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Statistical Optimization of Biosurfactant Production from Aspergillus niger SA1 Fermentation Process and Mathematical Modeling

  • Mansour A. Al-hazmi;Tarek A. A. Moussa;Nuha M. Alhazmi
    • Journal of Microbiology and Biotechnology
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    • v.33 no.9
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    • pp.1238-1249
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    • 2023
  • In this study, we sought to investigate the production and optimization of biosurfactants by soil fungi isolated from petroleum oil-contaminated soil in Saudi Arabia. Forty-four fungal isolates were isolated from ten petroleum oil-contaminated soil samples. All isolates were identified using the internal transcribed spacer (ITS) region, and biosurfactant screening showed that thirty-nine of the isolates were positive. Aspergillus niger SA1 was the highest biosurfactant producer, demonstrating surface tension, drop collapsing, oil displacement, and an emulsification index (E24) of 35.8 mN/m, 0.55 cm, 6.7 cm, and 70%, respectively. This isolate was therefore selected for biosurfactant optimization using the Fit Group model. The biosurfactant yield was increased 1.22 times higher than in the nonoptimized medium (8.02 g/l) under conditions of pH 6, temperature 35℃, waste frying oil (5.5 g), agitation rate of 200 rpm, and an incubation period of 7 days. Model significance and fitness analysis had an RMSE score of 0.852 and a p-value of 0.0016. The biosurfactant activities were surface tension (35.8 mN/m), drop collapsing (0.7 cm), oil displacement (4.5 cm), and E24 (65.0%). The time course of biosurfactant production was a growth-associated phase. The main outputs of the mathematical model for biomass yield were Yx/s (1.18), and µmax (0.0306) for biosurfactant yield was Yp/s (1.87) and Yp/x (2.51); for waste frying oil consumption the So was 55 g/l, and Ke was 2.56. To verify the model's accuracy, percentage errors between biomass and biosurfactant yields were determined by experimental work and calculated using model equations. The average error of biomass yield was 2.68%, and the average error percentage of biosurfactant yield was 3.39%.

Development of Legibility Distance Models for Each Color according to Luminance under Foggy Condition (안개시 휘도에 따른 색상별 판독거리 산출 모형 개발)

  • Kim, Chul-Yong;Lee, Sang-Soo;Youn, IL-Soo;Oh, Ju-sam
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.19 no.3
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    • pp.52-61
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    • 2020
  • This study developed a legibility distance(LD) model for three colors used in VMS according to luminance under foggy conditions through experiments. For this, a simulated environment was created, and LD values of red, green, and yellow color were collected by increasing the luminance. From the test results, the LDs for red at luminance levels of 2,000 cd/㎡ and 20,000 cd/㎡ and a visibility distance of 10m were 10.2m and 18.8m, respectively, which was an improvement of 84%. The LD was improved by 34% and 17% at a visibility distance of 20 m and 30 m, respectively. For green, the LD was improved by 69%, 63%, and 19% at visibility distance of 10m, 20m, 30m, respectively. For yellow, the LD was improved by 100%, 47%, and 36% at visibility distance of 10m, 20m, 30m, respectively. The LD models for three colors were developed by using two independent variables, luminance value and visibility distance. The adjusted coefficients of the red, green, and yellow model equations were calculated as 0.982, 0.978, and 0.915, respectively, and the explanatory power of all models was very high.

Reproduction of Shallow Tides and Tidal Asymmetry by Using Finely Resolved Grid on the West Coast of Korea (서해연안 상세해상을 통한 천해조석 및 조석비대칭 재현)

  • Suh, Seung-Won
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.23 no.4
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    • pp.313-325
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    • 2011
  • Finite element grid system using h-refinement on the Yellow Sea was constructed based on previous study (Suh, 1999b) from 14 K to 210 K and special attention was concentrated on refining the coastal zone. In grid generation, depth change between adjacent points and non-dimensional tidal wave length ratio were considered. As a result approximately a quarter of the total nodes are located nearby 5 m of shallow area. Accurate bathymetry data using 30's and ETOPO1 with open boundary conditions of 8 major tidal constituents extracted automatically from FES2004 have been applied. In tidal simulation a 3-dimensional nonlinear harmonic model was setup and tidal amplification due to changes in vertical turbulent and bottom friction were simulated. In this study not only 8 major tidal constituents but also nonlinear shallow tides $M_4,$, $MS_4$ and long period $M_f,$, $M_{sf}$ were reproduced. It is found that implication of spatial variation of friction coefficient plays a very important role in reproduction of astronomical and shallow tides which are computed by iterative computation of nonlinear terms. Also it should be considered differently with respect to tidal periods. To understand the distribution of tidal asymmetry, amplitude ratio of $M_4/M_2$ and phase differences $2g(M_2)-g(M_4)$ were calculated. Tidal distortion ratio marks up to 0.2 on the west coast showing shallow coastal characteristics and somewhat wide range of ebb-dominances in front of Mokpo area are reproduced.

A Study on the Architectural Planning of traditional herbal medicine distribution supporting facilities (한약재유통지원시설의 건축계획에 관한 연구)

  • Bae, Joa-Sup;Oh, Jong-Hee;Kang, Won-Pil
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.14 no.2
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    • pp.53-64
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    • 2008
  • This study is the architectural planning of 5 distribution supporting facilities for traditional herbal medicine cultivated in the rural areas. The main function of the facility consists of storage, pre-treatment/processing, loading/unloading and the assistant function consists of inspection, office, exhibition/sale, technical equipment etc. The planning shows the modular plan and the section plan of the storage space reflecting the shape and size of storage container, the action radius of carrier and the possibilities of space lease. The total floor area of the storage is 3,192$m^2$ consisting of 27 space. The total floor area of the pre-treatment/processing is 1,488$m^2$ consisting of 7 space. The total floor area of the loading/unloading is 329$m^2$ consisting of 4 space. The total floor area of each facility storage is 8,284$m^2$ including of public space 2,170$m^2$. Also this planning shows the preliminary design, "fundamental model design" for 5 rural government BTL project. Therefore, this result of planning will be the guidelines of the RFP(Request For Proposal) for the private sector company interested in this BTL project.

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Development of Three-dimensional Baroclinic Hydrodynamic Model and flow Patterns of the Suyoung Bay (3차원 경합 海水流動 모델의 開發과 水營蠻의 폐수유동)

  • 김차겸;이종섭
    • 한국해양학회지
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    • v.28 no.2
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    • pp.86-100
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    • 1993
  • Three-dimensional baroclinic hydrodynamic model, BACHOM-3, is developed using ADI finite difference scheme. The model is applied to a uni-nodal standing wave in a rectagular basin. The model results for the surface elevation and velocities coincide with the analytical results. To verify the field applicability of the model and to investigate the flow patterns of the Suyoung Bay in Pusan, Korea, the model is applied to the bay. The numerically predicted velocity predicted velocity fields during spring tide at normal river flow are compared with field measurements, the comparisons show good agreement. A clockwise residual circulations at the first level (depth = 0∼2m) and the second level (depth=2∼5 m) of the central part of the bay occur, and the ebb flow is stronger than the flood flow. Computed velocity fields show that the phase difference of velocities between the surface layer and bottom layer occurs and the phase lag increases with height from the bottom. Then, the model is applied successfully for the computation of flow fields considering flood river flow and wind effects. When the wind is blowing toward the land from the sea, the flow patterns at the surface layer correspond with the wind direction, but the flow patterns at the near solid boundary of the lower layer show opposite currents to the wind direction.

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A Study on the Method for Converting the Unit Database from Training-model into Analysis-model : Focused on the 'Chang-Jo21' and 'Vision21' model (훈련용 워게임 모델의 부대 DB를 분석용 워게임 모델에 재사용하기 위한 변환방법 연구 : 창조21모델과 비전21모델을 중심으로)

  • Lee, Yong-Bok;Park, Min-Hyoung;Kim, Yeek-Hyun
    • Journal of the Korea Society for Simulation
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    • v.28 no.2
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    • pp.159-167
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    • 2019
  • In the field of defense M&S, we are actively pursuing researches that interoperable multiple war game models to simulate various combat environments at the same time. Although the 'unit DB(Database)' for operating the war game models is originated from the identical data, it has been recognized that the method of expressing the attribute of the data is different and the cross reference is impossible. As a result, it makes unnecessary time and effort in establishing the same unit DB in the organizations that operate the war game model. In this study, a method of reusing the unit DB of the training war game model to the analysis war game model with similar resolution and simulated logic was applied to the actual field. For this purpose, we defined the procedure for converting the unit DB by analyzing metadata of the 'Chang-Jo21', a combat training model for corps and division, and the 'Vision21', an analysis model for corps and division operation plan. And we introduced an algorithm that can map different metadata of two unit DBs. This study was meaningful as the first attempt to map and integrate heterogeneous metadata semantically for the reuse of unit DB between different war game models in defense M&S field. Also, it provided implications for the necessity of paradigm shift that reuse of the unit DB between two different war game models is possible and the need for standardization of the unit DB metadata in the defense M&S filed.

Enzymatic Synthesis of Cephaloglycin

  • Doo-Hyun Nam;Heon-Soo Sohn;Dewey D. Y. Ryu
    • Bulletin of the Korean Chemical Society
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    • v.4 no.2
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    • pp.72-76
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    • 1983
  • Cephaloglycin was synthesized directly from D-${\alpha}$ -phenylglycine methyl ester and 7-aminocephalosporanic acid using whole cell enzyme of Xanthomonas citri (IFO 3835). Some optimal conditions for cephaloglycin synthesis were investigated, and yield improvements for its production by several methods were attempted. Using the whole cell enzyme system, the reaction kinetic model for cephaloglycin synthesis is proposed, and the kinetic constants for D-${\alpha}$ -phenylglycine methyl ester hydrolysis, cephaloglycin synthesis, and cephaloglycin hydrolysis were determined. The $K_m$ values of D-${\alpha}$-phenylglycine methyl ester, 7-aminocephalosporanic acid, and cephaloglycin were 11 mM, 24 mM, and 167 mM, and $K_i$ value of D-${\alpha}$-phenylglycine was 15 mM, respectively. The pattern of product inhibition was found to be competitive one.

Model System Study for the Mutagenicity of Sugar-Glycine Systems

  • Lee, Jae-Hwan;Shin, Han-Seung
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.839-841
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    • 2008
  • The mutagenicity after heating of different sugars (glucose, fructose, galactose, and tagatose) on the non-enzymatic browning reaction in different sugars and glycine model system was investigated. The model system containing 0.2 M glycine and 0.2 M of different sugars in 10 mL water was heated at $150{\pm}5^{\circ}C$ for 30 min. After heating, degree of non-browning reaction intensity and mutagenicity using Salmonella typhimurium TA 98 were examined. Heated glycine model systems containing different sugars increased their mutgenicity ranged from 30 to 372 revertant colonies. After heating for 40 min, mutagenicity was achieved with glycine model systems containing 4 different sugars with by 145, 356, 206, and 369 revertants per plate, respectively. The glycine model systems containing fructose or tagatose were significantly (p<0.05) higher mutagenic activity than glycine model systems containing glucose or galactose after 40 min of heating. The linear regression between Maillard reaction intensity and mutagenic activities (slope=32.38, $R^2=0.93$) indicates that mutagenicity could be fully ascribed to Maillard reaction products.

Analysis and Prediction of Anchovy Fisheries in Korea ARIMA Model and Spectrum Analysis (한국 멸치어업의 어획량 분석과 예측 ARIMA 모델 및 스펙트럼 해석)

  • PARK Hae-Hoon;YOON Gab-Dong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.2
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    • pp.143-149
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    • 1996
  • Forecasts of the monthly catches of anchovy in Korea were carried out by the seasonal Autoregressive Integrated Moving Average (ARIMA) model and spectral analysis. The seasonal ARIMA model is as follows: $$(1-0.431B)(1-B^{12})Z_t=(1-0.882B^{12})e_t$$ where: $Z_t=value$ at month $t;\;B^{p}$ is a backward shift operator, that is, $B^pZ_t=Z_{t-p};$ and $e_t=error$ term at month t, which is to forecast 24 months ahead the anchovy catches in Korea. The prediction error by the Box-Cox transformation on monthly anchovy catches in Korea was less than that by the logarithmic transformation. The equation of the Box-Cox transformation was $Y'=(Y^{0.58}-1)/0.58$. Forecasts of the monthly anchovy catches for $1991\~1992$, which were compared with the actual catches, had an absolute percentage error (APE) range of $1.0\~63.2\%$. Total observed annual catches in 1991 and 1992 were 170,293 M/T and 168,234 M/T respectively, while the predicted catches were 148,201 M/T and 148,834 M/T $(API\;13.0\%\;and\;11.5\%,\;respectively)$. The spectrum analysis of the monthly catches of anchovy showed some dominant fluctuations in the periods of 2.2, 6.1, 10.2 12.0 and 14.7 months. The spectrum analysis was also useful for selecting the ARIMA model.

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Sorption of Tc(IV) in Saline Solutions - I. Sorption on MX-80 and Granite in Ca-Na-Cl Solutions

  • Shinya Nagasaki;Zhiwei Zheng;Jianan Liu;Jieci Yang;Tammy (Tianxiao) Yang
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.22 no.3
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    • pp.273-285
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    • 2024
  • Technetium-99 is identified as an element of interest for the safety assessment of a deep geological repository for used nuclear fuel. The sorption behavior of Tc(IV) onto MX-80 and granite in Ca-Na-Cl solutions of varying ionic strength (0.05-1 mol·kgw-1 (m)) and across a pHm range of 4-9 was studied in this paper. Sorption of Tc(IV) was found to be independent of ionic strength in the range of 0.05 to 1 m for both MX-80 and granite. Sorption of Tc(IV) on MX-80 increased with pHm from 4 to 7 and then decreased with pHm from 8 to 9. Sorption of Tc(IV) on granite gradually increased with pHm from 4 to 8 and then became almost constant or slightly decreased with pHm from 8 to 9. A 2 site protolysis non-electrostatic surface complexation and cation exchange sorption model successfully simulated sorption of Tc(IV) on MX-80 and granite. Optimized values of surface complexation constants (log K0) are proposed.