Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Analysis of Growth and Hematologic Characteristics of Endangered Korean Native Cattle

멸종위기의 백한우(체세포복제 포함)의 성장 및 혈액학적 특성 분석

  • Kim, Hyun (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Choe, Changyong (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김현 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2015.01.30
  • Accepted : 2015.09.04
  • Published : 2015.09.30
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Abstract

The objective of this study was to monitor health conditions of genetically identical somatic cells cloned Korean white cattle, endangered indigenous cattle (EIC) and indigenous cattle (IC) by analysis of hematologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and one live cattle were delivered by caesarean section. The cloned Korean white cattle were genetically identical to the nuclear donor cattle. As a result, the mean values of RBC and platelet of cloned cattle and white cattle were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between cloned cattle and IC of the same age (1~2 years) showed the statistical significance (P<0.05). Also, in the WBC of Korean white cattle, the estimated values were decreased according to the age from $12.0{\times}10^3/{\mu}l$ under 1 year to $11.0{\times}10^3/{\mu}l$ over 1 years respectively. Although clone-cattle had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned cattle were not different when compared to reference range. This study suggests that cloned Korean white cattle derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological parameters. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

본 연구에 사용된 공시축은 2013년 1월부터 2014년 12월까지 국립축산과학원 가축유전자원센터에서 사육 중인 모색을 기준으로 동일연령인 일반한우(황우), 백한우 및 복제소 18두(황우 10두, 백우 7두 그리고 복제소 1두)를 대상으로 혈액학적 수치를 확인하였다. 동일연령의 복제소와 백한우의 성장 단계별 혈액학적 검사 결과에서 1년 이하에서 1~2년으로 연령이 증가됨에 따라 RBC 관련 6개의 인자의 측정수치가 정상치 범위내 임을 확인했다. 또한 일반한우와의 비교에서도 차이점이 인정되지 않았다. 하지만, 복제소를 포함하는 백한우군와 일반 한우군 간의 RBC($11.2{\sim}10.1{\times}10^6/{\mu}l$, $12.0{\sim}10.3{\times}10^6/{\mu}l$로)와 PL치($592{\sim}448{\times}10^3/{\mu}l$, $589{\sim}450{\times}10^3/{\mu}l$로)에서 각각 유의성 있는 감소(P<0.05)가 인정되었다. 또한, 복제소와 백한우의 백혈구계 검사 결과에서도 1년 이하에서1~2 년으로 연령이 증가함에 따라 WBC 수치($12.0{\sim}11.1{\times}10^3/{\mu}l$, $12.5{\sim}10.0{\times}10^3/{\mu}l$)가 감소했지만, 정상치 범위 내의 결과를 확인했다. 백혈구 분포의 백분율에서는 전반적으로 복제소 및 백한우의 림프구가 41.0~39.0%, 43.1~41.6%이며, 분엽형 호중구 수치는 32.0~29.0%, 33.9~30.9%로 나타났다. 일반 한우군과 복제소를 포함하는 백한우군 간의 백혈구계의 림프구 수치의 비교에서는 백한우군(41.6%)로, 일반한우(45.1%)에 비하여 유의적으로 낮음(P<0.05)을 확인하였다. 본 연구결과를 통하여 백한우의 귀체세포 유래의 복제소의 혈액학적 분석 결과로 건강상에 문제점이 없음을 확인하였다.

Keywords

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