• Applied Biological Chemistry
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• 제11권
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• pp.89-93
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• 1969

전보(前報)에서 분리(分離) 선정(選定)한 균주(菌株)로 밀기울 및 밀기울 20% 함유(含有) 왕겨에 배양(培養)하여 만든 발효사료(醱酵飼料)를 산란계(産卵鷄)의 기본사료(基本飼料)에 10% 첨가(添加)하여 사양시험(館養試驗)을 하고 또한 조(粗) cellulase 제(劑) 제조(製造)에 대(對)한 검토(檢討)와 이들균(菌)의 동정(同定)을 한 결과(結果)는 다음과 같다. 1. 선정(選定)한 4균주(菌株)는 MC-9 : Aspergillus niger van Tieghem MC-10 : Aspergillus flavus Link MC-53 : Aspergillus penicilloides Spegazzini MC-61 : Aspergillus niger mut. Schiemanni n. comb. 등(等)과 근연(近緣)의 균주(菌株)이었다. 2. 선정(選定) 균주(菌株)의 밀기울 배양(培養)에서 얻은 조효소제(粗酵素劑) 의 수량(收量)은 배양풍건물(培養風乾物)에 대(對)하여 MC-9에서는 2.9 %, MC-10 : 3.9%, MC-53 : 6.4%, MC-61 : 2.6 % 등(等)이었으며 이들 조효소제(粗酵素劑)의 역가(力價)도 밀기울 배양물(培養物) 추출(抽出) 효소액(酵素液)에서와 같이 MC-9의 것이 가장 강(强) 하였다. 3. 사양시험(飼養試驗) 결과(結果), 체중변화(體重變化)에 있어서는 대조군(對照群) 에 비(比)하여 유의차(有意差)가 없었으나 산란율(産卵率)은 MC-9 및 MC-61의 발효사료(醱酵飼料) 급이군(給餌群)에서 P<0.05 수준(水準)의 증가(增加) 유의차(有意差)를 나타내었다.

• Archives of Pharmacal Research
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• 제16권4호
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• pp.276-282
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• 1993

This study has been undertaken to examine the effect of 3-methylcholanthrene (3MC) on rat uterine growth and to understand the mechanism of action of 3MC in rat uterus. After diethylstilbesterol(DES) or tamoxifen(TAM) or 3MC or DES plus TAM or DES plus 3MC was administered into immature female rats, uterine weight over corn oil-treated uteri. 3MC treatment had no effect on uterine weight but, DES stimulated uterine weight was inhibited by 3MC concomitant tratment. While TAM alone treatment showed slight increase in uterine wieght, inhibited uterine growth simulated by DES when it was adiministrated with DES condirect binding assay with $[^3H]$ estradiol and the relative binding affinities of 3MC and TAM were estimated by competetion assy. Estradiol tumed out to have high affinity for rat uterine estrogen receptor (kd = 0.4 nM). The relative binding affinities of TAM and 3MC were 1% and 4.7% that of DES for rat uterine estrogen receptor, respectively. 3MC was shown to have similar affinity for eat uterine estrogen receptor to that of TAM. Effects of DES 3MC and TAM administration in vivo on rat uterine estrogen recptor level were examined. It was confirmed that the estrogen, DES and antiestrogen, TAM decreased estrogen receptor levels from rat ulterus and also 3MC decreased rat uterine estrogen receptor level when rats were treated with DES, TAM and 3MC in vivo. Data indicates that 3MC acts as an antiestrogen mediated through estrogen receptor system.

• 농업과학연구
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• 제51권1호
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• pp.87-95
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• 2024

The objective of this study was to investigate the anti-obesity effects of adding Momordica charantia (MC) and Chrysanthemum zawadskii var. latilobum (CZ) extracts to drinking water on obesity-induced mice. A total of 84 eight-week-old C57BL/6N male mice with an initial body weight (BW) of 28.11 ± 1.39 g were used in this study. All treatments were fed a high-fat diet for d 28. Mice were randomly divided into seven drinking treatments (six replicate each treatment) based on the initial BW. Treatments are as follows: control (CON), normal tap water, MC 1, CON with 1% MC aqueous extract, MC 2, CON with 2% MC aqueous extract, CZ 1, CON with 1% CZ aqueous extract, CZ 2, CON with CZ aqueous extract (2%), MCZ 1, CON with 1% MC aqueous extract and 1% CZ aqueous extract, MCZ 2, CON with 2% MC aqueous extract and 2% CZ aqueous extract. During the entire period, the CZ 1, MCZ 1, and MCZ 2 significantly decreased (p < 0.05) gain to feed than CON. The CON significantly higher (p < 0.05) water intake than other treatments on d 0 to 14. The MCZ 1 significantly decreased (p < 0.05) relative (ratio of absolute organ weight to BW) organ weights, including retroperitoneal white adipose tissue (RWAT) weight and inguinal white adipose tissue (IWAT) weight, compared to CON. In conclusion, our study suggests that there was no significant difference in the anti-obesity effects between MC and CZ, and MCZ 1 has synergistic effects by regulating adipose tissue.

• 인터넷정보학회논문지
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• 제21권5호
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• pp.1-7
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• 2020

강화학습에서 근사함수로써 사용되는 딥 인공 신경망은 이론적으로도 실제와 같은 근접한 결과를 나타낸다. 다양한 실질적인 성공 사례에서 시간차 학습(TD) 은 몬테-칼로 학습(MC) 보다 더 나은 결과를 보여주고 있다. 하지만, 일부 선행 연구 중에서 리워드가 매우 드문드문 발생하는 환경이거나, 딜레이가 생기는 경우, MC 가 TD 보다 더 나음을 보여주고 있다. 또한, 에이전트가 환경으로부터 받는 정보가 부분적일 때에, MC가 TD보다 우수함을 나타낸다. 이러한 환경들은 대부분 5-스텝 큐-러닝이나 20-스텝 큐-러닝으로 볼 수 있는데, 이러한 환경들은 성능-퇴보를 낮추는데 도움 되는 긴 롤-아웃 없이도 실험이 계속 진행될 수 있는 환경들이다. 즉, 긴롤-아웃에 상관없는 노이지가 있는 네트웍이 대표적인데, 이때에는 TD 보다는 시간적 에러에 견고한 MC 이거나 MC와 거의 동일한 학습이 더 나은 결과를 보여주고 있다. 이러한 해당 선행 연구들은 TD가 MC보다 낫다고 하는 기존의 통념에 위배되는 것이다. 다시 말하면, 해당 연구들은 TD만의 사용이 아니라, MC와 TD의 병합된 사용이 더 나음을 이론적이기 보다 경험적 예시로써 보여주고 있다. 따라서, 본 연구에서는 선행 연구들에서 보여준 결과를 바탕으로 하고, 해당 연구들에서 사용했던 특별한 리워드에 의한 복잡한 함수 없이, MC와 TD의 밸런스를 랜덤하게 맞추는 좀 더 간단한 방법으로 MC와 TD를 병합하고자 한다. 본 연구의 MC와 TD의 랜덤 병합에 의한 DQN과 TD-학습만을 사용한 이미 잘 알려진 DQN과 비교하여, 본 연구에서 제안한 MC와 TD의 랜덤 병합이 우수한 학습 방법임을 OpenAI Gym의 시뮬레이션을 통하여 증명하였다.

• 한국동물위생학회지
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• 제31권3호
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• pp.369-374
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• 2008

The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

• 농업과학연구
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• 제51권1호
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• pp.53-61
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• 2024

A total of 180 21-day-old weaning pigs ([Yorkshire × Landrace] × Duroc) with an initial body weight of 6.44 ± 0.01 kg were randomly assigned to 9 treatments for evaluating the effects of replacing dietary inorganic copper (Cu) and zinc (Zn) with glycine (Gly) or methionine (Met)-chelated Cu and Zn on growth performance and nutrient digestibility. The experimental period was 35 days. There were four replicated pens per treatment, with five pigs (three males and two females) per pen. Dietary treatments consisted of a basal diet (CON), in which the sources of Cu and Zn were in inorganic form. The inorganic Cu and Zn in the basal diet were replaced by glycine-chelated (GC) and methionine-chelated (MC) Cu and Zn by 30, 50, 70, or 100% to form the GC1, GC2, GC3, GC4, or MC1, MC2, MC3, MC4 groups. The 100% replacement of dietary inorganic Cu and Zn with GC or MC increased (p < 0.05) average daily gain, average daily feed intake, and gain-to-feed ratio. The complete replacement of dietary inorganic Cu and Zn with GC or MC led to enhanced (p < 0.05) digestibility of dry matter, nitrogen, Cu and Zn. Thus, the replacement of inorganic Cu and Zn with GC or MC can improve the growth efficiency and nutrient utilization of weaning pigs.

• 한국자기공명학회논문지
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• 제16권1호
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• pp.34-45
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• 2012

Melanocortin-4 receptor (MC4R) subtype is associated with obese humans. Especially, in a patient with severe early-onset obesity, novel heterozygous mutation in the MC4R gene was detected, resulting in an exchange of aspartic acid to asparagine in $90^{th}$ amino acid residue located in the predicted second trans-membrane domain (TM2). Mutations in the melanocortin-4 receptor (MC4R) gene are the most frequent monogenic causes of severe obesity which have been described as heterozygous with loss of function. In order to compare structure difference between MC4R wild type (MC4R-TM2-wt) and mutant (MC4R-TM2-D90N), we designed both MC4R-TM2-wt and MC4R-TM2-D90N construct in pET 21b vector. In this study, we optimized high-yield purification procedure for recombinant TM2-D90N. Eluted recombinant protein was resolubilized under urea condition for thrombin cleavage reaction and we conducted the high-performance liquid chromatography (HPLC) with reverse phase column under 1% acetonitrile, 0.01% TFA buffer solution. The molecular size of purified target peptide was confirmed by Tricine-SDS page analysis. To characterize MC4R-TM2-D90N, we have performed $^{15}N$-isotope labeling of peptide using M9 media and purified labeled target peptide for hetero-nuclear NMR spectroscopy.

• 생명과학회지
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• 제32권2호
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• pp.125-134
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• 2022

본 연구는 노니(Morinda citrifolia)에 함유된 주요 생리활성물질이 RAW 264.7 세포에서 JAK/STAT 신호전달 경로를 통하여 항염증 작용에 관여하는 것을 조사하였다. 건조된 M. citrifolia 열매의 MeOH 추출에 의한 유기용매의 순차 분획에서 얻은 EtOAc 분획물(Mc-EtOAc)에서 가장 높은 항산화 활성을 확인하였고, H₂O₂로 유도된 RAW 264.7 세포의 산화적 스트레스에 대한 세포보호 효과는 처리 농도의존적으로 세포독성을 차단하였다. LPS 처리로 71.6%의 RAW 264.7 세포 생존율에서 240 ㎍/ml의 Mc-EtOAc를 전처리한 군에서 84.5%의 세포 생존율 증가를 보였다. LPS로 유도된 RAW 264.7 세포의 NO 생성 저해활성은 240 ㎍/ml의 Mc-EtOAc에서 양성 대조군의 절반 수준으로 NO의 생성양을 감소시켰다. Mc-EtOAc 처리로 iNOS의 발현량은 농도의존적으로 유의하게 감소하였고, COX-2의 발현은 LPS 유도로 3배 정도로 증가되었으나, 120, 240 ㎍/ml의 Mc-EtOAc를 전처리시 농도의존적으로 유의하게 감소시켰다. 또한 pro-inflammatory cytokine IL-1β와 TNF-α의 mRNA 발현도 억제하였다. 이러한 Mc-EtOAc의 항염증 작용이 상위 신호전달 경로인 JAK/STAT 신호전달 경로 통해 일어나지를 조사한 결과, Mc-EtOAc의 전처리로 LPS로 유도된 RAW 264.7 세포에서 pJAK1과 pSTAT3의 인산화 정도가 유의성 있게 감소하였다. 따라서 RAW 264.7 세포에서 Mc-EtOAc의 항염증 효과는 JAK1/STAT3 신호전달 경로를 통해 염증반응을 억제하는 것으로 사료된다.

• 대한본초학회지
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• 제30권1호
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• pp.95-101
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• 2015

Objectives : The aim of this study was to confirm whether or not coral has a preventive effect on development of atopic dermatitis induced by house mite(dermatophagoides pteronyssinus) in NC/Nga mice. Methods : This study was undertaken by using a reliable Atopic dermatitis mouse model demonstrating similar immune response. Lobophytum crassum was administered orally to NC/Nga mouse for 3 weeks. In order to verify the effectiveness of Lobophytum crassum in atopic dermatitis treatment, its role in immune factors were observed in NC/Nga mice. Results : ALT, AST, BUN and creatine levels were all within in the normal ranges in MC-1 200 and 400 (mg/kg) treated groups, indicating no induced toxicity. MC-1 200 and 400 (mg/kg) groups decreased of atopic dermatitis skin manifestation in NC/Nga mouse of MC-1 200 and 400 (mg/kg) groups compared to that of the control group and decreased the ratio of WBC and lymphocyte in blood. Also, MC-1 200 and 400 (mg/kg) groups significant decreased the ratio of CD4+, CD8+, CD11b+/Gr1+, B220/CD23 and CD4/CD25 immune cell ratio in ALN. Finally MC-1 200 and 400 (mg/kg) groups significantly increased the ratio of CD4+, CD8+, B220/CD23 and CD4/CD25 immune cells in DLN. Conclusions : Theses results suggested that Lobophytum crassum has suppressive effects on aberrant and overactive immunological activities in dermatophagoides pteronyssinus-induced dermatitis mice of NC/Nga.

• 미생물학회지
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• 제29권1호
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• pp.16-24
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• 1991

The yeast gene THR1 encodes the homoserine kinase (EC 2.7.1.39: HKase) which catalyses the first step of the threonine specific arm at the end of the common pathway for methionine and threonine biosynthesis. A recombinant plasmid pMC3 (12.6 kilobase pairs, vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of a thr1 mutation in a yeast recipient strain M39-1D. When subcloned into pMC32 (8.6kbp, vector YRp7) and pMC35 (8.3 kbp, vector YIp5), the HindIII fragment (2.7 kbp) of pMC3 insery was positive in the thrI complementing activity in both yeast and E. coli auxotrophic strains. The linearized pMC35 was introduced into the original recipient yeast strain and the mitotically stable chromosomal integrant was identified among the transformants. Through the tetrad analysis, the integration site of the pMC35 was localized to the region of THR1 structural gene at an expected genetic distance of approximately 11.1 cM from the ARG4 locus on the right arm of the yeast chromosome VIII. When episomically introduced into the auxotrophic cells and cultured in Thr omission liquid medium, the cloned gene overexpressed the HKase in the order of thirteen to fifteenfold, as compared with a wildtype. HKase levels are repressed by addition of threonine at the amount of 300 mg/l and 1, 190 mg/l for pMC32 and pMC3, respectively. Data from genetic analysis and HKase response thus support that the cloned HindIII yeast DNA fragment contains the yeast thr1 structural gene, along with necessary regulatory components for control of its proper expression.