• Journal of the Korean Chemical Society
• /
• v.38 no.10
• /
• pp.769-773
• /
• 1994

Higher order structure of 5S rRNA isolated from Xanthomonas celebensis was examined using angiogenic extracted from milk. Angiogenin cleaved exclusively 3' P-O bonds on the far sides of pyrimidines in the single-stranded sequences of 5S rRNA. Whereas angiogenin acted only on the loop d of 5S rRNA at pH 7.0 in the presence of 10 mM $Mg^{2+}$, it acted on all the loops (a, b, c and d) except loop e in the absence of $Mg^{2+}$. In the absence of $Mg^{2+}$, bonds $U_{74}$-$G_{75}$, $U_{77}$-$A_{78}$ and $U_{103}$-$A_{104}$ were highly susceptible to the action of angiogenin both at pH 7.0 and at pH 3.5. On the other hand, at pH 3.5 in the absence of $Mg^{2+}$ angiogenin strongly cleaved the bond $C_{17}$-$G_{18}$ of loop a and the bond $U_{55}$-$G_{56}$ of loop b. The results lead us to the following conclusion. First, angiogenin can be used as one of the probes for the tertiary structure analysis of 5S rRNA. Second, the structure of loop d of 5S rRNA is variable depending on the concentrations of $Mg^{2+}$ and $H^{1+}$.

• Journal of the Korean Chemical Society
• /
• v.37 no.2
• /
• pp.237-243
• /
• 1993

The primary and secondary structure of the 5S rRNA isolated from Xanthomonas celebensis were determined by enzymatic and chemical degradation methods. It consists of 119 nucleotides and contains no modified nucleosides. As with the 5S rRNAs of X. maltophilia and X. citri, it contains an additional uridine residue on the 5'-terminus. Its secondary structure was almost identical to the models previously proposed by us for the 5S rRNA of two Xanthomonas species. Its secondary structure consists of five helices, five loops and two bulges. The tertiary interactions in the 5S rRNA molecule were analyzed by Fe(II)-EDTA treatment and hybridization method using deoxyhexamer. From the fact that some adenine residues in loop M, region $I_1-C$, loop $H_1$, and loop $H_2$ become susceptible to diethylpyrocarbonate when the 5S rRNA was hybridized with deoxyhexamer complementary to the sequence $U_{35}CCCAU_{40}$ and that some nucleotide residues in loop M, loop $H_1$ and region $D-I_2$ become resistant Fe(II)-EDTA cleavage in the presence of $Mg^{2+}$, it is presumed that loops $H_1$ and $H_2$ interact with loop M in some way. In the tertiary interaction, the regions $I_1-C$ and $D-I_2$ seem to act as hinges in folding the stems $B-I_1-C$ and $D-I_2-E.$ It was found that loop $H_1$ changes into a smaller loop of three bases by forming noncanonical A : C base-pairs ih acidic environment.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.12
• /
• pp.1688-1695
• /
• 2016

The maternally inherited mitochondrial DNA (mtDNA) D-loop region is widely used for exploring genetic relationships and for investigating the origin of various animal species. Currently, domestic ducks play an important role in animal protein supply. In this study, partial mtDNA D-loop sequences were obtained from 145 samples belonging to six South-East Asian duck populations and commercial duck population. All these populations were closely related to the mallard duck (Anas platyrhynchos), as indicated by their mean overall genetic distance. Sixteen nucleotide substitutions were identified in sequence analyses allowing the distinction of 28 haplotypes. Around 42.76% of the duck sequences were classified as Hap_02, which completely matched with Anas platyrhynchos duck species. The neighbor-joining phylogenetic tree also revealed that South-East Asian duck populations were closely related to Anas platyrhynchos. Network profiles were also traced using the 28 haplotypes. Overall, results showed that those duck populations D-loop haplotypes were shared between several duck breeds from Korea and Bangladesh sub continental regions. Therefore, these results confirmed that South-East Asian domestic duck populations have been domesticated from Anas platyrhynchos duck as the maternal origins.

• Journal of the Korean Magnetics Society
• /
• v.4 no.2
• /
• pp.89-93
• /
• 1994

Effects of density, inner-stress, and grain size on B-H hysteresis loop properties of Mg-ferrite were investigated. As the sintered bulk density increase, coercive force($H_c$) decreasand squareness ratio increase. Coercive force was very dependent on inner-stress in sintered body, so coercive force increase from 1.95[Oe] to 4.35[Oe] when inner stress present in bulk, however, the squareness ratio was almost not changed Coercive force and squareness ratio were independent on grain size of sintered body which is between 6-11[$\mu\textrm{m}$]

• Korean Journal of Veterinary Service
• /
• v.40 no.1
• /
• pp.15-20
• /
• 2017

A two-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was designed for the rapid visual detection of the M gene of all subtypes of avian influenza virus (AIV) and the H5 gene of the H5 subtype of highly pathogenic AIV (HPAIV). The reaction carried out in two tubes in a single step at $58^{\circ}C$ for 40 min, and the assay results could be visually detected by using hydroxynaphthol blue dye. Using M or H5 gene-specific primers, the assay successfully detected all subtypes or H5 subtypes of AIVs, including the Korean representative H5N1 and H5N8 HPAIVs. The detection limit of the assay was approximately $10^{2.0}$ $EID_{50}/reaction$ for the M and H5 genes of H5N1 HPAIV, respectively, and was more sensitive than that of previously reported RT-LAMP and comparable to that of real-time RT-PCR. These results suggest that the present RT-LAMP assay, with its high specificity, sensitivity, and simplicity, will be a useful diagnostic tool for surveillance of currently circulating H5 HPAIVs and other subtypes of AIV in bird population, even in under-equipped laboratories.

• Journal of the Korean Magnetics Society
• /
• v.5 no.5
• /
• pp.900-904
• /
• 1995

We have developed a new torque magnetometric method for measuring the M-H hysteresis loop of a spheroid-shape magnetic material having a uniaxial anisotropy. Our torque magnetometric method gives the saturation magnetization as well as the remnant magnetization, simultaneously. A torque magnetometer having the torque sensitivity of $10^{-6}$ dyn cm could give the extreamly high sensitivity of $10^{-9}$ emu in measuring the magnetic moment, which is high enough to measure the magnetic moment of a monolayer Ni film. The accuracy of the present method was negligibly affected even at the applied fields near the coercivity where magnetization was inhomogeneous.

• Journal of the Korean Magnetics Society
• /
• v.5 no.5
• /
• pp.387-390
• /
• 1995

We have developed a new method for measuring the M-H hysteresis loop of a spheroid-shape magnetic material having a uniaxial anisotropy and discussed its accuracy at fields near the coercivity. Our torque magnetometric method simultaneously gives the saturation magnetization and the remnant magnetization. Furthermore, the coercivity depending on the applied field orientation is accurately measured by this simple technique. An accuracy of the present method is negligibly affected even at fields near the coercivity, where the magnetization is not uniform. The technique makes a torque magnetometer an extremely high sensitive tool for measuring M-H hysteresis loop.

• Proceedings of the KIEE Conference
• /
• 1999.07b
• /
• pp.586-588
• /
• 1999

A robust controller design methodology is proposed, where the proposed controller consists of a servo controller at the outer loop as well as the robust internal-loop compensator (RIC) to eliminate the model uncertainty and external disturbance. It is shown that RIC is general structure of DOB and ARC. Numerical example is illustrated to show validities of the proposed robust controller.

• Proceedings of the Korean Nuclear Society Conference
• /
• 2004.10a
• /
• pp.1373-1374
• /
• 2004

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.51-54
• /
• 2002

When a cold HPSI (High Pressure Safety Injection) fluid associated with an design basis accident, such as LOCA (Loss of Coolant Accident), enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena will arise due to incomplete mixing. If the stratified flow enters a reactor pressure vessel downcomer, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. Previous thermal-mixing analyses have assumed that the thermal stratification phenomena generated in stagnated loop of a partially stagnated coolant loop are neutralized in the vessel downcomer by strong flow from unstagnated loop. On the basis of these reasons, this paper presents the thermal-mixing analysis results in order to identify the fact that the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is affected by the strong flow of the unstagnated loop.