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Antimicrobial resistance studies in staphylococci and streptococci isolated from cows with mastitis in Argentina

  • Elisa, Crespi;Ana M., Pereyra;Tomas, Puigdevall;Maria V., Rumi;María F., Testorelli;Nicolas, Caggiano;Lucia, Gulone;Marta, Mollerach;Elida R., Gentilini;Mariela E., Srednik
    • Journal of Veterinary Science
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    • v.23 no.6
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    • pp.12.01-12.10
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    • 2022
  • Background: Staphylococcus aureus and Streptococcus agalactiae are the main cause of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative staphylococci (CNS) and environmental streptococci are the main cause of subclinical mastitis. Bacteria isolated from infected animals show increasing antimicrobial resistance. Objectives: This study aims to determine the antimicrobial resistance of staphylococci and streptococci isolated from milk with mastitis, and to genotypically characterize the methicillin-resistant (MR) staphylococci. Methods: Isolation was performed on blood agar and identification was based on biochemical reactions. Antimicrobial susceptibility was according to the Clinical and Laboratory Standards Institute guidelines. The antimicrobial resistance genes, SCCmec type and spa type were detected by the polymerase chain reaction method. Results: We isolated a total of 185 staphylococci and 28 streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to clindamycin. Conclusions: The present findings of this study indicate a development of antimicrobial resistance in main bacteria isolated from cows with mastitis in Argentina.

Profile and Outcome of Management of Brain Tumours in Kaduna Northwestern Nigeria

  • Danjuma, Sale;Dauda, Happy Amos;Kene, Aghadi Ifeanyi;Akau, Kache Stephen;Jinjiri, Ismail Nasiru
    • Journal of Korean Neurosurgical Society
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    • v.65 no.5
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    • pp.751-757
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    • 2022
  • Objective : Tumours of the brain are a rare occurrence accounting for approximately 2% of all neoplasms in adults. Few studies have been done in Nigeria on the profile of brain tumours. The aim of this study is to determine the profile of brain tumours in general and determine the change in Kanofsky Performance Score (KPS) after treatment. Methods : This is a prospective hospital-based study in Kaduna. All consecutive patients over 18 years of age with diagnosis of brain tumours from January 2016 to December 2019 were included in the study. Demographic and clinical data was collected using a proforma during the study. Patients who received treatment were followed up for 12 months. The primary outcome data was the difference in the quality of life as measured by KPS at the point of first contact and at 1-month after treatment and at 12-month follow up. Data obtained was analysed with SPSS version 25.0 for Windows. Descriptive statistics was done to determine the profile. Paired t-test at 95% confidence interval was done to check for significant correlation between the mean KPS. Results : A total of 39 consecutive patients were included in the study. There was a slight male preponderance with a M : F of 1.17 : 1. Meningioma and metastasis were more common in females while gliomas and pituitary tumours were more common in males. The mean age of patients was 49.8 years and standard deviation of 11.8 years. Pituitary tumours were the most common tumours. The most common location of the tumour was frontal lobe followed by the pituitary gland. The mean duration of symptoms before neurosurgical consultation was 38 weeks. The most common presenting symptoms of patient with brain tumour was headache. The quality of life improve compare to the baseline in 81% of patient at discharge and at 1 year follow up. The overall mortality rate was 25.6%. Conclusion : The most common brain tumour in our study is pituitary tumour. Most patients present late. The most common presenting symptoms is headache. There is significant improvement in the KPS of patients following treatment. The overall mortality rate at 1-year post treatment is 25.6%.

A basic study for explosion pressure prediction of hydrogen fuel vehicle hydrogen tanks in underground parking lot (지하주차장 수소연료차 수소탱크 폭발 압력 예측을 위한 기초 연구)

  • Lee, Ho-Hyung;Kim, Hyo-Gyu;Yoo, Ji-Oh;Lee, Hu-Yeong;Kwon, Oh-Seung
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.23 no.6
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    • pp.605-612
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    • 2021
  • Amid growing global damage due to abnormal weather caused by global warming, the introduction of eco-friendly cars is accelerating to reduce greenhouse gas emissions from internal combustion engines. Accordingly, many studies are being conducted in each country to prepare for the explosion of hydrogen fuel in semi-closed spaces such as tunnels and underground parking lots to ensure the safety of hydrogen-electric vehicles. As a result of predicting the explosion pressure of the hydrogen tank using the equivalent TNT model, it was found to be about 1.12 times and 2.30 times higher at a height of 1.5 meters, respectively, based on the case of 52 liters of hydrogen capacity. A review of the impact on the human body and buildings by converting the predicted maximum explosive pressure into the amount of impact predicted that all predicted values would result in lung damage or severe partial destruction. The predicted degree of damage was applied only by converting the amount of impact caused by the explosion, and considering the additional damage caused by the explosion, it is believed that the actual damage will increase further and safety and disaster prevention measures should be taken.

Analysis of sedation and general anesthesia in patients with special needs in dentistry using the Korean healthcare big data

  • Kim, Jieun;Kim, Hyuk;Seo, Kwang-Suk;Kim, Hyun Jeong
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.22 no.3
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    • pp.205-216
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    • 2022
  • Background: People with special needs tend to require diverse behavioral management in dentistry. They may feel anxious or uncomfortable or may not respond to any communication with the dentists. Patients with medical, physical, or psychological disorders may not cooperate and therefore require sedation (SED) or general anesthesia (GA) to receive dental treatment. Using the healthcare big data in Korea, this study aimed to analyze the trends of SED and GA in special needs patients undergoing dental treatment. It is believed that these data can be used as reference material for hospitals and for preparation of guidelines and related policy decisions of associations or governments for special needs patients in dentistry. Methods: The study used selected health information data provided by the Korean National Health Insurance Service. Patients with a record of use of one of the eight selected drugs used in dental SED between January 2007 and September 2019, those with International Classification of Diseases-10 codes for attention deficit hyperactivity disorder (ADHD), phobia, brain disease, cerebral palsy, epilepsy, genetic disease, autism, mental disorder, mental retardation, and dementia were selected. The insurance claims data were analyzed for age, sex, sedative use, GA, year, and institution. Results: The number of special needs patients who received dental treatment under SED or GA from January 2007 to September 2019 was 116,623. Number of SED cases was 136,018, performed on 69,265 patients, and the number of GA cases was 56,308, implemented on 47,257 patients. In 2007, 3100 special needs patients received dental treatment under SED while in 2018 the number of cases increased 6 times to 18,528 SED cases. In dentistry, ADHD was the most common disability for SED cases while phobia was the most common cause of disability for GA. The male-to-female ratio with respect to SED cases was higher for males (M : F = 64.36% : 35.64%). Conclusion: The application of the SED method and GA for patients with special needs in dentistry is increasing rapidly; thus, preparing guidelines and reinforcing the education and system are necessary.

Effect of Culture Media on Production of Biomass, Fatty Acid, and Carotenoid in a Newly Isolated Mychonastes sp. (신규 분리된 Mychonastes sp.의 생장, 지방산 및 색소 생산에 생장배지가 미치는 영향)

  • Yim, Kyung June;Jang, Hyun-Jin;Park, Yeji;Nam, Seung Won;Hwang, Byung Su;Jung, Ji Young;Lee, Chang Soo;Kim, Z-Hun
    • Journal of Marine Bioscience and Biotechnology
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    • v.14 no.1
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    • pp.1-8
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    • 2022
  • This study examined the growth, fatty acid (FA) content, and carotenoids of a newly isolated freshwater microalga, Mychonastes sp. 246, in various culture media. The appropriate temperature and light intensity for culturing Mychonastes sp. 246 were determined as 18℃-22℃ and 200-250 µmol/m2/s using a high throughput photobioreactor. The microalgal cells were cultivated in 0.5 L bubble column photobioreactors using BG11, Bold's Basal media, and f/2 media. According to the growth results of the microalgae, BG11, among the tested media, showed the highest biomass concentrations (3.5 ± 0.1 g/L in 10 d). To enhance the biomass growth of the microalgae, the N:P ratio in BG11 was manipulated from 45:1 to 7:1 based on the stoichiometric cell composition. The biomass concentrations of Mychonastes sp. 246 grown on the manipulated BG11 (MBG) increased to 38% (4.6 ± 0.3 g/L in d) compared with the original BG11 (3.3 g/L). The FA content of the microalgae grown on the MBG was lower (8.4%) than that of the original BG11 (10.1%) while the FA compositions did not exhibit any significant differences. Furthermore, three kinds of carotenoids were identified in Mychonastes sp. 246, zeaxanthin, lutein, and β-carotene. These results suggest an effective strategy for increasing biomass concentrations, FA content, and carotenoids of microalgae by performing a simple N:P adjustment in the culture media.

NIRS Analysis of Liquid and Dry Ewe Milk

  • Nunez-Sanchez, Nieves;Varo, Garrido;Serradilla-Manrique, Juan M.;Ares-Cea, Jose L.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1251-1251
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    • 2001
  • The routine analysis of milk chemical components is of major importance both for the management of animals in dairy farms and for quality control in dairy industries. NIRS technology is an analytical technique which greatly simplifies this routine. One of the most critical aspects in NIRS analysis of milk is sample preparation and analysis modes which should be fast and straightforward. An important difficulty when obtaining NIR spectra of milk is the high water content (80 to 90%) of this product, since water absorbs most of the infrared radiation, and, therefore, limits the accuracy of calibrating for other constituents. To avoid this problem, the DESIR system was set up. Other ways of radiation-sample interaction adapted for liquids or semi-liquids exist, which are practically instantaneous and with limited or null necessity of sample preparation: Transmission and Folded Transmission or Transflectance. The objective of the present work is to compare the precision and accuracy of milk calibration equations in two analysis modes: Reflectance (dry milk) and Folded Transmission (liquid milk). A FOSS-NIR Systems 6500 I spectrophotometer (400-2500 nm) provided with a spinning module was used. Two NIR spectroscopic methods for milk analysis were compared: a) folded transmission: liquid milk samples in a 0.1 pathlength sample cell (ref. IH-0345) and b) reflectance: dried milk samples in glass fibre filters placed in a standard ring cell. A set of 101 milk samples was used to develop the calibration equations, for the two NIR analysis modes, to predict casein, protein, fat and dry matter contents, and 48 milk samples to predict Somatic Cell Count (SCC). The calibrations obtained for protein, fat and dry matter have an excellent quantitative prediction power, since they present $r^2$ values higher than 0.9. The $r^2$ values are slightly lower for casein and SCC (0.88 and 0.89 respectively), but they still are sufficiently high. The accuracy of casein, protein and SCC equations is not affected by the analysis modes, since their ETVC values are very similar in reflectance and folded transmission (0.19% vs 0.21%; 0.16% vs 0.19% and 55.57% vs 53.11% respectively), Lower SECV values were obtained for the prediction of fat and dry matter with the folded transmission equations (0.14% and 0.25% respectively) compared to the results with the reflectance ones (0.43% and 0.34% respectively). In terms of accuracy and speed of analytical response, NIRS analysis of liquid milk is recommended (folded transmission), since the drying procedure takes 24 hours. However, both analysis modes offer satisfactory results.

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Evaluation of Human Demonstration Augmented Deep Reinforcement Learning Policy Optimization Methods Using Object Manipulation with an Anthropomorphic Robot Hand (휴먼형 로봇 손의 사물 조작 수행을 이용한 인간 행동 복제 강화학습 정책 최적화 방법 성능 평가)

  • Park, Na Hyeon;Oh, Ji Heon;Ryu, Ga Hyun;Anazco, Edwin Valarezo;Lopez, Patricio Rivera;Won, Da Seul;Jeong, Jin Gyun;Chang, Yun Jung;Kim, Tae-Seong
    • Proceedings of the Korea Information Processing Society Conference
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    • 2020.11a
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    • pp.858-861
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    • 2020
  • 로봇이 사람과 같이 다양하고 복잡한 사물 조작을 하기 위해서 휴먼형 로봇손의 사물 파지 작업이 필수적이다. 자유도 (Degree of Freedom, DoF)가 높은 휴먼형(anthropomorphic) 로봇손을 학습시키기 위하여 사람 데모(human demonstration)가 결합된 강화학습 최적화 방법이 제안되었다. 본 연구에서는 강화학습 최적화 방법에 사람 데모가 결합된 Demonstration Augmented Natural Policy Gradient(DA-NPG)와 NPG 의 성능 비교를 통하여 행동 복제의 효율성을 확인하고, DA-NPG, DA-Trust Region Policy Optimization (DA-TRPO), DA-Proximal Policy Optimization (DA-PPO)의 최적화 방법의 성능 평가를 위하여 6 종의 물체에 대한 휴먼형 로봇손의 사물 조작 작업을 수행한다. 그 결과, DA-NPG 와 NPG를 비교한 결과를 통해 휴먼형 로봇손의 사물 조작 강화학습에 행동 복제가 효율적임을 증명하였다. 또한, DA-NPG 는 DA-TRPO 와 유사한 성능을 보이면서 모든 물체에 대한 사물 파지에 성공하여 가장 안정적이었다. 반면, DA-TRPO 와 DA-PPO 는 사물 조작에 실패한 물체가 존재하여 불안정한 성능을 보였다. 본 연구에서 제안하는 방법은 향후 실제 휴먼형 로봇에 적용하여 휴먼형 로봇 손의 사물조작 지능 개발에 유용할 것으로 전망된다.

A Study on the Vegetables Mentioned in the Bible (성서에 언급된 채소류에 관한 연구)

  • Woo, Ja-Won
    • Journal of the Korean Society of Food Culture
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    • v.22 no.1
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    • pp.1-9
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    • 2007
  • This paper is intended to study what kinds of the vegetables are mentioned in the Bible and how they were used in those days. While one hundred and twenty-eight different plants are mentioned in the Bible, there are today 2,384 plant species in modem Israel, most of which have been introduced in recent centuries. These plants obviously did not exist there in biblical times and were only recently introduced from Australia and South America, respectively. This article will study only the vegetables mentioned in the Bible and known to have existed in the old and new testament times. Since the first book devoted exclusively to biblical botany was that of Levinus Lemmens in 1566, the modem systematic study of biblical plants, began with F. Hasselquist, a student of Linnaeus, the founder of modem botany. In 1928, Immanuel Loew approached the subject differently, reviewing all known data pertaining to biblical plants. His work not only discussed biblical plants, but also plants in later Jewish literature, particularly the Talmud. The British scholar G. E. Post provided a broad field study of modem plants in Palestine, Syria, Lebanon, and Jordan. More recent major treatments of the subject include those of A. and H. Moldenke (1952), M. Zohary (1982), N. Hareuveni (1984), and Y. J. Choi(1996). Today, articles on specific biblical plants listed in the Bible can be found in any number of encyclopedias. This study attempts to provide a synthesis of the work of a number of scholars who studied the vegetables and plants mentioned in the Bible. As a preliminary study on the culture of food in the biblical period, this study has focused on the identity and features of the vegetables of the Bible. In only a limited number of instances, because of the paucity of the informations and the broad and generic descriptions of the plants, we can't be certain about the identification of the vegetables named in the Bible. In many instances the traditions established by the Greek, Aramaic, and English translations are helpful, although sometimes they are misleading. This paper subdivides the vegetables into broad areas, the general vegetables and the flavoring herbs. Vegetables formed very important part of the diet in the biblical times. Two main types were used: those whose nutritious seeds could be easily stored and those which were eaten freshly gathered from gardens. Pulse seeds provided a useful source of vegetable protein, while fresh green vegetables were vitamin rich. Pulses could be eaten boiled, or their dried seeds could be ground up into flour and then made into nutritious soups. Fresh vegetables were eaten either raw or lightly cooked, usually by boiling in water. The general vegetables in the Bible are herbs(garden rocket), cucumber(snake cucumber), watermelon, leeks, chicory, and onions. Also the flavoring herbs in the Bible are rue, dill, cummin, black cummin, frankincense, cinnamon, cassia, myrrh, black mustard, coriander, mint, saffron, ginger grass, syrian hyssop, aloes(eagle wood), manna which have the flavor, aroma, and medical values.

Eine Untersuchung ueber das Haus als das Mitte des Lebens und seine philosophische Bedeutung (삶의 중심으로서의 집과 그 철학적 의미에 대한 연구)

  • Byun, Sun-Yong
    • Journal of Ethics
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    • no.77
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    • pp.191-215
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    • 2010
  • In dieser vorliegenden Arbeit wird die eigentliche Bedeutung des Hauses in bezug auf M. Heidegger, G. Bachelard und O. F. Bollnow analysiert. Bei Heidegger ist das Wohnen ein Wesensmerkmal des Menschen und das Haus ein Raum des Wohnens als das Pflegen und Schonen. Insbesondre Bachelard hat die schuetzend Funktion des Hauses und das Haus als das Glueck des Wohnens behauptet. Fuer Bollnow bedeutet das Haus eine Mitte des menschlichen Lebens und der Welt. Heidegger behauptet, dass wir so in der eigentlichen Not des Wohnens, d.h. in der Heimatlosigkieit sind. Wir muessen nach die Bedeutung des Hauses nicht als einen wirtschaftlichen Wert sondern als den eigentlichen Wohnraum denken und das Wohnen lernen. Bachelard weist darauf hin, dass das Haus als ein gluekliche Raum ein wesentliches Trauen voraussetzt. Und er versteht das Haus mit dem dialektischen Zusammenhang zwischen dem Haus und der Welt. Bollnow versucht ueber dem Raum in anthropologische Hinsicht zu betrachten. Er behauptet, dass der Mensch seine wesentlichen Bedeutung durch das Wohnen hat. Durch die Vermenschlichung des Raumes muss der Mensch die Verantwortlichkeit ueber den Raum uebernehmen. In der heutigen Gesellschaft bemessen das Haus sich nur mit einem wirtschaftlichen Wert. Wir muessen das Haus lesen, und die eigentliche Bedeutung des Hauses.

Differences in Attitudes Toward Society and Politics Between Liberals and Conservatives (한국 사회와 정부에 대한 태도에서 진보·보수 집단 간 차이)

  • Hyejeong Ahn;Minju Lee;Taeyun Jung
    • Korean Journal of Culture and Social Issue
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    • v.19 no.4
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    • pp.529-552
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    • 2013
  • This study examines how individual attitudes towards government and society are affected by political conservatism and liberalism. With survey data from 533 adults(M=249, F=284) who live in Seoul and surrounding areas, we examine the general attitudes towards society, expectation on general government and differences in attitudes towards the Roh Moo-hyun Gov't and the Lee Myung-bak Gov't between liberals and conservatives. Results show that liberals and conservatives vary by age and residence, whereas attitudes towards the general government and society are not. By contrast, liberals and conservatives are distinctly different evaluating governments with specific political tendency. Liberals are more positive to the Roh Moo-hyun Gov't than conservatives whereas the evaluation appears conversely on the Lee Myung-bak Gov't. These differences of attitudes are more distinctive when dividing liberals and conservatives by subjective judgement and political party preference than conceptual measurement of political ideology. These findings suggest that political conservatism and liberalism is the factor that has powerfully affected individual attitudes in the specific political context rather than a consistent ideological framework in Korean society.

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