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http://dx.doi.org/10.5423/PPJ.OA.01.2018.0014

Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil  

Ferraz, Helvio Gledson Maciel (Department of Phytopathology, Universidade Federal de Vicosa)
Badel, Jorge Luis (Department of Phytopathology, Universidade Federal de Vicosa)
da Silva Guimaraes, Lucio Mauro (Department of Phytopathology, Universidade Federal de Vicosa)
Reis, Bruna Paolinelli (Department of Phytopathology, Universidade Federal de Vicosa)
Totola, Marcos Rogerio (Department of Microbiology, Universidade Federal de Vicosa)
Goncalves, Rivadalve Coelho (EMBRAPA Acre)
Alfenas, Acelino Couto (Department of Phytopathology, Universidade Federal de Vicosa)
Publication Information
The Plant Pathology Journal / v.34, no.4, 2018 , pp. 269-285 More about this Journal
Abstract
Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.
Keywords
Eucalyptus spp.; fatty acid profile; metabolism fingerprinting; multilocus sequence analysis; Xanthomonas axonopodis;
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