Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage

  • Ryoo, Sung-Weon (Department of Microbiology, Korean Institute of Tuberculosis) ;
  • Park, Young-Kil (Department of Microbiology, Korean Institute of Tuberculosis) ;
  • Park, Sue-Nie (National Center for Toxicological Research, Korea Food and Drug Administration) ;
  • Shim, Young-Soo (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Liew, Hyun-Jeong (Department of Pharmacology, College of Medicine, Seoul National University) ;
  • Kang, Seong-Man (Graduate School of Biotechnology, Korea University) ;
  • Bai, Gill-Han (Department of Microbiology, Korean Institute of Tuberculosis)
  • Published : 2007.06.30
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Abstract

In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDI-TOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

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References

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