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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage  

Ryoo, Sung-Weon (Department of Microbiology, Korean Institute of Tuberculosis)
Park, Young-Kil (Department of Microbiology, Korean Institute of Tuberculosis)
Park, Sue-Nie (National Center for Toxicological Research, Korea Food and Drug Administration)
Shim, Young-Soo (Department of Internal Medicine, College of Medicine, Seoul National University)
Liew, Hyun-Jeong (Department of Pharmacology, College of Medicine, Seoul National University)
Kang, Seong-Man (Graduate School of Biotechnology, Korea University)
Bai, Gill-Han (Department of Microbiology, Korean Institute of Tuberculosis)
Publication Information
Journal of Microbiology / v.45, no.3, 2007 , pp. 268-271 More about this Journal
Abstract
In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDI-TOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
Keywords
proteomic analysis; Mycobacterium tuberculosis K-strain; human monocytic cell line U-937; 2-D PAGE; MALDI-TOF-MS; Mb1363; MT2656;
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