• 대한한의학회지
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• 제19권2호
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• pp.450-467
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• 1998

To research the characteristics of ion currents induced by inhibitory and excitatory herbs of oriental medicine, we used nystatin-perforated patch clamp technique under voltage clamp condition in periaqueductal gray neuron dissociated from Sprauge-Dawley rat, 10-15 days old. The results are as follows. 1. Ion current induced by $10mg/m{\ell}$ of Bupleuri Radix was inhibited $59.50{\pm}4.29%$ by $10^{-4}M$ bicuculline(p>0.01) but inhibition of $10.75{\pm}4.77%$ by $10^{-4}M$ tubocurarine and $4.75{\pm}4.23%$ by $10^{-4}M$ verapamil had no statistical significance(p>0.05). So ion current induced by Bupleuri Radix revealed only GABA induced $Cl^-$ current, not acetylcholine and $Ca^{2+}$ current. 2. Ion current induced by $20mg/m{\ell}$ of Coptidis Rhizoma was inhibited $47.20{\pm}7.88%$ by $10^{-4}M$ bicuculline(p<0.01) but $3.20{\pm}2.33%$ inhibition by $10^{-4}M$ tubocurarine and $1.00{\pm}1.00%$ inhibition by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Coptidis Rhizoma revealed only GABA induced $Cl^-$ current, not acetylcholine and $Ca^{2+}$ current. 3. Ion current induced by $20mg/m{\ell}$ of Ecliptae Herba was inhibited $55.00{\pm}4.92%$ by $10^{-4}M$ bicuculline (p<0.01), and also inhibited $15.00{\pm}4.26%$ by $10^{-4}M$ tubocurarine(p<0.05), but inhibition of $6.00{\pm}3.03%$ by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Ecliptae Herba showed GABA activated $Cl^-$ current and acetylcholine activated cation current, not $Ca^{2+}$ current 4. Ion current induced by $5mg/m{\ell}$ of Liriopis Tuber was inhibited $15.20{\pm}4.57%$ by $10^{-4}M$ bicuculline<0.05) and also inhibited $14.00{\pm}3.00%$ by $10^{-4}M$ tubocurarine(p<0.05), but inhibition of $5.20{\pm}4.80%$ by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Liriopis Tuber showed GABA. activated $Cl^-$ current and acetylcholine activated cation current, not $Ca^{2+}$ current. 5. Ion current induced by $5mg/m{\ell}$ of Aconiti Tuber was inhibited $97.00{\pm}1.34%$ by $10^{-4}M$ bicuculline(p<0.01), $80.00{\pm}9.83%$ by $10^{-4}M$ tubocurarine(p<0.01), and $24.00{\pm}6.18%$ by $10^{-4}M$ verapamil(p<0.05). So ion current induced by Aconiti Tuber revealed GABA activated $Cl^-$ current and acetylcholine activated cation current and $Ca^{2+}$ current. 6. Ion current induced by $10mg/m{\ell}$ of Zingiberis Rhizoma was inhibited $33.00{\pm}7.43%$ by $10^{-4}$ bicuculline(p<0.05), $10.20{\pm}1.83%$ by $10-^{-4}M$ tubocurarine(p<0.01), and $14.00{\pm}2.16%$ by $10^{-4}M$ verapamil(p<0.01) So ion current induced by Zingiberis Rhizoma revealed GABA activated $Cl^-$ current and acetylcholine activated cation outtent and $Ca^{2+}$ current. 7. Ion current induced by $10mg/m{\ell}$ of Boshniakiae Herba was inhibited $65.00{\pm}13.75%$ by $10^{-4}M$ bicuculline(p<0.05), $38.00{\pm}9.24%$ by $10^{-4}M$ tubocurarine(p<0.05), and $33.25{\pm}7.42%$ by $10^{-4}M$ verapamiHp<0.05). So ion current induced by Bpshniakiae Herba revealed GABA activated $Cl^-$ current and acetylcholine activated cation current and $Ca^{2+}$ current. These results suggest that a point of difference between inhibitory and excitatory herbs is existence of$Ca^{2+}$ current.

• 생명과학회지
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• 제28권11호
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• pp.1262-1267
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• 2018

국지적 환경에서 패치 분포 형태는 일차적으로 식물의 공간적 양상으로 나타나고, 임의화 과정은 패치를 결정하는 공간적 가설을 입증하는데 이용된다. 주름조개풀(Oplismenus undulatifolius (Ard.) P. Beauv. var. undulatifolius)은 Poaceae과 Oplismenus 속에 속하는 초본류이다. 이 종은 온대, 아열대, 열대에 분포한다. 이 지역의 주름조개풀이 일정한 분포인지 응집하는 분포인지 여러 패치 지표를 사용하여 분석하였다. 한우산의 집단 밀도는 0.453에서 4.375였으며 평균은 2.387이였다. 소형과 중형 프롯($2m{\times}2m$, $2m{\times}4m$, $4m{\times}4m$, $4m{\times}8m$, and $8m{\times}8m$)에서 주름조개풀은 응집형태를 보였다. 반면에 대형 플롯($8m{\times}16m$, $16m{\times}16m$, and $16m{\times}32m$)에서는 일정한 분포 양상을 나타내었다. 평균 응집 계수(M*)와 패치 지표(PAI)는 양의 값을 보였다. 응집은 여러 환경적 요인에 기인하는데 등산객들에 의해 방해되고 있었다. 따라서 주름조개풀은 이 지역에서 본래의 기본 생태적 지위를 누리지 못하고 있음을 보여주었다.

• Journal of Astronomy and Space Sciences
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• 제2권2호
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• pp.101-115
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• 1985

Using Brandt model the mess distribution of the late type spiral galaxy NGC 6946 was derived, and the total mass was reestimated to understand the M/L ratio of this galaxy. Two kinds of the rotation curve with shape parameter n=1 and 3.3 were examined. The following are the main results; (1) The total masses of NGC 6946 are $3.1\times$$10^{11}$ M(n=1) AND $2.8\times$$10^{11}$ M(n=3.3) respectively. and the corresponding M/L are about 17 and 16 for both cases. (2) The optical image in the blue light, whose radius is 9.6 kpc, has 8$\times$$10^{10}$ M and 1.4$\times$$10^{11}$ M. These give the value of M/L about 5 and 8 respectively. (3) The masses and M/L of the nuclear region within 1.2 kpc are 4.0$\times$$10^{9}$ M 4.7$\times$$10^9$ M and 3, 4 for both cases. Those of the disk from 1.2 kpc to 9.6 kpc are 7.6$\times$1$10^{10}$M, 1.4$\times$$10^{11}$M, and 5, 8. (4) The masses of the outer halo extended to few hundreds kiloparsecs are 2.3$\times$$10^{11}$ M and 1.4$\times$$10^{11}$M. The corresponding M/L are about 62 and 37.

• 지구물리
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• 제5권1호
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• pp.41-50
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• 2002

경주시 감산사 분근에서 발견된 제4기 단층 부근의 기반암 분포를 확인하기 위하여 188m 측선의 굴절파 자료를 4m 간격으로 획득하였으며, 토모그래피 역산을 통하여 350 m/s, 600 m/s, 1,100 m/s, 2,400 m/s의 속도를 갖는 4개 층을 구명하였다. 주변지질을 감안하여 속도분포를 분석한 결과, 원점에서 40 m 부근에 역단층이 존재하며 북북서쪽으로 단층파쇄대가 존재하는 것으로 해석된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.192-197
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• 2005

The effects of macronutrients $(NO_3^-,\; NH_4^+\;and\;PO_4^{3-})$ on cell growth and triterpenoids production in Centella asiatica cell suspension cultures were analyzed using the Box­Behnken response surface model experimental design. In screening and optimization experiments, $PO_4^{3-}$ as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated cell growth from 3.9 to $14\~16g/L$. The optimum values predicted from the response surface model are 5.05mM $NH_4^+$, 15.0mM $NO_3^-$ and 2.6mM $PO_4^{3-}$, yielding 16.0g/L cell dry weight with $99\%$ fitness to the experimental data. While the $NH_4^+-NO_3^-$ interaction influenced cell growth positively in the optimization experiment, $NH_4^+$ and $NO_3^-$ as single factors; and interactions of $NO_3^--PO_4^{3-},\;NH_4^+-PO_4^{3-}$ and $NH_4^+-NO_3^-$ were all negative in the screening experiment. Cell growth and the final pH level were positively affected by $PO_4^{3-}$, but negatively affected by $NH_4^+\;and\;NH_4^+-PO_4^{3-}$ interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4mg/g cell dry weight.

• 미생물학회지
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• 제19권1호
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• pp.31-37
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• 1981

Avicelase, CMCase and salicinase of A.phoenicis K.U. 175 were purified from wheat bran culture by salting out with ammonium sulfate, dialysis and successive column chromatography Sephadex G-100. Optimum pH and temperature of avicelase were pH 3.8-4.8, $35-55^{\circ}C$ and that of CMCase, salicinase were pH4.5-5.5, $45-60^{\circ}C$ and pH 4.5-6.0, $45-60^{\circ}C$ respectively. These enzymes were relatively thermostable, alkali unstable and inhibited by $Ca^{++},\;Mn^{++},\;Cu^{++},\;and\;Hg^{++}$. Km values of avicelase, CMCase and salicinase were calculated to be $1.5{\times}10^{-4}M,\;5.5{\times}10^{-4}M\;and\;2.75{\times}10^{-5}M$ and Vmax values $1.66{\times}10^{-4}mM/min,\;3.33{\times}10^{-4}mM/min\;and\;1.14{\times}10^{-4}mM/min$, respectively.

• Applied Biological Chemistry
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• 제48권4호
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• pp.364-369
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• 2005

Trichoderma harzianum이 생산하는 ${\beta}-mannanase$의 최적 사면배지조성은 2.0% malt extract, 2.0% glucose, 0.1% peptone, 2.0% agar로, 효소생산 배지조성은 3.0% cellulose, 3.0% C.S.L, 1% $KH_2PO_4$, 0.2% $(NH_4){_2}SO_4$로 결정되어 $28^{\circ}C$, 130 rpm, 진탕배양법으로 조효소액을 생산하였다. ${\beta}-mannanase$의 최적 pH와 최적온도는 pH 4.5, $60^{\circ}C$에서 최대 상대활성을 나타내었다. Locust bean gum에 대한 효소적 가수분해 pattern을 TLC에 의해 검토한 결과 반응초기부터 반응말기까지 주요 생성물은 단당류, 중합도 4와 7의 올리고당으로 확인되었다. 중합도별 올리고당을 조제하기 위하여 activated carbon column chromatography에 250 ml/hr유속으로 tube당 20 ml씩 ethanol $0{\sim}50%$의 gradient법으로 분리 회수하였고, 분리된 각각의 올리고당 중 중합도4는 TLC $R_f$ value상으로 ${\beta}-1,4-mannotetraose$로, 중합도 7은 Methylation method에 의해 M-M-M-M-M 구조로 //G-G 동정되었다. (G-와 M-은 각각 ${\alpha}-1,6-D-galactosidic,\;{\beta}-1,4-mannosidic$ 결합을 나타냄). Bifidobacterium longum에 대한 생육활성을 기존의 MRS media와 탄소원으로 중합도 4와 7의 올리고당으로 대체하였을때 3.4배, 4.3배의 생육활성이 증가하였고, Bifidobacterium bifidum에 대해서는 1.2배, 1.1배의 낮은 활성을 나타내었다.

• Bulletin of the Korean Chemical Society
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• 제13권2호
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• pp.158-162
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• 1992

Hydrocarbon solution of $(PPh_3)_2(CO)MOSO_2CF_3(M=Rh$, Ir)reacts rapidly with Pyrazine or 4,4'-bipyridyl to yield dinuclear metal complexes $[(PPh_3)_3(CO)M({\mu}-pyrazine)M(CO)(PPh_3)_2](SO_3CF_3)_2$ (I: M= RhH; III: M=Ir) or [$(PPh_3)_2$(CO)M(${\mu}$-44'-bipyridyl)M(CO)$(PPh_3)_2](SO_3CF_3)_2$, (II: M=Rh; IV: M=Ir), respectively. Compounds, I, II, III, and IV were characterized by $^1H-NMR$, $^{13}C-NMR$, $^{31}P-NMR$, and infrared spectrum. Ethanol solution of $(PPh_3)_2(CO)MOSO_2CF_3$ (M=Rh, Ir) also reacts with $(TBA)_2$M'$(CN)_4$ (M'=Pd, Pt) to yield trinuclear metal complexes [$(PPh_3)_2$(CO)dM-NCM'$(CN)_2$CN-M(CO)$(PPh_3)_2]$ (V : M=Rh, M'=Pd; VI : M=Rh, M'=Pt; VII: M=Ir, M'=Pd; VIII: M=Ir, M'=Pt). The trinuclear metal complexes V, VI, VII, and VIII are bridged by the cyanide groups. The infrared spectrum of V, VI, VII, and VIII supports the presence of the bridged cyanide and terminal cyanide group.

• Ecology and Resilient Infrastructure
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• 제7권4호
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• pp.308-319
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• 2020

본 논문은 경북 영천시 서문보에 최근 건설된 다기능 어도의 현장 적용성을 검토하기 위해 해석 변수를 R/C Slab (S1), R/C+S/C Slab (S2) 및 지하이동통로 규격 (가로 × 세로)을 1.4 × 0.2 m, 1.4 × 0.3 m, 1.4 × 0.6 m와 유속 0.8 m/s, 1.2 m/s, 1.6 m/s 으로 구분하여 해석한 결과 서문보 설계식 안전성을 평가하였다. 서문보의 설계식 보다 R/C+S/C Slab타입이 지하이동통로 출구부는 휨모멘트와 최대응력은 각각 16 - 33%, 24 - 32%, 측벽은 각각 17 - 33%, 20 - 36%, 상부슬래브인 경우도 19 - 33%, 9 - 28% 적게 나타났다. 따라서 최대응력과 휨모멘트가 R/C+S/C Slab 타입이 구조 안전성이 확보되는 것으로 나타났다. 따라서 지하통로는 휨모멘트와 최대 응력이 각각 14%, 18%, 측벽은 17%, 15% 상부슬래브는 16%, 11%의 보완이 요구되는 것으로 판단된다. 이러한 결과는 지하이동통로 규격이 서문보 규격과 동일한 1.4 × 0.3 m 일 때가 1.4 × 0.2 m, 1.4 × 0.6 m보다 안전성이 가장 유리한 것으로 확인되었다. 또한 해석 및 분석 결과를 근거로 서문보 규모의 다기능 어도 적용 시 기본 자료로 활용이 기대된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 하계학술대회 논문집
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• pp.444-447
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• 2000

The spinel L $i_{1-x}$ M $n_2$ $O_4$has been synthesized by the solid-state reaction. L $i_{l-x}$M $n_2$ $O_4$which includes a mixture of LiOH . $H_2O$ and Mn $O_2$prepared by preliminary heating at 35$0^{\circ}C$ for 12hr. L $i_{l-x}$M $n_2$ $O_4$fired at temperature range from 75$0^{\circ}C$ for 48hr. The structure and the electrochemical characteristics of spinel to L $i_{1-x}$ M $n_2$ $O_4$which is fabricated by changing sintering condition from starting materials are investigated. The cyclic voltammetric measurement was performed using 3 electrode cells. Electrode specific capacity and cycle life behavior were tested in a 3.0~4.2V range at a constant current density of 0.45mA/c $m^2$. To improve the cycle performance of spinel L $i_{l-x}$M $n_2$ $O_4$as the cathode of 4V class lithium secondary batteries, spinel phases L $i_{1-x}$ M $n_2$ $O_4$were Prepared at various lithium. The results showed that discharge capacity of L $i_{l-x}$M $n_2$ $O_4$varied at lithium quantity decrease with increasing lithium add quantity. The discharge capacities of L $i_{0.925}$M $n_2$ $O_4$and LiM $n_2$ $O_4$revealed 108 and 117mAh/g, respectively.spectively.y.