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Development of Disease-resistant Japonica Rice Varieties and Effects of Pyramiding Resistance Genes (내병성 자포니카 벼 계통 육성과 저항성 유전자 집적효과)

  • Kim, Woo-Jae;Baek, Man-Kee;Park, Hyeon-Su;Lee, Geon-Mi;Lee, Chang-Min;Kim, Seok-Man;Cho, Young-Chan;Seo, Jeong-Phil;Jeong, O-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.314-326
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    • 2020
  • This study was carried out to develop a resistant variety against the K3a race of bacterial blight, Xanthomonas oryzae pv. oryzae, through expansion and pyramiding of resistance genes. To develop an elite bacterial blight-resistant cultivar, the breeding process and bacterial blight resistance reactions in advanced backcross lines (ABLs) were analyzed. ABLs21 which contain Xa3 and Xa21, were developed by double backcrossing japonica cultivar Hwanggeumnuri, which has bacterial blight resistant Xa3 gene, and indica variety IRBB21, which havs Xa21 gene, followed by disease resistance bioassay and marker-assisted selection. The resistance genes of ABLs21 were amplified by PCR with the molecular markers 9643.T4 (Xa3) and U1/I1 (Xa21). Hwanggeumnuri and IRBB3 showed resistance reactions against K1, K2, and K3 races, and a susceptible reaction against K3a, K4, and K5 races. IRBB21 showed resistance reactions against K2, K3, K3a, K4 and K5 races, and a susceptible reaction against K1 race. Hwanggeumnuri showed susceptible reactions at the seedling, tillering and adult stages (all stages), whereas ABL21-1 showed moderate resistance at the tillering stage. ABL21-1 showed stable resistance against 18 isolates of K3a race, and the lesion length was shorter than that of the donor parents. In cluster analysis, the HB4032 isolate showed the highest pathogenicity among the 18 isolates. The molecular marker polymorphisms and average substituted chromosome segment lengths of ABLs21 were 63.2 % and 86.1 cM, respectively. Insertion of the donor chromosomal segments occurred in the predicted region of the Xa21 gene of ABLs21.

Database for Hospice Nursing in Electronic Medical Record (호스피스 전자기록을 위한 데이터베이스 개발)

  • Kim, Young-Soon;Lee, Chang-Geol;Lee, Kyoung-Ok;Kim, Ok-Kyum;Kim, In-Hye;Kim, Mi-Jeong;Hwang, Ae-Ran;Lee, Won-Hee
    • Journal of Hospice and Palliative Care
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    • v.7 no.2
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    • pp.200-213
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    • 2004
  • Purpose: The purpose of this study was to create an electronic nursing record form to build a hospice nursing process database to be used in the u-hospital EMR system. Specific aims of the study were: 1. To generate a complete, accurate, and simple electronic nursing record form. 2. To verify its appropriateness following documentation with the standardized hospice protocol. 3. To verify its validity and finalize the hospice nursing process database through discussion among hospice professionals. Methods: Nursing records from three independent hospice organizations were collected and analyzed by five expert hospice nurses with more than 10 years of experience, and a nursing record database was developed. This database was applied to 81 hospice patients at three hospice organizations to verify its completeness. Results: 1. An electronic nursing record form with completeness, accuracy, and simplicity was developed. 2. The completeness of the standardized home hospice service protocol was 95.86 percent. 3. The hospice nursing process database contains 18 items on health problems, 79 items on related causes and major symptoms, and 229 items on nursing interventions. Conclusion: The new nursing record form and database will reduce documentation time and articulate and streamline the working process among team members. They can also improve the quality of hospice services, and ultimately enable us to estimate hospice service costs.

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A Survey of the Basic Protection Manners of Dental Personnels Against Hepatitis B in and around Seoul (수도권 치과의료 종사자들의 B형 간염에 대한 기초방호실태 및 수행에 관한 조사)

  • Yoon, Mi-Sook;Park, Mi-Young
    • Journal of dental hygiene science
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    • v.1 no.1
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    • pp.46-52
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    • 2001
  • The purpose of this study was to examine dental personnels' concern for health, their personal protection manner, and whether or not they were inoculated against hepatitis B and made an inquiry of patients about that. It's ultimately meant to serve as a basis for the preparation of an anti-infection guide for the protection of dental personnels against all sorts of infectious diseases. After a survey was conducted from March 29 through May 9, 2001, the following findings were obtained: (1) The dental personnel group that had worked for 5 to less than 7 years took health examination the most, with 71.4%, before joining the hospital, and 61.5% of those who had served for 9 years or more, the greatest percentage, took medical checkup after joining the hospital(P<0.05). (2) The largest percentage of being inoculated against the anti-B type hepatitis after employment was 71.4% of the dental personnels over 35, and 57.7% of those with 9-year or more working experiences. Therefore, their age and service term made a significant difference(P<0.05). (3) There was no significant gap in personal protection practices between the dental hygienists and nurse aids(P>0.05). But the use of safety goggles for treatment was not properly done, with just 1.48 on the basis of 3 points, whereas the use of mask was scored 2.40 and the use of gloves was scored 1.96. (4) After a narcotic was used once, the leftovers were wasted by 89.7% of the dental hygienists and 70.0% of the nurse aids. More dental hygienists wasted them(P<0.05). (5) For hand washing, antibiotic liquid soap was used by 19.3% of the dental hygienists and 10.0% of the nurse aids, which were both very low percentage. And just 37.2% of the dental hygienists utilized a disposable paper towel to dry their hands, and only 36.0% of the nurse aids used the same(P<0.05).

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Effects of Flavonoid from Rhus verniciflua on Testosterone Secretion by Rat Leydig Cells In Vitro (옻나무 유래 Flavonoid 처리가 흰쥐 Leydig 세포의 체외배양에서 Testosterone 분비에 미치는 영향)

  • 성환후;최선호;장유민;민관식;우제현;장원경;정남철;나천수;정일정
    • Korean Journal of Animal Reproduction
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    • v.25 no.2
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    • pp.125-130
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    • 2001
  • This study was performed to report a direct dose dependent stimulatory effect of the Flavonoid(F) on basal testosterone secretion and a dose dependent effect on LH induced testosterone production by Leydig cell of matured rats in vitro culture. F was obtained kom the Rhus vernicifua through aceton extraction and silica gel adsorption column chromatography. Leydig cells (1$\times$10$^{6}$ cells/well) from 12 weeks old rats were incubated with or without F(0, 20, 40, 80, 160 ng) or insulin-like growth factor-I(IGF-I) in the presence or absence of LH(10, 100ng). 1. The maximal stimulatory concentrations of testosterone in culture media were showed at 24hr of culture. but these testosterone level were decreased at 36 hr of culture. 2. Flavonoid(80ng) were significantly(P < 0.05) increased testosterone production compared with control groups for 12 hr culture. 3. Testosterone secretion by Leydig cells stimulated with LH(10, 100ng) for 6 hr and 12hr culture compared with 3 hr culture. 4. LH 10 ng augmented testosterone were increased by addition of F 40 ng for 12 hr culture. 5. F(0 and 40 ng) also enhanced LH 10 ng stimulated testosterone for 3 hr Leydig cells culture. 6. Addition of IGF-I 100 ng to the culture medium for 6 hr were increased the concentration of testosterone by Leydig cells stimulated with 100 ng LH. These results indicate that Flavonoid has a direct stimulatory effect on basal testosterone secretion in rat Leydig cells, and also modulates LH mediated testosterone. Therefore, Flavonoid may act as a modulator on gonadal development or gonadal steroidogenesis in direct or indirect.

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HYDROLYTIC DEGRADATION OF POSTERIOR RESIN RESTORATIVE MATERIALS (구치부 레진 수복 재료의 가수분해)

  • Yang, Kuy-Ho;Park, Mi-Ran;Choi, Nam-Ki;Park, Eun-Hae
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.4
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    • pp.673-682
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    • 2001
  • The use of resin composites has continued to increase over the last several years. In spite of their growing popularity, composites continue to exhibit a number of undesirable characteristics. One of the major deficiencies of composite restorative resins is their inadequate resistance to wear. Of the multitude of factors that have been associated with wear, subsurface degradation within the restoration is considered to be one. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. This solution with a high concentration of hydroxyl ions is a convenient medium for accelerated degradation of silane coupling and filler particles. The brands studies were Definite($Degussa-H\ddot{u}ls$ AG, Germany), Prodigy(Kerr, USA), Pyramid(Bisco, USA) and Synergy(Coltene, Swiss). Preweighed discs of each brand were exposed to 0.1N NaOH solution at $60^{\circ}C$. After 14 days they were removed, neutralized with HCl, washed with water and dried. Resistance to degradation was evaluated on the basis of following parameters : (a) mass loss(%)-determined from pre-and post-exposed specimen weights : (b) Si loss(ppm)-obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation $depth({\mu}m)$-measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows: 1. Mass loss of Synergy was $1.24{\pm}0.002%$, it was the highest, there was no significant difference among the materials. 2. The degree of degradation layer depth of Synergy was $107.83{\pm}2.52{\mu}m$, it was the highest, there was no significant difference among any other materials than Synergy. 3. There was no difference among the four materials in Si loss. 4. The correlation coefficient between mass loss and degradation depth was relatively high(r=0.06, p<0.05). 5. There was no coefficient correlation between Si loss and mass loss, the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler.

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An Analysis of Sexual Health Education for Korean Adolescents (청소년대상 영상매체 성교육자료분석 -비디오를 중심으로-)

  • Lee, Koang-Ok;Yang, Soon-Ok;Im, Mi-Lim
    • Research in Community and Public Health Nursing
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    • v.9 no.2
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    • pp.441-457
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    • 1998
  • The purpose of this study is to provide fundamental information for the development of new sex education image media through the analysis of those tools produced by Planned Parenthood Federation of Korea. Twenty three video tapes related to the sex education were analysed. The data were sampled on the basis of the sex education instructions for primary and secondary schools published by the Seoul Office of Education in 1996 and the list of sex education information composed by the Planned Parenthood Federation of Korea with the following requirements. 1) The education materials are oriented to the middle and high school students as well a juveniles and are being used in public health centers and schools. 2) The education materials are not confined to the single subject such as AIDS or contraception. Because this study is focused on the analysis of image media, an analysis method widely and effectively used in public media study was chosen. The frequency analysis and percentage treatment were done by an SAS program. The results of the study are as follows : 1. An analysis of the material structure type showed that the frequency of the following subjects in 15 video tapes, composed as drama and cartoon, had this decreasing orde : sex behavior, sex relation ships, sex health, human development, personal technique, and society and culture. 2. The eight tapes of explanation and counseling showed a different sequence from that of the drama and cartoon material, primarily treating human development with 5419 seconds (47.9%) of type. The following were also considered in decreasing order: at 2000seconds (17.6%) sex behavior, at 1366 seconds (12.1%) sex health, at 1240 seconds (11.0%) society and culture, at 667 seconds (6.0%) relation ships, and at 611 seconds (5.4%) personal technique. 3. In a thematic analysis those education materials dealing with human development show this frequency sequence: anatomic physiology, reproduction, and adolescence. In relation, ships the sequence is dates, love, marriage and vows, and family. For personal technique, it is expressed as asking for help, and an empnasis on moral values. For sex behavior, they focus on abstinence, the co-responsibility of sex behavior, the sexual response of the human, and sexual nightmares. For the sex health area, the focus is contraception with abortion and reproductive health following that. In society and culture diversity is considered. 4. An analysis of the differences in production and content between 1980s and 1990s was also done. For materials produced in the 1980s the frequency of the subjects follows this order: sex behavior, human development, relation ships, sex health, personal technique, and society and culture with sub subjects such as abstinence, dating, adolescence, anatomic physiology, reproduction, calls for help, and the sexual response of a human being. For materials produced in the 1990s, highest frequency was sex behavior, the same as the 1980s. The rest of the material follows this decreasing order: relationships, human development, sex health, society and culture, and personal technique with sub subjects such as dating, co-resposibility in sex behavior, abstinence, and love frequently considered.

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Immuno-modulatory and Antitumor Effect of Crude Polysaccharides Extracted from Sclerotium of Grifola umbellata (저령(Grifola umbellata)의 균핵에서 추출한 조다당류의 면역활성 및 항암 효과)

  • Oh, Yun-Hee;Lee, U-Youn;Lee, Min-Woong;Shim, Mi-Ja;Lee, Tae-Soo
    • The Korean Journal of Mycology
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    • v.32 no.1
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    • pp.23-30
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    • 2004
  • Neutral salt-soluble, hot water-soluble and methanol-soluble materials (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from sclerotium of Grifola umbellata. The Fr. NaCl and Fr. HW did not show any direct cytotoxicity against NIH3T3, Sarcoma 180 and MCF-7, but Fr. MeOH showed cytotoxicity against these cell lines at the concentration of $1,000\;{\mu}g/ml$. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 66.7% and decrease the number of Sarcoma 180 cells of 54.2% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity through alternative complement pathway and the alkaline phosphatase activity by $85.05{\sim}88.73%$ and 6 folds, respectively. The number of peritoneal exudate cells and the circulating leucocytes were increased by 1.7 and 3.6 folds in the Fr. NaCl treating group compared with the control group, respectively. The weight of immunoorgans such as liver, spleen and thymus were also gradually increased. The hematological analysis of the Fr. NaCl group was similar with that of the control group. The total polysaccharide and protein contents of Fr. NaCl were 98.25% and 1.44%, respectively. These results indicate that the antitumor activity of Fr. NaCl was exerted through immunopotentiation, but not through cytotoxicity against the tumor cells.

A Comparison of the Sensory Processing of Children With and Without Spastic Diplegia (경직형 양하지 마비 아동과 일반아동의 감각처리 능력 비교)

  • Kim, Bo-Ram;Shin, Ji-Eun;Kim, Kyeong-Mi
    • The Journal of Korean Academy of Sensory Integration
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    • v.13 no.2
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    • pp.21-29
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    • 2015
  • Objective : This study compares sensory processing ability of children with and without spastic diplegia. In addition, it investigates characteristics of sensory processing differentiated by developmental phase such as pre-school age versus school age. Methods : Participants in this study are ordinal children without specific condition and children with spastic diplegia who are aged 3 to 10 years olds. Using Short Sensory Profile (SSP), sensory processing function of the participants was measured. The survey was distributed to caregivers of the children from November, 2013 to February, 2014, and it was suggested that the caregivers to record the questionnaire directly after approval from a rehabilitation hospital, a university hospital, welfare center, day care center, preschool and elementary school to participate in our study. Results : Group of children with spastic diplegia showed lower score than group of children with no special condition in the total score and the each score of all items of Short Sensory Profile. There was significant difference between the two groups in terms of the total score of sensory processing and the 5 factors except tactile sensitiveness and taste/smell sensitiveness among the 7 factors of test. In the comparison of different age groups, pre-school age group showed lower total score than school age group. Conclusion : This study provides a foundational evidence that can be used when therapist evaluate sensory processing function of children with spastic diplegia. There is need for more study about sensory processing functions of various types of children with cerebral palsy.

The Effect of the Periods of Bed Rest on the Postlumbar Puncture Headache in Pediatric Oncology Patients (소아혈액 종양환자의 요추천자 후 침상안정시간이 천자 후 두통에 미치는 영향)

  • Kim, Yeong-Mi
    • The Korean Nurse
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    • v.36 no.2
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    • pp.73-83
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    • 1997
  • Bed rest is recommended to prevent postlumbar puncture headaches(PLPHA), but the period of bed rest varies in the literature from 6 hours to 24 hours. In clinical practice the period of bed rest varies but nursing methods for adults and children have little difference. In Seoul National University Hospital, children have been given at least 6 hours bed rest after a lumbar puncture. Pediatric oncology patients require a lumbar puncture for an initial diagnosis, follow up treatment or administration of chemotherapeutic agent. But it is difficult for young children to lie supine or to refrain from their usual activities in any way, and unpleasant problems related to a shortage of beds often occurs during discharge or in an outpatient setting. The purpose of this study is to substantiate the preventive effect of PLPHA by the period of bed rest, to identify the other factors that influence PLPHA, and to use the nursing methods proper to children. The subjects were 65 children, ages 1-17, undergoing treatment in the children's cancer center at SNUCH during the period June 1, 1995, to Aug. 31, 1995. The team nurses asked questions about PLPHA of the parents and children in order to fill out a questionnaire. The data were evaluated by percent, t-test, Chi-square test and Mann-Whitney U test. Result; 1. There was no significant difference relating the bed rest time spent to the occurrence of postspinal headaches (t-test). 2. There was a significant risk of PLPHA in the children who were irritable before procedure and/or had experienced previous PLPHA(p<0.05, ${x^2}-test$). 3. The following factors were not found to be associated with increased risk of PLPHA: previous puncture experience, giving analgesics, the choice of puncturist, inpatient/outpatient status, gauge of needle, purpose, the amount of CSF removed, gender, diagnosis, the number of peripheral WBCs, previous lumbago experience after LP, position after bed rest, age, the number of aural puncture at the time. A longer period of bed rest is unlikely to be more effective to prevent PLPHA and seems impractical. A shorter period will save time and effort. Perhaps it will also allay some of the fears which surround LP. So 1 hour bed rest after LP is suggested and nursing methods for emotional support should be investigated to reduce PLPH.

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The Vegetation Characteristics of Palustrine Wetland by Land-use in Rural Areas (농촌지역 소택지의 토지이용별 식생특성)

  • Son, Jin-Kwan;Kim, Mi-Heui;Kang, Banghun
    • Journal of Wetlands Research
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    • v.14 no.4
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    • pp.699-713
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    • 2012
  • Palustrine wetland is often used for development work and green area creation. However, the ecological characteristic of vegetation is not frequently considered in planning. Therefore, this study was conducted to investigate vegetation characteristic according to land-use of palustrine wetland and to provide basic materials in assessment of palustrine wetland and restoration and creation of wetland. Survey sites were caterogized into paddy, field, and mountain. Each category had two sites, so total 6 survey sites were investigated for vegetation. As a result, with a range of 61~92 species, total 216 taxa were confirmed, consisting of 71 families, 168 generic, 187 species, 2 variety 1 forma 1 subforma. Mountain received a high score in the evaluation score range of floristic regional indicator plants, and it was found that there were 10 families and 28 species of naturalized plant. Mountain showed low appearance, and fiddy and field showed relatively high appearance. Naturalized plants with slightly high vegetation cover ratio are Erigeron annuus, Amorpha fruticosa, Dactylis glomerata, Festuca arundinacea, Eragrostis curvula, Trifolium repens, Erigeron Canadensis, Ambrosia trifida, Oenothera odorata, Bidens frondosa, etc. Such naturalized plants should be removed to prevent terrestrialization of wetland and its harmful damages. Long-term monitoring and proper management of naturalized plants are expected to improve palustrine wetland into high quality biotope of rural area in the future.