• Title/Summary/Keyword: M50

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Antioxidative Activities and Inhibitory Effects on Tyrosinase and Elastase by Water Extracts of Allium sp. (Allium 속 식물 물 추출물의 항산화 활성과 Tyrosinase 및 Elastase 저해 효과)

  • Jia, Yuan;Yang, Ming;Park, Inshik
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.247-252
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    • 2015
  • Three different Allium plants, including wild garlic (Allium monanthum Max; AMM), onion (Allium cepa L.; ACL) and green onion (Allium fistulosum L; AFL), have been widely used as spicy food in daily life. This study was attempted to investigate the properties of antioxidant, whitening and anti-wrinkle activities in water extracts in Allium plants. The antioxidant, whitening and anti-Wrinkle activities were evaluated by performing DPPH and ABTS radical scavenging activities, inhibitory effects on tyrosinase, and elastase, respectively. The water extract (10 mg/mL) of onion, wild garlic and green onion exhibited DPPH radical scavenging activities of 28.46%, 28.45%, and 15.91%, respectively. The inhibitory effects of tyrosinase activities by wild garlic, green onion, and onion were increased by heating the extracts at $90^{\circ}C$. Additionally, the wild garlic extract showed higher elastase inhibitory activity than those of other plant extracts. These results suggest that water extracts of Allium plants may be useful as potential sources of beauty foods due to higher antioxidant, anti-melanin and anti-wrinkle activities.

In vitro Screening of Jeju Medicinal Plants for Cosmeceutical Materials

  • Kim, Sang-Suk;Hyun, Chang-Gu;Lee, Jong-Sung;Lim, Ji-Hee;Kim, Ji-Young;Park, Deok-Hoon
    • Journal of Applied Biological Chemistry
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    • v.50 no.4
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    • pp.215-220
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    • 2007
  • One of the important functions of skin is protection from harmful environments. Many studies have explored how to prevent skin from wrinkling and the occurrence of pigmentation changes. Skin wrinkling and pigmentation changes could be caused by unusual disruption of connective tissue, the formation of free radicals and ultraviolet radiation. In this study, extracts obtained from 254 different kinds of Jeju medicinal plants were screened for inhibitory effects on tyrosinase and elastase, and for free radical scavenging effects. Four herbs, Phormium tenax, Morus bombycis, Morus alba, and Cudrania tricuspidata, were potent inhibitors of tyrosinase ($IC_{50}$ values 4.62, 5.46, 8.17, and 64.17 ${\mu}g$/mL, respectively). Aleurites fordii [$IC_{50}$: 5.29 ${\mu}g$/mL, 1,1-diphenyl-2-picrylhydrazyl (DPPH)], Distylium racemosum ($IC_{50}$: 6.14 ${\mu}g$/mL), Acer palmatum ($IC_{50}$: 5.44 ${\mu}g$/mL), and Spiraea salicifolia ($IC_{50}$: 5.25 ${\mu}g$/mL) showed good antioxidative effects. Furthermore, Distylium racemosum ($IC_{50}$: 7.51 ${\mu}g$/mL), Diospyros kaki ($IC_{50}$: 15.1 ${\mu}g$/mL), Cornus macrophylla ($IC_{50}:$ 16.59 ${\mu}g$/mL), and Psidium guajava ($IC_{50}$: 40.25 ${\mu}g$/mL) exhibited potent inhibitory effects on elastase. These results suggest that medicinal plants possessing several biological activities may be potent inhibitors of the processes involved in pigmentation increases and aging. Further investigations will focus on in vivo assays and on the chemical identification of the major active components responsible for whitening and anti-aging activity in the screened efficacious extracts.

Evaluation of Antioxidant Potential of Extract/Fractions of Equisetum arvense (I) (쇠뜨기 추출물의 항산화 작용에 관한 연구(I))

  • Park, Soo-Nam;Yang, Hee-Jung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.2
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    • pp.61-67
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    • 2007
  • In this study, the antioxidative effects of Equisetum arvense extracts were investigated. The free radical (1,1 diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$) of extract/fractions of Equisetum arvense was in the order: 50 % ethanol extract ($182.04{\mu}g/mL$) < ethylacetate fraction ($54.50{\mu}g/mL$) < deglycosylated flavonoid aglycone fraction ($14.13{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some Equisetum arvense extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol- dependent chemiluminescence assay. The order of ROS scavenging activity was deglycosylated flavonoid aglycone fraction ($OSC_{50}$, $3.54{\mu}g/mL$) < 50 % ethanol extract ($0.80{\mu}g/mL$) < ethylacetate fraction ($0.006{\mu}g/mL$). Ethylacetate fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Equisetum arvense on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethanol extract (50%) suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated aglycone extract exhibited the most prominent celluar protective effect ($\tau_{50}$, 161.10 min at $10{\mu}g/mL$). These results indicate that extract/fractions of Equisetum arvense can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS.

A Study on the Lift Pier and Equipment of Design Criteria for Effective Vessel Management in the Marina (마리나 항만의 효율적인 선박 관리를 위한 상하가 시설 및 장비의 설계기준에 관한 연구)

  • Moon, Jung-Hwan
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.6
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    • pp.654-661
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    • 2018
  • This paper analyzes the type of facilities and equipment of the marina port and analyzes its lift facilities (lift pier) and equipment (marine mobile lift). It set up the lift facilities and equipment standards. Depending on the gross tonnage of vessels in operation in Korea, the required inner width of lift piers is 5.50 m for 35-ton vessels, and 6.20 m for 50-ton vessels. The inside width of the marine mobile lift is 6.10 m for 35-ton vessels, and 6.80 m for 50-ton vessels. There are two places where the target ship can be lifted from the lift pier installed on the domestic marina, and other marinas' lift piers inner widths are insufficient by about 0.35 ~ 0.50 m. Among the marine mobile lifts in operation, there were two apparatuses that secured the inside width necessary to lift the target vessel. Other marine mobile lifts' inner widths are insufficient by about 0.3 ~ 0.6 m.

Varietal Difference of Salt Tolerance during Germination in Italian Ryegrass (이탈리안라이그래스 발아기 내염성의 품종간 차이)

  • 이강수;최선영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.4
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    • pp.413-419
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    • 1995
  • This study was conducted to find out the suitable NaCl concentration and temperature for investigating the varietal difference of salinity tolerance in Italian ryegrass. Seeds of 20 cultivars including 9 diploid and 11 tetraploid were exposed to eight levels of NaCl concentration ranging from 0 to 350mM under three temperature levels of 15, 20 and $25^{\circ}C$. The NaCl concentration(C50%) inhibited germination of 50% of the viable seeds for the cultivars at 15$^{\circ}C$ was 354mM, and those at 2$0^{\circ}C$ and $25^{\circ}C$ were 342mM and 325mM, respectively. There were significant correlations among C50% at three levels of temperature, and correlation coefficient for C50% between 2$0^{\circ}C$ and 15$^{\circ}C$ was higher than that between $25^{\circ}C$ and 15$^{\circ}C$ or 2$0^{\circ}C$. C50% was positively correlated only with germination speed at 10 days after sowing when the plants were exposed to 350mM NaCl at 2$0^{\circ}C$. For the germination speed at 10 days after sowing in 350mM NaCl at 2$0^{\circ}C$, the cultivars used could be classified into three groups. Wase Aoba, Bettina, E. K -11, Tetrone, Lira Sand, Tetra Florum and Billiken belonged to the tolerant group, and Tuchi Was, Wase Yutaka, Sakura Wase, Magnolia, Limella and Delita were as the semi-susceptible group, and Atalja, Barmultra, Ajax, Liberta, LM-16, Elving and Wilo were as the susceptible group.

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VARIABLE STARS IN THE REGION OF AN OPEN CLUSTER M50 (산개성단 M50 영역의 변광성)

  • Jeon, Young-Beom
    • Publications of The Korean Astronomical Society
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    • v.23 no.2
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    • pp.17-23
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    • 2008
  • From the short-period variability survey (SPVS), we obtained time-series BV CCD images in the region of an open cluster M50 (NGC 2323) for 10 nights from 2008 February 9 to 27. The observation was performed using a small refracting telescope (${\phi}$=155mm, f=1050mm) in Bohyunsan Optical Astronomy Observatory (BOAO). M50 is included in the observing field of ASAS (The All Sky Automated Survey). In the region of M50, we found 8 new variable stars including two ${\delta}$ Scuti stars with short-periods and very small amplitudes. There are known nine variable stars by the catalogues of ASAS and GCVS in the region. We confirmed the variability of the known variable stars for six and non-variability for three.

Comparison of antioxidant activities of ethanol extracts from seven species of wild edible plants in Korea (국내 야생식용식물 7종 에탄올 추출물의 항산화 활성 비교)

  • Ji, Hee Young;Joo, Shin Youn
    • Korean Journal of Food Science and Technology
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    • v.53 no.5
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    • pp.578-584
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    • 2021
  • In this study, we investigated the potential of 70% ethanol extracts from wild edible plants (Pueraria lobata sprout, Rosa multiflora sprout, Artemisia princeps leaf, Diospyros kaki leaf, Morus alba leaf, Robinia pseudoacacia flower, Inula britannica var. japonica flower), as natural antioxidants. The antioxidant contents and activities of extracts were examined using various methods. The measurements of total polyphenol content revealed that Rosa multiflora sprout extract had the highest value and total flavonoid content showed that Diospyros kaki leaf extract had the highest value. Antioxidant activities were the highest in Rosa multiflora sprout for DPPH (IC50 232.52 ㎍/mL), ABTS+ (IC50 470.10 ㎍/mL), superoxide- (IC50 431.88 ㎍/mL), nitrite (IC50 363.38 ㎍/mL) scavenging activity, and reducing power (2.47 O.D.). These results suggest that the ethanolic extract of Rosa multiflora sprout is a potential source of natural antioxidants.

Antiproliferative Effect and Apoptotic Induction of Bauhinia forficata Extract in Human Cancer Cells. (Bauhinia forficata 추출물의 인체 암세포에 대한 성장억제 및 세포사멸 유도 활성)

  • 임혜영;이철훈
    • Microbiology and Biotechnology Letters
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    • v.32 no.1
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    • pp.67-71
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    • 2004
  • Pata de Vaca (Bauhinia forficata) Is a tree which grows naturally in the rainforests and tropical parts of Peru and Brazil, as well as tropical zones of Asia, eastern Paraguay and northeastern Argentina. The active fraction (Pata-50) of the 70% ethanol extract from Pata de Vaca was sequentially fractionated by HP-20 Diaion column chromatography and C-18 column chromatography, and its characteristics were investigated. The growth of all cancer cells tested except for MCF-7 was Inhibited in a concentration-dependent manner by Pata-50. Its $IC_{50}$ values were estimated to be 40.4 $\mu\textrm{g}$/$m\ell$ on AGS, 51.3 $\mu\textrm{g}$/$m\ell$ on HT-29, 52.1$\mu\textrm{g}$/$m\ell$ on HepG2, 65.2$\mu\textrm{g}$/$m\ell$ on A549, and 77.5$\mu\textrm{g}$/$m\ell$ on HeLa cells. A flow cytometric analysis of HepG2 cells revealed induction of apoptosis, but cell cycle regulation was not affected. The HepG2 cell population of apoptosis region increased In a concentration-dependent manner by Pata-50.

Enzymatic Assessment of $2-Hydroxyethyl-{\beta}-undecenate$ Purified from Cumin (Cuminum cymium L.) Seed for Anti-periodontitis (Cumin(Cuminum cymium L.) seed로부터 정제한 $2-hydroxyethyl-{\beta}-undecenate$의 항치주염 효과의 효소학적 평가)

  • Ryu, Il-Hwan;Kang, Eun-Ju;Lee, Kap-Sang;Park, Chung-Soon
    • Korean Journal of Food Science and Technology
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    • v.39 no.6
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    • pp.669-675
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    • 2007
  • The present study was conducted to explore the anti-inflammatory action of $2-hydroxyethyl-{\beta}-undecenate$ (HPS) purified from Cumin (Cuminum cymium L.) seed against periodontitis. From the study in which human leukocyte was employed to detect the inhibiting effects of 5-lipokygenase and cyclooxygenase, enzymes generating carriers of infection like $LTB_4$ and PGs, as well as of collagenase and elastase, organ-destroying enzymes, following conclusions could be drawn: HPS was found to inhibit leukotrien $B_4$ biosynthesis by stimulating more than 97% of human polymorphonuclear leukocyte (PMNL) with addition of $5\;{\times}\;10^{-2}\;M$ when $IC_{50}$ was set at $2\;{\times}\;10^{-4}\;M$. Ninety-two percent of enzyme activation turned out to be inhibited when $5\;{\times}\;10^{-2}\;M$ was added in a test to prove inhibiting effects of HPS against activation of PMNL 5-lipoxygenase from homogeneous humans and purified 5-lipoxygenase on the market. Besides, $IC_{50}$ for enzyme activation was valued at $2.5\;{\times}\;10^{-4}\;M$, while the value of $IC_{50}$ for purified 5-lipoxygenase was $2.3\;{\times}\;10^{-4}\;M$. The $IC_{50}$ values of COX-activated leukocyte and purified collagenase were $5.1\;{\times}\;10^{-4}\;M$ and $2.3\;{\times}\;10^{-4}\;M$, respectively. Moreover, the value of $IC_{50}$ for activation of leukocyte collagenase was $2\;{\times}\;10^{-3}\;M$, whereas that for purified collagenase was $5\;{\times}\;10^{-2}\;M$. In case of leukocyte elastase, addition of $5\;{\times}\;10^{-2}\;M$ inhibited its activation by 66%. In case of purified one, however, activation of enzyme was inhibited by 25% with addition of $5\;{\times}\;10^{-2}\;M$. Furthermore, the $IC_{50}$ value for activation of leukocyte elastase was revealed to be $7.5\;{\times}\;10^{-3}\;M$. From the virulence test with human gingiva cell, it was shown that, on the second day of cultivation, 47.83% of the cell had been activated when HPS was added by $5\;{\times}\;10^{-2}\;M$. Even the addition of HPS by $1\;{\times}\;10^{-2}\;M$ featured 68.53% of cell activation, suggesting relatively strong toxicity of the substance against gingiva cell.

A study on Requirements of the Test Flight Safety for a 350m Unmanned Airship (50m급 무인비행선 시험비행 안전성 요구사항에 관한 연구)

  • Jeong, B.G.;Shin, D.W.
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.12 no.2
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    • pp.1-15
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    • 2004
  • In this study, we analyze technical requirements for a 50m unmanned airship order to assure safety in the test flight operation. The 50m class unmanned airship developed by Airship Group in Korea Aerospace Research Institute(KARI). The 50m class unmanned airship was developed as a scale-down model of 200m class stratosphere unmanned airship. This study reviews specifications and characteristics of 50m class unmanned airship and develops Airworthiness Requirements on the basis of current effective Aviation Act, Airworthiness standards, and relevant international documents. The developed requirements can be applied to the safety assessment of the 200m class stratosphere unmanned airship.

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