• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.668-673
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• 2013

A recombinant E. coli co-expressing ALA synthase (hemA), NADP-dependent malic enzyme (maeB), and dicarboxylic acid transporter (dctA) was reported to synthesize porphyrin derivatives including iron-containing heme. To enhance the synthesis of bacterial heme, five genes of the porphyrin biosynthetic pathway [pantothenate kinase (coaA), ALA dehydratase (hemB), 1-hydroxymethylbilane synthase (hemC), uroporphyrinogen III synthase (hemD), and uroporphyrinogen III decarboxylase (hemE)] were amplified in the recombinant E. coli co-expressing hemA-maeB-dctA. Pantothenate kinase expression enabled the recombinant E. coli to accumulate intracellular CoA. Intracellular ALA was the most enhanced by uroporphyrinogen III synthase expression, porphobilinogen was the most enhanced by ALA dehydratase expression, uroporphyrin and coproporphyrin were the most enhanced by 1-hydroxymethylbilane synthase expression. The strain co-expressing coaA, hemA, maeB, and dctA produced heme of $0.49{\mu}mol/g$-DCW, which was twice as much from the strain without coaA expression. Further pathway gene amplifications for the porphyrin derivatives are discussed based on the results.

• Environmental Engineering Research
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• 제21권2호
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• pp.171-179
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• 2016

This study analyzes guidelines to select optimum number of grids to represent behavior of a given water system appropriately. The EFDC model was chosen as a 3-D hydrodynamic and water quality model and salt was chosen as a surrogate variable of pollutant. The model is applied to an artificial canal that receives salt water from coastal area and fresh water from a river from respective gate according to previously developed gate operation rule. Grids are subdivided in vertical and horizontal (longitudinal) directions, respectively until no significant changes are found in salinity concentrations. The optimum grid size was determined by comparing errors in average salt concentrations between a test grid systems against the most complicated grid system. MSE (mean squared error) and MAE (mean absolute error) are used to compare errors. The CFL (Courant-Friedrichs-Lewy) number was used to determine the optimum number of grid systems for the study site though it can be used when explicit numerical method is applied only. This study suggests errors seem acceptable when both MSE and MAE are less than unity approximately.

• 대한가정학회지
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• 제44권10호
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• pp.91-100
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• 2006

This study analyzed the formal characteristics of traditional Jokakbo and modern works applying Jokakbo, and developed aroma chumoni representing the symbolic image and cultural identity of Bamboo at Dam-Yang, Mae-Wha at Kwang-Wang and San-Soo-You at Ku-Rae, all of which are in Chonnam. Initially, inform a theoretical point of view, the characteristics of modern works applying Jokakbo were investigated with regard to the pattern, color, fabric material and technique. Secondly, for the development of aroma chumoni, square and round patterns, representing the images of Bamboo, Mae-Wha and San-Soo-You, were applied. Relating to colors, green, pink, red purple, yellow, light yellow red and pale yellow were applied. With respect to the fabric materials, No-Bang and A-Sa, representing the lightness and coolness of the image, were used. When considering the technique, the traditional needle, rather than any other modern technique, was used.

• Journal of Microbiology and Biotechnology
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• 제17권9호
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• pp.1579-1584
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• 2007

5-Aminolevulinate (ALA) synthase (E.C. 2.3.1.37), which mediates the pyridoxal phosphate-dependent condensation of glycine and succinyl-CoA, encoded by the Rhodobacter sphaeroides hemA gene, enables Escherichia coli strains to produce ALA at a low level. To study the effect of the enhanced C4 metabolism of E. coli on ALA biosynthesis, NADP-dependent malic enzyme (maeB, E.C. 1.1.1.40) was coexpressed with ALA synthase in E. coli. The concentration of ALA was two times greater in cells coexpressing maeB and hemA than in cells expressing hemA alone under anaerobic conditions with medium containing glucose and glycine. Enhanced ALA synthase activity via coupled expression of hemA and maeB may lead to metabolic engineering of E. coli capable of large-scale ALA production.

• 인터넷정보학회논문지
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• 제8권6호
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• pp.95-102
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• 2007

협력적 필터링을 이용한 추천시스템은 희소성의 문제로 인해 예측의 정확도에 대한 신뢰성에 문제가 있다. 이는 선호도 평가치의 희소성이 크면 이웃선정과정에 문제가 있을 뿐만 아니라 예측의 정확도를 떨어뜨린다. 본 논문에서는 사용자의 응답 희소성에 따른 MAE의 변화를 조사하였으며 희소성에 따라 집단을 분류하고 분류된 집단에 따른 MAE는 유의적인 차이가 있는 지를 분석하였다. 그리고 희소성 문제로 인한 집단 간의 예측 정확도를 높이기 위한 방법으로 희소성이 있는 아이템을 선별하여 이들 중에서 선호도 응답이 많은 사용자 고객의 선호도 평균값을 선호도 평가 치로 대치시켜 희소성을 완화하여 추천시스템의 예측 정확도가 높아졌음을 연구하였다.

• 한국식품저장유통학회지
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• 제7권3호
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• pp.267-272
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• 2000

건고추로부터 올레오레진을 신소가게 추출하고자 기존 추출방법들에 비하여 신속성과 환경친화성이 인정되고 있는 극초단파 추출공정에 필요한 몇 가지 기본조건을 검토하였다. 극초단파 추출에서 건고추의 입자 크기는 60 mesh, 시료와 용매(에탄올)의 비는 1:10(g/mL)이 적당하였고, 에너지 용량은 50 W에서 올레오레진과 capsanthin에 대한 추출효율이 가장 높게 나타났다. 가용성 고형분은 에탄올농도가 낮을수록 증가하였으나 capsanthin 함량은 75% 이상의 에탄올 농도에서 높은 증가현상을 나타내었다. 극초단파에 의한 올레오레진과 capsanthin의 추출은 짧은 시간에 이루어지므로 1회 추출시간은 3~5분이 적당한 것으로 나타났다.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2000년도 춘계학술대회 학술발표 논문집
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• pp.249-255
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• 2000

HRIV(Hybrid Rule-Interval Variation) method is presented to stabilize a class of nonlinear systems, where SMC(Sliding Mode Control) and ADC (ADaptive Control) schemes are incorporated to overcome the unstable characteristics of a conventional FLC(Fuzzy Logic Control). HRIV method consists of two modes: I-mode (Integral Sliding Mode PLC) and R-mode(RIV method). In I-mode, SMC is used to compensate for MAE(Minimum Approximation Error) caused by the heuristic characteristics of FLC. In R-mode, RIV method reduces interval lengths of rules as states converge to an equilibrium point, which makes the defined Lyapunov function candidate negative semi-definite without considering MAE, and the new uncertain parameters generated in R-mode are compensated by SMC. In RIV method, the overcontraction problem that the states are out of a rule-table can happen by the excessive reduction of rule intervals, which is solved with a dynamic modification of rule-intervals and a transition to I-mode. Especially, HRIV method has advantages to use the analytic upper bound of MAE and to reduce Its effect in the control input, compared with the previous researches. Finally, the proposed method is applied to stabilize a simple nonlinear system and a modified inverted pendulum system in simulation experiments.

• Food Science and Biotechnology
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• 제16권1호
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• pp.150-153
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• 2007

Hydrocarbons have been successfully used as a chemical marker in order to identify irradiated from non-irradiated foods. The method for determining hydrocarbons consists of extraction of fats, followed by separation of hydrocarbons by florisil column chromatography, and then identification of hydrocarbons by GC/MS. Currently, solvent extraction method for fats has certain limitations with regard to extraction time and solvent consumption. Commercial hams and sausage were irradiated at 0 and 5 kGy, and the efficiency of microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods on the extraction of radiation-induced hydrocarbons from the meat products was compared. Significant levels of hydrocarbons, mainly composed of 1,7-hexadecadien, 1,7,10-hexadecatriene, and 6,9-heptadecadiene, were detected in the extracts from irradiated hams and sausages by both CSE and MAE methods. Both methods were acceptable in extracting hydrocarbons from samples, but MAE method required apparently reduced amounts of solvent from 150 (CSE) to 50 mL and reduced extraction time from 23 (CSE) to 5 min.

• 한국식품저장유통학회지
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• 제11권1호
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• pp.88-93
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• 2004

마이크로웨이브 추출방법과 환류 냉각 추출방법을 비교한 결과, 물과 에탄올의 혼합용매로 추출한 경우 마이크로웨이브 추출 방법에 의하여 추출시간을 단축시키면서 환류 냉각 추출 방법에서와 같은 수준의 가용성 고형분 및 총 폴리페놀 함량을 갖는 참취 추출물을 얻을 수 있었다. 마이크로웨이브 추출 시 최적 마이크로웨이브 에너지는 120∼150 W였고 추출시간은 4∼8분이 적당하였다. 추출에 사용한 용매들 가운데 에탄올, 메탄을 보다 물 그리고 물과 에탄을 또는 메탄을 혼합용매를 사용한 추출물의 가용성 고형분, 총 폴리페놀 함량 및 항산화 효과가 높은 것으로 나타났다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권5호
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• pp.1690-1707
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• 2021

Precipitation prediction during flood season has been a key task of climate prediction for a long time. This type of prediction is linked with the national economy and people's livelihood, and is also one of the difficult problems in climatology. At present, there are some precipitation forecast models for the flood season, but there are also some deviations from these models, which makes it difficult to forecast accurately. In this paper, based on the measured precipitation data from the flood season from 1993 to 2019 and the precipitation return data of CWRF, ANN cycle modeling and a weighted integration method is used to correct the CWRF used in today's operational systems. The MAE and TCC of the precipitation forecast in the flood season are used to check the prediction performance of the proposed algorithm model. The results demonstrate a good correction effect for the proposed algorithm. In particular, the MAE error of the new algorithm is reduced by about 50%, while the time correlation TCC is improved by about 40%. Therefore, both the generalization of the correction results and the prediction performance are improved.