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The Studies of Activity of Retrotransposon(Tos17) according to Tissue Culture Periods in Rice(Oryza sativa L.)  

Yang, Hee-Eun (Department of Plant Biotechnology, Division of Biotechnology, School of Bioscience & Biotechnology, Kangwon National University)
Fang, Yilan (Department of Plant Biotechnology, Division of Biotechnology, School of Bioscience & Biotechnology, Kangwon National University)
Shin, Young-Boum (Department of Plant Biotechnology, Division of Biotechnology, School of Bioscience & Biotechnology, Kangwon National University)
Lee, Boung-Jin (Experimental Station, College of Agricultural and Life Sciences, Kangwon National University)
Hong, Soon-Kwan (Department of Plant Biotechnology, Division of Biotechnology, School of Bioscience & Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.20, no.5, 2007 , pp. 389-397 More about this Journal
Abstract
Using the active-increment of Tos17 copies in the genome of Oryza sativa L., there were many studies about induction and selection of new mutants. This study mainly focuses on the induction for retrotransposon(Tos17) activity in the callus of Ilpumbyeo(Oryza sativa L.) according to varied culture period and condition. The objectives of this study are obtaining various mutants($M_1$) through plant regeneration, identification of the mutation relation with Tos17, and subsequent phenotyping of the mutants($M_2,\;M_3$). A total of 371 $M_1$ mutants was obtained. The degree of Tos17 activity obtained regeneration plants with each different culture period was evaluated by Southern blot analysis. The result showed that control Ilpumbyeo rice has 5 numbers of copies and the band numbers obtained 7, 8, 9.5, 12, 6, 13.5, 17.5 from culture period of 1, 2, 3, 5, 6, 7, 8 month, respectively. In this study, the result showed that most effectual culture period for activity of Tos17 in Ilpumbyeo rice is 5 month. Hereafter, collections and analysis of various recombination plants will act on an important factor in multiplication and preservation of $M_2$ and $M_3$ generation. And an urgent and important subject is a development of screening method for selection of diverse mutants.
Keywords
Tos17; Southern blot; Tissue culture; Rice; Mutant; Retrotransposon;
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1 Hirochika H., K. Sugimoto, Y. Otsuki, H. Tsugawa and M. Kanda. 1996. Retrotransposons of rice involved in mutations induced by tissue culture. PNAS USA 93: 7783-7788   DOI
2 Marillonnet S. and S.R. Wessler. 1998. Extreme Structural Heterogeneity Among the Members of a Maize Retrotransposon Family. Genetics 150: 1245-1256
3 Murashige T. and F. Skoog. 1962. A Revised medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol. Plant 15: 473-497   DOI
4 Vicient C.M., A. Suoniemi, K.A. Jonsson, J. Tanskanen, A. Beharav, E. Nevo and A.H. Schulman. 1999. Retrotransposon BARE-1 and Its Role in Genome Evolution in the Genus Hordeum. Plant Cell 11: 1769-1784   DOI   ScienceOn
5 Wang S., N. Liu, K. Peng and Q. Zhang. 1999. The distribution and copy number of copia-like retrotransposons in rice (Oryza sativa L.) and their implications in the organization and evolution of the rice genome. PNAS USA 96: 6824-6828   DOI
6 Hirochika H. 1993. Activation of tobacco retrotransposons during tissue culture. EMBO J. 12: 2521-2528
7 Fukuchi A., F. Kikuchi and H. Hirochika. 1993. DNA fingerprintion of cultivated rice retrotransposon probes. Jpn, J. Genet. 68: 195-204   DOI
8 Hirochika H., A. Fukuchi and F. Kikuchi. 1992. Retrotransposon families in rice. Mol. Gen. Gemet. 233: 209-216   DOI
9 Xie Q.J., M.C. Rush and S.D. Linscombe. 1996. Inheritance of homozygous somaclonal variation in rice. Crop Sci. 36: 1491-1495   DOI
10 Hirochika H., H. Otsuki, M. Yoshikawa, Y. Otsuki, K. Sugimoto and S. Takeda. 1996. Autonomous transposition of the tobacco retrotransposon Ttol in rice. Plant Cell 8: 725-734   DOI   ScienceOn
11 Miyao A., K. Tanaka, K. Murata, H. Sawaki, S. Takeda, K. Abe, Y. Shinozuka, K. Onosato and H. Hirochika. 2003. Target site specificity of the Tos17 retrotranposon shows a preference for insertion within genes and against insertion in retrotranposon-rich regions of the genome. Plant Cell 15: 1771-1780   DOI
12 Grandbastien M.A., A. Spielman and M. Caboche. 1989. Tntl, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics. Nature 26: 376-380
13 Fuerstenberg S.I. and M.A. Johns. 1990. Distribution of Bs1 retrotransposons in Zea and related gene. Thero. Appl. Genet. 80: 680-686
14 Yi G.H., M.H. Nam, B.G. Oh, H.C. Choi, S.C. Kim, C.D. Han and J.K. Sohn. 1999. Activation of retrotransposon in plant variants derived from rice cell culture. Korean J. Breed. 31: 341-347
15 Yamazaki M., K. Tsugawa, A. Miyao and M. Yano. 2001. The rice retrotransposon Tos17 prefers low-copy-number sequences as integration targets. Mol. Genet. Genomics 265: 336-344   DOI
16 Kumar A. and J.L. Bennetzen. 1999. Plant Retrotransposons. Annu. Rev. 33: 479-532   DOI   ScienceOn
17 Blakely L.M. and F.C. Steward. 1964. Growth and organized development of cultured cells 7 cellular variation. Amer. J. Bot. 51(8): 809-820   DOI   ScienceOn
18 Hirochika H. 1997. Retrotransposons of rice : their regulation and use for genome analysis. Plant Mol. Biol. 35: 231-240   DOI   ScienceOn
19 Ruiz M.L., J. Rueda, M.I. Pelaez, F.J. Espono, M. Candela, A.M. Sendino and A.M. Vazquez. 1992. Somatic embryogenesis, plant regeneration and somaclonal variation in barley. Plant Cell, tissue and Organ Culture 28: 97-101   DOI
20 Moore G., H. Lucas, N. Batty and R. Flavell. 1991. A family of retrotransposons and associated genomic variation in wheat. Genomics 10: 461-468   DOI
21 Gabriel A., M. Willems, E.H. Mules and J.D. Boeke. 1996. Replication infidelity during a single cycle of Tyl retrotransposition. PNAS USA 93: 7767-7771   DOI
22 Manninen, I. and A.H. Schulman. 1993. BARE-1, a copoa-like retroelement in barley (Hordeum vulgare L.). Plant Mol. Biol. 22: 829-846   DOI
23 Larkin P.J. and W.R. Scowcroft. 1981. Somaclonal variation a novel source of variability from cell cultures for plant improvement, Theor. Appl. Genet. 60: 197-214   DOI   ScienceOn
24 Tai T.H. and S.D. Tanksley. 1990. A rapid and inexpensive method for isolation of total DNA from detydrated plant tissue. Plant Mol. Biol. Report 8(4): 297-303   DOI