• Molecules and Cells
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• v.43 no.5
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• pp.479-490
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• 2020

Interleukin-9 (IL-9) is well known for its role in allergic inflammation. For cancer, both pro- and anti-tumor effects of IL-9 were controversially reported, but the impact of IL-9 on tumor metastasis has not yet been clarified. In this study, IL-9 was expressed as a secretory form (sIL-9) and a membrane-bound form (mbIL-9) on B16F10 melanoma cells. The mbIL-9 was engineered as a chimeric protein with the transmembrane and cytoplasmic region of TNF-α. The effect of either mbIL-9 or sIL-9 expressing cells were analyzed on the metastasis capability of the cancer cells. After three weeks of tumor implantation into C57BL/6 mice through the tail vein, the number of tumor modules in lungs injected with IL-9 expressing B16F10 was 5-fold less than that of control groups. The percentages of CD4⁺ T cells, CD8⁺ T cells, NK cells, and M1 macrophages considerably increased in the lungs of the mice injected with IL-9 expressing cells. Among them, the M1 macrophage subset was the most significantly enhanced. Furthermore, peritoneal macrophages, which were stimulated with either sIL-9 or mbIL-9 expressing transfectant, exerted higher anti-tumor cytotoxicity compared with that of the mock control. The IL-9-stimulated peritoneal macrophages were highly polarized to M1 phenotype. Stimulation of RAW264.7 macrophages with sIL-9 or mbIL-9 expressing cells also significantly increased the cytotoxicity of those macrophages against wild-type B16F10 cells. These results clearly demonstrate that IL-9 can induce an anti-metastasis effect by enhancing the polarization and proliferation of M1 macrophages.

• Korean journal of applied entomology
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• v.43 no.1
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• pp.15-20
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• 2004

Leaf damage by apple leafminer, Lyonetia prunifoliella, was surveyed on Fuji apple orchards with seedling rootstock from 1992 to 2001 and with M.9 rootstock from 1999 to 2001. The damage was negligible in apple orchards with seedling rootstock as 0.15% in 1992 and 0.06% in 1993. However it sharply increased to more than 3.39% since 1994, reaching at peak damage of 6.28% to 7.78% in 1996 to 1998. Damages on apple orchards with M.9 rootstock from 1999 to 2001 were higher as 6.09% to 15.3%, compared with those with seedling rootstock in the same years. In seedling rootstock apple, the damage was highest on september but in M.9 rootstock apple, the difference was not significant. Leaf damage showed no differences between cultivars including 4 Korean cultivars and Fuji. These results may explain that increase of M.9 rootstock apple orchards is responsible for the recent outbreak of the leafminer in Korea.

• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.2
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• pp.77-82
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• 2010

Purpose: As PET/CT come into wide use, it caused increasing of expose in clinical use. Therefore, Korea Food and Drug Administration issued Patient DRL (Diagnostic Reference Level) in CT scan. In this study, to build the basis of patient dose reduction, we analyzed effective dose in transmission scan with CT scan. Materials and Methods: From February, 2010 to March 180 patients (age: $55{\pm}16$, weight: $61.0{\pm}10.4$ kg) who examined $^{18}F$-FDG PET/CT in Asan Medical Center. Biograph Truepoint 40 (SIEMENS, GERMANY), Biograph Sensation 16 (SIEMENS, GERMANY) and Discovery STe8 (GE healthcare, USA) were used in this study. Per each male and female average of 30 patients doses were analyzed by one. Automatic exposure control system for controlling the dose can affect the largest by a patient's body weight less than 50 kg, 50-60 kg less, 60 kg more than the average of the three groups were divided doses. We compared that measured value of CT-expo v1.7 and ImPACT v1.0. The relationship between body weight and the effective dose were analyzed. Results: When using CT-Expo V1.7, effective dose with BIO40, BIO16 and DSTe8 respectably were $6.46{\pm}1.18$ mSv, $9.36{\pm}1.96 $mSv and $9.36{\pm}1.96$ mSv for 30 male patients respectably $6.29{\pm}0.97$ mSv, $10.02{\pm}2.42$ mSv and $9.05{\pm}2.27$ mSv for 30 female patients respectably. When using ImPACT v1.0, effective dose with BIO40, BIO16 and DSTe8 respectably were $6.54{\pm}1.21$ mSv, $8.36{\pm}1.69$ mSv and $9.74{\pm}2.55$Sv for 30 male patients respectably $5.87{\pm}1.09$ mSv, $8.43{\pm}1.89$ mSv and $9.19{\pm}2.29$ mSv for female patients respectably. When divided three groups which were under 50 kg, 50~60 kg and over 60 kg respectably were 6.27 mSv, 7.67 mSv and 9.33 mSv respectably using CT-Expo V1.7, 5.62 mSv, 7.22 mSv and 8.91 mSv respectably using ImPACT v1.0. Weight and the effective dose coefficient analysis showed a very strong positive correlation(r=743, r=0.693). Conclusion: Using such a dose evaluation programs, easier to predict and evaluate the effective dose possible without performing phantom study and such dose evaluation programs could be used to collect basic data for CT dose management.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.4
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• pp.289-297
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• 1999

Concentrations of total dissolved free amino acids (DFAAs) in the northeast Pacific Ocean $9^{\circ}54'-10^{\circ}27'N$, $131^{\circ}43'-131^{\circ}53'W$) ranged from 15.9 to 1778.2 17M, and the average was 407.2 nM. Concentrations of DFAAs in surface mixed layers, ranged from 60.1 to 1411.9 nM, and the average was 535.2 nM. Seasonal thermoclines with maxima were formed between the depth of 50 to 150 m. DFAAs in this layer were varied in concentrations from 91.7 to 1778.2 nM, and the average was 588.5 nM. DFAAs below the seasonal thermoclines fluctuated from 15.9 to 384.2 nM, and the average was 175.1 nM. Consequently, in average relatively abundant DFAAs were observed in the subsurface layer than the deeper layer. DFAA vertical profiles and compositions of station A showed similar to station Band C. Glycine, alanine, glutamic acid, serine and valine were predominant accounting for more than 60% of total amino acids. Isoleucine, tyrosine, methionine and phenylalanine comprised only few percents of total DFAAs in the study area. In mole % of amino acid, according to characteristics of functional group of amino acid, aliphatic neutral accounted for 59% and aliphatic hydroxy 16%, acidic 12%, respectively. Although differences in DFAA concentrations with depth were observed, the amino acid composition and mole % of deeper layers in all stations were similar to those of subsurface layers. The results indicate that individual DtAAs remains invariably in water columns relative to the compositions and distributions of DFAAs in the study area, which may be the result of close coupling between microbial activity and their water solubility.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.2
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• pp.118-131
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• 1997

An opening efficiency experiment of anchovy boat seine has been carried out using a half size of the ordinary seine to reduce the size of the seine net and to improve the fishing efficiency from field operation. The intervals of towing boats were set at 100, 200 and 300m, and the towing speed, at 0.6, 0.9, and 1.2k't by possible combinations of them. The vertical openings of wing net, inside wing net, bagnet and flapper as well as the spreads of the seine net and the towing tension of the warp were measured, to find out efficient fishing gear and method of the anchovy boat seine. The results obtained are as follows : 1. The vertical opening of the inside wing net ranged from 8.7m to 12.0m at the normal current and from 7.0m to 10.0m at the counter current, and that of the wing net, from 8.4m to 19.8m at the normal current and from 4.9m to 16.3m at the counter current. The vertical opening of the wing net and inside wing net decreased as the towing speed and intervals of the boats increase, from 66% to 16% and from 32% to 18% of the normal opening, respectively. 2. The vertical opening of the fore of bag net ranged from 7.9m to 12.8m at the normal current and from 7.4m to 9.7m at the counter current, and that of the flapper, from 3.4m to 5.1m at the normal current and from 4.4m to 5.1m at the counter current, and that of the flapper, from 3.4m to 5.1m at the normal current and from 4.4m to 5.1m at the counter current. The vertical opening of the bag net was from 98% to 57% of the normal opening and the flapper showed a circular shape and it rose up to the upper layer with a slower towing speed. 3. The vertical opening of the end of the bag net ranged from 7.1m to 9.3m at the normal current and from 7.4m to 8.8m at the counter current. The end of the bag net rose up to the upper layer, This phenomenon was more apparent as the towing speed and the interval of the boats increase. 4. The towing tension of the experimental nets increased from 648kg to 2,716kg at the normal current and from 1,050kg to 6,010kg at the counter current with increasing towing speed. 5. The net depth of the anchovy seine was stable with the higher towing speed and the wider interval of the boats, but it was unstable by rising up to the upper layer with the lower towing speed and the narrower interval of towing boats.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.3
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• pp.151-161
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• 2016

This study was conducted to find out influence of dormancy level and carbon concentration on freezing hardiness in bourse shoot of 'Fuji' apple tree. Bourse shoot of 'Fuji' adult apple tree grafted on M.26 and M.9 rootstocks were used as experimental materials. Dormancy levels of bourse shoot were categorized according to the periods as follows the internal dormancy (late January), the early days after internal dormancy breaking (early February), the late days after internal dormancy breaking (late February), the bud break (late March), and the full bloom (late April). Chilling temperatures with bourse shoot were ranged from 0 to $-40^{\circ}C$. Also, the freezing hardiness according to carbon concentrations were investigated on 'Fuji'/M.9 apple tree that defoliated severely by Marssonina blotch (defoliation) and that of below the average 20 cm in shoot length through heavy crop load (weakness). Results showed that freezing hardiness of bourse shoot may become weaker after internal dormancy breaking. There was no differences in the carbon concentration of bourse shoot of 'Fuji' apple tree grafted on M.9 and M.26, so may be resulted in no difference in freezing hardiness both of bourse shoot grafted on M.9 and M.26 rootstock. Carbon concentration in bourse shoots with weakness and C/N ratio in bourse shoots with defoliation were lower than that of healthy. It may be shown that the freezing hardiness of defoliation and weakness were weaker than that of healthy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.914-919
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• 1998

This study was conducted to evaluate the purification efficacy by filter and ultra violet (UV) irradiation against inlet and outlet seawater of the land based fish farm. Purification efficacies for inlet seawater (50$\ell$/min) has been examined with filter of 60$\mu$m pore size and UV irradiation at an average dose 0.5 mWS/$cm^2$ for 5 months. For outlet seawater filter of 90 $\mu$m pore size only was used. Temperature, salinity, pH, $NO_3-N,\;NO_2-N,\;NH_4-N,\;PO_4-P$, DO and COD of inlet and outlet seawater in the system were not influenced. However, the removing rate of suspended solid and turbidity of inlet seawater were appeared to be $48.7\~65.6\%$ (average $51.9\%$) and $33/3\~42/5\%$ (average $34.8\%$) after passing through screen filter respectively. Also, germicidal efficiency to the total bacteris and Vibrio species were $16.7\~20.2\%$ (average $19.2\%$) $20.0\~21.9\%$ (average $20.9\%$) respectively after passing through UV irradiation. After passing through drum filter of 90$\mu$m pore size, suspended solid and turbidity of outlet seawater were appeared to be $42.7\~52.6\%$ (average $46.9\%$), $27.7\~29.9\%$ (average $28.3\%$), respectively.

• Development and Reproduction
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• v.14 no.3
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• pp.207-214
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• 2010

The tremendous changes of uterine endometrium are observed during early pregnancy and protease and their inhibitors are involved in regulation of cell proliferation and remodeling of the tissues through remodeling the extracellular matrix (ECM). Some of the proteases and protease inhibitors have been suspected to a factor in endometrial changes but many parts of their expression profiles and the physiological roles are not uncovered. To evaluate the functional roles of them, in this study the expression profiles of proteases and protease inhibitors were analyzed using real-time quantitative PCR analysis. Mmp9 (matrix metalloproteinase 9) mRNA levels peaked on day 4 at the time of implantation. On the other hand, Ela2 (neutrophil elastase, NE) mRNA levels were peaked on day 2 of pregnancy. Its expression were decreased until day 4 of pregnancy but increased rapidly until day 7 of pregnancy and decreased again. NE inhibitor Slpi (secretory leukocyte protease inhibitor, SLPI) mRNA levels were related with the implantation stage and with the levels of Ela2. At the time of implantation the expression levels of Slpi mRNA were about 5 times higher than the Ela2 mRNA in the uterus. In the implantation stage embryos, Mmp9 specific mRNA was only detected at the blastocyst. On the other hand, the expression level of SLPI was higher than that of the Ela2 mRNA at blastocyst and 4.5 day p.c. embryos. Based on these results it is suggested that MMP9, SLPI, and NE have important physiological role in embryo implantation both in uterus and embryos.

• Journal of the Korean Geosynthetics Society
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• v.11 no.3
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• pp.27-35
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• 2012

In this paper, the frost heaving and thawing characteristics of flexible pavement structure were evaluated in the large scale freezer which have a specification of temperature range $-20^{\circ}C{\sim}10^{\circ}C$ and $3.2m(L){\times}3.2m(B){\times}2.4m(H)$ in size. The insulated steel box with the size of $0.9m(L){\times}0.9m(B){\times}0.9m(H)$ was used to simulate actual pavement road structure. The variation of temperature, frost heave amount and frost heave pressure were measured through the instrument of TDS-602 data logger. LFWD (light falling weight deflectometer) was used to determine the change of deflection due to the frost heaving and thawing. Furthermore, the influence of aggregate layer to the freezing of the subgrade soil was studied to verify the function and effectiveness of the anti-freezing layer.

• Radiation Oncology Journal
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• v.31 no.3
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• pp.111-117
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• 2013

Purpose: To determine whether the maximum standardized uptake value (SUV) of [$^{18}F$] fluorodeoxyglucose uptake by positron emission tomography (FDG PET) ratio of lymph node to primary tumor (mSUVR) could be a prognostic factor for node positive non-small cell lung cancer (NSCLC) patients treated with definitive radiotherapy (RT). Materials and Methods: A total of 68 NSCLC T1-4, N1-3, M0 patients underwent FDG PET before RT. Optimal cutoff values of mSUVR were chosen based on overall survival (OS). Independent prognosticators were identified by Cox regression analysis. Results: The most significant cutoff value for mSUVR was 0.9 with respect to OS. Two-year OS was 17% for patients with mSUVR > 0.9 and 49% for those with mSUVR ${\leq}0.9$ (p = 0.01). In a multivariate analysis, including age, performance status, stage, use of chemotherapy, and mSUVR, only performance status (p = 0.05) and mSUVR > 0.9 (p = 0.05) were significant predictors of OS. Two-year OS for patients with both good performance (Eastern Cooperative Oncology Group [ECOG] ${\leq}1$) and mSUVR ${\leq}0.9$ was significantly better than that for patients with either poor performance (ECOG > 1) or mSUVR > 0.9, 23% (71% vs. 23%, p = 0.04). Conclusion: Our results suggested that the mSUVR was a strong prognostic factor among patients with lymph node positive NSCLC following RT. Addition of mSUVR to performance status identifies a subgroup at highest risk for death after RT.