• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.102-108
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• 2004

The present experiment was conducted to assess the efficacy of methionine hydroxy analogue free fatty acid (MHAFA) in comparison to DL-methionine (DL-Met) utilizing day-old commercial broiler chicks (n=300). The chicks were randomly distributed into 30 groups of 10 chicks each. Three dietary treatments, viz. D1-maize-soybean meal based basal diet (Control), D2- control diet supplemented with DL- methionine to meet its requirements and D3-control supplemented with MHA-FA @ 1.54 times of DL-methionine used in D2, were formulated. Each dietary treatment was offered to 10 replicated groups of chicks following completely random design (CRD). The chicks fed on supplemental DL-Met had significantly higher (p<0.01) gain in body weight, followed by MHA-FA group and control during 0-3 weeks of age. During overall growth period (0-6 weeks), chicks in DL-Met and MHA-FA groups grew better (p<0.01) than those in control. Feed conversion ratio (FCR) improved (p<0.01) on supplementation of either DL- Met or MHA-FA in the basal (Control) diet during 0-3 weeks of age. The FCR for overall period, however, did not differ statistically (p>0.05) amongst the treatments. The eviscerated yields emanated from diets with DL-Met or MHA-FA were higher (p<0.01) than that in Control. Abdominal fat pad was also more (p<0.01) in broilers fed control diet than in DL-Met or MHA-FA supplemented group. Breast yield was higher (p<0.05) in MHA-FA fed broilers than those fed DL-methionine supplemented or un-supplemented diets. The efficacy of MHA-FA in comparison to DL-Met for growth was 62.11, 64.82 and 63.88% and for feed efficiency was 62.98, 67.73 and 64.01% at 0-3, 3-6 and 0-6 weeks of age, respectively, while it was 65.85, 71.40 and 67.49% for eviscerated yield, abdominal fat pad reduction and breast yield at 6 weeks of age, respectively.

• Nutrition Research and Practice
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• 제9권2호
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• pp.144-149
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• 2015

BACKGROUND/OBJECTIVE: The aim of this study was to examine the effect of high dietary methionine (Met) consumption on plasma and hepatic oxidative stress and dyslipidemia in chronic ethanol fed rats. MATERIALS/METHODS: Male Wistar rats were fed control or ethanol-containing liquid diets supplemented without (E group) or with DL-Met at 0.6% (EM1 group) or 0.8% (EM2 group) for five weeks. Plasma aminothiols, lipids, malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase were measured. Hepatic folate, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured. RESULTS: DL-Met supplementation was found to increase plasma levels of homocysteine (Hcy), triglyceride (TG), total cholesterol (TC), and MDA compared to rats fed ethanol alone and decrease plasma ALT. However, DL-Met supplementation did not significantly change plasma levels of HDL-cholesterol, cysteine, cysteinylglycine, and glutathione. In addition, DL-Met supplementation increased hepatic levels of folate, SAM, SAH, and SAM:SAH ratio. Our data showed that DL-Met supplementation can increase plasma oxidative stress and atherogenic effects by elevating plasma Hcy, TG, and TC in ethanol-fed rats. CONCLUSION: The present results demonstrate that Met supplementation increases plasma oxidative stress and atherogenic effects by inducing dyslipidemia and hyperhomocysteinemia in ethanol-fed rats.

• 보건의료산업학회지
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• 제12권3호
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• pp.143-156
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• 2018

Objectives: The aim of this study was to determine the affecting factors of metabolic syndrome (MetS) and it's components in Korean adults in their 30s and 40s. Methods: The present study assessed 1,215 subjects who participated in the Korea National Health and Nutrition Examination Survey in 2014. We analyzed the prevalence rate of MetS and it's components and their affecting factors. Results: The prevalence rate of MetS was 16%. Family history of diabetes mellitus and, low or high saturated fat intake were associated with MetS in males. Low income, low education, excessive sleep, excessive carbohydrate and low saturated fat intake were associated with MetS in females. Triglycerides showed the highest prevalence in males and were related to white collar socioeconomic status, smoking, and obesity. Triglycerides and high-density lipoprotein cholesterol were high in females, and triglycerides were affected by excessive sleep, family history, low saturated fat intake, and obesity. High-density lipoprotein cholesterol was affected by obesity. Conclusions: Because sex differences exist in MetS, it is necessary to intervene through diverse approaches. In particular, a continuous management and preventive intervention for obesity is needed.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1992년도 제1회 신약개발 연구발표회 초록집
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• pp.61-61
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• 1992

여러 점막 추출액증 Met-Enk의 효소적 분해는 매우 신속하였고 1차식에 따랐으며 점막측 추출액에서의 분해속도는 직장>눈>질>비강점막의 순이었다. 비강, 직장, 질점막의 점막측 및 장막측 추출액 간의 분해속도에는 유의성 있는 차이는 없었으나 눈점막의 경우 내피측 추출액에서의 분해속도 (1.03 $\times$ $10^{-3}$ $min^{-1}$)가 표피측 추출액에서보다 현저히 낮았다. 모든 추출액에서 검출된 주요 분해산물은 Tyr 및 Des-Tyr-Met-Enk로 천연 Met-Enk의 점막 추출액중 분해는 주로 aminopeptidase류가 관여되고 있음을 알았으며 Tyr-Gly, Tyr-Gly-Gly등의 분해산물이 소량 출현함으로 보아 endopeptidase류도 분해에 다소 관여하고 있음을 알았다. YAGFM은 Met-Enk보다 분해가 현저히 지연되었으며 그 분해속도도 직장>질>비점막의 순이었다. 이러한 분해는 분해산물로 Tyr이 검출되지 않은점으로 보아 YAGFM의 점막 추출액중 분해는 주로 endopeptidase류가 주로 관여되는 것으로 추정된다.

• KIEAE Journal
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• 제16권1호
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• pp.11-19
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• 2016

Purpose: PMV, PET, and similar thermal comfort indices and microclimate modeling have recently become actively used to evaluate thermal comfort. This study will look at pedestrian roads with diverse spatial characteristics on university campus using the ENVI-met model as the base for onsite measurement. Method: The PET was used as the thermal comfort index. The first microclimate measures were collected on September 20, 2014, and the second microclimate measures were collected on June 1, 2015. The ENVI-met model was used at the same time. Result: As a results, Onsite measurement results differed depending on the PET spatial characteristics. The location associated with the most discomfort had a PET of $47.8^{\circ}C$. The spatial characteristics of this place included a with no shade. The most comfortable location had shade, and the PET was $24.6^{\circ}C$. When the ENVI-met model and onsite measurements were compared, similar patterns were found, but with a few differences at specific points; this was due to the limitation of using input materials such as trees, buildings, and covering materials with the ENVI-met model. This factor must be thoroughly considered when analyzing modeling results.

• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1547-1554
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• 2009

This study was carried out to evaluate the effects of dietary supplementation of different sources of chromium on growth performance, blood profile and carcass trait in growing-finishing pigs. A total of 200 growing pigs (Landrace${\times}$Yorkshire)${\times}$Duroc, average initial weight 8.5 kg) were allotted to 5 treatments with 4 replicates per treatment and 10 pigs per replicate. Five treatments were designated as follows according to the source of chromium. i) Control (No chromium): corn-soybean meal based basal diet, ii) $CrCl_{3}$: control diet+200 ppb Cr as $CrCl_{3}$, iii) CrPic: control diet+200 ppb Cr as Cr picolinate, iv) CrMet-1: control diet+100 ppb Cr as Cr methionine, and v) CrMet-2: control diet+200 ppb Cr as Cr methionine. After the feeding trial, three pigs per replicate (12 pigs per treatment) were slaughtered for the evaluation of carcass traits. Average daily gain (ADG), average daily feed intake (ADFI), and feed: gain ratio (F/G) were not different (p>0.05) among dietary Cr sources. However, whole-period ADG of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. Nutrient digestibility was not different (p>0.05) among dietary Cr sources, but the nutrient digestibility of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. BUN level decreased with more magnitude (p<0.05) in pigs fed Cr during the 20 to 50 kg period. Although both serum cholesterol and triglyceride were different (p<0.05) among treatments, there was no consistent response that could be related to the dietary Cr sources regardless of growth phase. However, the overall data suggested that serum cholesterol level increased as BW of pigs increased. Blood total protein (TP) increased (p<0.05) in pigs fed Cr only during the 90-110 kg phase, and blood creatinine (Creat) level was higher in $CrCl_{3}$ and CrPic treatments than in the control only during the 90-110 kg phase. Backfat thickness was thinner (p<0.05) in pigs fed CrMet-2 than in the control treatment. Therefore, lean percentage was higher (p<0.05) in CrMet-2 than in control pigs. However, dressing percentage and Longissimus muscle area (LMA) were not different (p>0.05) among treatments. In conclusion, dietary supplementation of 200 ppb Cr, via either CrPic or CrMet, improved pig growth performance and nutrient digestibility. Moreover, dietary CrMet supplementation for the growing-finishing pig is evidently remarkable for improving both lean percentage of the carcass and backfat thickness.

• The Journal of Korean Physical Therapy
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• 제17권4호
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• pp.537-557
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• 2005

The purpose of this study was to investigate the effect of pelvic traction and muscle energy technique(MET) for hemiplegic patients on static standing balance. The scale for static standing balance is measured by using mean balance(%), frequence(Hz), sway area($mm^2$), sway path(mm), max sway velocity(mm/s), ant/post sway angle($^{\circ}$), lateral sway angle($^{\circ}$), sway number, change of pelvic height is measured in relation to the height of ASIS and PSIS. The subjects of this study were thirty hemiplegic patients: 15men and 15 women, with an average ages of 50.80 years. The thirty subjects were divided into 3 group of 10 at random ; ten subjects had pelvic traction after bobath therapy (the pelvic traction group), ten subjects had muscle energy technique after bobath therapy (the MET group), and ten subjects had only bobath therapy (the control group). Static standing balance was measured using BPM (balance performance monitor; data print software version 5.3), pelvic height was measured using height measuring with an adjustable horizontal arm. In order to assure the statistical significant of the result, an one-way ANOVA, the paired t-test, and a person's correlation were applied at the.05 level of significance. The results of this study were as follows: 1) The change in pelvic height were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). 2) The change in affected and non-affected on weight bearing were not statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p>.05). 3) The change in frequency were statistically significant between the MET group, the control group and the pelvic traction group on pre-treatment and post-treatment(p<.05). 4) The change in sway area were statistically significant between the MET group, the control group and the pelvic traction group on pre-treatment and post-treatment(p<.05). 5) The change in sway pa1h were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). 6) The change in max sway velocity were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). 7) The change in ant/post sway angle were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). 8) The change in lateral sway angle were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). 9) The change in sway number were statistically significant between the pelvic traction group, the MET group and the control group on pre-treatment and post-treatment(p<.05). In conclusion, there was a difference between the experimental group and the control group. In the future, we have to study continuously about pelvic traction and muscle energy technique in hemiplegic patients.

• Radiation Oncology Journal
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• 제28권2호
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• pp.57-63
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• 2010

목 적: 방사선 치료를 받은 코인두암 환자의 치료 전 조직을 면역조직화학염색하여 생체분자적 예후인자를 찾고자 하였다. 대상 및 방법: 1998년부터 2006년까지 방사선 치료를 받은 코인두암환자는 68명이었다. 이중 38명의 환자에서 면역조직화학염색을 위한 파라핀 블록을 찾을 수 있었다. 전체 환자 중 31명은 미분화암종이었고, 7명은 편평세포암종이었다. 전체 환자의 84%가 2002 American Joint Committee on Cancer Stage III or IV 환자였다. 전체 환자의 파라핀 블록을 이용하여 Met, COX-2, epidermal growth factor receptor (EGFR), nm23-H1에 대해 면역조직화학염색을 시행하였다. 결 과: 전체 환자의 중앙 추적 기간은 30개월이었고 생존 환자의 중앙 추적 기간은 39개월이었다. 높은(${\geq}50%$) Met 발현을 보인 환자들의 5년 생존율은 48%, 낮은 Met 발현을 보인 환자들의 5년 생존율은 84%로 이는 통계적으로 유의한 차이를 보였다(p=0.02). Met 발현 정도는 다변량 분석에서도 유의한 인자로 분석되었다(p=0.01). Met 발현은 종양의 병기, 성별, 나이, 항암제나 방사선 치료에 대한 반응 정도와 상관관계를 보이지 않았다. Met 발현 정도는 COX-2 발현과 중등도의 상관관계를 보였으나(Pearson coefficient 0.496, p<0.01), COX-2 발현은 전체생존율에 영향을 미치지 않았다. 본 연구에서는 nm23-H1이나 EGFR의 발현은 예후인자가 아닌 것으로 분석되었다. 결 론: 방사선치료를 받은 코인두암에서, 높은(${\geq}\;50%$) Met 발현은 전체생존율에 영향을 미치는 독립적인 예후인자일 가능성이 있다.

• 한국보건간호학회지
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• 제31권2호
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• pp.376-388
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• 2017

Purpose: The triglyceride-to-high-density lipoprotein-cholesterol (TG/HDL-C) ratio is one of the main predictive indices for cardiovascular disease. This study was examined the relationship between TG/HDL-C ratio and metabolic syndrome (MetS) in male office workers. Methods: Secondary analysis was conducted to determine the risk between the TG/HDL-C ratio and MetS in male office workers. A total of 765 people underwent the 'regular workplace health checkups in 2014'. Among the subjects who were male and responded to the questionnaire and health lifestyle survey, 470 (61.4%) excluding those with missing and/or abnormal values were analyzed. The association between MetS, MetS components, and the TG/HDL-C ratio was examined by a Chi-square test, One-way ANOVA, Turkey post-hoc test and Logistic regression analysis. Results: The number of males with MetS was 70 (14.9%) and the number of MetS components increased with increasing TG/HDL-C ratio (p<.001). Logistic regression analysis with an adjustment for potential confounders revealed a 31.8 times higher odds ratio of the Quartile4 group for MetS than that of the Quartile1 group (p<.001). Conclusion: These results show that the likelihood of MetS, particularly the risk of MetS in the Quartile4, increases with increasing TG/HDL-C ratio.

• 대한두경부종양학회지
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• 제27권1호
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• pp.3-11
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• 2011

Hepatocyte growth factor(HGF) and c-Met play an important role in the control of tumor growth and invasion, and they are known to be good prognostic indicators of patient outcome. Epigallocatechin-3-gallate (EGCG) has been shown to have chemopreventive and therapeutic properties by modulating multiple signal pathways regarding the control of proliferation and invasion of cells. In this study, we evaluated the role of c-Met in EGCG-induced inhibition of invasion and apoptosis in an oral cancer cell line. In KB cells where c-Met was knocked down with siRNA, we performed invasion assay and FACS with Annexin V-FITC/PT staining. In addition, we checked the change of mitochondrial membrane potential(MMP) and the generation of reactive oxygen species(ROS). EGCG-induced inhibition of invasiveness was significantly decreased after the knock-down of c-Met. EGCG-induced apoptosis, MMP change and ROS generation was also reduced in c-Met knock-ed-down KB cells. These results suggest that c-Met is involved in EGCG-induced apoptosis and inhibition of invasiveness of oral cancer cell line.