• Title/Summary/Keyword: MCHC

Search Result 168, Processing Time 0.024 seconds

The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value in vitro (시험관내에서 captafol이 혈액학, 적혈구막 및 혈장생화학치에 미치는 영향)

  • Kim, Sung-hoon;Hwang, Hwa-seon;Cha, Shin-woo;Han, Sang-seop;Roh, Jung-koo
    • Korean Journal of Veterinary Research
    • /
    • v.33 no.3
    • /
    • pp.507-511
    • /
    • 1993
  • The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value was investigated using blood of SPF Sprague-Dawley male rats in vitro. For the anticoagulations, we used 0.5mg of heparin per $10m{\ell}$ of blood from vena cava. Three con-centrations($0.1{\times}10^{-4}M$, $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$) captafol in ethanol were added to the each $2.5m{\ell}$ blood so that the linal concentration of ethanol was 1%. The blood contained with each concentration of captafol was incubated at $37^{\circ}C$ C for 2 hours under 5% $CO_2$ gas The whole blood and plasma were examined for hematological vaJues, erythrocyte membrane damage and biochemical values, respectively. The results obtained were summarized as follows ; 1. The number of RBC in $1{\times}10^{-2}M$ group and the concentration of MCHC in $1{\times}10^{-3}M$ group were significantly (p<0.05) decreased and increased from that of control values, respectively. The percentage of Hct was significantly (p<0.05) decreased with dose-response. 2. Erythrocyte fragility rate of $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ group were significantly (p<0.01) increased from that control with dose response. 3. Potassium ion level of $1{\times}10^{-2}M$ was significantly (p<0.05) increased from that of control. 4. The concentration of total bilirubin in the $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ groups were significantly increased from that of control. The enzyme level of creatine kinase in $1{\times}10^{-2}M$ group was significanlty (p<0.05) decreased from control value.

  • PDF

Effects of Ad libitum and Restricted Feeding of Concentrates on Body Weight Gain, Feed Intake and Blood Metabolites of Hanwoo Steers at Various Growth Stages (배합사료의 자유 및 제한 급여가 거세한우의 성장단계별 증체, 사료섭취량 및 혈중 대사물질에 미치는 영향)

  • Kwon, E.G.;Hong, S.K.;Seong, H.H.;Yun, S.G.;Park, B.K.;Cho, Y.M.;Cho, W.M.;Chang, S.S.;Shin, K.J.;Paek, B.H.
    • Journal of Animal Science and Technology
    • /
    • v.47 no.5
    • /
    • pp.745-758
    • /
    • 2005
  • Two hundred fifty eight Hanwoo steers were used in a completely randomized design experiment to determine the effects of ad libitum or restricted feeding of concentrates on body weight(BW) gain, feed intake, blood metabolites and hematological parameters. Steers were assigned at 6 months of age to feeding groups of ad libitum(T1) or restricted(T2) by 18 months of age. Steers in both groups were fed ad libitum from 19 months of age. The restrictive feeding levels were 1.2-1.5% of BW for the growing period and 1.7-1.8% of BW for the early fattening period. Average daily gains were significantly higher in T1 than in T2 from 10 to 14 months of age, but were significantly higher in T2 than in T1 from 20 to 24 months of age(p<0.05). Total dry matter intake(DMI) was higher in T1 than in T2 at 10, 12 and 16 months of age(p<0.05). Total DMI of T2 was higher than that of T1 at 22 months of age(p<0.05). Feed conversions were significantly lower in T2 than in T1 from 20 to 30 months of age(p<0.05). Blood albumin concentrations were significantly higher in T2 than in T1 at 12, 14, 16 and 18 months of age. Blood triglyceride concentrations were significantly higher in T1 than in T2 at 14 and 16 months of age(p<0.05). Blood inorganic phosphorus concentrations were significantly higher in T2 compared with T1 at 8, 10, 16 and 22 months of age(p<0.05). Mean corpuscular volume and mean corpuscular hemoglobin were significantly lower in T2 than in T1 from 8 to 12 months of age(p<0.05), but those were significantly higher in T2 than T1 from 10 months to 12 months of age(p<0.05). Present results may indicate that the restricted feeding for the growing period does not show adverse effects on body weight gain with better feed conversion for the following late fattening period.

Hematological Characterietic of Bastard halibut according to Cultured experiment of Mari-floating netcage- I On the Hematological conditions (넙치의 해상(海上)가두리 사육시험(飼育試驗)에 따른 혈액학적(血液學的) 연구(硏究)-I 혈액성상(血液性狀)에 대(對)하여)

  • Sim, Doo-Saing;Jeon, Im-Gi;Jung, Sung-Hee
    • Journal of fish pathology
    • /
    • v.3 no.1
    • /
    • pp.27-38
    • /
    • 1990
  • Hematological conditions were measured to the cultured bastard halibut classified by control group and establishments of mari-floating netcages from April 7, 1990 to May 24, 1990. The RBC, Hb, HT, MCHC, MCH and MCV values were $289{\pm}61{\times}10/^4/mm^3$, $4.8{\pm}0.9g/dl$, $22{\pm}6%$, $22.7{\pm}4.0%$, $16.9{\pm}3.3pg$ and $77{\pm}19{\mu}m^3$ respectively in the hematological conditions of control group. Relation between Hb and Ht was represented highly positive(r=0.805, p<0.01>, and relation between MCV and MCH was represented positive (r=0.696, p<.0.05) in the control group. In the variation of hematological conditions classified by experimental group, RBC of PVC group was returned $293{\pm}57{\times}10/mm^3$ on the 21th after locomotion to mari-floating netcages. RBC of netron group was also returned as control level on the 21th after locomotion, showed stabilization. Hb of PVC and netron group was low in the range of variation, was represented $3.9{\pm}9.6g/dl$ on the 45th after locomotion. The group that was also satabilizied variation of Ht were PVC, netron group. According to the results of hematological conditions classified by establishment of mari-floating netcages, the experiment group that were stabilized variation were in order of PVC, netron and pole-PVC group. But the other groups were extended the range of variation.

  • PDF

Effects of Light Sources in Poultry House on Growth Performance, Carcass Yield, Meat Quality and Blood Components of Finishing Broilers (계사 내 광원이 육계 후기의 생산성, 도체수율, 육질 특성 및 혈액성분에 미치는 영향)

  • Hong, Eui-Chul;Kang, Bo-Seok;Kang, Hwan-Ku;Jeon, Jin-Joo;You, Are-Sun;Kim, Hyun-Soo;Son, Jiseon;Kim, Chan-Ho;Kim, Hee-Jin
    • Korean Journal of Poultry Science
    • /
    • v.47 no.3
    • /
    • pp.159-167
    • /
    • 2020
  • This study investigated the effect of different light sources in the poultry house on performance, meat quality, and blood composition of finishing broilers. Two hundred and forty male broilers (1-day-old, 42.2±0.1 g) were divided into three groups and subjected to different light source treatments (incandescent, LED, and fluorescent lamps) from 3 weeks of age (four replications/treatment, 20 birds/replication). After breeding for 6 weeks, the carcass yield and meat quality of broilers with similar body weight (BW; 3.4±0.07 kg) were investigated, and blood components were analyzed. Corn-soybean meal-based feed was provided as starter (CP 22.5%, ME 3,020 kcal/kg), early (CP 18.5%, ME 3,050 kcal/kg), and finishing (CP 18%, ME 3,100 kcal/kg). Performance, carcass yield, meat quality, and blood components were evaluated. BW, BW gain, feed intake, and feed conversion ratio did not show any significant differences among treatments. There was no significant difference on live weight and carcass yield among treatments. There was no significant difference on meat color, shear force, and water holding capacity; however, cooking loss at 17.2% was the highest in the LED treatment (P<0.05). There was no significant difference on blood components except for glucose (blood biochemistry component) among treatments. Glucose was 234.5 mg/dL, 256.9 mg/dL, and 250.1 mg/dL in the three treatments, respectively, with a significant difference between incandescent and LED treatments (P<0.05). These results are used useful as basic data for investigating the effect of lighting in broilers production.

Relation Between Nutritional Factors and Bone Status by Broadband Ultrasound Attenuation among College Students (대학생의 골초음파 상태에 영향을 미치는 영양요인 분석)

  • Kwon, Se-Mi;Lee, Byung-Kook;Kim, Hee-Seon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.11
    • /
    • pp.1551-1558
    • /
    • 2009
  • The management of skeletal health in the twenties is the number one priority for preventing fracture or osteoporosis occurrence in later life cycle. Therefore, the factors influencing bone mineral density were examined by anthropometric measurements, food intakes, bone mineral density and biochemical nutritional indexes in blood among college students. Among the students who received health examinations from May to December, 2007, 532 male and 507 female students who agreed to participate in the study were selected. Nutritional intakes were estimated with 3-day 24-hour recall method. Bone mineral density was measured by ultrasonic attenuation passing through right calcaneus bone and expressed as broadband ultrasound attenuation and t-score was calculated by WHO criteria. Red blood cell count, hemoglobin, hematocrit values were measured by whole blood analysis, and alkaline phosphatase, serum calcium and serum iron were used as indexes for biochemical nutritional status. Data analysis was conducted using SPSS 14.0 program, and protecting and risk factors on bone health status were analyzed by logistic regression analysis between normal bone health group (t-score$\underline{\geq}$ -1.0) and osteopenia group (t-score<-1.0). The results showed that more people belong to the normal bone health group probably because this study was conducted among those with their peak bone density. Biochemical nutritional status and nutrition intakes of both groups for normal and osteopenia did not show statistically significant difference except MCHC, animal protein and animal iron intakes in female. According to the results of logistic regression analyses, dietary intakes of animal protein, animal iron and zinc showed protecting effects against osteopenia. Therefore, dietary nutritional intakes of micronutrients, especially iron and zinc are important for bone health of young people.

Relation Between Obesity Indices and, Nutritional Knowledge Nutritional Status and Blood Parameters in Obese Middle-School Students (비만 중학생의 비만도와 영양지식, 영양섭취상태 및 혈액성상과의 상관성)

  • Choi, Mi-Kyeong;Kim, Mi-Hyun;Lee, Yoon-Shin;Cho, Hye-Kyung;Kim, Kyeong-Hi;Lee, Bo-Bae;Sung, Mi-Kyung;Sung, Chung-Ja
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.34 no.2
    • /
    • pp.181-189
    • /
    • 2005
  • The purpose of this study was to investigate the relationship between obesity indices, nutritional knowledge, nutritional intake status, and some blood parameters in obese middle-school students. Anthropometric measurement, 24-hour recall for dietary intake, blood analysis and Questionnaire response including nutritional knowledge, nutritional attitude, and self-satisfaction were conducted in 42 obese middle-school students (28 male and 14 female) participated in the nutritional camp program carried by Bucheon district public health center in Kyunggi-do. The mean age of subjects was 13.9 years. The average body weight, BMI, obesity index, WHR and percent body fat were 75.5 kg, 29.1 kg/$m^2$, 30.1, 0.89 and 33.3% in male subjects, and 67.8 kg, 27.5 kg/$m^2$, 25.3, 0.81 and 34.6% in female subjects. The average nutritional attitude, the self-satisfaction and the nutritional knowledge scores were no significant difference between male and female subjects. Average intake of energy were 85.5% of RDA in males and 98.1% of RDA in females. The percentage of energy from fat was higher in both male (29%) and female (26%) subjects than recommended level. Calcium, iron, zinc, vitamin A, vitamin B$_2$, vitamin C, niacin and folate intakes were below the RDA for them. In the results of correlation analysis, body weight, BMI and obesity index were positively correlated with systolic blood pressure and cholesterol intake. There was a negative correlation between percent body fat and nutritional knowledge. Body weight was negatively related to HDL-cholesterol, whereas it has positive relation with RBC, hemoglobin and hematocrit. BMI and obesity index were negatively related to HDL-cholesterol, but positively related to RBC, respectively. WHR showed positive relationship with serum GPT, glucose and MCV.

Analysis of Growth and Hematologic Characteristics of Endangered Korean Native Cattle (멸종위기의 백한우(체세포복제 포함)의 성장 및 혈액학적 특성 분석)

  • Kim, Hyun;Choe, Changyong;Seong, Hwan-Hoo
    • Journal of Embryo Transfer
    • /
    • v.30 no.3
    • /
    • pp.175-181
    • /
    • 2015
  • The objective of this study was to monitor health conditions of genetically identical somatic cells cloned Korean white cattle, endangered indigenous cattle (EIC) and indigenous cattle (IC) by analysis of hematologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and one live cattle were delivered by caesarean section. The cloned Korean white cattle were genetically identical to the nuclear donor cattle. As a result, the mean values of RBC and platelet of cloned cattle and white cattle were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between cloned cattle and IC of the same age (1~2 years) showed the statistical significance (P<0.05). Also, in the WBC of Korean white cattle, the estimated values were decreased according to the age from $12.0{\times}10^3/{\mu}l$ under 1 year to $11.0{\times}10^3/{\mu}l$ over 1 years respectively. Although clone-cattle had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned cattle were not different when compared to reference range. This study suggests that cloned Korean white cattle derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological parameters. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

Studies on the Meat Production and Woolskin Processing of Sheep and Korean Native Goats for Increasing Farm Income as a Family Subsidiary Work (농가부업(農家副業)의 소득향상(所得向上)을 위한 양육생산(羊肉生産) 및 모피가공(毛皮加工)에 관(關)한 연구(硏究))

  • Kwon, Soon-Ki;Kim, Jong-Woo;Han, Sung-Wook;Lee, Kyu Seung
    • Korean Journal of Agricultural Science
    • /
    • v.5 no.2
    • /
    • pp.93-114
    • /
    • 1978
  • The purpose of the study was to find out possible ways for increasing farm income through the sheep and Korean native goats farming, and to investigate meat productivity, wool productivity; woolskin utility, physiological characteristics and correlation between economical college animal farm of the Chungnam National University and sample farms in the suburbs of Dae jeon City were selected for feeding 20 heads of Corriedale wethers and another 20 heads Korean native kids as research materials for the periods of 5th May-26th November, 1977. The data such as growth rate, carcass, viscera weight, blood picture and plamsa components, hebage intake and economic traits were obtained and analysed. The result of the study are summarized as follows: 1. Meat production and quality 1) After 196days of feeding, the body weight of sheep and Korean native goats was increased by two times of those at the beginning of the trial, i.e. 20kg and 8kg respectively. 2) There was no significance of growth rates of sheep in housing and grazing. 3) The growth rate of Korean native goats were excellent at the mountainous areas of Gong ju-Gun where infectious diseases were not found 4) Accroding to the body measurements of 18-month-old sheep, percentages of hip height, body length, rump length, chest depth, chest width, hip width, chest girth and forearm circumference to the withers height were 103,%, 104%, 33%, 44%, 31%, 23%, 135% and 15% respectively, and those of hip height, body length, chest depth and chest girth of 8-month-old native goats to the withers height were 106%, 109%, 46% and 122,% respecitively. As a result, it was found that the percentage of hip height, body length and chest depth of Korean native goats were higher than those of sheep while that of the chest girth of goats was lower. 5) In the carcass data, 47, $52{\pm}2.27%$ of carcass percentage, $34.61{\pm}1.62%$ of lean meat, $26.07{\pm}2.51%$ of viscera, $9.75{\pm}1.4%$ of bone, and $20.95%{\pm}2.14%$ of woolskin for sheep, and $45.58{\pm}5.63%$ of carcass percentage, $27.62{\p}3.81%$ of meat, $34.86{\pm}4.16%$ of viscera, $11.66{\pm}1.83%$ of bone, $3.63{\pm}1.61%$ of skull and $9.26{\pm}2.41%$ of woolskin for native goats were obtained. 6) The contents of moisture, crude protein, crude fat and crude ash in native goat meat were much similar in both plots of housing and grazing. It was, however, known that the contents of moisture and protein were higher in grazinrg than in housing, while fat content was lower in grazing plots. 7) The weights of visceral organs shown similar tendency for both of sheep and native goats. For the weights of liver, heart, kidney and spleen, significance was not reconized among the treatments. Those of rumen, reticulum, small and large intestine were heavier in grazing than in housing, while the amount of visceral fat was heavier in housing. 2. Wool productivity and woolskin 1) The wool production of sheep for 7 months was $3.88{\pm}1.02kg$, and wool percentage, staple length, straighten length, wool growth per day and number of crimps were $9.27{\pm}1.48%$, 8. $47{\pm}1.00cm$, $10.63{\pm}0.99cm$, $0.40{\pm}0.04cm$ and $2.78{\pm}0.40$ respecitively. 2) The tensile strength and tear strength of woolskin treated by alum tanning were highest on the skin obtained from rump, i.e. $1,351kg/mm^2$ and $2,252kg/mm^2$ respectively, and they are in order of loin and shoulder. 3. Utilization and improvement of pasture. 1) The difference of herbage intake of native goats was not recognized between grazing and tethering, but the intake in the afternoon was s lightly higher than that in the morning. However the hervage intake of sheep was superior in grazing and in the afternoon. 2) The cultivation effect was lower in the native goat plots due to their cultivation abilities, in other words, the establishment rates of pasture by hoof cultivation were 60.25% in the goat plots and 77.35% in the sheep plots. 4. Correlation among economical traits. 1) The correlation between live weight of sheep and daily gain was higher. On the other hand, the correlation between other traits was not significant except that live weight, daily gain and lean meat percentage to the length of thoracic vertebrae. The live weight of native goats and meat production were highly correlated, and high correlation was also found between weights of carcass and meat. However, negative correlation was shown between viscera weight and live weight as well as daily gain. 2) The correlatoin between fleece weight of sheep and other traits such as live weight, daily gain and fleece percentage is very high at the 1% siginficant level, and this means that rapid-growth individuals can produce much fleece. 3) The correlation between the factors such as weights of live body, lean meat and viscera of sheep and body measurements, i. e. chest girth and body length was highest, and weights, of carcass and lean meat was highly correlated to chest width and depth. It will be therefore reasonable that the meat productivity estimates will have to be made on the basis of chest girth and body length. The meat production traits of native goats were highly correlated to the most of body measurement data, and the correlation coefficient between chest girth and weights of live body, carcass, lean meat and bone percentage was very high, i. e. 0.992-0.974 in particular. The correlations of meat production traits to chest depth, forearm circumference, body length were 0.759-0.911, 0.759-0.909 and 0.708-0.872 respectively. Therefore, the meat production of native goats will have to be estimated on the basis of chest data. 5. Blood picture and plasma components. 1) The number of erythrocyte and MCHC of native goats were $12.93{\times}10^6/mm^3$ and 36.14%, and those of sheep were $10.68{\times}10^6/mm^3$ and 36.26 respectively. The values of native goats were significantly higher than those of sheep. 2) The hemoglobin concentration, PVC, MCV and MCR of native goats were 10.92 g/100ml, $23.40{\mu}^3$ and 10.94 pg, and those of sheep were 11.73 g/100ml, 36.25 ml/100ml, $33.97{\mu}^3$ and 30.2 ml/100ml 8.43 pg respectively. The values of native goats were significantly lower those of sheep. 3) The number of leukocytes of native goats was significantly higher than that of sheep, that is, $11.64{\times}10^3/mm^3$ in native goats and $9.32{\times}10^3/mm^3$ in sheep. 4) In differential count of leukocyte, neutrophil was significantly high in native goats while lympocyte in sheep. On the other hand, the basophil, eosinophil and monocyte were not significant between native goats and sheep. 5) The amounts of total protein and glucose in the plasma of native goats were 6.2g/100ml and 53.6mg/100ml, and those of sheep were 5.6g/100ml and 45.7mg/100ml, which means that the values of native goats were significantly higher that those of sheep. The amount of total-lipid of native goats(127.6mg/100ml) was significantly than that of sheep(149.6mg/100ml). 6) The amount of non-protein nitrogen, cholesterol, Ca, P, K, Na and Cl were not different between native goats and sheep. 6. Economic analysis. 1) The gross revenue of a farm which fed native goats and sheep was 4,000won per head and the optimum size for feeding them in a farm as a subsidiary work is 5-10 heads. 2) Since there was no difference between housing and grazing, they can be fed in group for farm's subsidiary work. 3) They can be also fed by youths and house wives in the suburbs of cities, because labour requirement is estimated as only two hours per days for feeding 5 heads of native goats and sheep.

  • PDF