• Journal of Animal Science and Technology
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• 제46권6호
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• pp.897-902
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• 2004

이전에 연구된 한우육과 Holstein 및 Black angus육 감별법의 신속성, 편리성, 경제성 등의 단점을 보완하기 위해 소의 MC1R gene의 SNP를 이용한 새로운 감별법을 시도하였다. 본 연구에서는 소의 MC1R gene 중 594번째 염기인 Guanine이 한우에서는 결실된 점을 이용하여 한우의 sequence를 바탕으로 3 쪽에 2mers의 tail을 달아 한우에게는 상보적이나 다른 종에서는 상보적이지 않은 3 -tailed primers를 제작하였다. 이 primer들을 이용해서 한우에서만 MC1R 중 571번째 염기서열부터 919번째 염기서열까지의 343bp의 단편이 증폭되도록 하였다. 그 결과, Holstein, Black angus에서는 모두 band가 관찰되지 않았으나 한우에서는 343bp의 band가 확인되었다. 따라서 본 연구에서 사용한 3 -tailed primer를 이용하면 보다 정확하고 재현성 있으며 신속하고 편리하며 경제적인 한우육의 감별이 될 것으로 판단된다.

• 동의생리병리학회지
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• 제17권3호
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• pp.643-647
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• 2003

The Methylene Chloride(MC) fraction of Trichosanthis kirilowii Maxim has been investigated anti-tumor activities in vitro. The MC fraction of Trichosanthis kirilowii Maxim significantly inhibited the proliferation of human leukemic U937 cell with an IC50 of approximately 10μg/ml in a dose-dependent manner. We found that the MC fraction upregulated of caspase9 and caspase-3 activity and cleaved PARP expression but it didn't affect bax and bcl-2. which were demonstrated by western blot analysis. Taken together, these results exerted that the MC fraction suppessed human leukemic U937 cell proliferation by inducing apoptosis, suggesting the MC fraction of Trichosanthis kirilowii Maxim is possible to show anti-cancer activity in vivo.

• The Journal of Advanced Prosthodontics
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• 제8권3호
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• pp.235-240
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• 2016

PURPOSE. In this study, the aim of this study was to evaluate the effect of implant surface treatment on cell differentiation of osteoblast cells. For this purpose, three surfaces were compared: (1) a modified SLA (MSLA: sand-blasted with large grit, acid-etched, and immersed in 0.9% NaCl), (2) a laser treatment (LT: laser treatment) titanium surface and (3) a laser and acid-treated (LAT: laser treatment, acid-etched) titanium surface. MATERIALS AND METHODS. The MSLA surfaces were considered as the control group, and LT and LAT surfaces as test groups. Alkaline phosphatase expression (ALP) was used to quantify osteoblastic differentiation of MC3T3-E1 cell. Surface roughness was evaluated by a contact profilometer (URFPAK-SV; Mitutoyo, Kawasaki, Japan) and characterized by two parameters: mean roughness (Ra) and maximum peak-to-valley height (Rt). RESULTS. Scanning electron microscope revealed that MSLA (control group) surface was not as rough as LT, LAT surface (test groups). Alkaline phosphatase expression, the measure of osteoblastic differentiation, and total ALP expression by surface-adherent cells were found to be highest at 21 days for all three surfaces tested (P<.05). Furthermore, ALP expression levels of MSLA and LAT surfaces were significantly higher than expression levels of LT surface-adherent cells at 7, 14, and 21 days, respectively (P<.05). However, ALP expression levels between MSLA and LAT surface were equal at 7, 14, and 21 days (P>.05). CONCLUSION. This study suggested that MSLA and LAT surfaces exhibited more favorable environment for osteoblast differentiation when compared with LT surface, the results that are important for implant surface modification studies.

• Imaging Science in Dentistry
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• 제33권1호
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• pp.51-57
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• 2003

Purpose: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-El osteoblastic cell line, particularly an the expression of type I collagen and alkaline phosphatase mRNA. Materials and Methods: Cells were irradiated with a single dose of 0.5, 1, 2, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. The specimens were then harvested and RNA extraction was carried out at 1 and 3 days after irradiation. The extracted RNA strands were reverse-transcribed and the resulting cDNA fragments were amplified by PCR. Results: The irradiated cells demonstrated a dose-dependent increase in type I collagen mRNA expression relative to the control group, with a maximum level of type I collagen mRNA expression occurring at 8 Gy. The degree of type I collagen mRNA expression increased significantly at 1 day after irradiation, but little differences were found between the control group and at the 3rd day. The amount of alkaline phosphatase mRNA expression increased significantly at land 3 days after irradiation in the 1 Gy exposed group compared with the control group. Conclusion: The amount of type I collagen and alkaline phosphatase mRNA expression increased significantly 1 day after irradiation when compared with the control group.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2003년도 The Korea-Japan Joint Symposium
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• pp.41-42
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• 2003

Osteoblasts (MC3T3-E1) were cultured on polysaccharide type polyelectrolyte complex (PEC). The growth of the MC3T3-E1 on the PEC with carbxyl group (c-type) was slightly suppressed and exhibited aggregation morphology. On the other hand, cell growth on the PEC with sulfate group (s-type) was enhanced and the cell exhibited spreading form. Differentiation markers of osteoblast (ALPase activity, calcification, expression of osteocalsin) were enhanced and localized around cell aggregates on c-type PECs. These results suggest that PEC has the ability to control osteoblast proliferation and differentiation.

• Journal of Periodontal and Implant Science
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• 제31권1호
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• pp.123-135
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• 2001

Several growth factors and polypeptides were studied for the regeneration of periodontal supporting tissues which had been lost due to periodontal disease. But these are not commonly used for regenerators of bone tissue or alveolar bone, because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many natural products, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential or periodontal tissues. Cnidii Rhizoma, Rhinocerotis Cornu and Drynariae Rhizoma have been traditionally used as a drug for treatment of bone disease in oriental medicine. The purpose of this study was to examine the ability of alkaline phosphatase synthesis of MC3T3-E1 cells when above medicines were supplimented. MC3T3-E1 cells were cultured with ${\alpha}-MEM(negative control)$, dexamethasone(positive control), and each natural products for 3 and 5 days. And then ALP synthesis was measured by spectrophotometer for enzyme activity and by naphthol AS-BI staining for morphometry. Except Cnidii Rhizoma, all of the natural products of this study induced higher activity of ALP synthesis than controls. Among them Drynariae Rhizoma induced the highest activity. In the aspects of culturing time, all medicines did not showed the difference between 3 and 5 days, but $10^{-7}g/ml$ group of Rhinocerotis Corun showed significant increase at 3 days than at 5 days. These results indicate that several natural products have a inducing ability of ALP synthesis on osteoblasts.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.347-360
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• 2000

Bone remodeling results from the combined process of bone resorption and new bone formation which is regulated in part by some of the polypeptide growth factors such as platelet derived growth factor(PDGF), which has been known to be an important local regulator of bone cell activity and participate in normal bone remodeling. This process includes strictly regulated gene expression of several bone matrix proteins such as type I collagen and osteopontin, a 44 kDa phosphorylated glycoprotein, which has important roles in bone formation. The purpose of this study is to evaluate the effecs of PDGF-BB on the mRNA expression of bone matrix protein, type I collagen and osteopontin, in MC3T3- E1 cell culture. Cells were seeded at $5{\times}10^5$ cells in 10 ml of minimum essential medium alpha(${\alpha}-MEM$) containig 10% fetal bovine serum, 10 mM beta glycerophosphate. 0.1, 1, 10 ng/ml PDGF-BB were added to the cells for the day 3, 7, 14, 21, 28 and cultured for 24 hours. Type I collagen cDNA, Hf677, and osteopontin cDNA were used as probes for northern blot analysis. Total cellular RNA was purified at indicated day and northern blot analysis was performed. The results were as follows : Type I collagen mRNA expressions were higher at the day 3 and 7, and lower in the day 14, 21 in the control groups. In the experimental groups, mRNA expressions were increased when 0.1 ng/ml PDGF-BB were added on the day 3, 7, 21, and decreased in dose-dependent manner on the day 14, decreased at all added dose on the day 28. Osteopontin mRNA expressions were highest in the day 21 groups and lowest in the day 14 groups in the control groups. Interesting results were shown in the day 14 and 21 groups. We found that osteopontin mRNA level was increased in dose dependent manner in the day 14 groups, and decreased dose dependent manner in the day 21 groups. In conclusion, PDGF-BB may have various control effects on type I mRNA expression in the growth and differentiation process of MC3T3-E1 cells and may have contrary regulatory effects on osteopontin mRNA expression. For examples, when the baseline level of osteopontin mRNA was low, as in the day 14, PDGF-BB up-regulated osteopontin mRNA expression in dose dependent manner, and when the baseline level was high as in the day 21, PDGF-BB down-regulated dose dependent manner. Thus, it may be useful for clinical application in periodontal regeneration procedure if further study were performed.

• 대한의용생체공학회:의공학회지
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• 제25권4호
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• pp.309-314
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• 2004

본 연구에서는 세포배양을 위해 개발된 복합생물반응기(Hybrid bioreactor)의 배양조건의 유효성을 평가하고, 3차원 키토산 지지체 (Chitosan-Scaffold Sponge Type)에 배양된 세포에 Hybrid Bioreactor를 이용한 물리적 자극에 대한 반응을 실험하였다. Hybrid bioreactor는 압축변형과 전단변형을 동시에 가할 수 있도록 제작되었다. 본 실험에서는 지지체 크기의 2.5% 변형으로 14일간 150회씩 0.5Hz 로 물리적 자극을 가하였다. 14일간 배양한 세포군은 일정 날짜에 샘플링 (sampling) 하였다. (Day 6, 8, 10, 12, 14). 세포 성장 정도를 알 수 있는 전체 단백질 양을 Lowey의 방범으로 분석하였으며, 분화의 시작을 알리는 표적단백질인 알카라인 포스파타제(Alkaline phosphatase)양을 ELISA로 측정하였다. Hybrid bioreactor를 이용하여 물리적 자극을 가한 군은 자극을 가하지 않은 군에 비하여 표적단백질의 형성을 촉진하였으며, 자극을 가한 관에서 사극을 가하지 않은 군에 비해 빠른 칼슘침착을 나타내었다.

• 대한한의학회지
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• 제24권3호
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• pp.35-44
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• 2003

Objective : This study was performed to evaluate the effects of Palmijihwang-hwan (Baweidehuang-wan) and Obaeja (Galla Rhois) on the regeneration of periodontal tissue. Methods : In this study, we used MC3T3-El cells, such as osteoblast-like cell lines and human periodontal ligament fibroblasts, for experimental material. We separated each type of cells into a control group and an experimental group. In the control group, the cells were cultivated for 48 hours with distilled water and media which contained 10% fetal bovine serum (FBS) and penicillin (l00unit/ml)-streptomycin ($l00{\mu\textrm{g}}/ml$) at $37^{\circ}$ in 5% $CO_2$ gas. In the experimental group, the cells were cultivated for 48 hours with Palmijihwang-hwan extract and Obaeja extract (concentrations $1{\mu\textrm{g}}/ml,{\;}25{\mu\textrm{g}}/ml,{\;}50{\mu\textrm{g}}/ml$) under the same conditions as the control group. Investigating the regeneration of periodontal tissue was performed by evaluating proliferation, the activity of alkaline phosphatase and the synthetic ability of proteins using those cultivated cells by means of microculture tetrazolium (MTT) assay, alkaline phosphatase substrate kit and protein assay kit. Results : 1. In vitro, Palmijihwang-hwan extract increased the proliferation of MC3T3-El cells. 2. In vitro, Obaeja extract increased the activity of alkaline phosphatase and the synthetic ability of protein in MC3T3-El cells and human periodontal ligament fibroblasts depending on Obaeja extract's concentration. Conclusion : Obaeja extract can be developed as a subsidiary medicine for the regeneration of periodontal tissue. Further studies to evaluate the different concentrations the Obaeja extract and clinical trials in vivo are suggested.

• 방사선산업학회지
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• 제6권3호
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• pp.223-229
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• 2012

In this study, we investigated the biological damage and stress responses induced by ion beam (proton beam) irradiation as a basis for the development of protective measures against space radiation. We examined the biological effects of proton beam produced by MC-50 cyclotron at KIRAMS on the cultured cells and mice. The proton beam energy used in this study was 34.9 MeV and the absorption dose rate for cells and mice were $0.509Gy\;sec^{-1}$ and $0.65Gy\;sec^{-1}$, respectively. The cell survival rates measured by plating efficiency showed the different sensitivity and dose-relationship between CHO cells and Balb/3T3 cells. HGPRT gene mutation frequency in Balb/3T3 was $15{\times}10^{-6}Gy^{-1}$, which was similar to the reported value of X-ray. When stress signaling proteins were examined in Balb/3T3 cells, $I{\kappa}B-{\alpha}$ decreased markedly whereas p53, phospho-p53, and Rb increased after proton beam irradiation, which implied that the stress signaling pathways were activated by proton beam irradiation. In addition, cellular senescence was induced in IMR-90 cells. In the experiments with C57BL/6 mouse, the immune cells (white blood cells, lymphocytes) in the peripheral blood were greatly reduced following proton beam irradiation whereas red blood cells and platelets showed relatively little change. These results can be utilized as basic data for studying the biological effects of proton beam using MC-50 cyclotron with respect to proton therapy research as well as space radiation research.