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Clinical Significance of Pyuria in Pediatric Patients with Febrile Urinary Tract Infection (발열성 소아 요로감염에서 농뇨의 임상적 의의)

  • Choi, Lim;Cho, Sea-Eun;Yim, Hyung-Eun;Yoo, Kee-Hwan;Hong, Young-Sook;Lee, Joo-Won
    • Childhood Kidney Diseases
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    • v.15 no.1
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    • pp.66-75
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    • 2011
  • Purpose: Early diagnosis and treatment of febrile urinary tract infection (UTI) in children is important to prevent kidney damage. This study aims to evaluate the relationship between the presence of pyuria, the severity, and underlying genitourinary anomalies in patients with UTI. Methods: We retrospectively reviewed 293 patients with febrile UTI who were admitted to Korea University Guro Hospital during the period from June, 2007 until January, 2010. We divided the patients into two groups, one with the finding of pyuria at admission, and the other without, and compared the fever duration, white blood cell counts (WBC) and C-reactive protein (CRP) in peripheral bloods, hydronephrosis, cortical defects, vesicoureteral reflux and admission period. Results: Among the 293 patients with febrile UTI, 189 patients showed findings of pyuria whereas 104 patients did not. Patients with pyuria showed an increment of WBC ($14,694{\pm}485.2$ vs. $11,374{\pm}451.2/uL$, P <0.05) and CRP ($46.9{\pm}3.9$ vs $17.1{\pm}3.6$ mg/L, P <0.05) in peripheral blood sample. The presence of cortical defects (21.7 Vs 5.8%, P <0.05) and vesicoureteral reflux (15.9 Vs 6.7%, P <0.05) was also increased in patients with pyuria compared to patients without pyuria. There were no specific differences in fever duration, admission period, and hydronephrosis. Within the group with pyuria, CRP in peripheral blood sample increased proportionally with the increment of pyuria (P <0.05). Conclusion: In patients with febrile UTI, the increment of WBC in the urine sample can be a helpful predictor for increased CRP in peripheral blood and acute pyelonephritis.

Studies on mulberry tree years and mulberry fruit yield and mulberry popcorn disease and sales price (뽕나무 수령 및 오디 생산성, 오디균핵병 및 가격비교 연구)

  • Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Ji, Sang Duk;Kim, Nam Sook
    • Journal of Sericultural and Entomological Science
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    • v.53 no.1
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    • pp.19-28
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    • 2015
  • Though mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a new income crop. But, a stable production of mulberry was issued on damage by the weather disaster, so it is requires that agricultural disaster insurance import. So, in this study as a basic research for a importation of agricultural disaster insurance, the cultivation of mulberry for the production of mulberry fruits, the shape of tree, the number of a bearing fruit from each cultivar, the occurrence of mulberry fruit sclerotic disease, and a yield and the market price of mulberry fruits were investigated and that results obtained were as follows; The number of mulberry tree each 10a was abundant in Buan and Jeongeup region that cultivated a suitable cultivar on low cut shape, however, a large cultivated Gochang region was little. The number of a bearing fruit per 1m on the branch was highest in the variety of Cheongilppong at Gochang. Yield of mulberry fruits each 10a was Cheongilppong at Gochang, followed by Iksuppong at Sangju. Average sale price of the mulberry fruits was high in the order of Gwasang No. 2, Suwonppong, Iksuppong, and Cheongilppong.

Relationship between Fertilizer Application Level and Soil Chemical Properties for Strawberry Cultivation under Greenhouse in Chungnam Province (충남지역 시설 딸기재배지 시비수준과 토양 화학성과의 관계)

  • Choi, Moon-Tae;Lee, Jin-Il;Yun, Yeo-Uk;Lee, Jong-Eun;Lee, Bong-Chun;Yang, Euy-Seog;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.153-159
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    • 2010
  • Nowadays, Korean farmers rely more on chemical fertilizers than low input sustainable agriculture drawn from the farm itself. In order to improve soil nutritional imbalance for environment friendly agriculture in greenhouse, we have carried out a relationship between fertilizer application level, and soil chemical properties for strawberry cultivation at 56 sites in Chungnam Province. Average amount of nitrogen as basal fertilization was 92.3 Mg $ha^{-1}$ which higher 2.6 times compared to standard amount of basal fertilizer. In case of compost application more than 30 Mg $ha^{-1}$, excessive ratio compared to optimum level was higher 1.8 times for EC value, 3.0 times for available phosphate, 2.6 times for exchangeable potassium, 1.7 times for exchangeable calcium, and 1.6 times for exchangeable magnesium, respectively. Amounts of compost application significantly correlated with available phosphate (r=0.370, $p{\leq}0.01$), exchangeable potassium(r=0.429, $p{\leq}0.01$), exchangeable calcium(r=0.404, $p{\leq}0.01$), exchangeable magnesium(r=0.453, $p{\leq}0.01$), and exchangeable sodium(r=0.369, $p{\leq}0.01$), respectively. Our results suggest that soil nutrients management for sustainable agriculture was optimum fertilization based on soil testing for strawberry cultivation in greenhouse.

Lung Preservation Study for Above 20 Hours of LPDG Solution in Canine Lung Allotransplactation (폐이식 실험견에서 LPDG용액을 이용한 20시간 이상 폐보존효과 관찰)

  • Park, Chang-Gwon;Gwon, Geon-Yeong;Yu, Yeong-Seon
    • Journal of Chest Surgery
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    • v.30 no.10
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    • pp.949-960
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    • 1997
  • Background. Limited ischemic tolerance of the lung has remained one of the factors that limits the expansion of pulmonary transplantation as a treatment for end-stage pulmonary disease. Numerous studies on safe long term preservation for lung transplantation has been performed for the purpose of developing ideal preservation solution with extracellular type or intracellular type solutions. In this. study, we examined the efficacy of L DG solution in lung preservation longer than 20 hours by comparison with modified Euro-Collins solution. Iwethods. Thirty-(our adult mongrel dogs were divided into two groups. Donor lungs were flushed with LPDG solution(n=9) or modified Euro-Collins(MEC) solution(n=8) and stored for 24 hours at 1$0^{\circ}C$. All donor lungs were perfused through the pulmonary arteries with solutions containing prostaglandin El and verapamil. Left canine lung allotransplantations wereperformed. Assessment(hemodynamic indices and arterial blood gas analysis) of left implanted lung was made by occluding the right pulmonary artery for ten minutes using pulmonary artery Cuff. Assessment was repeated at the interval of 30 minutes, one hour, and two hours later after reperfusion and then chest X-ray, computed tomogram and lung perfusion scan were obtained. In survival dogs follow-up studies were done with assessment with chest X-ray, computed tomogram of the chest and lung perfusion scan on 7th day postoperatively. After preservation above 20 hours, pathological examinations for ultrastructural findings on right lung were performed in each group. Results. With respect to arterial oxygen tension, LPDG group was superior to MEC but there was no statistical significance for 2 hours after reperfusion. Mean pulmonary artery pressure was less increased(p < 0.05) and cardiac output higher(p <0.05) than MEC group until 2 hours after reperfusion. After 2 hours of reperfusion, both groups showed transplanted lung function deteriorated gradually. Perfusion scan of the transplanted lung in LPDG group showed better perfusion rate in immediate post-reperfusion, 3 days and 7 days later respectively but there was no statistical significance and corelation with PaO2 and computed tomoRravhic views. In scanning electron microscopy of pulmonary artery after preservation, LPDG group relatively shows less irregular protrusion of the inner surface of endothelial cell of poulmonary artery than MEC group. Conclusions, e concluded that LPDG solution can offer safe lung preservation above 20 hours with adequate immunosuppressive therapy and prevention of the infection.

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Grain Yield Potential of a Low-Tillering Large Panicle Type in Rice (벼 소얼 수중형 초형의 수량성)

  • Kim, Je-Kyu;B.S., Vergara
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.4
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    • pp.361-371
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    • 1992
  • For the increase of grain yield potential in rice, a low-tillering large panicled type has been suggested as an ideotype. A low-tillering plant type may have different yield potential and needs different cultural practices from that used in a high-tillering type for the maximum yield. This study was conducted to evaluate the grain yield performances of a low-tillering large panicled rice and high-tillering small panicled rice at different plant spacings, nitrogen(N) levels and seedling numbers per hill. A low-tillering large panicled genotype, IR25588 was compared with a high-tillering small panicled IR58. The grain yield of IR25588 was significantly higher than that of IR58 under a narrow spacing with high N level. The maximum yields of IR58 and IR25588 were reached at about 35,000 and 40,000 spikelets per m$^2$, respectively. The increased grain yield in IR25588 was mainly due to the increase in spikelet number per unit area which is the most precise indicator of grain yield in rice. The optimum spacing for the maximum yield was denser for IR25588 than that for IR58 under high N level. The intra-hill competition of the low-tillering type was lower than that of the high-tillering type. The higher dry matter production and bigger leaf area and culm weight were the main factors for increased grain yield in a low-tillering panicle weight type. Based on the results, the yield potential of a low-tillering panicle weight type was higher than that of a high-tillering panicle number type, especially under a close spacing with high N level.

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Effect of Silicate-Coated Rice Seed on Healthy Seedling Development and Bakanae Disease Reduction when Raising Rice in Seed Boxes (벼 상자육묘에서 규산코팅볍씨의 건묘육성과 벼키다리병 경감효과)

  • Kang, Yang-Soon;Kim, Wan Jung;Roh, Jae-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.1
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    • pp.1-8
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    • 2017
  • We investigated the effect of silicate coating of rice seeds on bakanae disease incidence and the quality of seedlings raised in seedling boxes and transplanted into pots. The silicate-coated rice seed (SCS) was prepared as follows. Naturally infested rice seeds not previously subjected to any fungicidal treatment were dressed with a mixture of 25% silicic acid at pH 11 and 300-mesh zeolite powder at a ratio of 50 g dry seed - 9 mL silicic acid - 25 g zeolite powder. The following nursery conditions were provided : Early sowing, dense seeding in a glass house with mulching overnight and no artificial heating, which were the ideal conditions for determining the effect on the seed. The nursery plants were evaluated for Gibberella. fujikuroi infection or to determine the recovery to normal growth of infected nursery plants in the Wagner pot. Seedlings emerged 2-3 days earlier for the SCS than they did for the non-SCS control, while damping-off and bakanae disease incidence were remarkably reduced. Specifically, bakanae disease incidence in the SCS was limited to only 7.8% for 80 days after sowing, as compared to 91.6% of the non-SCS control. For the 45-days-old SCS nursery seedlings, the fresh weight was increased by 11% and was two times heavier, with only mild damage compared to that observed for non-SCS. Even after transplanting, SCS treatment contributed to a lower incidence of further infections and possibly to recovery of the seedlings to normal growth as compared to that observed in symptomatic plants in the pot. The active pathogenic macro-conidia and micro-conidia were considerably lower in the soil, root, and seedling sheath base of the SCS. In particular, the underdeveloped macro-conidia with straight oblong shape without intact septum were isolated in the SCS ; this phenotype is likely to be at a comparative etiological disadvantage when compared to that of typical active macro-conidia, which are slightly sickle-shaped with 3-7 intact septa. A active intact conidia with high inoculum potential were rarely observed in the tissue of the seedlings treated only in the SCS. We propose that promising result was likely achieved via inhibition of the development of intact pathogenic conidia, in concert with the aerobic, acidic conditions induced by the physiochemical characteristics associated with the air porosity of zeolite, alkalinity of silicate and the seed husk as a carbon source. In addition, the resistance of the healthy plants to pathogenic conidia was also important factor.

Effect of Ad libitum and Restricted Milk Feeding on Growth Performance of Holstein Calves (제한 및 무제한 포유방법이 홀스타인 송아지의 성장능력에 미치는 영향)

  • Khan, M. A.
    • Journal of Animal Science and Technology
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    • v.48 no.6
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    • pp.871-880
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    • 2006
  • This study was conducted to estimate the maximum amount and time of milk consumption by Holstein calves during two months of pre- weaning period and to compare ad libitum milk feeding with conventional system for their effects on dry feed consumption, nutrient intake, growth performance and occurrence of diarrhea during the pre-weaning phase. Holstein calves (n=20) were alternatively fed colostrum at 10% of their body weight or ad libitum for the first three days (d) and then assigned either to conventional (n=10; calves were fed milk at 10% of their body weight from birth to weaning at d 60 of their age) or ad libitum milk feeding (n=10; calves were fed ad libitum milk from birth to weaning at d 60 of their age). Milk intake as a percent of body weight was increased in ad libitum fed calves from 13.59% at d 1 to 23.50% at d 30 of their age, thereafter it was gradually reduced. Ad libitum fed calves consumed daily 9.8kg milk compared with 4.3kg milk in restricted fed calves(p<0.05). Daily starter and mixed grass hay intake was gradually increased in both groups with the age however; the group differences were noted non-significant up to 40 d of age. Overall from birth to weaning conventionally fed calves ate 18.4, 20 and 18.9% more dry matter, crude protein and total digestible nutrients, respectively than ad libitum milk fed calves (p<0.05). Weight gain was 49, 18.4 and 26% higher in ad libitum milk fed calves than conventionally fed calves during the first month, the second month and from birth to weaning, respectively(p<0.05). Body weight was not significantly different between groups for the first 20 d of age. However, it was significantly higher for ad libitum milk fed calves at d 30, 40, 50, 60 (weaning) and 90 (post weaning) of their age(p<0.05). Similar body weights for both conventionally and ad libitum milk fed calves were observed for the post weaning period at 120, 150 and 180 d of age. Feed efficiency was significantly higher for conventionally fed calves than ad libitum milk fed calves(p<0.05). No sign of diarrhea, fever or cough was observed in calves fed either conventionally or ad libitum milk during the experimental period. In conclusion, calves fed more milk remained healthy and gained higher weight before weaning and maintained this advantage at least up to 90 d of their age.

Simultaneous Determination of Carbohydrates and Organic Acids in Various Cultured Dairy Foods by High-Performance Liquid Chromatography: A Preliminary Study (다양한 낙농 발효유제품에서 HPLC를 이용하여 탄수화물과 유기산의 동시 검출에 관한 연구)

  • Kim, Dong-Hyeon;Hwang, Dae-Geun;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Song, Kwang-Young;Yim, Jin-Hyuk;Kim, Young-Ji;Kang, Il-Byung;Lee, Soo-Kyung;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.4
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    • pp.263-269
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    • 2015
  • Various carbohydrates (lactose, glucose, and fructose), lactic acid, uric acid, and acetoin were separated on a ZORBAX Carbohydrate Analysis column using the Agilent 1200 HPLC ChemStation$^{TM}$, and were identified according to retention times with 325 Dual Wavelength UV-Vis Detector and Refractive Index Detector with 0.013 N $H_2SO_4$ at a flow rate of 0.8 mL/min. In addition, the lactase activity of four commercial probiotic lactic acid bacteria during 6-hour incubation was determined using a high-performance liquid chromatography (HPLC) method. Among the tested samples, Bifidobacterium animalis subsp. lactis showed the greatest lactase activity, followed by Lactobacillus rhamnosus and Lactobacillus casei, with Streptococcus salivarius subsp. thermophilus showing the lowest activity. Therefore, this HPLC technique shows potential for evaluating the fermentation processes of probiotic lactic acid bacteria and could simultaneously confirm the degree of ripening in various fermented dairy foods within only a half hour.

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Evaluation of Scab Resistance and Effect of Photosynthetic Rates on Fruit Characteristics among Elite Pear Seedlings (배 우량계통의 검은별무늬병 저항성 평가 및 광합성률이 과실 품질에 미치는 영향 구명)

  • Won, Kyung-Ho;Kang, Sam-Seok;Kim, Yoon-Kyeong;Sherzod, Rajametov;Lim, Kyeong-Ho;Lee, Han-Chan
    • Korean Journal of Environmental Agriculture
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    • v.32 no.2
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    • pp.117-122
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    • 2013
  • BACKGROUND: The scab, which is caused by Venturia nashicola, gives serious damages to pear trees. 'Niitaka' accounts for 82% of areas in pear cultivation. However 'Niitaka' is a scab susceptible cultivar. So, most of Korean farmers who growing pear trees have suffered by economic losses with the scab. In this research, we evaluated the scab resistance among elite pear seedlings to clarify genetics about the scab resistance. And we analyzed photosynthetic features with these seedlings to develop suitable cultivar which is advantageous for producing quality fruits during the growth and development of plants. METHODS AND RESULTS: We measured the rates of scab incidence among seedlings in a field experiment condition and an in-vitro test. An in-vitro test has been done with field experiment-based results. We made plant materials by grafting branches of each seedlings with 'Kongbae' rootstocks. And they had been grown for one month. Then, scab conidia suspension is sprayed to seedlings and sustained for 40 days under the controlled environment. As the results, 6 seedlings displayed lower incidence rates than other seedlings and 'Niitaka'. We also measured instant photosynthetic rates of each seedlings to determine the correlation between photosynthetic rates and fruit characteristics. However, it seemed that there is no correlation between them. CONCLUSION(S): Among the seedlings, 6 seedlings displayed the higher resistance to scab than other seedlings and 'Niitaka'. This characteristics is considered to be come from the gene expression of European pear. And we found that photosynthetic rate in trees rarely does not influence the fruit characteristics. It is considered to be affected by cultivar's own characteristics.

Hormonal Changes in Cultured Medium on In Vitro Culture of Bovine Oviduct Epithelial Cells (BOEC) Supplemented with UK-4 (소 난관 상피 세포의 체외 배양시 IL-4의 첨가에 의한 배양액내 호르몬 변화)

  • Choi, S.H.;Cho, S.R.;Han, M.H.;Kim, H.J.;Son, D.S.;Sang, B.D.;Park, C.K.
    • Journal of Embryo Transfer
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    • v.21 no.4
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    • pp.281-286
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    • 2006
  • This study was conducted to investigate the hormonal changes in cultured medium during in vitro culture of bovine oviduct epithelial cells (BOEC) supplemented with interleukin (IL)-4 of 0.001, 0.01, 0.1 or 1 ng/ml. BOEC were collected from the oviduct and washed 3 times with 1% antibiotic-mycotic-DMEM medium and cultured at $39^{\circ}C$, 5% $CO_2$, 95% air for 24$\sim$120 hrs. The cultured media were analyzed hormonal changes with hormonal analyzing kit (progesterone (P4), estradiol (E2) : Perkin Elmer, USA) and Transforming growth factor (TGF)-$\beta$ with Eliza kit (Promega, USA). The production of P4 in 0.001 IL-4 was increased as the culture time increased. P4 production was significantly higher in the medium cultured for 120 hrs than 24 hrs (P<0.05). P4 production in 0.01 ng/ml group was similar to that of 0.001 ng/ml. The production of E2 in 0.001 and 0.01 ng/ml groups were increased to 72 hrs like P4 production and showed significantly different between the culture periods (P<0.05). After the culture for 96 hrs, P4 and E2 production were increased to 96 hrs, but decreased at 120 hrs. The production of TGF-$\beta$ showed no changes according to culture period or supplementation of IL-4. In conclusion, the supplementation of IL-4 can increase the production of P4 and E2 and might have important role for the successful pregnancy in bovine.