• 융합보안논문지
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• 제7권4호
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• pp.107-114
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• 2007

보안 평가 체계에서 요구하는 보안 수준은 제시되는 세부지침, 가이드라인 및 우수 구현 사례를 이용하여 달성될 수 있다. 그러나 조직이 2가지 이상의 서로 다른 보안 인증 체계를 요구 받는 경우, 중점 평가 기준, 보안 요구 수준, 평가 항목들이 상이하기 때문에, 보안 아키텍처의 재구축 또는 변경 절차가 요구된다. 따라서 본 연구에서는 ML Analysis를 이용하여 제시되는 다양한 보안 관리 수준을 달성하기 위한 Multi-level 보안 아키텍처(MLSA) 구축 방법론을 제시한다. MLSA는 다양한 보안인증체계의 동시적 달성을 위한 방법론을 제공한다.

• The Plant Pathology Journal
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• 제37권3호
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• pp.243-257
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• 2021

Bacillus pumilus is the causal agent of trunk bulges disease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic relationships of 20 isolates of B. pumilus group from four rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang). Multi rep-PCR results revealed the genetic profiling among the B. pumilus group isolates, while MLSA results showed 98-100% similarity across the 20 isolates of B. pumilus group species. These 20 isolates, formerly established as B. pumilus, were found not to be grouped with B. pumilus. However, being distributed within distinctive groups of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates close to B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. This is the first MLSA and rep-PCR study on B. pumilus group, which provides an in-depth understanding of the diversity of these rubber-pathogenic isolates in Malaysia.

• 한국전자통신학회논문지
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• 제18권5호
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• pp.769-776
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• 2023

본 논문에서는 저 전력 CAM(: Content Addressable Memory)을 위한 MLSA(: Match-line Sense Amplifier)를 설계하였다. 설계한 회로는 MLSA와 사전충전 (precharge) 제어기를 통해 선택적 매치라인 충전기법으로 CAM 동작 중 미스매치 상태에서 발생하는 전력 소모를 감소시켰고, 검색동작 중 미스매치가 발생했을 때 사전 충전을 조기 종료시킴으로써 단락 전류로 인한 전력 소모를 추가적으로 감소시켰다. 기존 회로와 비교했을 때, 전력 소모와 전파 지연 시간이 6.92%, 23.30% 감소하였고, PDP(: Product-Delay-Product)와 EDP(: Energy Delay Product)가 29.92%, 52.31% 감소하는 우수한 성능을 보였다. 제안한 회로는 TSMC 65nm CMOS 공정을 사용하여 구현되었으며 SPECTRE 시뮬레이션을 통해 그 타당성을 입증하였다.

• Structural Engineering and Mechanics
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• 제25권4호
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• pp.467-480
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• 2007

Two methods were developed for analyzing problems with two adjacent sub-domains modeled by different kinds of elements in finite element method. Each sub-domain can be defined independently without the consideration of equivalent division with common nodes used for the interface. These two methods employ an individual interface to accomplish the compatibility. The MLSA method uses the moving least square approximation which is the basic formulation for Element Free Galerkin Method to formulate the interface. The displacement field assumed by this method does not pass through nodes on the common boundary. Therefore, nodes can be chosen freely for this method. The results show that the MLSA method has better approximation than traditional methods.

• 한국어병학회지
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• 제32권2호
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• pp.69-80
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• 2019

We performed MLSA (multilocus sequence analysis) and phenotypic characterization of Aliivibrio species isolated from walleye pollock (Gadus chalcogrammus) maintained in 3 different facilities of Gangwon Province, the east coast of Korea. Of 38 Aliivibrio species identified by 16S rDNA sequences, 12 strains were randomly selected and MLSA was conducted with 5 house-keeping genes (gapA, gyrB, pyrH, recA and rpoA) and 16S rDNA gene. Phylogenetic analysis and homology of the concatenated sequences (4,580 bp) with other Vibrionaceae genera revealed that 4 strains (GNGc16.1, YYGc16.1, YYGc16.2, GSGc18.1) were identified as Aliivibrio logei and one strain (GSGc16.1) as A. wodanis. One strain (GSGc17.1) was tentatively identified as A. logei, but needs further analysis because it did not belong to the same clade with A. logei type strain. 6 strains (GSGc17.2, GNGc16.2, GSGc16.2, GSGc17.3, GSGc18.2, GSGc17.4) need further investigation as potential novel species. Either phenotypic characterization or 16S rDNA sequence alone did not provide enough information for identification of Aliivibrio strains at the species level. A. logei and A. wodanis are generally known as non-pathogenic bacteria, but also known as opportunistic or secondary pathogens of cold water fishes. Cares should be taken to prevent potential outbreaks due to these bacteria, although there was no outbreaks during the sampling period.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권11호
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• pp.4050-4067
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• 2014

In wireless sensor networks, link scheduling is a fundamental problem related to throughput capacity and delay. For a given set of communication requests $L=\{l_1,l_2,{\cdots},l_n\}$, the MLS (maximum link scheduling) problem aims to find the largest possible subset S of Lsuch that the links in S can be scheduled simultaneously. Most of the existing results did not consider bidirectional transmission setting, which is more realistic in wireless sensor networks. In this paper, under physical interference model SINR (signal-to-noise-plus-interference-ratio) and bidirectional transmission model, we propose a constant factor approximation algorithm MLSA (Maximum Link Scheduling Algorithm) for MLS. It is proved that in the same topology setting the capacity under unidirectional transmission model is lager than that under bidirectional transmission model. However, compared with some work under unidirectional transmission model, the capacity of MLSA is improved about 28% to 45%.

• Genomics & Informatics
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• 제11권4호
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• pp.272-276
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• 2013

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species.

• 한국어병학회지
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• 제33권2호
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• pp.127-138
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• 2020

코끼리조개 성패 및 유생으로부터 잠재적 병원성 세균 분리 및 동정을 수행하였다. 분리된 균주는 분자생물학적 기법 및 생화학적 검사를 통해 동정하였으며, 명확한 동정 및 계통수 분석을 위해 16S rDNA와 하우스키핑 유전자 (pyrH, recA, rpoA)를 결합하여 분석하는 MLSA를 적용하였다. 총 141개의 균주가 분리되었으며, 건강한 성패에서는 10개, 수포 병변을 보이는 빈사 상태의 성패에서 52개, 유생에서 79개가 분리되었다. 이 중 빈사상태의 성패에서 46개, 유생에서 39개의 균주가 Vibrio 속 세균으로 동정되었으며, 나머지 균주들은 모두 일반해양세균으로 동정되었다. Vibrio 속 세균 중에서는 수포 병변을 보이는 성패에서 Vibrio splendidus가 가장 많이 분리되었으며 기존의 V. splendidus와 함께 동일한 클러스터를 형성하였다. 하지만, 인위감염실험을 실시하지 않았고 건강한 유생에서도 V. splendidus가 분리되어 성패에서 나타난 수포와 V. splendidus와의 관계는 확실하지 않으며, 차후 추가 연구가 필요할 것으로 생각된다.

• 대한음성학회:학술대회논문집
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• 대한음성학회 2007년도 한국음성과학회 공동학술대회 발표논문집
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• pp.217-220
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• 2007

This study outlines a small-sized dialog style ETRI Korean TTS system which applies a HMM based speech synthesis techniques. In order to build the VoiceFont, dialog-style 500 sentences were used in training HMM. And the context information about phonemes, syllables, words, phrases and sentence were extracted fully automatically to build context-dependent HMM. In training the acoustic model, acoustic features such as Mel-cepstrums, logF0 and its delta, delta-delta were used. The size of the VoiceFont which was built through the training is 0.93Mb. The developed HMM-based TTS system were installed on the ARM720T processor which operates 60MHz clocks/second. To reduce computation time, the MLSA inverse filtering module is implemented with Assembly language. The speed of the fully implemented system is the 1.73 times faster than real time.

• The Plant Pathology Journal
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• 제34권4호
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• pp.269-285
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• 2018

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.