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http://dx.doi.org/10.5010/JPB.2004.31.1.049

Effect of TDZ (Thidiazuron) on Shoot Proliferation of Peace Poplar  

Kang, Ho-Duck (Laboratory of Forest Biotechnology, Department of Forest Resources, Dongguk University)
Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute)
Park, In-Sun (Department of Environment and Forest Products, Joongbu University)
Lee, Min-Soon (Laboratory of Forest Biotechnology, Department of Forest Resources, Dongguk University)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 49-53 More about this Journal
Abstract
Shoot formation was investigated from in vitro cultivation of exotic hybrid poplar (Populus koreana ${\times}$ P. trichocarpa) with a specific stomatal character occurring both upper and lower surface of leaves. Two different explants (stem and leaf segment) of Peace poplar were cultured on half strength Murashige and Skoog (MS) basal medium supplemented with the various concentrations of thidiazuron as a plant growth regulator. Most adventitious shoots were produced from excised ends of stem or mid-veins of leaf segments. The highest average numbers of shoots were 7.1 and 5.3 with the treatments of 0.02mg/L TDZ in both explants of stem and leaf segment. The highest shooting rates were achieved to 83.3％ and 47.6％ with the concentrations of 0.01mg/L and 0.02mg/L TDZ by axillary bud and leaf cultures, respectively.
Keywords
Adventitious shoot formation; axillary bud; leaf segment; peace poplar; thidiazuron;
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