• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1383-1388
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• 2008

Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.337-343
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• 2004

A bacterial strain concurrently producing extracellular chitosanase and chitinase was isolated from soil and identified as a member of the $\beta$-subgroup of Proteobacteria through its 16S rRNA analysis and some biochemical analyses. The newly discovered strain, named as KNU3, had 99% homology of its 16S rRNA sequence with chitinolytic $\beta$-Proteobacterium CTE108. Strain KNU3 produced 34 kDa of chitosanase in addition to two chitinases of 68 kDa and 30 kDa, respectively. The purified chitosanase protein (ChoK) showed activity toward soluble, colloidal, and glycol chitosan, but did not exhibit any activity toward colloidal chitin. The optimum pH and temperature of ChoK were 6.0 and $70^{\circ}C$, respectively. The chitosanase was stable in the pH 4.0 to 8.0 range at $70^{\circ}C$, while enzyme activity was relatively stable at below $45^{\circ}C$. MALDI-TOF MS and N-terminal amino acid sequence analyses indicated that ChoK protein is related to chitosanases from Matsuebacter sp. and Sphingobacterium multivorum. HPLC analysis of chitosan lysates revealed that glucosamine tetramers and hexamers were the major products of hydrolysis.

• Journal of Microbiology
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• 제45권3호
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• pp.268-271
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• 2007

In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDI-TOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

• Elastomers and Composites
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• 제43권1호
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• pp.31-38
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• 2008

[ $FeCl_3$ ]를 첨가한 초음파 조건에서 4-nitroaniline, 3-nitroaniline, and 4-isopropylaniline등의 방향족 아민 화합물과 풀러렌 산화물$[C_{70}(O)_n](n\geq1)$을 반응시켰다. 이 반응은 방향족 아민 화합물을 사용한 풀러렌 산화물 쪼개짐 반응이다. MALDI-TOF-MS and UV-vis spectra를 사용하여 생성된 화합물이 아민화 풀러렌 유도체임을 확인하였다.

• 대한의생명과학회지
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• 제29권4호
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• pp.256-262
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• 2023

The purpose of this study is to investigate microbial contamination in water purifiers from two universities (A and B) in Chungcheong region and to evaluate about the harmfulness of the isolated bacteria to the human. The degree of microbiological contamination of six water purifiers at university A was investigated three times from July 2018 to September 2019, and nine water purifiers at university B were investigated in 2023. The isolated bacteria were biochemically identified using an API kit and Vitek-2 system, and then the bacteria were identified to the species level using MALDI-TOF MS. In addition, the possibility of human infection of the isolated bacteria was evaluated through a literature search. In July 2018 and September 2019, the number of bacteria isolated inside the faucet was below the acceptable standard for hot water, but exceed for cold water in all water purifiers. In January and September 2019, bacteria exceeding the acceptable standards were isolated nine times from the cold water of six water purifies (a total of 12 water purifiers). Bacteria identified by MALDI-TOF MS included anaerobic bacteria (Clostridium novyi, Clostridium themopalmarium etc.), Gram-positive bacilli (Microbacterium testaceum, Arthrobacter woluwensis etc.), and Gramnegative bacilli (Acinetobacter nosocomialis, Comamonas kerstersii etc.), which are difficult identify by biochemical methods. In conclusion, bacteria exceeding the acceptable standard were isolated from the cold water of most of the water purifiers. Most of the isolated bacteria were low-pathogenic bacteria from natural environment, but opportunistic bacteria that can cause infection in humans were also isolated from some water purifiers.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6435-6439
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• 2012

Chemoresistance to cancer therapy is a major obstacle to the effective treatment of human cancers with cisplatin (DDP), but the mechanisms of cisplatin-resistance are not clear. In this study, we established a cisplatin-resistant human ovarian cancer cell line (COC1/DDP) and identified differentially expressed proteins related to cisplatin resistance. The proteomic expression profiles in COC1 before and after DDP treatment were examined using 2-dimensional electrophoresis technology. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and high performance liquid chromatography-electrospray tandem MS (NanoUPLC-ESI-MS/MS). 5 protein spots, for cytokeratin 9, keratin 1, deoxyuridine triphosphatase (dUTPase), aarF domain containing kinase 4 (ADCK 4) and cofilin1, were identified to be significantly changed in COC1/DDP compared with its parental cells. The expression of these five proteins was further validated by quantitative PCR and Western blotting, confirming the results of proteomic analysis. Further research on these proteins may help to identify novel resistant biomarkers or reveal the mechanism of cisplatin-resistance in human ovarian cancers.

• Journal of Forest and Environmental Science
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• 제26권2호
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• pp.83-94
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• 2010

Chinese fir (Latin name: Cunninghaimia lanceolata) is one of the major commercial coniferous trees. Most of Chinese fir forests are managed in successive rotation sites, which lead productivity to decline. Autotoxicity is the important reason for soil degradation of Chinese fir plantation, especially, phenolic acids are considered as the major allelopathic toxins which induce autotoxicity in Chinese fir rotation stands. We performed here proteomic approach to investigate the response of proteins in Chinese fir leaves to salicylic acid. The tube plantlets of Chinese fir clone were treated with 120 mg/L salicylic acid for 1, 3 and 5th day. 2-DE, coupled with MALDI-TOF-TOF/MS, was used to separate and identify the responsive proteins. We found 12, 7, and 12 candidate protein spots that were up- or down-regulated by at least 2.5 fold after 1, 3, and 5th day of the stress, respectively. Of these protein spots, 16 spots were identified successfully. According to the putative physiological functions, these proteins were categorized into five classes (1) the proteins involved in protein stability and folding, including 26S proteome, Grp78, Hsp70, Hsp90 and PPIase; (2) the protein involved in photosynthesis and respiration, including OEC 33 kDa subunit, GAPDH; (3) the protein related to cell endurance to acid, F-ATPase; (4) the protein related to cytoskeleton, tubulin; (5) the protein related to protein translation: prolyl-tRNA synthetase. These results give new insights into autotoxic substance stress response in Chinese fir leaves and provide preliminary footprints for further studies on the molecular signal mechanisms induced by the stress.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.724-724
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• 2003

• 미생물학회지
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• 제53권3호
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• pp.163-169
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• 2017

복숭아 수확시기에 낙과한 토양에서 Lactobacillus sp. D4와 D5 균주를 분리하였다. 분리한 Lactobacillus sp. D4와 D5 균주를 동정하기 위하여 형태학적 동정, 생화학적 동정 및 16S rRNA 유전자서열 분석을 수행하였다. 16S rRNA 유전자서열 분석 결과 Lactobacillus sp. D4는 Lactobacillus plantarum subsp. plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $40997^T$에 각각 99.05%, 98.98% 일치하였으며, Lactobacillus sp. D5는 Lactobacillus pentosus ATCC $40997^T$, Lactobacillus plantarum subsp. plantarum ATCC $14917^T$에 각각 98.71%, 98.64% 일치하였다. Lactobacillus sp. D4와 D5 균주는 당 이용성 비교에서 Lactobacillus plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $8041^T$에 비교하여 다른 결과를 나타내었다. 정확한 동정을 위하여 VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) 분석, multiplex PCR, random amplified polymorphic DNA (RAPD)-PCR을 수행하였다. 이러한 결과에 근거하여 Lactobacillus sp. D4와 D5 균주는 Lactobacillus plantarum으로 동정되었다.

• 한국식품과학회지
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• 제40권6호
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• pp.712-716
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• 2008

Salmonella로부터 식품 안전성을 높이기 위한 보존법의 병용처리에 의한 효과를 평가하고자 S. Typhimurium을 열과 산, 산화제 등으로 연속 처리한 후 생균수를 측정하여 효과를 분석하였다. 그리고 열충격에 의하여 S. Typhimurium 내에 발현되거나 억제되는 단백질을 이차원 전기영동과 MALDI-TOF 질량분석기로 분석하였다. 열처리된 S. Typhimurium은 초산과 염산의 pH 4에서의 생균수가 1.3-1.8 log CFU/mL가 줄었고 비열처리 S. Typhimurium은 생균수가 약 5 log CFU/mL가 감소하였다. 열처리 S. Typhimurium은 butyl hydrogen peroxide와 과산화수소에서 생균수가 1.1-1.7 log CFU/mL가 줄었으나 비열처리 S. Typhimurium은 5.4-5.6 log CFU/mL 감소하였다. 충분하지 않은 사멸 열처리는 S. Typhimurium의 생존력을 증가시키고 산과 산화제 등의 보존제에서 저항성이 커지는 것을 알 수가 있었다. 이차원 전기영동과 MALDI-TOF 질량분석에 의한 발현 단백질 분석 결과 비열처리 S. Typhimurium은 17개의 단백질이 검출되었고 열처리 S. Typhimurium에는 13개의 단백질만 검출되었다. 이들 중에 열충격 단백질로 알려진 DnaK, small heat shock protein 등이 검출되었고 이들이 산과 산화제에서의 생존 저항성 증가와 관련이 있을 것으로 보인다. 그러므로 열처리를 포함하는 hurdle technology를 적용하여 식품을 보존처리할 때 다른 보존제에 대한 교차보호성이 증가되는 사실을 고려하여 적절한 열처리가 고려되어야 된다는 것을 알 수 있었다.