• Title/Summary/Keyword: M/G/c/K

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Characterization of a Restriction Endonuclease, SdiI from Streptomyces diastatochromogenes (Streptomyces diastatochromogenes로부터 분리된 SdiI의 특성에 관한 연구)

  • Bae, Moo;Song, Eun-Sook;Hwang, Hye-Yeon;Yim, Jeong-Bin
    • Korean Journal of Microbiology
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    • v.32 no.4
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    • pp.301-305
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    • 1994
  • In catalytic properties of the restriction enonuclease, SdiI, which was purified from Streptomyces diastatochromogenes, this enzyme was active at wide range between pH 7.0 and 12.5, and up to $60^{\circ}C$ and 500 mM of NaCl concentration. It was stable between 20^{\circ}C$ and $60^{\circ}C$, and essentially requires $MgCl_2$ for endonuclease activity. The restriction map of lambda DNA which was obtained by double digestion with various enzymes suggested SdiI to be an isoschizomer of XhoI. From the determination of restriction site based on DNA sequencing method, recognition and cleavage specificity of SdiI was concluded as: 5‘-C${\downarrow}$TCGA G-3' 3'-G AGCT${\uparrow}$C-5'

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Effect of Relative Humidity and Storage Temperature on the Quality of Green Tea Powder (상대습도 및 저장 온도가 분말녹차의 품질에 미치는 영향)

  • Lee, Jung-Min;Lim, Sang-Wook;Cho, Sung-Hwan;Choi, Sung-Gil;Heo, Ho-Jin;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.1
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    • pp.83-88
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    • 2009
  • After storing green tea powder for three months at three different temperatures (-20, 4, and $20^{\circ}C$) with three different relative humidities (RHs) (23, 69, and 81%), the chemical quality was evaluated with green tea, which was prepared by soaking 1.5 g of the powder into 100 mL of distilled water at $70^{\circ}C$ for 5 min. Total phenolic contents, total flavanol contents, and ascorbic acid contents of green tea powder stored at $4^{\circ}C$ with 23% RH changed from 267.5, 49.4, and 24.2 mg/g to 287.1, 44.9, and 36.9 mg/g, respectively, compared to the powder before storage. EGC and EGCG, the main catechins of green tea, also changed from 16.9 and 27.3 mg/g to 24.3 and 36.5, g/g, respectively, after storage for 3 months at $4^{\circ}C$ with 23% RH. However, when the green tea powder was stored at -20 or $20^{\cric}C$ with higher RH such as 69 and 81%, the chemical compounds were significantly decreased. The results indicate that temperature and RH are important during storage of green tea powder, and low RH and refrigerated condition ($-4^{\cric}C$) are preferable to increase or preserve the chemical compounds of the tea.

In vitro anticancer and antioxidant effects of acetone extract of Eucommia ulmoides oliver leaves (두충잎 아세톤 추출물의 in vitro 항암 및 항산화 효과)

  • In, Man-Jin;Kim, Eun Jeong;Kim, Dong Chung
    • Journal of Applied Biological Chemistry
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    • v.61 no.2
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    • pp.119-124
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    • 2018
  • In vitro anticancer and antioxidant effects of acetone extract from leaves of Eucommia ulmoides Oliver were investigated. The extraction yield and total phenolic content of the acetone extract were $1.13{\pm}0.033%$ (w/w) and $36.7{\pm}1.96mg$ gallic acid equivalents/g-extract, respectively. $GI_{50}$ values of the acetone extract for human non-small cell lung cancer cells (A549), human colon cancer cells (SNU-C4), human cervical cancer cells (HeLa), and human embryonic lung epithelial cell (L132) were 53.4, 53.8, 88.3, and $153.9{\mu}g/mL$, respectively. The acetone extract effectively inhibited the proliferation of human non-small cell lung cancer (A549) and colon cancer (SNU-C4) cells in a concentration-dependent manner, but was less cytotoxic with human normal cells (L132). $EC_{50}$ values of the acetone extract for free radical scavenging, reducing power, and lipid peroxidation inhibition were about 2,000, 275.8, and $257.9{\mu}g/mL$, respectively. The acetone extract showed a potent reducing power and lipid peroxidation inhibitory activity in a concentration-dependent manner.

Synergistic Effect of Physico-chemical Treatment and Bacteriocin Produced by Enterococcus faecium MJ-14 (Enterococcus faecium MJ-14가 생산하는 박테리오신과 물리화학적 처리의 상승효과)

  • Lim Sung-Mee
    • Journal of Food Hygiene and Safety
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    • v.20 no.4
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    • pp.217-224
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    • 2005
  • When L. monocytogenes ($10^{5}CFU/mL$) at exponential phase cells were heated for 5 min at $65^{\circ}C$ in the presence of the bacteriocin (30 BU/mL) produced by E. faecium MJ-14, the number of viable cells was markedly reduced at p < 0.05. The bactericidal effect of bacteriocin showed synergism with combination ot organic acids (citric acid or acetic acid) or chemical preservatives (sodium benzoate, sodium lactate, sodium nitrate or potassium nitrate). For example, the number of viable cells was reduced by 4.8 log units under combination of the bacteriocin (30 BU/mL) and sodium nitrate ($100{\mu}g/mL$), while it was reduced by 1.1 log unit only under single treatment of the bacteriocin after 12 k at $37^{\circ}C$. The addition of the bacteriocin (300 BU/mL) into skim milk inoculated with L. monocytogenes ($10^{5}$CFU/mL) reduced the cells by 1.5 log unit, in case of the cell suspension stored at $4^{\circ}C$ for 24 hr. Moreover, L. monocytogenes was reduced by 2 log unit when stored at $-20^{\circ}C$ for 7 days in gound pork added with 300 BU/mL of 린e bacteriocin.

A Study on the Sensory Characteristics of Korean Red Wine (한국산 적포도주의 관능적 특성에 관한 연구 (III))

  • Lee, Jang-Eun;Hong, Hee-Do;Choi, Hee-Don;Shin, Yong-Sub;Won, Yoo-Dong;Kim, Sung-Soo;Koh, Kyung-Hee
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.841-848
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    • 2003
  • The sensory characteristics of red wine Gerbong (G), Campbell (C), Moru (M), Gerbong + Moru (70 : 30, GM), Gerbong + Campbell (70 : 30, GC) and French wine (F, Carbernet Sauvignon, 1998) were evaluated. The preferences of color, flavor, taste and total evaluation were determined by a ranking test, and the organoleptic characteristics were evaluated by a quantitative descriptive analysis (QDA) method. The mean color scores of C, GM, F, GC, M and G were 4.74, 3.94, 4.67, 3.70, 2.65 and 1.47, respectively (p<0.001). The order for the mean score for flavor was GM (4.12) = M (3.94) = C (3.76) = F (3.76) ${\geq}$ GC (3.12)>G (2.29) (p<0.01), and the order for taste was F (4.75) ${\geq}$ C (4.25) ${\geq}$ GM (3.37) = GC (3.50) ${\geq}$ G (2.75) = M (2.37) (p<0.001). The total evaluation of mean scores showed G, M, C, GM, GC and F were 237, 2.44, 4.06, 3.87, 3.64 and 4.81, respectively (p<0.001). Influences of sensory characteristics on the total evaluation, in percentages, were 69.3% for taste, 3.7% for color, and 1.5% for flavor. The influences of taste, color, and flavor in red wine were 17% for sweet, acid, bitter and salty taste, 28.9% for purple and red color, and 14.4% for grape flavor. The attributes of the purple and red colors showed a positive correlation with grape flavor, oak flavor, grape taste, and floral tastes, but a negative correlation with $SO_2$, flavor. The attribute of sweet taste showed a positive correlation with grape flavorand floral flavor, but a negative correlation with bitter and astringency tastes, according to Pearsons correlation analysis (p<0.01).

Anti-proliferating Effects of Porphyra tenera Fractions on Several Cancer Cell Lines in uitro (김 분획물의 in vitro에서의 항발암효과)

  • Shin, Mi-Ok;Bae, Song-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.10
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    • pp.1514-1519
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    • 2005
  • This study was performed to investigate the effects of Porphyra tenera (PT) on cytotoxicity and quinone reductase (QR) activity in the cancer cells. PT was extracted with methanol and further fractionated into five different types: hexane (PTMH), ethyl-ether (PTMEE), ethylacetate (PTMEA) butanol (PTMB) and aquous (PTMA) partition layers. We determined the cytotoxic effect of these layers on C6, HepG2, MCF-7, and HT-29 cell lines by MTT assay. Among the various fractions, hexane (PTMH) of PT showed the strongest cytotoxic effect on C6, HepG2 and MCF-7 cell lines. PTMH displayed very low level of cytotoxicity at the lower concentration levels and at 300 $\mu$g/mL. PTMH resulted in 87.5$\%$ growth inhibition on C6 cell 70 $\%$ on the HepG2 cell and 89$\%$ on the MCF-7 cell, which were significantly high compared to other fractions. A 400 $\mu$g/mL PTMH concentration level, 99$\%$, 94.5$\%$ and 99$\%$ of cell growth inhibition were resulted on the same cell lines. On HT-29 cell line, both hexane (PTMH) and aqueous (PTMA) fraction of PT showed cytotoxic effects, but the Percentage was not as high as previous results tested on other cell lines such as C6 HepG2 and MCF-7 cell lines. Also, we observed quinone reductase (QR) inducing-effects in all fractions of PT on HepG2 cells. The QR inducing effects of the PTMH on HepG2 cells at 150 $\mu$g/mL concentration was 6.6 times higher than the control. Although further studies are needed, the present work suggests that PT was a potential to be used as a chemopreventive.

The Magnetic Relaxation Properties of DTPA-bis(4-carboxycyclohexyl) amide Paramagnetic Gd-chelates (DTPA-bis(4-carboxycyclohexyl)amide 상자성 복합체의 자기이완특성에 관한 연구)

  • Kim, In-Sung;Lee, Young-Ju;Lee, Jae-Jun;Kim, Ju-Hyun;Kim, Yoo-Kyung;Sujit, Dutta;Kim, Suk-Kyung;Kim, Tae-Jeong;Kang, Duk-Sik;Chang, Yong-Min
    • Investigative Magnetic Resonance Imaging
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    • v.10 no.1
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    • pp.20-25
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    • 2006
  • Purpose : To evaluate the NMR relaxation properties of newly developed high performance paramagnetic complexes. Materials and methods : 4-aminomethylcyclohexane carboxylic acid (0.63g, 4 mmol) was mixed with the suspension solution of DMF (15mL) and DTPA-bis-anhydride (0.71g, 2 mmol) to synthesize the ligand. The ligand was then mixed with Gd2O3 (0.18g, 0.5 mmol) to synthesize Gd-chelate. For the measurement of magnetic relaxivity of paramagnetic compounds, the compounds were diluted to 1mM and then the relaxation times were measured at 1.5T(64 MHz). Inversion-recovery pulse sequence was employed for T1 relaxation measurement and CPMG(Carr-Purcell-Meiboon-Gill) pulse sequence was employed for T2 relaxation measurement. Using MATLAB(Version 7.1) program, T1 magnetic relaxation map, R1 map, T2 magnetic relaxation map and R2 map were developed to represent magnetic relaxation time and magnetic relaxivity as image. Results : Compared to $R1=4.9mM^{-1}sec^{-1}$ and $R2=4.8mM^{-1}sec^{-1}$ of Omniscan (Gadodiamide), which is commercially available paramagnetic MR agent, R1 of SUK090(Gd-C32H74N5O24) was $12.46mM^{-1}sec^{-1}$ and R1 of SUK091(Gd-C34H78N5O24) was $12.77mM^{-1}sec^{-1}$. However, R1 of SUK092(Gd-C30H56N5O17) was decreased to $2.09mM^{-1}sec^{-1}$. In case of R2, SUK090(Gd-C32H74N5O24) was $8.76mM^{-1}sec^{-1}$ and SUK091(Gd-C34H78N5O24) was $7.60mM^{-}1sec^{-1}$ whereas SUK092(Gd-C30H56N5O17) was decreased to $1.82mM^{-1}sec^{-1}$. Conclusion : Among three new paramagnetic complexes, SUK090(Gd-C32H74N5O24) and SUK091(Gd-C34H78N5O24) showed higher T1, T2 magnetic relaxation rates than that of commercially available paramagnetic MR agent and thus expected to have more contrast enhancement effect.

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Physiological Characteristics and GABA Production of Lactobacillus plantarum K74 isolated from Kimchi (김치에서 분리한 Lactobacillus plantarum K74의 GABA 생산 및 생리적 특성)

  • Park, Sun-Young;Shim, Hye-Young;Kim, Kee-Sung;Lim, Sang-Dong
    • Journal of Dairy Science and Biotechnology
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    • v.31 no.2
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    • pp.143-152
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    • 2013
  • Aminobutyric acid (GABA), a major inhibitory neurotransmitter in the central nervous system of animals, has several physiological effects including anti-hypertensive, diuretic, tranquilizing, and anti-stress properties, in humans. The purpose of this study was to investigate Lactobacillus plantarum K74, which was isolated from kimchi and selected as a strain with a high ability to produce GABA, to develop a new starter culture for fermented milk production. L. plantarum K74 produced $134.52{\mu}g/mL$ GABA in MRS broth containing 1% MSG, $212.27{\mu}g/mL$ GABA in MRS broth containing 2% MSG, and $234.63{\mu}g/mL$ GABA in MRS broth containing 3% MSG. The optimum growth temperature of L. plantarum K74 was $34^{\circ}C$, reaching a pH of 4.4 after 18 hours of growth. L. plantarum K74 was most sensitive to novobiocin out of 16 different antibiotics tested, and was most resistant to kanamycin and polymyxin B. L. plantarum K74 did not produce ${\beta}$-glucuronidase, a carcinogenic enzyme, and was comparatively tolerant to bile juice and low pH. Furthermore, it displayed resistance to Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus at rates of 54.9%, 46.3%, and 0.7%, respectively.

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Star Cluster System in M33

  • Park W. -K.;Lee M. G;Park H. S.;Kim S. C.;Park J. -H.;Lee S. -G.;Oh S. J;Lee Y -W.;Sohn Y. -J.;Rey S. -C.;Yuk I. -S.;Kim H. -I.;Han W.
    • The Bulletin of The Korean Astronomical Society
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    • v.29 no.2
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    • pp.26.2-26.2
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    • 2004
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