• Convergence Security Journal
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• v.7 no.4
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• pp.107-114
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• 2007

We need development methodology of security architecture which offered various levels of security management in case of the organization required more than two security certifications. In this study, therefore, development methodology of Multi-level Security Architecture(MLSA) proposed. Specifically, we should consider factors of commonness and difference between information security management level evaluation of multiple security architecture. This kinds of endeavor can suggest the direction of the improvement of the evaluating security management and the dynamic plan for the security architecture, and it will make the continuous and systematic security management.

• The Plant Pathology Journal
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• v.37 no.3
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• pp.243-257
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• 2021

Bacillus pumilus is the causal agent of trunk bulges disease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic relationships of 20 isolates of B. pumilus group from four rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang). Multi rep-PCR results revealed the genetic profiling among the B. pumilus group isolates, while MLSA results showed 98-100% similarity across the 20 isolates of B. pumilus group species. These 20 isolates, formerly established as B. pumilus, were found not to be grouped with B. pumilus. However, being distributed within distinctive groups of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates close to B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. This is the first MLSA and rep-PCR study on B. pumilus group, which provides an in-depth understanding of the diversity of these rubber-pathogenic isolates in Malaysia.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.5
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• pp.769-776
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• 2023

In this paper, we designed an MLSA(Match-line Sense Amplifier) for low-power CAM(Content Addressable Memory). By using the MLSA and precharge controller, we reduced power consumption during CAM operation by employing a selective match-line charging technique to mitigate power consumption caused by mismatch. Additionally, we further reduced power consumption due to leakage current by terminating precharge early when a mismatch occurs during the search operation. The designed circuit exhibited superior performance compared to the existing circuits, with a reduction of 6.92% and 23.30% in power consumption and propagation delay time, respectively. Moreover, it demonstrated a significant decrease of 29.92% and 52.31% in product-delay-product (PDP) and energy-delay-product (EDP). The proposed circuit was validated using SPECTRE simulation with TSMC 65nm CMOS process.

• Structural Engineering and Mechanics
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• v.25 no.4
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• pp.467-480
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• 2007

Two methods were developed for analyzing problems with two adjacent sub-domains modeled by different kinds of elements in finite element method. Each sub-domain can be defined independently without the consideration of equivalent division with common nodes used for the interface. These two methods employ an individual interface to accomplish the compatibility. The MLSA method uses the moving least square approximation which is the basic formulation for Element Free Galerkin Method to formulate the interface. The displacement field assumed by this method does not pass through nodes on the common boundary. Therefore, nodes can be chosen freely for this method. The results show that the MLSA method has better approximation than traditional methods.

• Journal of fish pathology
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• v.32 no.2
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• pp.69-80
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• 2019

We performed MLSA (multilocus sequence analysis) and phenotypic characterization of Aliivibrio species isolated from walleye pollock (Gadus chalcogrammus) maintained in 3 different facilities of Gangwon Province, the east coast of Korea. Of 38 Aliivibrio species identified by 16S rDNA sequences, 12 strains were randomly selected and MLSA was conducted with 5 house-keeping genes (gapA, gyrB, pyrH, recA and rpoA) and 16S rDNA gene. Phylogenetic analysis and homology of the concatenated sequences (4,580 bp) with other Vibrionaceae genera revealed that 4 strains (GNGc16.1, YYGc16.1, YYGc16.2, GSGc18.1) were identified as Aliivibrio logei and one strain (GSGc16.1) as A. wodanis. One strain (GSGc17.1) was tentatively identified as A. logei, but needs further analysis because it did not belong to the same clade with A. logei type strain. 6 strains (GSGc17.2, GNGc16.2, GSGc16.2, GSGc17.3, GSGc18.2, GSGc17.4) need further investigation as potential novel species. Either phenotypic characterization or 16S rDNA sequence alone did not provide enough information for identification of Aliivibrio strains at the species level. A. logei and A. wodanis are generally known as non-pathogenic bacteria, but also known as opportunistic or secondary pathogens of cold water fishes. Cares should be taken to prevent potential outbreaks due to these bacteria, although there was no outbreaks during the sampling period.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.11
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• pp.4050-4067
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• 2014

In wireless sensor networks, link scheduling is a fundamental problem related to throughput capacity and delay. For a given set of communication requests $L=\{l_1,l_2,{\cdots},l_n\}$, the MLS (maximum link scheduling) problem aims to find the largest possible subset S of Lsuch that the links in S can be scheduled simultaneously. Most of the existing results did not consider bidirectional transmission setting, which is more realistic in wireless sensor networks. In this paper, under physical interference model SINR (signal-to-noise-plus-interference-ratio) and bidirectional transmission model, we propose a constant factor approximation algorithm MLSA (Maximum Link Scheduling Algorithm) for MLS. It is proved that in the same topology setting the capacity under unidirectional transmission model is lager than that under bidirectional transmission model. However, compared with some work under unidirectional transmission model, the capacity of MLSA is improved about 28% to 45%.

• Genomics & Informatics
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• v.11 no.4
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• pp.272-276
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• 2013

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species.

• Journal of fish pathology
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• v.33 no.2
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• pp.127-138
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• 2020

We attempted to isolate and identify potentially pathogenic bacteria from geoduck clam (Panopea japonica) larvae, juvenile and adult, focusing on Vibrios. The isolates were identified by molecular approach and biochemical characterization. In particular, we applied MLSA (multilocus sequence analysis) to the isolated Vibrios for clear identification and phylogenetic relationships, by combining 16s rDNA and several houskeeping genes (pyrH, recA, rpoA). We obtained 141 isolates; 10 from healthy adults, 52 from moribund adults with blisters and 79 from larvae. 46 from the moribund adults and 39 from the larvae were identified as Vibrio species, while the rest of these samples and all the isolates from healthy adult were identified as marine general bacteria. Among Vibrio species, Vibrio splendidus was the most frequently identified from the moribund adults and clustered with the known V. splendidus in GenBank by MLSA. However, it was still unclear that V. splendidus was the cause of blisters because the artificial infection experiment was not conducted and V. splendidus was isolated also from the larvae. Further studies are necessary to clarify the etiological agent of the blisters found in geoduck clam in this study.

• Proceedings of the KSPS conference
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• 2007.05a
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• pp.217-220
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• 2007

This study outlines a small-sized dialog style ETRI Korean TTS system which applies a HMM based speech synthesis techniques. In order to build the VoiceFont, dialog-style 500 sentences were used in training HMM. And the context information about phonemes, syllables, words, phrases and sentence were extracted fully automatically to build context-dependent HMM. In training the acoustic model, acoustic features such as Mel-cepstrums, logF0 and its delta, delta-delta were used. The size of the VoiceFont which was built through the training is 0.93Mb. The developed HMM-based TTS system were installed on the ARM720T processor which operates 60MHz clocks/second. To reduce computation time, the MLSA inverse filtering module is implemented with Assembly language. The speed of the fully implemented system is the 1.73 times faster than real time.

• The Plant Pathology Journal
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• v.34 no.4
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• pp.269-285
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• 2018

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.