Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Apoptotic Effect of MC Fraction of Trichosanthis Kirilowii Maxim in Human Leukemic U937 Cells

과루인 Methylene Chloride층의 세포고사 유도 효과

  • Lee Ju Ryoung (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Eun Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Cha Yun Yi (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kang In Cheol (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Park Young Doo (School of Biotechnology & Plant Metabolism Research Center, Kyunghee University) ;
  • Ahn Kyoo Seok (Oriental Medical College, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 이주령 (경희대 동서의학대학원 종양학 실험실) ;
  • 이은옥 (경희대 동서의학대학원 종양학 실험실) ;
  • 차윤이 (경희대 동서의학대학원 종양학 실험실) ;
  • 강인철 (경희대 동서의학대학원 종양학 실험실) ;
  • 박영두 (경희대학교 생명공학연구원) ;
  • 안규석 (경희대학교 한의과대학) ;
  • 김성훈 (경희대 동서의학대학원 종양학 실험실)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The Methylene Chloride(MC) fraction of Trichosanthis kirilowii Maxim has been investigated anti-tumor activities in vitro. The MC fraction of Trichosanthis kirilowii Maxim significantly inhibited the proliferation of human leukemic U937 cell with an IC50 of approximately 10μg/ml in a dose-dependent manner. We found that the MC fraction upregulated of caspase9 and caspase-3 activity and cleaved PARP expression but it didn't affect bax and bcl-2. which were demonstrated by western blot analysis. Taken together, these results exerted that the MC fraction suppessed human leukemic U937 cell proliferation by inducing apoptosis, suggesting the MC fraction of Trichosanthis kirilowii Maxim is possible to show anti-cancer activity in vivo.

Keywords

References

  1. Traditional Sin-Japanese Medicine with Color Picture Tsuneo Namba
  2. Phamalogical Action and Application of Anticancer Traditional chinese Medicine
  3. The Oncologist v.4 Apotosis. Lyonel C. Israels, Ester D. Israel.
  4. Immunology(fourth edition) Richard A. Goldsby;Thomas J, Kindt;Barbara A. Osborne
  5. Toxicology Letters v.22 Triptolide induced cytotoxic effect on human promyelocytic leukemia, T cell Iymphoma and human hepatocellular carcinoma cell lines. E. Wai-chins Chan(et al.)
  6. Canc Lett v.121 Apoptosis induced by tea polyphenols in HL-60 cells. Yan Zhao;Jin Cao;Hong Ma;Jianwei Liu.
  7. J. Immunol/ Methods v.65 Rapid colorimetric assat for cellular growth and survival: application to proliferation and cytotoxicity assays. T Mosmann.
  8. J. Immunol. v.184 A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. Vermes I;C. Haanen;H. Steffens-Nakken;C. REutelinfsperger
  9. Tewari(et al.)