Abstract
Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.
알팔파의 자엽과 하배축을 배양하여 캘러스를 유기하고 이들을 glyphosate첨가 액체배지에서 배양하여 glyphosate에 대한 생장억제 반응을 검사하였으며 10 mM glyphosate에 저항성인 세포주를 선발하고 EPSPS 활성을 조사 하였던 바 캘러스 유기율은 2,4-D 1 mg/L, kinetin 0.5 mg/L에서 가장 높았고 자엽보다 하배축이 캘러스 유기율이 높았으며, glyphosate 농도에 대한 ID$_{50}$은 0.1 mM과 0.2 mM사이에 있었다. A49-10G와 A58-10G는 대조세포주에 비해 각각 8.0배 와 9.1배의 높은 EPSPS 활성을 보였다.