• Journal of Embryo Transfer
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• v.33 no.4
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• pp.327-336
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• 2018

To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.

• The Journal of the Korea Contents Association
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• v.10 no.1
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• pp.317-324
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• 2010

The purpose of this study was to examine the validity of the Manual Ability Classification System (MACS) by analysing of relation between MACS and Jebsen-Talyor Hand Function Test. The concurrent validity was examined by calculation of correlation between MACS and Jabsen-Talyor Hand Function test and the discriminant validity was examined by measurement of hand function difference according to MACS level. For this, eighty-one children with spastic cerebral palsy were employed in this study. The children were evaluated by using the MACS and Jebsen-Taylor Hand Function Test for their hand function. There were a significant correlation between the MACS and Hand function (r = .870, p < .05). The good correlation between the MACS and subtest of Jabsen-Talyor Hand function (p < .05). The hand function according to the MACS level were different significantly (p < .05). The MACS is valid classification system for assessment of hand function of children with cerebral palsy. The MACS in practice will provide usefulness for assessment of hand function in children with spastic cerebral palsy.

• Journal of Advanced Navigation Technology
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• v.16 no.2
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• pp.255-263
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• 2012

In this paper, the performance evaluation results of the timing synchronization schemes are presented for aeronautical mobile airport communication systems (AeroMACS). AeroMACS, which is based on IEEE 802.16e mobile WiMAX standard, uses the aeronautical frequency band of 5GHz with the bandwidth of 5MHz. The simulation model of AeroMACS is constructed and the evaluation for the timing synchronization performance is performed with the various channel models such as apron (APR), runway (RWY), taxiway (TWY), and park (PRK).

• Journal of Advanced Navigation Technology
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• v.18 no.2
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• pp.142-150
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• 2014

In this paper, the performance analysis results of time/frequency synchronization and cell search algorithm are presented for aeronautical mobile airport communication systems (AeroMACS). AeroMACS is based on IEEE 802.16e mobile WiMAX standard and uses the aeronautical frequency band of 5GHz with the bandwidth of 5MHz. The simulation model of AeroMACS is designed and the performance evaluation is conducted with the various airport channel models such as apron (APR), runway (RWY), taxiway (TWY), and park (PRK). The proposed synchronization unit was designed in hardware description language (HDL) and implemented on FPGA. Also, the real-time operation was verified and evaluated using FPGA test system.

• Journal of Advanced Navigation Technology
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• v.23 no.5
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• pp.373-379
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• 2019

AeroMACS spectrum is a national resource internationally allocated by ITU at WRC-07. AeroMACS is an airport broadband mobile communication infrastructure based on WiMAX-based IEEE 802.16e that enables real-time video, graphics, voice, and high-speed data transmission. With the approval of ICAO's development technology standards in 2008, 50 airports in 11 countries have already completed the testing of D-TAXI or A-SMGCS technology using the AeroMACS infrastructure in 2019, starting in the United States in 2009. With many advantages in safety and convenience in terrestrial telecommunications operations, the system is becoming an area of performance improvement for airport operations in accordance with ICAO's ASBU plan. This paper examines the current status of domestic development of AeroMACS and lists service areas applicable to airlines and operators. It also seeks to promote safe and efficient next-generation airport mobile communication system services by presenting feasible partners management in the mobile area and use of aircraft communication systems for active technology development.

• Physical Therapy Korea
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• v.17 no.1
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• pp.62-68
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• 2010

The purposes of this study were to examine inter-rater reliability of the Manual Ability Classification System (MACS) by children's age and to identify the correlation between the MACS and the Gross Motor Function Classification System (GMFCS) in children with cerebral palsy (CP). Twenty-six children with CP older than two years participated. Children with CP were classified according to the MACS and the GMFCS by two physical therapists. Inter-rater reliability was analyzed using the Intraclass Correlation Coefficients (ICCs). The results showed that the reliability of the MACS for children aged 2~3 years was .88 and for children aged above 4 years was .98 (p<.05). Children with quadriplegia had a higher level of MACS than children with spastic hemiplegia and diplegia. A moderate relationship between the MACS and the GMFCS was found in all children (rater 1, r=.631; rater 2, r=.438). The MACS will be used for classification of children with CP according to the manual abilities. Thus, it offers a reliable method for communicating between therapists about the manual ability of children with CP who are older than 2 years.

• PNF and Movement
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• v.7 no.2
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• pp.21-26
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• 2009

Purpose : The aim of this study was to determine the reliability of parents and therapists-assessed manual ability using the Manual Ability Classification System (MACS) for children with cerebral palsy Methods : Subjects were 136 children with cerebral palsy using rehabilitation programs in 16 welfare centers. Reliability was determined using the intraclass correlation coefficient (ICC). Parents and therapists assessed manual ability of children using MACS. Result : The 136 children (Male 73, Female 63) mean age was 7y 5mo years [range 3y 11mo - 13y 5mo]. The overall agreement between parents-assessed and therapists- assessed MACS was good (ICC = 0.84, 95% confidence interval 0.77-0.88). Conclusion : The MACS offers a reliable method for population-based research and communicating about the manual ability of children with CP.

• The Journal of Korean Academy of Sensory Integration
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• v.10 no.1
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• pp.11-20
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• 2012

Objective : The purpose of this study is intended to provide useful information about the disability evaluation indicators, the Modified Barthel Index (MBI) as a measurement tool for assessing the ability to perform activities of daily living through the correlation between the Modified Barthel Index (MBI) and the Manual Ability Classification System (MACS), and Gross Motor Function Classification System (GMFCS). Methods : The subject was 82 children with cerebral palsy. The validity was aexamined by calculation of correlation between the Modified Barthel Index (MBI), the Manual Ability Classification System (MACS) and Gross Motor Function Classification System (GMFCS). Results : There were a good significant correlation between the MBI and MACS (r = -.765, p <0.001), the MBI and the GMFCS (r = -.851, p < 0.001) and the MACS and the GMFCS (r = .615, p <0.001). Conclusion : The close correlation between the MBI that is used as the basis of grading cerebral palsy and high reliability and validity of the MACS, GMFCS suggest that disability evaluation indicators, the MBI could be useful for children with disabilities.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.3
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• pp.171-178
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• 2006

Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

• Clinical and Experimental Reproductive Medicine
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• v.43 no.4
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• pp.199-206
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• 2016

Objective: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. Methods: Semen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples. Sperm with progressive motility or non-apoptosis were isolated by DGC or MACS, respectively, in 29 normozoospermic semen samples. The effects of DGC or MACS alone and of DGC and MACS combined on reducing the amount of sperm in the sample with DNA fragmentation and protamine deficiency were investigated. Results: The sperm DFI showed a significant correlation (r=-0.347, p< 0.001) with sperm motility and morphology (r=-0.114, p< 0.05) but not with other semen parameters. The DFI ($11.5%{\pm}2.0%$) of semen samples was significantly reduced by DGC ($8.1%{\pm}4.1%$) or MACS alone ($7.4%{\pm}3.9%$) (p< 0.05). The DFI was significantly further reduced by a combination of DGC and MACS ($4.1%{\pm}1.3%$, p< 0.05). Moreover, the combination of DGC and MACS ($1.6%{\pm}1.1%$, p< 0.05) significantly reduced the protamine deficiency rate of semen samples compared to DGC ($4.4%{\pm}3.2%$) or MACS alone ($3.4%{\pm}2.2%$). Conclusion: The combination of DGC and MACS may be an effective method to isolate high-quality sperm with progressive motility, non-apoptosis, high DNA integrity, and low protamine deficiency in clinical use.