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Synergistic Effect of Physico-chemical Treatment and Bacteriocin Produced by Enterococcus faecium MJ-14  

Lim Sung-Mee (Department of Hotel Cuilinary Arts, Tongmyong College)
Publication Information
Journal of Food Hygiene and Safety / v.20, no.4, 2005 , pp. 217-224 More about this Journal
Abstract
When L. monocytogenes ($10^{5}CFU/mL$) at exponential phase cells were heated for 5 min at $65^{\circ}C$ in the presence of the bacteriocin (30 BU/mL) produced by E. faecium MJ-14, the number of viable cells was markedly reduced at p < 0.05. The bactericidal effect of bacteriocin showed synergism with combination ot organic acids (citric acid or acetic acid) or chemical preservatives (sodium benzoate, sodium lactate, sodium nitrate or potassium nitrate). For example, the number of viable cells was reduced by 4.8 log units under combination of the bacteriocin (30 BU/mL) and sodium nitrate ($100{\mu}g/mL$), while it was reduced by 1.1 log unit only under single treatment of the bacteriocin after 12 k at $37^{\circ}C$. The addition of the bacteriocin (300 BU/mL) into skim milk inoculated with L. monocytogenes ($10^{5}$CFU/mL) reduced the cells by 1.5 log unit, in case of the cell suspension stored at $4^{\circ}C$ for 24 hr. Moreover, L. monocytogenes was reduced by 2 log unit when stored at $-20^{\circ}C$ for 7 days in gound pork added with 300 BU/mL of 린e bacteriocin.
Keywords
Enterococcus faecium MJ-14; Listeria monocytogenes; bacteriocin, synergistic effect;
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1 Mundt, J. O.: Occurrence of enterococci on plants in a wild environment. Appl. Microbiol., 11, 141-144 (1963)
2 Giraffa, G., Picchioni, N., Neviani, E. and Carminati, D.: Production and stability of an Enterococcus faecium bacteriocin during Taleggio cheesemaking and ripening. Food Microbiol., 12, 301-307 (1995)   DOI   ScienceOn
3 Franz C. M., Schillinger, U. and Holzapfel, W. H.: Produced and characterization of enterocin 900, a bacteriocin produced by Enterococcus faecium BFE 900 from black olives. Int. J. Food Microbiol., 29, 255-270 (1996)   DOI   PUBMED   ScienceOn
4 Holzapfel, W. H., Haberer, P., Snel, J. and Schillinger, U.: Overview of gut flora and probiotics. Int. J. Food. Microbiol., 41, 85-101 (1998)   DOI   ScienceOn
5 Muriana, P. M.: Bacteriocins for control of Listeria spp. in food. J. Food Prot., Supplement, 54-63 (1996)
6 Torri, T. G., Carminati, D. and Giraffa, G.: Production of bacteriocins active against Listeria monocytogenes and Listeria innouca from dairy enterococci. Food Microbiol., 11, 243-252 (1994)   DOI   ScienceOn
7 Ennahar, S. and Deschamps, N.: Anti-Listeria effect of enterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins from lactic acid bacteria. J. Appl. Microbiol., 88, 449-457 (2000)   DOI   ScienceOn
8 김영목, 박욱연, 목종수, 장동석: Listeria monocytogenes YM-7의 생리적 특성. 한국수산학회지, 28, 443-450 (1995)
9 Garcia, M. T., Canamero, M. M., Lucas, R., Omar, N. B., Pulido, R. P. and Galez, A.: Inhibition of Listeria monocytogenes by enterocin EJ 97 produced by Enterococcus faecalis EJ97. Int. J. Food Microbiol., 90, 161-170 (2004)   DOI   ScienceOn
10 Nielsen, J. W., Dickson, J. S. and Crouse, J. D.: Use of a bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl. Environ. Microbiol., 56, 2142-2145 (1990)
11 Abriouel, H., Valdivia, E., Galvez, A. and Maqueda, M.: Response of Salmonella choleraesuis LT2 spheroplasts and permeabilized cells to the bacteriocin AS-48. Appl. Environ. Microbiol., 64, 4623-4626 (1998)
12 Lim, S. M., Park, M. Y. and Chang, D. S.: Characterization of bacteriocin produced by Enterococcus faecium MJ-14 isolated from Meju. Food Sci. Biotechnol., 14, 49-57 (2005)
13 Yousef, A. E., Luchansky, J. B., Deganan, A. J. and Doyle, M. P.: Behavior of L. monocytogenes in wiener exudates in the presence of Pediococcus acidilactici H on pediocin AcH during storage at 4 or $25^{\circ}C$. Appl. Environ. Microbiol., 57, 1461-1467 (1991)
14 Degnan, A. J., Buyong, N. and Luchansky, J. B.: Antilisterial activity of pediocin AcH in model food systems in the presence of an emulsifier or encapsulated within liposomes. Int. J. Food Microbiol., 18, 127-138 (1993)   DOI   ScienceOn
15 Kim, S. Y., Lee, Y. M., Lee, S. Y., Lee, Y. S., Kim, J. H., Ahn, C., Kang, B. C. and Ji, G. E.: Synergistic effect of citric acid and pediocin K1, a bacteriocin produced by Pediococcus sp. K1, on inhibition of Listeria monocytogenes. J. Microbiol. Biotechnol., 11, 831-837 (2001)
16 Wan, J., Harmark, K., Davidson, B. E., Hillier, A. J., Gordon, J. B., Wilcock, A., Hickey, M. W. and Coventry, M. J.: Inhibitory of Listeria monocytogenes by piscicolin 126 in milk and Camembert cheese manufactured with a thermophilic starter. J. Appl. Microbiol., 82, 273-280 (1997)   DOI   ScienceOn
17 Charles, M. A. P. F., Holzapfel, W. H. and Stiles, M. E.: Enterococci at the crossroads of food safety? Int. J. Food Microbiol., 47, 1-24 (1999)   DOI   PUBMED   ScienceOn
18 정동선, 이영경: 박테리오신 생성 젖산균을 이용한 항충치 활성을 지닌 발효이온음료 개발. 한국식품영양과학회지, 31, 399-404 (2002)
19 Monica, B. W., Farias, M. E., Takeda, E., Sesma, F., Holgado, A. P., Torres, R. A. and Coto, C. E.: Antiviral activity of enterocin CRL35 against herpesviruses. Int. J. Antimicro. Agents, 12, 293-299 (1999)   DOI   ScienceOn
20 Laukova, A. and Czikkova, S.: The use of enterocin CCM 4231 in soy milk to control the growth of Listeria moncytogenes and Staphylococcus aureus. J. Appl. Microbiol., 87, 182-186 (1999)   DOI   ScienceOn
21 Park, H. J., Lee, N. K., Choi, J. O., Ha, J. U. and Paik, H. D.: Control of Listeria monocytogenes in ground beef by Lactococcal bacteriocins. Food Sci. Biotechnol., 10, 199-203 (2001)
22 Aymerich, T., Artigas, M. G., Garriga, M., Monfort, J. M. and Hugas, M.: Effect of sausage ingredients and additives on the production of enterocins A and B by Enterococcus faecium CTC492. Optimization of in vitro production and anti-listerial effect in dry fermented sausages. J. Appl. Microbiol., 88, 686- 694 (2000)   DOI   ScienceOn
23 Nykanen, A., Vesanen, S. and Kallio, H.: Synergistic antimicrobial effect of nisin whey permeated and lactic acid on microbes isolated from fish. Lett. Appl. Microbiol., 27, 345- 348 (1998)   DOI   ScienceOn
24 Simonetta, A. C., Velasco, L. G. M. and Frison, L. N.: Antibacterial activity of enterococci strains against Vibrio cholerae. Lett. Appl. Microbiol., 24, 139-143 (1997)   DOI   ScienceOn
25 Sabia, C., Niederhausern, S., Messi, P., Manicardi, G. and Bondi, M.: Bacteriocin-producing Enterococcus casseliflavus IM 416K1, a natural antagonist for control of Listeria monocytogenes in Italian sausages ('cacciatore'). Int. J. Food Microbiol., 87, 173-179 (2003)   DOI   PUBMED   ScienceOn
26 Michael, G. G., Weber, S. and Hammes, W. P.: Effect of ecological factors on the inhibitory spectrum and activity of bacteriocin. Int. J. Food Microbiol., 46, 207-217 (1999)   DOI   ScienceOn
27 Parente, E. and Hill, C.: Inhibition of Listeria in buffer, broth and milk by enterocin 1146, a bacteriocin produced by Enterococcus faecium. J. Food Prot., 55, 503-508 (1992)   DOI
28 한경식, 오세종, 문용일, 김세헌: 복합 박테리오신의 항균활성 및 축산식품 저장성 증진 효과. 한국축산식품학회지, 22, 164-171 (2002)
29 Schillinger, U., Kaya, M. and Lucke, F. K.: Behaviour of Listeria monocytogenes in meat and its control by a bacteriocin-producing strain of Lactobacillus sake. J. Appl. Bacteriol., 70, 473-478 (1991)   DOI
30 Lewenstein, A., Frigerio, G. and Moroni, M.: Biological properties of SF68, a new approach for the treatment of diarrhoeal diseases. Curr. Ther. Res., 26, 967-981 (1979)
31 Moreno, M. R. F., Rea, M. C., Cogan, T. M. and Vuyst, L.: Applicability of a bacteriocin-producing Enterococcus faecium as a co-culture in Cheddar cheese manufacture. Int. J. Food Microbiol., 81, 73-84 (2002)   DOI   ScienceOn
32 이정옥, 류충호: Nisin 생성 유산균을 이용한 저염 된장의 제조. 한국식품영양과학회지, 31, 75-80 (2002)
33 Moreno, M. R. F., Callewaert, R., Devreese, B., Beeumen, J. V. and Vuyst, L.: Isolation and biochemical characterization of enterocins produced by enterococci from different sources. J. Appl. Microbiol., 94, 214-229 (2003)   DOI   ScienceOn