• Title/Summary/Keyword: M83

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PHYSICS REVEALED BY BROAD-RANGE CO LADDERS AND FINE-STRUCTURE LINES IN M83

  • Wu, Ronin
    • Publications of The Korean Astronomical Society
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    • v.32 no.1
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    • pp.147-149
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    • 2017
  • Since the launch of the Herschel Space Observatory, our understanding about the photo-dissociation regions (PDR) has taken a step forward. In the bandwidth of the Fourier Transform Spectrometer (FTS) of the Spectral and Photometric Imaging REceiver (SPIRE) on board Herschel, ten CO rotational transitions, including J = 4 - 3 to J = 13 - 12, and three fine structure lines, including [$C{\small{I}}$] 609, [$C{\small{I}}$] 370, and [$N{\small{II}}$] $205{\mu}m$, are covered. I present our findings from the FTS observations at the nuclear region of M83, based on the spatially resolved physical parameters derived from the CO spectral line energy distribution (SLED) map and the comparisons with the dust properties and star-formation tracers. This article discusses (1) the potential of using [$N{\small{II}$] 205 and [$C{\small{I}}$] $370{\mu}m$ as star-formation tracers; (2) the excitation mechanisms of warm CO in the nuclear region of M83.

A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis

  • Song, Shengnan;Zhang, Qian;Yang, Hang;Guo, Jia;Xu, Mingguo;Yang, Ningning;Yi, Jihai;Wang, Zhen;Chen, Chuangfu
    • Journal of Veterinary Science
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    • v.23 no.3
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    • pp.50.1-50.12
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    • 2022
  • Background: There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity. Objectives: To develop a reliable and rapid strategy to improve diagnostic tools for bTB. Methods: In this study, we expressed and purified the sixteen bTB-specific recombinant proteins in Escherichia coli. The two antigenic proteins, MPT70 and MPT83, which were most valuable for serological diagnosis of bTB were screened. Molecular docking technology was used to analyze the affinity of MPT70, MPT83, dominant epitope peptide of MPT70 (M1), and dominant epitope peptide MPT83 (M2) with TLR2, combined with the detection results of enzyme-linked immunosorbent assay to evaluate the molecular docking effect. Results: The results showed that interaction surface Cα-atom root mean square deviation of proteins (M1, M2, MPT70, MPT83)-TLR2 protein are less than 2.5 A, showing a high affinity. It is verified by clinical serum samples that MPT70, MPT83, MPT70-MPT83 showed good diagnostic potential for the detection of anti-bTB IgG and M1, M2 can replace the whole protein as the detection antigen. Conclusions: Molecular docking to evaluate the affinity of bTB protein and TLR2 combined with ELISA provides new insights for the diagnosis of bTB.

Monitoring the Manufacturing Characteristics of Aloe Gel-State Food (알로에 겔상 식품의 제조특성 모니터링)

  • 이기동;김숙경;권도영;박상렬
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.89-95
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    • 2003
  • Four-dimensional response surface methodology was used for monitoring the manufacturing characteristics of aloe gel-state food. The optimum conditions predicted for each corresponding sensory properties of aloe gel-state food were 87.38 mL (content of aloe juice), 0.16 g (content of konjac) and 0.19 g (content of carrageenan) in coloror of gel-state food, 83.84 mL, 0.17 g and 0.20 g in aroma of aloe gel-state food, 83.20 mL.0.15 g and 0.27 g taste of aloe gel-state food and 98.95 mL, 0.10 g and 0.23 g in texture of aloe gel-state food. Maximum chewiness of aloe gel-state food was in 113.05 mL aloe juice, 0.27 g konjac and 0.21 g carrageenan. The optimum conditions, which satisfied all sensory properties of gel-state food, were 88.23 mL, 0.15 g and 0.49 g in content of aloe juice, content of konjac and content of carrageenan, respectively.

Application of Temperature Gradient Gel Electrophoresis To cAMP Receptor Protein (온도 기울기 전기영동장치의 CAMP 수용성 단백질에 응용)

  • Gang, Jong-Back;Cho, Hyun-Young
    • Journal of Life Science
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    • v.14 no.2
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    • pp.309-314
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    • 2004
  • Cyclic AMP receptor protein (CRP) is involved in the transcriptional regulation of more than 100 genes in E. coli. CRP dimer is converted into active form via the sequential conformation change of cAMP binding pocket, hinge region and HTH DNA binding motif by binding of cAMP. The temperature gradient gel electrophoresis (TGGE) was applied to CRP protein to know whether it was an efficient technique to study the conformational transitions and the thermal stability. TGGE showed the unfolding process of wild-type and S83G CRP proteins with the temperature gradient set from 29 to 71$^{\circ}C$ on nondenaturing polyacrylamide gel. Melting temperature (Tm) was 57$\pm$1 and 55$\pm$1$^{\circ}C$ for wild-type and S83G CRP, respectively in acidic buffer[89.8 mM Glycine and 24 mM Boric acid (pH 5.8)].

An Satellite Communication Wireless Package System Using Analysis of Channel Interference between ISM band Systems (ISM 대역 시스템간 채널 간섭 분석을 통한 위성 통신 무선 패키지시스템 적용)

  • Ko, Hojeong;Cha, Jaesang
    • Journal of Satellite, Information and Communications
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    • v.10 no.3
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    • pp.78-83
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    • 2015
  • In this paper, when WLAN repeater of satellite communication package system as a novel wireless disaster communication network connected to LTE D2D mobile terminal, we analyzed radio channel interference from WLAN and WPAN system of adjacent same ISM band using Monte-Carlo method. In this study, WLAN cell radius was determined using Extended Hata Model considering practical environment, and simulated physical protection distance and density in the dense-mode to minimize interference from WLAN, Bluetooth, and ZigBee. Simulation results, WLAN repeater can be operated with 15 WLAN interferer over 130m distance, 23 Bluetooth interferer over 100m distance, and with 62 ZigBee interferer over 83m distance.

Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum (Corynebacterium lactofermentum에서 Bacillus subtilis의 Mannanase 유전자 발현)

  • Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.405-407
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    • 2009
  • A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli- Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.

Insecticidal Activity of Metarhizium anisopliae FT83 against the Different Stages of Beet Armyworm, Spodoptera exigua (곤충병원성 곰팡이 Metarhizium anisopliae FT83의 파밤나방 생육단계별 살충활성)

  • Han, Ji Hee;Kim, Jeong Jun;Lee, SangYeob
    • The Korean Journal of Pesticide Science
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    • v.18 no.4
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    • pp.417-421
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    • 2014
  • The beet armyworm, Spodoptera exigua is pest which is difficult to control. For eco-friendly beet armyworm managements, we isolated entomopathogenic fungi from soil samples by insect-bait method using Tenebrio molitor and conducted bioassay to larvae of beet armyworm. The result of bioassay, a selected strain Metarhizium anisopliae FT83 caused 100% mortality against first ~ third instar larva of S. exigua at $1{\times}10^8conidia/ml$ and medial lethal time ($LT_{50}$) were 0.5 days, 2.6 days and 2.5 days respectively. Mortality against fourth and fifth larvae were $83.3{\pm}6.2%$ and $86.0{\pm}5.7%$ and medial lethal time ($LT_{50}$) were 4.2 days and 3.6 days respectively. Mortality against pupae and eggs of S. exigua were 100%. M. anisopliae FT83 showed high virulence at all developmental stages of S. exigua.

Semi M-Projective and Semi N-Injective Modules

  • Hakmi, Hamza
    • Kyungpook Mathematical Journal
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    • v.56 no.1
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    • pp.83-94
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    • 2016
  • Let M and N be modules over a ring R. The purpose of this paper is to study modules M, N for which the bi-module [M, N] is regular or pi. It is proved that the bi-module [M, N] is regular if and only if a module N is semi M-projective and $Im({\alpha}){\subseteq}^{\oplus}N$ for all ${\alpha}{\in}[M,N]$, if and only if a module M is semi N-injective and $Ker({\alpha}){\subseteq}^{\oplus}N$ for all ${\alpha}{\in}[M,N]$. Also, it is proved that the bi-module [M, N] is pi if and only if a module N is direct M-projective and for any ${\alpha}{\in}[M,N]$ there exists ${\beta}{\in}[M,N]$ such that $Im({\alpha}{\beta}){\subseteq}^{\oplus}N$, if and only if a module M is direct N-injective and for any ${\alpha}{\in}[M,N]$ there exists ${\beta}{\in}[M,N]$ such that $Ker({\beta}{\alpha}){\subseteq}^{\oplus}M$. The relationship between the Jacobson radical and the (co)singular ideal of [M, N] is described.