• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.83-83
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• 2002

This study was to compare the characteristics of parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. Mouse oocytes were recovered from superovulated 4wks hybrid F1 (C57BL/6xCBA/N) female mice. The oocytes were treated with 7% ethanol for 5 min and 5 ㎍/㎖ cytochalasin-B for 4 h. For IVF, the oocytes were inseminated with epididymal sperm of hybrid Fl male mice (1×10/sup 6//㎖). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count in blastocysts was carried out differential labelling using propidium iodide (red) and bisbenzimide(blue). (omitted)

• Mycobiology
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• v.42 no.4
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• pp.385-390
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• 2014

The beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. Several pest control agents are used to control the beet armyworm. Entomopathogenic fungi are one of the candidates for eco-friendly pest control instead of chemical control agents. In this study, among various entomopathogenic fungal strains isolated from soil two isolates were selected as high virulence pathogens against larva of beet armyworm. Control efficacy of fungal conidia was influenced by conidia concentration, temperature, and relative humidity (RH). The isolates Metarhizium anisopliae FT83 showed 100% cumulative mortality against second instar larvae of S. exigua 3 days after treatment at $1{\times}10^7$ conidia/mL and Paecilomyces fumosoroseus FG340 caused 100% mortality 6 days after treatment at $1{\times}10^4$ conidia/mL. Both M. anisopliae FT83 and P. fumosoroseus FG340 effectively controlled the moth at $20{\sim}30^{\circ}C$. M. anisopliae FT83 was significantly affected mortality by RH: mortality was 86.7% at 85% RH and 13.4% at 45% RH. P. fumosoroseus FG340 showed high mortality as 90% at 45% RH and 100% at 75% RH 6 days after conidia treatments. These results suggest that P. fumosoroseus FG340 and M. anisopliae FT83 have high potential to develop as a biocontrol agent against the beet armyworm.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.10a
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• pp.985-988
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• 2014

This study was conducted to investigate antioxidative activities of ethanol extracts from Erigeron canadensis L. The ethanol extracts were measured to examine total flavonoids and polyphenol content, DPPH radical activity and SOD-like activity. Total flavonoids content in leaf extract (42.19 mg/g) and total polyphenol content in flower extract (74.26 mg/g) were higher than those of other parts. All assays were conducted at concentrations of 0.1, 0.3, 0.5, and 1 mg/mL ethanol extracts. The DPPH radical activity of leaf, flower, the whole plant, and root extracts were 91.75%, 86.95%, 83.95% and 83.43%, respectively, at a concentration of 1 mg/mL. The activities were concentration dependent. The SOD-like activity of ethanol extracts from different parts was 7.91~13.54% at a concentration of 1 mg/mL. These results suggest that ethanol extracts from different parts of Erigeron canadensis L. could be used as antioxidative functional food sources.

• BMB Reports
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• v.39 no.1
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• pp.22-25
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• 2006

The gene encoding MPB83 from Mycobacterium bovis Vallee111 chromosomal DNA was amplified by using polymerase chain reaction (PCR) technique, and the PCR product was approximately 600bp DNA segment. Using T-A cloning technique, the PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-83 was constructed successfully. pGEM-T-83 and pET28a(+) were digested by BamHI and EcoRI double enzymes. The purified MPB83 gene was subcloned into the expression vector pET28a(+), and the prokaryotic expression vector pET28a-83 was constructed. Plasmid containing pET28a-83 was transformed into competence Escherichia coli BL21 (DE3). The bacterium was induced by isopropyl-$\beta$-D-thiogalactopyranoside (IPTG) and its lysates were loaded directly onto sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), approximately 26 kDa exogenous protein was observed on the SDS-PAGE. The protein was analyzed using Western-blotting. The results indicated that the protein was of antigenic activity of M. bovis. The results were expected to lay foundation for further studies on the subunit vaccine and DNA vaccine of MPB83 gene in their prevention against bovine tuberculosis.

• 대한핵의학회:학술대회논문집
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• 1970.10a
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• pp.83.1-83.1
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• 1970

• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.83-83
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• 2002

• 대한핵의학회:학술대회논문집
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• 1971.11a
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• pp.83.1-83.1
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• 1971

• The Bulletin of The Korean Astronomical Society
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• v.36 no.1
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• pp.83.2-83.2
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• 2011

I present results from the Spitzer/IRS study to identify massive young stellar objects (YSOs) in the Galactic Center (GC). Our sample of 107 YSO candidates was selected based on Spitzer/IRAC colors in the central 300 pc region of the Milky Way Galaxy. We obtained IRS spectra over $5{\mu}m$ to $35{\mu}m$, and identified massive YSOs by the presence of a $15.4{\mu}m$ shoulder on the absorption profile of $15{\mu}m$ $CO_2$ ice, suggestive of high $CH_3OH$ abundance on $CO_2$ ice grains. This $15.4{\mu}m$ shoulder is clearly observed in 16 sources and possibly observed in an additional 19 sources. We further show that 9 massive YSOs reveal molecular gas-phase absorption from $CO_2$, $C_2H_2$, and/or HCN, which traces warm and dense gas in YSOs. Our results provide the first spectroscopic census of the massive YSO population in the GC.

• BMB Reports
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• v.46 no.9
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• pp.448-453
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• 2013

CpG-DNA has various immunomodulatory effects in dendritic cells, B cells, and macrophages. While induction of cytokines by CpG-DNA has been well documented in macrophages, the expression of costimulatory molecules in CpG-DNA treated macrophages has not yet been defined. Therefore, we investigated the effects of CpG-DNA on the expression of costimulatory molecules in RAW 264.7 cells. The surface expression of CD80 was slightly increased and CD83 expression was significantly increased in response to CpG-DNA. However, the expression of CD86 and MHC class II was not changed. As expression of CD83 mRNA was also increased by CpG-DNA, CD83 expression is regulated at a transcriptional level. To understand the contribution of signaling pathways to CD83 induction, we used pathway specific inhibitors. The NF-${\kappa}B$ inhibitor significantly reduced surface expression of CD83 as well as phagocytic activity of RAW 264.7 cells. Therefore, CD83 expression may contribute to the immunostimulatory effects of CpG-DNA in macrophage cells.

• Kyungpook Mathematical Journal
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• v.14 no.1
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• pp.83-84
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• 1974