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Effect of Cytosine Analogues on Cytosine Deaminase from Aspergillus fumigatus IFO 5840 (Aspergillus fumigatus IFO 5840의 Cytosine Deaminase에 미치는 Cytosine Analogue의 영향)

  • 김재근
    • The Korean Journal of Food And Nutrition
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    • v.10 no.1
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    • pp.53-59
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    • 1997
  • In this study investigated the effect of cytosine deaminase activity from Aspergillus fumigatus IFO 5840 by cytosine analogues. The results were as follows. The enzyme was strongly inibited by 2-thiouracil, 2-thiocytosine, 6-azacytosine and 2-mercaptopyrimidine. The half inhibitory concentration(HIC) of 2-thiocytosine and 6-azacytosine on cytosine deaminase was 0.80mM and 1.15mM, respectively. The enzyme was inhibited at a certain level by addition of 2-thiocytosine immediately, but was maintained to some extend under the inhibited state by 6-azacytosine in proportion to reaction time. Regardless of kinds of substrate such as cytosine and 5-fluorocytosine, 2-thiocytosine and 6-azacytosine showed action as inhibitors, 2-thiocytosine inhibited cytosine deaminase activity about twice as strong as 6-azacytosine. The enzyme, when cytosine was used as a substrate, was revealed the pattern of competitive inhibition by 2-thiocytosine and 6-azacytosine, The ki value for these compounds was 4.5$\times$10-4M and 1.756$\times$10-3M, respectively. At this point, the Hill coefficient for cytosine, 2-thiocytosine and 6-azacytosine was 1.80, 1.81 and 2.45, respectively.

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Four-Valued Cross-Correlation Function between Two Maximal Linear Recursive Sequences (최대 주기의 두 선형 순환 수열 사이의 4개의 값을 갖는 상호상관함수)

  • Choi, Un-Sook;Kim, Sung-Jin;Kim, Han-Doo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.7 no.6
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    • pp.1369-1375
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    • 2012
  • One of important problems in the theory of sequences is to determine the values and number of occurrences of each value taken on by the cross-correlation. In this paper, we find the values and the number of occurrences of each value of cross-correlation between an m-sequence u(t) of period $2^n-1$ and its decimation $u(dt)(0{\leq}t{\leq}2^n-2)$ where n=2m, 2s|m and $d=(2^{2m}+2^{2s+1}-2^{m+s+1}-1)/(2^s-1)$. Also we show that a family of decimations leads to a four-valued cross-correlation.

Kinetic Study using Ultrasonic Technique on the Dissociation-Recombination Reaction between Micelle and Counter-ion in Dodecyl Pyridinium Chloride Solution (超音波를 利用한 Dodecyl Pyridinium Chloride 水溶液中의 미셀과 반대이온 사이의 解離-再結合反應의 反應速度論的 硏究)

  • Kun Moo Lee
    • Journal of the Korean Chemical Society
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    • v.20 no.3
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    • pp.193-197
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    • 1976
  • The ultrasonic absorption has been measured in aqueous solution of dodecyl pyridinium chloride (DPC) at $20^{\circ}C$ over the frequency range of 0.1${\sim}$90 Mc.The excess absorption was observed only in solutions in which the concentration was higher than the critical micellar concentration(cmc). The mechanism for this feature was attributed to the reaction $M_2\;{\rightleftharpoons}\;M_1\;+\;1.2Cl^-$ Where $M_2$ and $M_1$ and M1 are two types of micelle. The rate constants of the forward and the reverse reactions were $6.6{\times}10^5 sec^{-1}\;and\;2.7{\times}10^11sec^{-1}mol^{-1.2}$respectively. Some kinetic charateristics including the free energy, enthalpy and entropy were calculated.

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$H_{2}$ production of photosynthetic bacteria transferred TOL plasmid from flavobacterium odoratum (Flavobacterium odoratum의 TOL 플라스미드를 전달받은 광합성세균으로부터의 수소 생성)

  • 오순옥;조인성;이희경;민경희
    • Korean Journal of Microbiology
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    • v.29 no.6
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    • pp.408-415
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    • 1991
  • TOL plsmid size of Flavobacterium odoratum SUB53 was estimated as 83 Md and the optimum concentration of m-toluate degradation by TOL plasmid was 5 mM. $H_{2}$ production by Rhodopseudomonas sphaeroides KCTC1425 was largely dependent on nitrogenase activity and showed the highest at 30 mM malate with 7 mM glutamate as nitrogen source. Nitrogenase activities were inhibited by 0.3 mM $NH_{4}^{+}$ions, to be appeared the decrease of $H_{2}$ production. Conjugation of TOL plasmids from F. odoratum SUB53 and Pseudomonas putida mt-2 to R. sphaeroides showed the optimum at the exponential stage of recipient cells in presence of helper plasmid pRK2013. According to the investigation of catechol-1,2-oxygenase (C-1, 2-O) and catechol-2,3-oxygenase (C-2,3-O) activities of R. sphaeroides C1 (TOL SUB53) and C2 (TOL mt-2), the gene for C-2,3-O is located on TOL plasmid and gene for C-1, 2-O on the chromosome of R. sphaeroides. m-Toluate was biodegraded by TOL plasmid in R. sphaeroides C1 and C2, presumably to be produced $H_{2}$ gas from the secondary metabolites of m-toluate.e.

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Physiological Response of Four Corn Cultivars to Soil Salinity (토양염농도에 따른 식용옥수수 품종들의 생장특성)

  • Kim, Sun;Choi, Weon-Young;Jeong, Jae-Hyeok;Lee, Kyung-Bo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.293-298
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    • 2014
  • This study conducted experiments on the reclaimed land of Saemangeum located in Jeongrabuk-do in order to gain basic information about growth characteristics and yield ability according to soil salinity. Having soil excluding salt as a control group, this study adjusted the specimens' soil salinity to level 4 and then planted four varieties including Ilmichal Corn to investigate the growth or grain yield according to salinity. About the corn establishment rate according to soil salinity, over 97% up to $3.2dS\;m^{-1}$ was established, and then, it was reduced gradually according to the increase of concentration. According to the salt concentration of soil more required growth duration from seeding to heading comparing to non-treatment salt was delayed, at $1.6dS\;m^{-1}$, two days were delayed, at $3.2dS\;m^{-1}$, four to six days were delayed differently by varieties, and at $4.8dS\;m^{-1}$, six to 10 days were delayed. About the plant fresh weight according to soil salinity, Chalok 4 and Eolrukchal indicated 93%~97% or so compared with the salt-free one at $1.6dS\;m^{-1}$, and Chalok No. 4 showed 79% at the salinity of $3.2dS\;m^{-1}$, too, and it was a relatively higher growth percentage than those of the other varieties. In terms of dried seed weight according to soil salinity, compared with the corns cultivated in the control group, averagely 12.1% was lowered at the time of cultivation at $1.6dS\;m^{-1}$, and $3.2dS\;m^{-1}$ 40% was lowered, and about 70% was lowered at $4.8dS\;m^{-1}$. According to the result of examining the point of time that dried seed start to reduce due to soil salinity with the regression equation, soil salinity which starts the reduction of grain weight is $1.67dS\;m^{-1}{\sim}2.18dS\;m^{-1}$, and it differs by varieties, and EC of 50% that the yield reduces in half is $2.96dS\;m^{-1}{\sim}4.45dS\;m^{-1}$. And the degree of influence on each of the growth factors according to soil salinity is founded to be in the order of establishment rate

Analysis of Heating and Cooling Energy Consumption in Rental and Sales Apartments in Busan (부산시 임대아파트 및 분양아파트의 냉난방에너지 소비량 분석)

  • Lee, Kyung-Hee;Lee, Jun-Gi
    • Land and Housing Review
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    • v.12 no.3
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    • pp.79-85
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    • 2021
  • This study analyzed the energy consumption differences between rental and owner owned (purchased) apartments in Busan, South Korea during the cooling and heating seasons. Analysis revealed that the average electricity consumed for cooling was 2.5 kWh/m2·yr for rental apartments and 2.3 kWh/m2·yr for purchased apartments, a difference of 0.2 kWh/m2·yr. The average electrical heating energy consumption was 3.3 kWh/m2·yr for rental apartments and 2.2 kWh/m2·yr for purchased apartments, a difference of 1.1 kWh/m2·yr. It was estimated that the use of electric blankets and heaters was higher in rental apartments than purchased apartments resulting in higher electrical heating energy consumption. The average gas heating energy consumption was 7.0 kWh/m2·month for rental apartments and 6.8 kWh/m2·month for purchased apartments. When electricity and gas usage was combined for heating, the average total heating energy consumption was 10.3 kWh/m2·month for rental apartments and 9.0 kWh/m2·month for purchased apartments. This indicates that rental apartments consume 1.3 kWh/m2·month more energy than purchased apartments during heating season. Overall, rental apartments consume more energy than purchased apartments during both the cooling and heating seasons.

Overproduction of Lignin Peroxidase from Phanerochaete chrysosporium PSBL-1 (Phanerochaete chrysosporium PSBL-1의 배양조건 최적화를 통한 Lignin Peroxidase의 과량생산)

  • 정병철;한윤전;장승욱;정욱진;원유정
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.228-235
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    • 2000
  • Until now, it was diIliculi to overproduce lignin peroxidase(LiP) fiom Pl~anemchaete ch~ysosporium since the lack of optimized growth conditions. In this paper, we optimized the LIP production conditions and monitored LIP isozyines of fl chqsospoi.ium PSBL-1. The optimized condition includes sponge matrix support, no addition of $MnSO_4$, excess addition of niixogen source(48 inM diarmnonium), and addition of stabilizer(2 mM verakyl alcohol). Finally we obtained Lip activity of 1,800 unitsll. HI isozyne was overproduced when inyceliuin was cultivated in media containing $Mn^{2+}$ (2.73 inM) and excess nitrogen(48 11d4 diannnonium). Three azo dyes(acid yellow 9, congo ued, orange IT; each concenimtion of50 $\mu$M) we1-e rapidly decolorized within 2 inins by 0.4 un~t or Lip.

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진행암환자에서 rh GM-CSF(recombinamt human granulocyte-macrophage colony stimulating factor)의 제 1 상 및 약동학 연구 평가를 위한 Protocol 개발 연구

  • 노재경;최진혁;노형근;김범수;한지숙;김병수
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.95-95
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    • 1993
  • 대상환자는 15예로, 14예에서 평가가능하였다. 남녀비는 8:6, 중앙연령 32세(10-70세)이었고, 대상질환은 악성골육종 7예, 악성임파종 2예, 위암2예, 폐암 2예, 그리고 자궁악성육종 1예였다. CSF 50 ug/$m^2$ 3예, 100 ug/$m^2$ 3예,150 ug/$m^2$ 3예, 250 ug/$m^2$ 3예,350 ug/$m^2$ 3예, 500 ug/$m^2$ 6예, 700 ug/$m^2$ 용량 3예에서 시행되었다. 1주기 시행한 환자는 7예, 2주기 5예, 3주기, 4주기 각각 1예씩이었다. 부작용은 50-150ug/$m^2$에서 WHO grade I의 발열, 전신쇠약, 식욕부진 등이 관찰되었으나 grade II이상의 부작용은 없었다. 250 ug/$m^2$ 이상의 용량에서도 grade II의 발열이 관찰되었을 뿐 다른 중증의 부작용은 관찰되지 않았다. 최고용량인 700 ug/$m^2$ 에서도 grade II의 발열외의 중한 부작용은 관찰되지 않았다. 각 용량에 따른 백혈구 증가율(%투여제2일/투여제1일)은 130-500% 이었다. 시행된 약동태는 CSF 투여 2-4 시간 후 최고치 (0.42-15.4 ng/ml)를 나타내고 투여 12시간까지 0.2-2 ng/ml의 농도로 지속되었다. 소변내 CSF 배설량은 총투여량의 1% 미만이었다. CSF중화항체는 전예에서 검출되지 않았다.

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항암화학요법제에 의하여 골수억제가 수반된 진행암환자에서 rh GM-CSF(recombinamt human granulocyte-macrophage colony stimulating factor)의 제 1 상 및 약동학 연구

  • 노재경;최진혁;노형근;김범수;한지숙;김병수
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.68-68
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    • 1993
  • 대상환자는 15예로, 14예에서 평가 가능하였다. 남녀비는 8:6, 중앙연령 32세(10-70세)이었고, 대상질환은 악성골육종 7예, 악성임파종 2예, 위암2예, 폐암 2예, 그리고 자궁악성육종 1예였다. CSF 50 ug/$m^2$ 3예, 100 ug/$m^2$ 3예,150 ug/$m^2$ 3예, 250 ug/$m^2$ 3예, 350 ug/$m^2$ 3예, 500 ug/$m^2$ 6예, 700 ug/$m^2$ 용량 3예에서 시행되었다. 1주기 시행한 환자는 7예, 2주기 5예, 3주기, 4주기 각각 1예씩이었다. 부작용은 50-150ug/$m^2$에서 WHO grade I의 발열, 전신쇠약, 식욕부진등이 관찰되었으나 grade II이상의 부작용은 없었다. 250 ug/$m^2$ 이상의 용량에서도 grade II의 발열이 관찰되었을 뿐 다른 중증의 부작용은 관찰되지 않았다. 최고용량인 700 ug/$m^2$ 에서도 grade II의 발열 외의 중한 부작용은 관찰되지 않았다. 각 용량에 따른 백혈구 증가율(%투여제2일/투여제1일)은 130-500% 이었다. 시행된 약동태는 CSF 투여 2-4 시간후 최고치 (0,42-15.4 ng/ml)를 나타내고 투여 12시간까지 0.2-2 ng/ml의 농도로 지속되었다. 소변내 CSF 배설량은 총투여량의 1% 미만이었다. CSF 중화항체는 전예에서 검출되지 않았다.

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Electrochemical Behavior of Bis(2,2'-Bipyridyl) Copper(II)-Sodium Dodecyl Sulfate Solution in the Presence of $MgCl_2$ (염화마그네슘이 있을 때 비스(2,2'-비피리딜)구리(II)-도데실황산나트륨 용액의 전기화학적 거동)

  • Ko, Young Chun;Chung, Keun Ho
    • Analytical Science and Technology
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    • v.10 no.2
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    • pp.126-130
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    • 1997
  • Electrochemical behavior of 1.0mM bis(2,2'-bipyridyl)copper( II )(${Cu(bpy)_2}^{2+}$) in 100mM NaCl+27mM $MgCl_2$ solution with and without sodium dodecyl sulfate(SDS) is investigated. In the presence of SDS, $E_{pa}$ and $E_{1/2}$ of ${Cu(bpy)_2}^{2+/1+}$ shifts to the positive direction compared to the SDS free. Teh intersection of two lines on ${\Delta}E_p$ vs. -log[SDS] plot for 1.0mM $Cu(bpy)_2(NO_3)_2$ in 100mM NaCl+27mM $MgCl_2$ solution is a critical micelle concentration(CMC). Reduced form ${Cu(bpy)_2}^{1+}$ in the presence of $MgCl_2$ is more easily associated to dodecyl sulfate anion($DS^-$) than oxidized ${Cu(bpy)_2}^{2+}$. As the ionic strength is increased by the addition of multiply charged cations($MgCl_2$), the double layer becomes more compact and micelle formation is more difficult.

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