• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.341-344
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• 1997

Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 1991.05a
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• pp.78-79
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• 1991

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.156-156
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.53-54
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• 1998

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.05a
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• pp.74-75
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• 2001

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2006.06a
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• pp.7.2-8
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• 2006

• Proceedings of the Korean Society of Grassland Science Conference
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• 2005.06a
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• pp.168-169
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• 2005

• Current Research on Agriculture and Life Sciences
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• v.21
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• pp.1-9
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• 2003

Plants are often subjected to periods of soil and atmospheric water deficit during their life cycle. To find critical levels for identification of tolerant rice variety to salt- and drought-stresses, we investigated the water deficiency in the leaf of a Dongjinbyeo (DJ) cultivar, identified as intolerant variety, subjected to NaCl- and Polyethylene glycol 6000 (PEG)- treatments. The relative water content and water potential in leaf of DJ plant sharply declined along the high concentration and time after treatment in NaCl- and PEG-treated rice plants. To elucidate the method of simple screening of tolerant variety to salt- and drought-stresses, we examined the relationship between relative water content and water potential of leaves in NaCl- and PEG-treated rice plants. The relationship between relative water content and water potential in leaf of DJ plant showed the highest correlation in 80 mM NaCl-treatment, and showed high correlation only 8% PEG treatment. These results indicate that the critical level of salt stress for screening of tolerant rice was 80 mM NaCl at 48 h after NaCl treatment, and the critical concentration of drought stress for screening of tolerant rice was 8% PEG at 96 h after PEG treatment.

• Korean Journal of Heritage: History & Science
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• v.42 no.3
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• pp.48-65
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• 2009

In order to understand the local vegetation history and the origin of rice cultivation, a phytoliths analysis was performed with sediments collected from archaeological site at Ga-dong in Gijang, Busan. From the analysis with the sediments of three sampling sites, fan-shape phytoliths of Oryza sativa were detected: sediment 8 ~ sediment 29 of sampling site 1, sediment 6 ~ sediment 20 of sampling site 2 and sediment 7 ~ sediment 33 of sampling site 3. On the bottom layers or near the bottom layers from the three sampling sites, high densities of Arundinoideae and Andropogoneae were detected. It indicates that these layers were wet soil environment when they were sedimented. From near bottoms up to surfaces, the densities of Arundinoideae and Andropogoneae were abruptly decreased, while the density of Oryza sativa was sharply increased. With these results, it should be considered that the rice cultivation was started from the Bronze Age on this region.

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.389-397
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• 2007

Using the active-increment of Tos17 copies in the genome of Oryza sativa L., there were many studies about induction and selection of new mutants. This study mainly focuses on the induction for retrotransposon(Tos17) activity in the callus of Ilpumbyeo(Oryza sativa L.) according to varied culture period and condition. The objectives of this study are obtaining various mutants($M_1$) through plant regeneration, identification of the mutation relation with Tos17, and subsequent phenotyping of the mutants($M_2,\;M_3$). A total of 371 $M_1$ mutants was obtained. The degree of Tos17 activity obtained regeneration plants with each different culture period was evaluated by Southern blot analysis. The result showed that control Ilpumbyeo rice has 5 numbers of copies and the band numbers obtained 7, 8, 9.5, 12, 6, 13.5, 17.5 from culture period of 1, 2, 3, 5, 6, 7, 8 month, respectively. In this study, the result showed that most effectual culture period for activity of Tos17 in Ilpumbyeo rice is 5 month. Hereafter, collections and analysis of various recombination plants will act on an important factor in multiplication and preservation of $M_2$ and $M_3$ generation. And an urgent and important subject is a development of screening method for selection of diverse mutants.