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Apoptotic Effect of MC Fraction of Trichosanthis Kirilowii Maxim in Human Leukemic U937 Cells  

Lee Ju Ryoung (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Lee Eun Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Cha Yun Yi (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Kang In Cheol (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Park Young Doo (School of Biotechnology & Plant Metabolism Research Center, Kyunghee University)
Ahn Kyoo Seok (Oriental Medical College, Kyunghee University)
Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.3, 2003 , pp. 643-647 More about this Journal
Abstract
The Methylene Chloride(MC) fraction of Trichosanthis kirilowii Maxim has been investigated anti-tumor activities in vitro. The MC fraction of Trichosanthis kirilowii Maxim significantly inhibited the proliferation of human leukemic U937 cell with an IC50 of approximately 10μg/ml in a dose-dependent manner. We found that the MC fraction upregulated of caspase9 and caspase-3 activity and cleaved PARP expression but it didn't affect bax and bcl-2. which were demonstrated by western blot analysis. Taken together, these results exerted that the MC fraction suppessed human leukemic U937 cell proliferation by inducing apoptosis, suggesting the MC fraction of Trichosanthis kirilowii Maxim is possible to show anti-cancer activity in vivo.
Keywords
MC fraction; Trichosanthis Kirilowii Maxim; apoptosis; U937;
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