• 생물환경조절학회지
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• 제26권3호
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• pp.227-234
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• 2017

본 연구는 딸기 '매향'의 양수분 흡수율을 고려하여 개발한 배양액을 검증하고자 이온 조성이 다른 5종 배양액으로 8개월 동안 수경재배하면서 생육과 수량에 미치는 영향을 조사하였다. 2015년 9월 22일 코이어 고설베드에 딸기 묘를 정식하고 1개월 후 다섯 종류의 배양액 농촌진흥청 딸기 배양액(RDA), 야마자키 딸기 배양액(Yamazaki), PBG 딸기 배양액(PBG), 서울시립대 딸기 배양액(UOS) 및 새로 개발된 서울시립대 딸기 배양액(NewUOS)을 사용하여 EC $1.0dS{\cdot}m^{-1}$, pH 6.0으로 1일 주당 150~300mL 공급하였다. 정식 60일 후 엽폭, 엽병장은 배양액 종류에 차이를 보였으며, 광합성은 RDA와 NewUOS 배양액 처리에서 높았고, PBG 배양액 처리에서 낮았다. 영양생장기 배액의 EC는 공급수준보다 낮은 EC $0.7{\sim}0.8dS{\cdot}m^{-1}$, 이후는 EC $1.0{\sim}1.2dS{\cdot}m^{-1}$로 안정되었다. 배액 pH는 Yamazaki 배양액 처리에서 6.2~6.8로 높은 반면, UOS 배양액은 5.1~5.2로 낮았다. 영양생장기 배액의 무기이온은 질산태 질소의 흡수가 가장 활발하였으며, 화방전개 이후 칼륨 흡수가 NewUOS, UOS 및 Yamazaki 배양액 처리에서 높았다. 정식 6개월 후 지상부와 지하부 생체중과 건물중은 UOS와 NewUOS 배양액에서 높았으며, 지상부 건물율은 Yamazaki 배양액에서 43.5%로 낮았으며, 지하부 건물율은 NewUOS배양액에서 30.6%로 낮았다. 12월부터 2월까지 수확된 딸기의 과장, 과폭, 과중, 당도는 배양액 차이에 의한 유의성은 없으나, NewUOS 배양액에서는 주당 과수와 평균 과중이 높아 수량이 높았다. 3월부터 5월까지 Yamazaki 배양액에서 수확된 딸기는 주당 과수와 수량이 높았다. 따라서 이온 조성 차이에 따른 배양액 5종으로 수경재배하였을 때 '매향'의 생육은 차이를 보이지 않았으나, 시기별 상품성 향상을 위해 정식 후 ~ 2월까지는 NewUOS 배양액을, 고온과 화방당 착과량이 많아지는 3월 이후에는 Yamazaki 배양액으로 재배하는 것이 적합하리라 본다.

• 한국공간구조학회논문집
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• 제1권1호
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• pp.95-108
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• 2001

A system of equations is developed for the theory of bending of thick orthotropic elastic plates which takes into account the transverse shear deformability of the plate. This system of equations is of such nature that three boundary conditions can and must be prescribed along the edge of the plate, i.e. ${\omega}=0,\;M_x=0,\;M_{xy}=0\;({\omega}=0,\;M_x=0,\;M_{xy}=0)$ at simple supported edges. It can be obtained general solution that is added complementary solution ${\omega}^e$ and paticular solution ${\omega}^p$ by an assumption of solution function. In the next paper, this analytical results will be obtained for perforated thick plates.

• Reproductive and Developmental Biology
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• 제31권2호
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• pp.103-108
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• 2007

This study was carried out to investigate the effects of cryoprotectants, warming solution and removal of lipid on open pulled straw vitrification (OPS) method of porcine embryos produced by nuclear transfer (NT) of fetal fibroblasts. All solutions used during vitrification were prepared with holding medium consisting of 25 mM Hepes buffered TCM199 medium containing 20% fetal bovine serum (FBS) at $38.5^{\circ}C$. The blastocysts derived from NT with or without lipid were vitrified in each medium of different concentrations of dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Also, blastocysts after cryopreservation were warmed into different concentrations of sucrose in warming solution. The optimal concentrations of cryoprotectants in vitrification solution were 10% DMSO + 10% EG in vitrification solution 1 (VS1) and 20% DMSO + 20% EG in vitrification solution 2 (VS2). The optimal concentrations of sucrose were 0.3 M sucrose in warming solution 1 (WS1) and 0.15 M sucrose in warming solution 2 (WS2). lipid removal from oocytes before NT enhanced the viability of NT embryos after vitrification. Our results show that use of the OPS method in conjunction with lipid removal provides effective cryopreservation of porcine nuclear transfer embryos.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.113-113
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• 2003

This study was conducted to investigate the effect of vitrification solution(VS) on in vitro developmental competence of immature porcine oocytes. The immature porcine oocytes were exposed to the following vitrification solution, at RT. 1) EFS sol. : 20% ethylene glycol (EG) 3 min, 40% EG + 18%(w/v) Ficoll(MV70, 000) + 0.3 M sucrose 30 sec, 2) GE sol. : 10% glycerol 5 min, 10% G + 20% EG 5 min, 25% G +25% EG 30 sec, 3) EG sol : 1.5M EG 2.5 min, 5.5 M EG + 1.0 M sucrose 30 sec. Oocytes were immediately transferred into 1.0 M, 0.5 M, 0.25 M, 0125 M, 0 M sucrose solution for 2.5 min each at RT. After removal of VS, immature oocytes were matured in vitro and subsequently all oocytes were subjected to IVF followed in vitro culture for 7 days. Maturation rates of oocytes were 38.8%, 44.5%, 22.4% and 57.6%, in EFS, EG, GE and Control, respectively, maturation rates of oocytes in EG and Control was significantly higher than EFS and GE(P<0.01). Fertilization rates of oocytes in Control was significantly higher than other treated groups(P<0.05), but no difference were observed among treated groups. Polyspermic rates were no significant difference among four groups. Cleavage rates of oocytes were 21.9%, 47.1%, 19.0% and 65.9%, in EFS, EG, GE and Control, respectively, cleavage rates of oocytes in EG and Control was significantly higher than EFS and GE(P<0.05), but blastocyst formation rates were no significant difference among four groups. These results suggested that the use of EG solution could be a great challenge for reaching a successful vitrification of immature porcine oocytes.

• The Korean Journal of Physiology
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• 제20권2호
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• pp.184-191
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• 1986

Membrane potential of cells in the isolated rabbit coronary sinus was measured by conventional glass microelectrode and investigated the effect of $[K^+]_0$ variation in control, 20 mM and Ach-containing Tyrode solution. The results obtained were as follows: 1) The resting membrane potential exposed to normal Tyrode solution containing 3 mM $K^+\;was\;about\;-60{\sim}\;-65mV$. At extracellular $K^+$ concentrations from 1 to 30 mM the resting Potential was reasonably well described by Goldman -Hodgkin -Katz equation on the assumption that $[K^+]_1$ was 150 mM and that the ratio of membrane permeability coefficient for $Na^+\;and\;K^+,\;P_{Na}/P_K\;({\alpha})$ was 0.07. 2) In 20 mM Na-Tyrode solution (replacing by equimolar Tris) the resting membrane potential was hyperpolarized by 15 to 20 mV and showed slightly deviated to depolarized direction compared to the predicted value by Goldman-Hodgkin -Katz equation. 3) In the presence of $10^{-6}M$ Ach, the resting potentials at $[K^+]_0$ levels from 1 to 30 mM were well fitted with the predicted value on the assumption that $P_{Na}/P_K$ was 0.0144. It could be concluded that the low resting membrane potential of coronary sinus cells reflects a relatively high ratio $P_{Na}/P_K$ of about 0.07.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.380-383
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• 2003

In this paper, we propose a fuzzy group decision making method for multiple decision maker-multiple objective programming problems to obtain the agreeable solution. In the proposed method, considering the vague nature of human subjective judgement it is assumed that each of multiple decision makers has a fuzzy goal for each of his/her own objective functions. After eliciting the membership functions from the decision makers for their fuzzy goals, total M-Pareto optimal solution concept is defined in membership spaces in order to deal with multiple decision maker-multiple objective programming problems. For generating a candidate of the agreeable solution which is total M-Pareto optimal, the extended weighted minimax problem is formulated and solved for some weighting vector which is specified by the decision makers in their subjective manner, Given the total M-Pareto optimal solution, each of the derision makers must either be satisfied with the current values of the membership functions, or update his/her weighting vector, However, in general, it seems to be very difficult to find the agreeable solution with which all of the decision makers are satisfied perfectly because of the conflicts between their membership functions. In the proposed method, each of the decision makers is requested to estimate the degree of satisfaction for the candidate of the agreeable solution. Using the estimated values or satisfaction of each of the decision makers, the core concept is desnfied, which is a set of undominated candidates. The interactive algorithm is developed to obtain the agreeable solution which satisfies core conditions.

• Corrosion Science and Technology
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• 제20권2호
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• pp.77-84
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• 2021

This study aims to optimize the DL-EPR test solution for duplex stainless steel S31083 using the Taguchi design. The test solution parameters applied to the Taguchi design are H₂SO₄, NaCl, KSCN concentration, and temperature. In the experimental design, an orthogonal array of 4 levels 4 factor L₁₆(4⁴) was used. Output values for the orthogonal array were used for resolution (degree of sensitization) and selective etch (I_a) values. The optimal test solution conditions were selected by comparing the normalized S/N ratio for the two reaction properties. As a result, the H₂SO₄ and NaCl were identified as the main factors influencing the sensitivity measurement, but the delta statistics showed that the KSCN concentration and temperature had relatively low influence. The optimal condition was identified as 1.5 M H₂SO₄+0.03 M KSCN+1.5M NaCl at 30 ℃. The degree of sensitization presented a tendency to depend on the heat treatment temperature and time in the optimal test solution. This investigation confirmed the possibility of optimizing the experiment solution for the DL-EPR test of stainless steel using the Taguchi technique.

• 한국지하수토양환경학회지:지하수토양환경
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• 제21권6호
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• pp.146-155
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• 2016

The field feasibility tests for a phytoextraction process were performed to identify the effect of citric acid as a chelate on the uranium (U) transfer into the plant for the naturally U contaminated soil in Duckpyeongri, Korea. For the feasibility tests, lettuce and Chinese cabbage were cultivated for 49 days on four testing grounds ($1m{\times}1m{\times}0.5m$ in each) in 2016. The citric acid solution was added to two testing grounds (one for lettuce and the other for Chinese cabbage) increasing the U transfer in two crop plants and their results were compared to those without the citric acid solution. When without the citric acid solution, the U concentration of plant after the cultivation was low (< $45{\mu}g/kg$ for leaves and < $450{\mu}g/kg$ for roots). However, with the addition of 50 mM citric acid solution, the U concentration of lettuce leaves and roots increased by 24 times and 1.8 times, and the U concentration of Chinese cabbage leaves and roots increased by 86.7 times and 5.4 times. The absolute accumulated U amount (${\mu}g$) in lettuce and Chinese cabbage also increased by 8.7 times and 50 times, compared to those without citric acid solution. Less than 8% of the U amount of exchangeable/carbonate phases was removed by using the lettuce and Chinese cabbage when the citric acid solution was not applied. However 52% and 66% of the U amount in exchangeable/carbonate phases were removed by the lettuce and the Chinese cabbage when the citric acid solution was added. The effect of the citric acid on the U transfer capability into the plants was quantitatively investigated by the field feasibility test, suggesting that U existing as exchangeable/carbonate phase in soil can be successfully removed by the phytoextraction process using Chinese cabbage with citric acid.

• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.91-97
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• 2017

Hyperglycemia is associated with an increased risk of cardiovascular diseases. It has been demonstrated that chronic exposure to high glucose impaired endothelial functions. However, specific effects of short-term exposure to high glucose on vascular reactivity are controversial. Moreover, the combined effects of other metabolic substrates such as free fatty acids (FFA) on vascular reactivity remain poorly understood. Here we investigate the effects of short-term exposure to high glucose with or without other metabolic substrates including FFAs termed "nutrition full" (NF) solution, on mesenteric (MA) and deep femoral arteries (DFA) of rats. Arterial ring segments were mounted in a double-wire myograph. Contraction in response to phenylephrine (PhE) was determined in control (5 mM) and high glucose (23 mM, HG) environments over a 30 min period. In both arteries, PhE-inducedvasocontraction was enhanced by pre-incubation of HG solution. A combined incubation with HG and palmitic acid ($100{\mu}M$) induced similar sensitization of PhE-contractions in both arteries. In contrast, high $K^+$-induced contractions were not affected by HG. Interestingly, pre-incubation with NF solution decreased PhE-induced contraction in MA but increased the contraction in DFA. In NF solution, the HG-induced facilitation of PhE-contraction was not observed in MA. Furthermore, the PhE-induced contraction of DFA was attenuated by HG in NF solution. Our results demonstrate that the sensitization of PhE-induced arterial contraction by HG is differentially affected by other metabolic substrates. The conversation of skeletal arterial contractility by HG in NF solution requires careful interpretation of the previous in vitro studies where only glucose is included in physiological salt solutions. Further studies are required to elucidate the mechanism underlying the inconsistent effect of NF solution on MA and DFA.

• 공업화학
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• 제18권4호
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• pp.391-395
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• 2007

본 연구는 electrokinetic process (EK process)를 이용하여 20000 mg/kg의 납(Pb)으로 오염된 토양을 처리하는 과정에서 중금속이 오염토양 내부에 수산화물 형태로 침전되는 현상을 억제할 수 있는 방법을 찾기 위하여 전극교환(EK-Exchange, EK-E)과 혼합용매(0.3 M acetic acid and 0.03 M EDTA, EK-Mixed Solution, EK-M)를 사용하는 방법을 적용하였다. 혼합용매를 사용하는 경우(EK-M)에는 EDTA 0.03 M을 단독으로 사용하는 경우(EK-Blank, EK-B)보다는 약 2% 정도 낮은 제거효율을 나타내었다. 그러나, 비싼 EDTA 약품 값을 절약할 수 있다는 측면에서 보면, 이 방법도 충분히 적용 가능한 방법이다. 전극교환을 하는 경우(EK-E)에는 EDTA 0.03 M을 사용하는 경우(EK-B)보다 2% 높은 제거효율을 나타내었다. 그러나 더욱 중요한 사항은 다른 운전조건(EK-B, EK-M)과는 달리 토양 내부의 pH가 7~8 사이로 중성 부근에서 유지되었다는 점이다. 특히, 중금속의 축적이 우려되는 EK-B와 EK-M과는 달리 중금속이 특정 부위(오염토양 중앙부 또는 음극부)에 축적되지 않는다는 장점을 나타내었다.