• Smart Structures and Systems
• /
• v.7 no.3
• /
• pp.213-222
• /
• 2011

The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.16 no.6
• /
• pp.1075-1088
• /
• 1992

This paper presents techniques used to model a two arm experimental robot. Both arms are compliant and the robot operates in a vertical plane and is therefore influenced by gravity. The robot is being built to study different control strategies for robots containing compliant members. The system is built with extremely flexible members. This limits the required bandwidth of the control electronics, and mimics the flexible motions that are observed for stiffer faster robots. The objective of this paper is to develop a reduced order model of the robot system and to experimentally validate the model. Validation requires that the model includes gravitational effects. Therefore, an assumed modes model is developed which facilitates modeling of gravitational effects. In order to select the order and mode shapes for the model, an analytical solution is derived for a linearized continuous model. This is compared to the assumed modes model to determine the number of mode shapes needed to model the system. The final model, which includes shortening effects, correlates very well with experimental results.

• Journal of Microbiology
• /
• v.44 no.2
• /
• pp.217-225
• /
• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.

• The Journal of Korean Orthopaedic Ultrasound Society
• /
• v.2 no.1
• /
• pp.37-44
• /
• 2009

Prolotherapy, the technology for strengthening lax ligaments, has found increased acceptance in recent years. Prolotherapy involves the injection of the irritant solutions into the weakened or stretched ligaments which are a source of chronic pain. This induces an inflammatory response which mimics the normal repair sequence. Various musculoskeletal disorders of lower extremities such as degenerative arthrtitis, ligament and tendon injuries can be treated with prolotherapy. Prolotherapy is a very good, powerful nonsurgical conservative treatment in various musculoskeletal disorders. Ultrasound exam is very useful to find and confirm the exact location of disorders and check the progress in prolotherapy practices.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2003.05a
• /
• pp.77-81
• /
• 2003

SDM (Structural Dynamics Modification) is a tool to improve dynamic characteristics of a structure, more specifically of a base structure, by adding or deleting auxiliary (modifying) structures. In this paper, the goal of the optimal SDM is set to maximize the natural frequency of a base plate structure by attaching serially-connected beam stiffeners. The design variables are chosen as positions of the attaching beam stiffeners, where the number of stiffeners is considered as a design space. The problem of non-matching interface nodes between the base plate and beam stiffeners is solved by using localized Lagrange multipliers, which act to glue the two structures with non-matching interface nodes. As fer the cases of non-matching interface nodes problem, the governing equation of motion of a structure can be considered from the viewpoint of a topological modification, which involves the change of the number of structural members and DOFs. Consequently, the eigenpairs of the beam-stiffened plate structure are obtained by using an eigen reanalysis technique of topological modifications. Evolution Strategies (ES), which is a probabilistic population-based optimization technique that mimics the principles from biological evolution in nature, is utilized as a mean for the optimization.

• Journal of Periodontal and Implant Science
• /
• v.45 no.1
• /
• pp.8-13
• /
• 2015

Purpose: The aim of this study was to evaluate the transfer of different occlusal forces in various skeletal malocclusions using finite element analysis (FEA). Methods: Three representative human cone-beam computed tomography (CBCT) images of three skeletal malocclusions were obtained from the Department of Orthodontics, Yonsei University Dental Hospital, Seoul, South Korea. The CBCT scans were read into the visualization software after separating bones and muscles by uploading the CBCT images into Mimics (Materialise). Two separate three-dimensional (3D) files were exported to visualize the solid morphology of skeletal outlines without considering the inner structures. Individual dental impressions were taken and stone models were scanned with a 3D scanner. These images were integrated and occlusal motions were simulated. Displacement and Von Mises stress were measured at the nodes of the FEA models. The displacement and stress distribution were analyzed. FEA was performed to obtain the 3D deformation of the mandibles under loads of 100, 150, 200, and 225 kg. Results: The distortion in all three skeletal malocclusions was comparable. Greater forces resulted in observing more distortion in FEA. Conclusions: Further studies are warranted to fully evaluate the impact of skeletal malocclusion on masticatory performance using information on muscle attachment and 3D temporomandibular joint movements.

• Bulletin of the Korean Chemical Society
• /
• v.32 no.10
• /
• pp.3702-3706
• /
• 2011

The conformation of a neuropeptide, substance P (SP), in isotropic (q = 0.5) acidic bicelles was investigated using two-dimensional NMR techniques. By the nuclear Overhauser effect (NOE) cross peaks between SP and long-chain lipid molecules SP was probed to bind on the flat surface of the disc-like bicelles. Structural analysis of NMR data indicated that the helical conformation of SP extended to the C-terminal region of Leu10 as well as in the mid-region from Pro4 to Phe8. As compared with the conformations of SP bound on the sodium dodecylsulfate (SDS) or the dodecylphosphocholine (DPC) micelles with curved surfaces, the surface curvature of the membrane mimics was found to be one of the major factors inducing the biologically relevant conformation of SP. The negative surface charge of the membrane is also a key factor inducing both the binding of SP on the membrane and its biologically active structure.

• BMB Reports
• /
• v.47 no.12
• /
• pp.666-672
• /
• 2014

The Wnt signaling pathway is well known to play major roles in skeletal development and homeostasis. In certain aspects, fracture repair mimics the process of bone embryonic development. Thus, the importance of Wnt signaling in fracture healing has become more apparent in recent years. Here, we summarize recent research progress in the area, which may be conducive to the development of Wnt-based therapeutic strategies for bone repair.

• BMB Reports
• /
• v.50 no.7
• /
• pp.373-378
• /
• 2017

The Jun activation-domain binding protein 1 (Jab1) induces p53 nuclear export and cytoplasmic degradation, but the underlying mechanism is poorly understood. Here, we show that phosphorylation at the threonine 155 residue is essential for Jab1-mediated p53 nuclear export. Jab1 stimulated phosphorylation of p53 at T155 was inhibited by curcumin, an inhibitor of COP9 signalosome (CSN)-associated kinases. The T155E mutant, which mimics phosphorylated p53, exhibited spontaneous cytoplasmic localization in the absence of Jab1. This process was prevented by leptinomycin B (LMB), but not by curcumin. The substitution of threonine 155 for valine (T155V) abrogated Jab1-mediated p53 nuclear export, indicating that phosphorylation at this site is essential for Jab1-mediated regulation of p53. Although T155E can be localized in the cytoplasm in the absence of Mdm2, the translocation of T155E was significantly enhanced by ectopic Hdm2 expression. Our data suggests that Jab1-mediated phosphorylation of p53 at Thr155 residue mediates nuclear export of p53.

• 제어로봇시스템학회:학술대회논문집
• /
• 1998.10a
• /
• pp.195-200
• /
• 1998

Since the neural network was introduced, significant progress has been made on data handling and learning algorithms. Currently, the most popular learning algorithm in neural network training is feed forward error back-propagation (FFEBP) algorithm. Aside from the success of the FFEBP algorithm, polynomial neural networks (PNN) learning has been proposed as a new learning method. The PNN learning is a self-organizing process designed to determine an appropriate set of Ivakhnenko polynomials that allow the activation of many neurons to achieve a desired state of activation that mimics a given set of sampled patterns. These neurons are interconnected in such a way that the knowledge is stored in Ivakhnenko coefficients. In this paper, the PNN model has been developed using the plasma enhanced chemical vapor deposition (PECVD) experimental data. To characterize the PECVD process using PNN, SiO$_2$films deposited under varying conditions were analyzed using fractional factorial experimental design with three center points. Parameters varied in these experiments included substrate temperature, pressure, RF power, silane flow rate and nitrous oxide flow rate. Approximately five microns of SiO$_2$were deposited on (100) silicon wafers in a Plasma-Therm 700 series PECVD system at 13.56 MHz.