들어 상당히 각광을 받고 있는 분야이며, 상당수의 GUI가 개발되어 있고, 이중 Unix용의 X-Window MS-DOS용의 MS-WINDOWS 3.0이 널리 사용되고 있다. 그러나 이러한 Window System들은 매우 다양한 기능을 제공하고 있음에도 불구하고, GUI자체의 덩치가 매우크고, S/W개발자가 익혀야하는 내용이 너무 복잡하여, 중/소형의 S/W개발에 사용하기에는 적합하지 않다. 본 연구에서는 GUI(Graphic User Interface)부분을 계산처리부와 완전분리하여, 범용성있는 개발용 Tool Kit을 구성함으로써 S/W개발자는 계산처리부만을 만들면되는 방안을 제시하고 있다. GS-GUI는 Menu처리부, Mouse/Keyboard입력처리부, Text/Graphic출력처리부로 나뉘어진다. Menu처리부는 Menu File로 주어지는 Menu Tree를 Pop-Up형태로 보여주며 User가 Menu를 선택할 수 있도록하며 선택된 Menu Item에 대한 Action Code를 계산처리부로 넘겨준다. 입력처리부에서는 Mouse와 Keyboard 어느것으로나 입력이 가능하도록 해준다. 출력처리부에서는 Action Code에 따라 계산처리부에서 계산된 결과를 화면에 보여주기 위한 각종 2D/3D Graphic Routine들이 포함되어있어 계산처리부에서 불러쓰도록 되어있다.

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Artificial Weathering of Biotite and Uranium Sorption Characteristics (흑운모의 인위적 풍화와 우라늄 수착 특성)

  • Lee, Seung-Yeop;Baik, Min-Hoon;Lee, Jae-Kwang
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.7 no.1
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    • pp.33-38
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    • 2009
  • An experiment for uranium sorption onto fresh and weathered biotites was performed. After centrifugation, concentrations of uranium in the supernatants were analyzed using ICP-MS, and biotite samples were investigated using XRD and SEM. With powdered biotites (<3 mm in size), we have conducted uranium sorption experiments about fresh and weathered biotites to obtain uranium sorption amounts in various pH conditions. The uranium sorption was not high at a low pH (e.g., pH 3), but increased with increasing pH. There were lower uranium sorption by the weathered biotites than by the fresh ones, and the difference was much larger at higher pH (e.g., pH 11). The lower sorption values of uranium by the weathered biotites may be caused by a change of mineral surfaces and a chemical behavior of surrounding dissolved elements. It seems that the uranium-mineral interaction has been diminished, especially, in the weathered biotite by a destruction and dissolution of preferential sorption sites on the mineral surfaces and by the colloidal formation from dissolved elements.

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