• Journal of Microbiology and Biotechnology
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• v.26 no.3
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• pp.603-609
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• 2016

Brucellosis affects a wide range of host species, including humans and many livestock animals. Chronic infections of the disease make antibiotic treatment costly, and the current vaccine used in livestock has not been approved for human use. This study investigated the possible use of the Brucella abortus outer membrane protein A (OmpA) as a candidate subunit vaccine in an infected mouse model. The ompA gene was cloned and overexpressed, and the recombinant OmpA (rOmpA) protein fused to maltose binding protein (MBP) was purified in Escherichia coli. Immunogenicity was verified through western blotting, and mice were immunized and challenged to evaluate its protective effect. Mice treated with rOmpA exhibited induced humoral and host cell-mediated responses, with a significant increase in immunoglobulin G (IgG1 and IgG2a) and cytokine levels, especially TNF-α and IL-12, compared with the control groups treated with either MBP or PBS. In conclusion, rOmpA should be highly considered as a future subunit vaccine for brucellosis, and further studies regarding rOmpA and its protective ability are suggested.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.46 no.4
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• pp.17-22
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• 2009

FlexRay is a new standard of network communication system which provides solutions to the degradation problems generated by many ECU (Electronic Control Unit) connections in automobiles and automation systems. The upper bound of the data rate is 10Mbps and it provides two channels for redundancy In this paper, FlexRay system is first designed using SDL. For hardware implementation, FlexRay system is designed using Verilog HDL based on the SDL design result. The designed system is synthesized using Synopsys Design Compiler with the Magna/Hynix 0.18 um cell library. In this paper, to construct a FlexRay network, active star is used since active star systems can provide high speed data transmission up to 10Mbps. The performance of the star network is tested using one transmitter node and two receiver nodes.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.1C
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• pp.112-121
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• 2002

In this paper, we present a design of a hash processor for data security systems. Two standard hash algorithms, Sha-1(American) and HAS-1600(Korean), are implemented on a single hash engine to support real time processing of the algorithms. The hash processor can also be used as a PRNG(Pseudo-random number generator) by utilizing SHA-1 hash iterations, which is being used in the Intel software library. Because both SHA-1 and HAS-160 have the same step operation, we could reduce hardware complexity by sharing the computation unit. Due to precomputation of message variables and two-stage pipelined structure, the critical path of the processor was shortened and overall performance was increased. We estimate performance of the hash processor about 624 Mbps for SHA-1 and HAS-160, and 195 Mbps for pseudo-random number generation, both at 100 MHz clock, based on Samsung 0.5um CMOS standard cell library. To our knowledge, this gives the best performance for processing the hash algorithms.

• Journal of Internet Computing and Services
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• v.18 no.5
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• pp.1-8
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• 2017

Lightweight Encryption Algorithm(LEA) was developed by National Security Research Institute(NSRI) in 2013 and targeted to be suitable for environments for big data processing, cloud service, and mobile. LEA specifies the 128-bit message block size and 128-, 192-, and 256-bit key sizes. In this paper, block cipher LEA algorithm which can encrypt and decrypt 128-bit messages is designed using Verilog-HDL. The designed IP for encryption and decryption has a maximum throughput of 874Mbps in 128-bit key mode and that of 749Mbps in 192 and 656Mbps in 256-bit key modes on Xilinx Vertex5. The cryptographic IP of this paper is applicable as security module of the mobile areas such as smart card, internet banking, e-commerce and IoT.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.6A
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• pp.640-647
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• 2006

This paper describes a design of AES-based CCMP(Counter mode with CBC-MAC Protocol) core for IEEE 802.11i wireless LAN security. To maximize the performance of CCMP core, two AES cores are used, one is the counter mode for data confidentiality and the other is the CBC node for authentication and data integrity. The S-box that requires the largest hardware in ARS core is implemented using composite field arithmetic, and the gate count is reduced by about 27% compared with conventional LUT(Lookup Table)-based design. The CCMP core was verified using Excalibur SoC kit, and a MPW chip is fabricated using a 0.35-um CMOS standard cell technology. The test results show that all the function of the fabricated chip works correctly. The CCMP processor has 17,000 gates, and the estimated throughput is about 353-Mbps at 116-MHz@3.3V, satisfying 54-Mbps data rate of the IEEE 802.11a and 802.11g specifications.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.2
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• pp.144-149
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• 2001

For the production and purification of a single chain human insulin precursor, four types of fusion peptides $\beta$-galactosidase (LacZ), maltose binding protein (MBP), glutathione-S-transferase (GST), and (His)(sub)6-tagged sequence (HTS) were investigated. Recombinant E. coli harboring hybrid genes was cultivated at 37$\^{C}$ for 1h, and gene induction occurred when 0.2mM of isopropyl-D-thiogalactoside (IPTG) was added to the culture broth, except for E. coli BL21 (DE3) pLysS harboring a pET-BA cultivation with 1.0mM IPTG, followed by a longer than 4h batch fermentation respectively. DEAE-Sphacel and Sephadex G-200 gel filtration chromatography, amylose affinity chromatography, glutathione-sepharose 4B affinity chromatography, and a nickel chelating affinity chromatography system as a kind of immobilized metal ion affinity chromatography (IMAC) were all employed for the purification of a single chain human insulin precursor. The recovery yields of the HTS-fused, GST-fused, MBP-fused, and LacZ-fused single chain human insulin precursors resulted in 47%, 20%, 20%, and 18% as the total protein amounts respectively. These results show that a higher recovery yield of the finally purified recombinant peptides was achieved when affinity column chromatography was employed and when the fused peptide had a smaller molecular weight. In addition the pET expression system gave the highest productivity of a fused insulin precursor due to a two-step regulation of the gene expression, and the HTS-fused system provided the highest recovery of a fused insulin precursor based on a simple and specific separation using the IMAC technique.

• Journal of Acupuncture Research
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• v.19 no.1
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• pp.147-158
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• 2002

Objective : The aim of the present study is to investigate the effect of stimulation on different sites of the body on MBP(mean blood pressure) and HR(heart rate). Methods : Six healthy men have participated in this study. Before and after 10 min, exercise on a running machine of l0km/hr, acupressure, plain acupuncture and electroacupuncture(50Hz) stimulation was practiced on GV20, LI4, ST36, BL40 and non-acupoints on T4 and T10 respectively for 20 min. and in a control group without any treatment. The changes of MBP and HR after exercise have been observed for 20 min. at 5 minute intervals. Results : Compared with control, no significant difference was observed in research of the blood pressure measurement regardless of methods nor sites of stimulation. But there were trends of reduction in the heart rates in all experimental groups. Especially, in the group of acupressure on T10 before exercise, GV20, T10 after exercise and electroacupuncture on GV20, LI4, BL40 after exercise there was statistically significant decreases in heart rates. Conclusion : From the present experiment, it is concluded that somatic stimulation has effect on the heart rates but not on the blood pressures, and the presence of effective sites on the decreases of heart rates suggest that this effect may depend on sites of stimulation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.4B
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• pp.391-398
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• 2011

In this paper, we propose a MAC protocol for visible light wireless LAN using LED light. The proposed MAC protocol has a function of automatic direction optimization for LED transceiver. There is periodical time slot for broadcasting and registration in the proposed MAC protocol. We used polling method for data transfer in the protocol since visible light is hard to detect carrier and collision by other mobile stations; thus we cannot use CSMA/CD method in visible light LAN. We also amended the MAC frame of IEEE 802.11 to propose a revised version for visible light wireless LAN using LED light. We investigate the performance of the proposed MAC protocol. When the bit rate of physical layer is 100 Mb/s, the net data throughput using the proposed MAC protocol is estimated to 94.386 Mb/s.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.1
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• pp.1113-1116
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• 2005

Using COMSAT which provides multi-beam switching function, the optimal transmission channel environment which provides the high data rate communication are proposed in this paper. Also the link budget for communication transponder of COMSAT is designed. Based on the channel modeling for group delay, non-linear and gain flatness characteristics, the system performances which provide various data rate services were analyzed in Ka-band satellite channel. As the transmission data rate is increased, the degradation due to these channel characteristics is severely increased. The linear component of group delay and the AM-AM component of non-linear characteristics severely affect the system performance. To efficiently provide the various service via the same transmission system it is necessary to equalize the primary impairment factors. The optimum operating points of HDR satellite transmission system are implemented and operated by considering the analyzed results on channel characteristics.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.5
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• pp.1015-1022
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• 2012

This paper deals with hardware design which unifies MD5 and HAS-160 hash algorithms. Two algorithms get a message with arbitrary length and process message blocks divided into 512 bits each time and output a hash code with a fixed length. MD5 ouputs a hash code of 128 bits and HAS-160 a hash code of 160 bits. The unified hash core designed has 32% of slices overhead compared to HAS-160 core. However, there is only a fixed message buffer space used. The unified hash core which run a step in one clock cycle operates at 92MHz and has performance which digests a message in the speed of 724Mbps at MD5 and 581Mbps at HAS-160 hash mode. The unified hash core which is designed can be applicable to the areas such as E-commerce, data integrity and digital signature.