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Analysis of (-)-Epigallocatechin-3-Gallate-Induced Apoptosis and Inhibition of Invasiveness in Oral Cavity Carcinoma Squamous Cell Carcinoma According to Expression of c-Met  

Shin, Yoo-Seob (Department of Otolaryngology, Ajou University School of Medicine)
Koh, Yoon-Woo (Department of Otolaryngology, Yonsei University College of Medicine)
Choi, Eun-Chang (Department of Otolaryngology, Yonsei University College of Medicine)
Kang, Sung-Un (Department of Otolaryngology, Ajou University School of Medicine)
Hwang, Hye-Sook (Department of Otolaryngology, Ajou University School of Medicine)
Choo, Oak-Sung (Department of Otolaryngology, Ajou University School of Medicine)
Lee, Han-Bin (Department of Otolaryngology, Ajou University School of Medicine)
Kim, Chul-Ho (Department of Otolaryngology, Ajou University School of Medicine)
Publication Information
Korean Journal of Head & Neck Oncology / v.27, no.1, 2011 , pp. 3-11 More about this Journal
Abstract
Hepatocyte growth factor(HGF) and c-Met play an important role in the control of tumor growth and invasion, and they are known to be good prognostic indicators of patient outcome. Epigallocatechin-3-gallate (EGCG) has been shown to have chemopreventive and therapeutic properties by modulating multiple signal pathways regarding the control of proliferation and invasion of cells. In this study, we evaluated the role of c-Met in EGCG-induced inhibition of invasion and apoptosis in an oral cancer cell line. In KB cells where c-Met was knocked down with siRNA, we performed invasion assay and FACS with Annexin V-FITC/PT staining. In addition, we checked the change of mitochondrial membrane potential(MMP) and the generation of reactive oxygen species(ROS). EGCG-induced inhibition of invasiveness was significantly decreased after the knock-down of c-Met. EGCG-induced apoptosis, MMP change and ROS generation was also reduced in c-Met knock-ed-down KB cells. These results suggest that c-Met is involved in EGCG-induced apoptosis and inhibition of invasiveness of oral cancer cell line.
Keywords
HGF(Hepatocyte Growth Factor); c-Met; EGCG; Oral cavity cancer; Apoptosis; Invasion;
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