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http://dx.doi.org/10.15433/ksmb.2022.14.1.001

Effect of Culture Media on Production of Biomass, Fatty Acid, and Carotenoid in a Newly Isolated Mychonastes sp.  

Yim, Kyung June (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Jang, Hyun-Jin (Laboratory of Chemical Biology and Genomics, Korea Research Institute of Bioscience and Biotechnology)
Park, Yeji (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Nam, Seung Won (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Hwang, Byung Su (Plant Research Department, Nakdonggang National Institute of Biological Resources)
Jung, Ji Young (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Lee, Chang Soo (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Kim, Z-Hun (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.14, no.1, 2022 , pp. 1-8 More about this Journal
Abstract
This study examined the growth, fatty acid (FA) content, and carotenoids of a newly isolated freshwater microalga, Mychonastes sp. 246, in various culture media. The appropriate temperature and light intensity for culturing Mychonastes sp. 246 were determined as 18℃-22℃ and 200-250 µmol/m2/s using a high throughput photobioreactor. The microalgal cells were cultivated in 0.5 L bubble column photobioreactors using BG11, Bold's Basal media, and f/2 media. According to the growth results of the microalgae, BG11, among the tested media, showed the highest biomass concentrations (3.5 ± 0.1 g/L in 10 d). To enhance the biomass growth of the microalgae, the N:P ratio in BG11 was manipulated from 45:1 to 7:1 based on the stoichiometric cell composition. The biomass concentrations of Mychonastes sp. 246 grown on the manipulated BG11 (MBG) increased to 38% (4.6 ± 0.3 g/L in d) compared with the original BG11 (3.3 g/L). The FA content of the microalgae grown on the MBG was lower (8.4%) than that of the original BG11 (10.1%) while the FA compositions did not exhibit any significant differences. Furthermore, three kinds of carotenoids were identified in Mychonastes sp. 246, zeaxanthin, lutein, and β-carotene. These results suggest an effective strategy for increasing biomass concentrations, FA content, and carotenoids of microalgae by performing a simple N:P adjustment in the culture media.
Keywords
microalgae; Mychonastes; culture media; fatty acid; carotenoid;
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