• 약학회지
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• 제12권3_4호
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• pp.59-64
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• 1968

Silica gel column chromatography of the ether extract of the root of Angelica Koreana $M_{AXIMOWICZ}$ (Umbelliferae) gave five kinds of crystalline products of coumarin. The following kinds of furocoumarins were identified by UV, IR, NMR spectra and physico-chemical tests. iso-imperatorin m.p. 108-$109^{\circ}$ $C_{16}H_{14}O_{4}$ oxypeucedanin m.p. 142-$143^{\circ}$ $C_{16}H_{14}O_{5}$ prangolarine m.p. 104-$105^{\circ}$ $C_{16}H_{14}O_{5}$ imperatorin m.p. 100-$101^{\circ}$ $C_{16}H_{14}O_{4}$ These analyses also showed that white needles m.p. 129-$130^{\circ}$ were likely to be a furocoumarin. Besides, the methanol extract of the root was found to contain sucrose.

• Journal of Plant Biology
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• 제36권2호
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• pp.171-176
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• 1993

Protein kinase C, a protein related in PI cascade, was partially purified from the cytosol protein of etiolated plants of Glycine max by DEAE-52 cellulose chromatography and phenylsepharose chromatography. When the DEAE column was eluted with 0-0.8 M linear gradient KCl, tow fractions were found that increased the phosphorylation of histon H1 about five and nine-fold in the presence of 5 $\mu\textrm{g}$/mL phosphatidylserine and 0.5 $\mu\textrm{g}$/mL diolein, respectively. These fractions were used as DEAE pool. The reaction eluted with relatively high concentration of KCl was loaded on phyenylsepharose column with 5 mM CaCl2 and eluted with 1 mM EGTA. A fraction contained the protein kinase C, which increased the phosphorylation of the histon H1 was fractionated. To determine the molecular weight of PKC, the fraction eluted from phenylsepharose column was analyzed by 5~15% polyacrylamide gel electrophoresis after concentrated with the Amicon membrane (YM10). That revealed two bands corresponding to 60 and 65 kGy by silver staining of the gel, respectively.

• East Asian mathematical journal
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• 제38권5호
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• pp.549-581
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• 2022

Let M be a semi-invariant submanifold with almost contact metric structure (𝜙, 𝜉, 𝜂, g) of codimension 3 in a complex space form M_n+1(c), c≠ 0. We denote by S and R_𝜉 be the Ricci tensor of M and the structure Jacobi operator in the direction of the structure vector 𝜉, respectively. Suppose that the third fundamental form t satisfies dt(X, Y) = 2𝜃g(𝜙X, Y) for a certain scalar 𝜃(≠ 2c) and any vector fields X and Y on M. In this paper, we prove that M satisfies R_𝜉S = SR_𝜉 and at the same time R_𝜉𝜙 = 𝜙R_𝜉, then M is a Hopf hypersurface of type (A) provided that the scalar curvature s of M holds s - 2(n - 1)c ≤ 0.

• Journal of Mechanical Science and Technology
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• 제16권11호
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• pp.1420-1427
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• 2002

The Monkman-Grant (M-G) and its modified parameters were evaluated for type 316LN and modified 9Cr-Mo stainless steels prepared with minor element variations. Several sets of creep data for the two alloy systems were obtained by constant-load creep tests in 550～650$\^{C}$ temperature range. The M-G parameters, m, m', C, and C' were proposed and discussed for the two alloy systems. The m value of the M-C relation was 0.90 in type 316LN steel and 0.84 in modified 9Cr-Mo steel. The m' value of the modified relation was 0.94 in type 316LN steel and 0.89 in 9Cr-Mo steel. Although creep fracture modes and creep properties between type 316LN and modified 9Cr-Mo steels showed a basic difference, the M-G and its modified relations demonstrated linearity quite well. The m' of modified relation almost overlapped regardless of the creep testing conditions and chemical variations in the two alloy systems, and the parameter m' was closer to unity than that of the M-G relation.

• Archives of Pharmacal Research
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• 제27권8호
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• pp.863-866
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• 2004

The effect of substance P (Sub P) injected intrathecally (I.t.) on c-fos mRNA expression in vari-ous tissues was examined in the present study. We found that a single administration of Sub P(0.5 nM) caused an increase of the c-fos mRNA level in the hypothalamic-pituitary-adrenal(HPA) axis, hippocampus, and spinal cord. The time-course study showed that c-fos mRNA level was maximal at 10 min and began to decrease 30 min after the Sub P injection in all tis-sues, and the Sub P-induced increase of the c-fos mRNA level was returned to the control level 1 h after the injection. The kinetics of the c-fos mRNA expression in mice that were repeatedly injected with Sub P (every 30 min interval up to 4 times) were different in the HPA axis, hippocampus, and spinal cord. The increased c-fos mRNA level in the hypothalamus and the spinal cord induced by I.t. injected Sub P remained at a high level. In the pituitary gland, adrenal gland, and hippocampus, the increased level of c-fos mRNA expression gradually returned to the control level during the repeated substance P injections up to 4 times. Our results suggest that spinally injected Sub P-induced pain stress increases c-fos mRNA expres-sion in the spinal cord, hippocampus, and HPA axis. In mice repeatedly injected with Sub P, the kinetics of c-fos mRNA appear to be different varied from tissue to tissue.

• 한국식품영양과학회지
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• 제38권6호
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• pp.667-673
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• 2009

본 연구는 단백다당체 함유 차가버섯 균사체 추출물 보강이 고지방식이로 비만이 유도되어 2형 당뇨 특성을 나타내는 C57BL/6J mice에서 지질수준 개선 및 혈당조절에 미치는 영향을 알아보고자 하였다. 실험동물은 정상 대조군 (N-C), 고지방 대조군(H-C), 고지방식이+저농도 균사체 추출물 투여군(M150L, 1 mL/kg bw, ${\beta}$-glucan 1.303 mg), 고지방식이+균사체 추출물 투여군(M150, 2 mL/kg bw, ${\beta}$-glucan 2.606 mg), 고지방식이+고순도 단백다당체 투여군(PP, 200 mg/kg bw), 고지방식이+메시마 투여군(M, 200 mg/kg bw) 6군으로 나누어 사육하였으며 연구결과는 다음과 같다. 부고환 지방은 차가버섯 균사체 추출물 투여 농도의존적으로 유의적인 감소를 나타냈다. 혈중 중성지방 및 VLDL에서 차가버섯 균사체 투여군(M150)은 고지방 대조군(H-C)보다 각각 유의적으로 감소하였고, 간 조직의 중성지방 농도는 고지방 대조군(H-C)보다 차가버섯 균사체 투여 실험군(M150L, M150)이 각각 26.8%, 30.9%, 총 콜레스테롤은 각각 65.5%, 61.9% 낮아져 유의적으로 감소하였다. 경구당부하 검사 결과 포도당 투여 3시간 후의 혈당이 고지방 대조군(H-C)보다 저농도 차가버섯 균사체 투여군(M150L) 4.9%, 차가버섯 균사체 투여군(M150) 9.5% 감소하였다. 혈당반응면적 또한 차가버섯 균사체 투여군(M150)에서 유의한 수준으로 낮았다. 혈중 인슐린 및 HOMA-IR 수준은 차가버섯 균사체 투여 실험군(M150L, M150)에서 고지방 대조군(H-C)보다 유의적으로 감소되었다. 간 중 글리코겐은 고지방 대조군(H-C)에서 글리코겐 합성이 저하된 반면 차가버섯 균사체 투여 실험군(M150L, M150)에서는 유의적으로 증가하였다. 혈중 아디포넥틴은 고지방 대조군(H-C)보다 차가버섯 균사체 투여군(M150)에서 유의적으로 높아졌다. 결론적으로, 단백다당체 함유 차가버섯 균사체 추출물이 식이 유도 비만/당뇨 모델인 C57BL/6J mice의 지질수준 개선 및 혈당조절에 긍정적인 영향을 미치고 있어 이를 이용한 기능성식품으로의 개발 가능성이 높다고 사료된다.

• Bulletin of the Korean Chemical Society
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• 제24권6호
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• pp.832-836
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• 2003

To extend the knowledge of triple bonding between group 6 transition metal and heavier group 14 elements, the structural and bonding aspects of ($η^5-C_5H_5$)$(CO)_2$M≡ER (M = Cr, Mo, W; E = Si, Ge, Sn, Pb) are investigated by hybrid density functional calculations at the B3PW91 level. Substituent effects are also investigated with R = H, Me, $SiH_3$, Ph, $C_6H_3-2,6-Ph_2$, $C_6H_3-2,6-(C_6H_2-2,4,6-Me_3)_2$, and $C_6H_3-2,6-(C_6H_2-2,4,6- iPr_3)_2$.

• Bulletin of the Korean Chemical Society
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• 제30권4호
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• pp.791-793
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• 2009

Escherichia coli RNase P is composed of a large RNA subunit (M1 RNA) and a small protein subunit (C5 protein). Since both subunits are assembled in a 1:1 ratio, expression of M1 RNA and C5 protein should be coordinately regulated for RNase P to be efficiently synthesized in the cell. However, it is not known yet how the coordination occurs. In this study, we investigated how overexpression of C5 protein affects expression of the rnpB gene encoding M1 RNA, using a lysogenic strain, which carries an rnpB-lacZ transcription fusion. Primer extension analysis of rnpB-lacZ fusion transcripts showed that the overexpression of C5 protein increased the amount of the fusion transcripts, suggesting that rnpB expression increases with the increase of intracellular level of C5 protein.

• 대한수학회논문집
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• 제11권3호
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• pp.673-680
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• 1996

It is proved that $K(c_0,Y)$ is an M-ideal in $L(c_0,Y)$ if Y is a closed subspace of $c_0$. And a new direct proof of the fact that $K(L_1[0,1],\ell_1)$ is not an M-ideal in $L(L_1[0,1],\ell_1)$ is given.

• The Korean Journal of Physiology and Pharmacology
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• 제2권4호
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• pp.471-477
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• 1998

Cyclosporin A (CsA), a widely used immunosuppressant, is well known to cause nephrotoxicity and hypertension as major side effects. The present study was aimed at investigating the effects of CsA-pretreatment on the activities of cytosolic guanylate cyclase (cGC) in relation to the alteration of relaxant responses in the rat thoracic aorta. CsA $(10\;{\mu}M)-preincubation$ for 90 min significantly attenuated the vasodilatation induced by sodium nitroprusside (SNP), a cytosolic guanylate cyclase activator, shifting the dose-response curve to the right. The increase in cGMP contents induced by SNP was markedly attenuated by CsA. SNP ($1\;{\mu}M{\sim}\;mM$) increased the cGC activity dose-dependently, and the increase was completely abolished by CsA. CsA attenuated the SNP-induced cGC activation dose-dependently. The abolishing effect of CsA-pretreatment on the SNP-induced cGC activation was not affected by washing the preparation, suggesting that the inhibition is irreversible. When CsA was added simultaneously with SNP, cGC activation was not attenuated. 1-(5-isoquinolinylsulfonyl)-2-methyl piperazine (H-7), a protein kinase C (PKC) inhibitor, decreased SNP-induced cGC activation and blocked the CsA-attenuation of cGC activation. These results suggest that CsA directly inhibits cGC participating in the CsA-induced impairment of vasodilatation, and that PKC is involved in the inhibitory action of CsA on cGC.