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Effect of method of synthesis on antifungal ability of ZnO nanoparticles: Chemical route vs green route

  • Patino-Portela, Melissa C.;Arciniegas-Grijalba, Paola A.;Mosquera-Sanchez, Lyda P.;Sierra, Beatriz E. Guerra;Munoz-Florez, Jaime E.;Erazo-Castillo, Luis A.;Rodriguez-Paez, Jorge E.
    • Advances in nano research
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    • v.10 no.2
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    • pp.191-210
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    • 2021
  • To compare the antifungal effect of two nanomaterials (NMs), nanoparticles of zinc oxide were synthesized by a chemical route and zinc oxide-based nanobiohybrids were obtained using green synthesis in an extract of garlic (Allium sativum). The techniques of X-Ray Diffraction (XRD), Infrared (IR) and Ultraviolet Visible (UV-Vis) absorption spectroscopies and Scanning (SEM) and Transmission Electron Microscopies (TEM) were used to determine the characteristics of the nanomaterials synthesized. The results showed that the samples obtained were of nanometric size (< 100 nm). To compare their antifungal capacity, their effect on Cercospora sp. was evaluated. Test results showed that both nanomaterials had an antifungal capacity. The nanobiohybrids (green route) gave an inhibition of fungal growth of ~72.4% while with the ZnO-NPs (chemical route), inhibition was ~87.1%. Microstructural studies using High Resolution Optical Microscopy (HROM) and ultra-structural analysis using TEM carried out on the treated strains demonstrated the effect of the nanofungicides on the vegetative and reproductive structures, as well as on their cell wall. To account for the antifungal effect presented by ZnO-NPs and ZnO nanobiohybrids on the fungi tested, effects reported in the literature related to the action of nanomaterials on biological entities were considered. Specifically, we discuss the electrical interaction of the ZnO-NPs with the cell membrane and the biomolecules (proteins) present in the fungi, taking into account the n-type nature of the ZnO semiconductor and the electrical behavior of the fungal cell membrane and that of the proteins that make up the protein crown.

RELATION BETWEEN BLACK HOLE MASS AND BULGE LUMINOSITY IN HARD X-RAY SELECTED TYPE 1 AGNS

  • Son, Suyeon;Kim, Minjin;Barth, Aaron J.;Ho, Luis C.
    • Journal of The Korean Astronomical Society
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    • v.55 no.2
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    • pp.37-57
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    • 2022
  • Using I-band images of 35 nearby (z < 0.1) type 1 active galactic nuclei (AGNs) obtained with Hubble Space Telescope, selected from the 70-month Swift-BAT X-ray source catalog, we investigate the photometric properties of the host galaxies. With a careful treatment of the point-spread function (PSF) model and imaging decomposition, we robustly measure the I-band brightness and the effective radius of bulges in our sample. Along with black hole (BH) mass estimates from single-epoch spectroscopic data, we present the relation between BH mass and I-band bulge luminosity (MBH-MI,bul relation) of our sample AGNs. We find that our sample lies offset from the MBH-MI,bul relation of inactive galaxies by 0.4 dex, i.e., at a given bulge luminosity, the BH mass of our sample is systematically smaller than that of inactive galaxies. We also demonstrate that the zero point offset in the MBH-MI,bul relation with respect to inactive galaxies is correlated with the Eddington ratio. Based on the Kormendy relation, we find that the mean surface brightness of ellipticals and classical bulges in our sample is comparable to that of normal galaxies, revealing that bulge brightness is not enhanced in our sample. As a result, we conclude that the deviation in the MBH-MI,bul relation from inactive galaxies is possibly because the scaling factor in the virial BH mass estimator depends on the Eddington ratio.

Unicortical Bone Necrosis of the Fibula Free Flap Associated to the Fixation with a Nonlocking 2.0-mm Reconstruction Plate and Screws

  • Pereira, Gustavo N.;Ribeiro, Diogo;Saraiva, Luis;Freitas, Hugo;Santos, Ana R.
    • Archives of Plastic Surgery
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    • v.49 no.3
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    • pp.413-417
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    • 2022
  • The authors present a unique case of osteonecrosis of a cortical half of a fibula free flap that has not been reported in the literature yet. This complication was associated with the impairment of the vascularization of periosteum in the cortical half of fibula that was fixated with a nonlocking reconstructive 2.0-mm plate and screws but other factors could have been involved. The patient was submitted to excision of a cemento-ossifying fibroma that resulted in a left hemimaxilectomy mesoinfrastructure defect classified as the Cordeiro type 2B. The 42-year-old female patient was submitted to reconstruction with an osteomusculocutaneous fibula free flap plus a segment of fibula graft. The two bone segments of the free flap used to reconstruct the anterior and left alveolar crest were fixated with a reconstructive 2.0-mm plate of matrixMANDIBLE system. The only reported complication was an oronasal fistula that healed with conservative treatment and the referred osteonecrosis of the external cortical half of the fibula free flap with plate exposure at 2.5 years postoperatively. Surgical excision of the osteonecrosed cortical half of the fibula with the plate and screws was performed, while the other cortical underwent bone union as corroborated by computed tomography scans.

Investigating the acceptance of the reopening Bataan nuclear power plant: Integrating protection motivation theory and extended theory of planned behavior

  • Ong, Ardvin Kester S.;Prasetyo, Yogi Tri;Salazar, Jose Ma Luis D.;Erfe, Justine Jacob C.;Abella, Arving A.;Young, Michael Nayat;Chuenyindee, Thanatorn;Nadlifatin, Reny;Redi, Anak Agung Ngurah Perwira
    • Nuclear Engineering and Technology
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    • v.54 no.3
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    • pp.1115-1125
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    • 2022
  • Nuclear power plant (NPP) is currently considered as one of the most reliable power sources. However, 182 of them are considered decommissioned and inactive including the one in Bataan, Philippines. The aim of this study was to investigate the acceptance of the reopening of Bataan Nuclear Power Plant (BNPP) by integrating the Theory of Planned Behavior and Protection Motivation Theory. A total of 815 Filipinos answered an online questionnaire which consisted of 37 questions. The Structural Equation Modeling (SEM) indicated that knowledge towards nuclear power plants was the key factor in determining people's acceptance towards NPP reopening. In addition, knowing the benefits would lead to positive perceived behavioral control (PBC) and attitude towards intention. Results showed that PBC and attitude are mediators towards the acceptance of people regarding the reopening of BNPP. If an individual's knowledge gravitates towards the perceived risk, then this can lead to the negative acceptance of the NPP reopening. On the other hand, if an individual's knowledge gravitates towards the perceived benefits, then this will lead to positive acceptance. This study is the first study that explored the acceptance of the reopening BNPP. Finally, the study's model construct would also be very beneficial for researchers, government, and even private sectors worldwide.

Microshear bond strength of dual-cure resin cement in zirconia after different cleaning techniques: an in vitro study

  • Atoche-Socola, Katherine Joselyn;Arriola-Guillen, Luis Ernesto;Lopez-Flores, Ana Isabel;Garcia, Isadora Martini;Huertas-Mogollon, Gustavo;Collares, Fabricio Mezzomo;Leitune, Vicente Castelo Branco
    • The Journal of Advanced Prosthodontics
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    • v.13 no.4
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    • pp.237-245
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    • 2021
  • PURPOSE. This study aimed to compare the microshear bond strength (µSBS) of dual-cure resin cement in CAD-CAM zirconia after different cleaning techniques. MATERIALS AND METHODS. Fifty discs of zirconia-based ceramic from Ivoclar Vivadent were embedded in acrylic resin. The discs were divided into five groups according to the cleaning methods used: Group 1: drying with spraying + sandblasting with Al2O3; Group 2: washed with water and dried with spraying + sandblasting with Al2O3;Group 3: washed with distilled water and dried with spraying + sandblasting with Al2O3 + zirconium oxide (Ivoclean); Group 4: washed with distilled water and dried with spraying + sandblasting with Al2O3 + potassium hydroxide (Zirclean); and Group 5: washed with distilled water and dried with spraying + sandblasting with Al2O3 + 1% NaClO. All of the groups were contaminated with artificial saliva for 1 minute and then cleaned. Statistical analyses were performed using ANOVA and Tukey's tests. RESULTS. There were statistically significant differences among all groups for µSBS (P < .05). The group treated with zirconium oxide (Group 3) showed the highest µSBS (18.75 ± 0.23 MPa). CONCLUSION. When applied to zirconia, the cleaning methods affected the bonding with resin cement differently.

Ultrasound and clinical findings in the metacarpophalangeal joint assessment of show jumping horses in training

  • Yamada, Ana Lucia M.;Pinheiro, Marcelo;Marsiglia, Marilia F.;Hagen, Stefano Carlo F.;Baccarin, Raquel Yvonne A.;da Silva, Luis Claudio L.C.
    • Journal of Veterinary Science
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    • v.21 no.3
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    • pp.21.1-21.14
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    • 2020
  • Background: Physical exercise is known to cause significant joint changes. Thus, monitoring joint behavior of athletic horses is essential in early disorders recognition, allowing the proper management. Objectives: The aims of this study were to determine the morphological patterns, physical examination characteristics and ultrasound findings of show jumping horses in training and to establish a score-based examination model for physical and ultrasound follow-ups of metacarpophalangeal joint changes in these animals. Methods: A total of 52 metacarpophalangeal joints from 26 horses who were initially in the taming stage were evaluated, and the horses' athletic progression was monitored. The horses were evaluated by a physical examination and by B-mode and Doppler-mode ultrasound examinations, starting at time zero (T0), which occurred concomitantly with the beginning of training, and every 3 months thereafter for a follow-up period of 18 months. Results: The standardized examination model revealed an increase in the maximum joint flexion angles and higher scores on the physical and ultrasound examinations after scoring was performed by predefined assessment tools, especially between 3 and 6 months of evaluation, which was immediately after the horses started more intense training. The lameness score and the ultrasound examination score were slightly higher at the end of the study. Conclusions: The observed results were probably caused by the implementation of a training regimen and joint adaptation to physical conditioning. The joints most likely undergo a pre-osteoarthritic period due to work overload, which can manifest in a consistent or adaptive manner, as observed during this study. Thus, continuous monitoring of young athlete horses by physical and ultrasound examinations that can be scored is essential.

Comparison of Outcomes in Immediate Implant-Based Breast Reconstruction: Acellular Dermal Matrix versus Inferior Dermal Flap

  • Ribeiro, Luis Mata;Meireles, Rita P.;Brito, Iris M.;Costa, Patricia M.;Rebelo, Marco A.;Barbosa, Rui F.;Choupina, Miguel P.;Pinho, Carlos J.;Ribeiro, Matilde P.
    • Archives of Plastic Surgery
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    • v.49 no.2
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    • pp.158-165
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    • 2022
  • Background Implant-based breast reconstruction has evolved tremendously in the last decades, mainly due to the development of new products and techniques that make the procedure safer and more reliable. The purpose of this study was to compare the outcomes in immediate one-stage breast reconstruction between acellular dermal matrix (ADM) and inferior dermal flap (IDF). Methods We conducted a retrospective comparative study of patients submitted to immediate breast reconstructions with an anatomical implant and ADM or IDF in a single center between 2016 and 2018. Outcomes evaluated included major complications, early complications, reinterventions, readmissions, and reconstruction failure. Simple descriptive statistics and univariate analysis were performed. Results A total of 118 breast reconstructions (85 patients) were included in the analysis. Patients in the IDF group had a higher body mass index (median = 27.0) than patients in the ADM group (median = 24). There were no statistically significant differences among both groups regarding immediate major complication, early complications, readmissions, and reinterventions. Conclusion There are no significant differences in complications between the ADM and IDF approach to immediate implant breast reconstruction. In patients with higher body mass index and large, ptotic breasts, we recommend an immediate implant reconstruction with IDF.

Clinical effects of different prescriptions on the inclination of maxillary and mandibular incisors by using passive self-ligating brackets

  • Savoldi, Fabio;Sangalli, Linda;Ghislanzoni, Luis T. Huanca;Dalessandri, Domenico;Gu, Min;Mandelli, Gualtiero;Paganelli, Corrado
    • The korean journal of orthodontics
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    • v.52 no.6
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    • pp.387-398
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    • 2022
  • Objective: Controlling the incisal inclination is fundamental in orthodontics. However, the relationship between the inclination prescription and its clinical outcome is not obvious, and the incisal inclination changes generated by different bracket prescriptions were investigated. Methods: Twenty-eight non-extraction dental Class II patients (15 females, 13 males; mean age = 12.9) were retrospectively analyzed. Patients were treated using passive self-ligating fixed appliances with three inclination prescriptions for maxillary incisors (high, standard, low), and two for mandibular incisors (standard, low). Clinical outcomes were compared among different prescriptions, and regression analysis was used to explain the effects of bracket prescriptions and to understand the prescription selection criteria (α = 0.05). Results: For maxillary central incisors, low and high prescriptions were related to linguoversion (p = 0.046) and labioversion (p = 0.005), respectively, while standard prescription maintained the initial dental inclination. Maxillary lateral incisors did not show significant changes. For mandibular incisors, low prescription led to linguoversion (p = 0.005 for central incisors, p = 0.010 for lateral incisors), while standard prescription led to labioversion (p = 0.045 for central incisors, p = 0.005 for lateral incisors). The factors affecting inclination changes were the imposed change and selected prescription, while prescription selection was influenced by the initial dental inclination and initial intercanine distance. Conclusions: The direction of correction of incisal inclination can be controlled by choosing a certain prescription, but the final inclination may show limited consistency with it. The amount of imposed inclination change was the most relevant predictor of the clinical outcome.

Validation and comparison of volume measurements using 1 multidetector computed tomography and 5 cone-beam computed tomography protocols: An in vitro study

  • Juliana Andrea Correa, Travessas;Alessandra Mendonca, dos Santos;Rodrigo Pagliarini, Buligon;Nadia Assein, Arus;Priscila Fernanda Tiecher, da Silveira;Heraldo Luis Dias, da Silveira;Mariana Boessio, Vizzotto
    • Imaging Science in Dentistry
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    • v.52 no.4
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    • pp.399-408
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    • 2022
  • Purpose: The purpose of this study was to compare volume measurements obtained using 2 image software packages on Digital Imaging and Communications in Medicine (DICOM) images acquired from 1 multidetector computed tomography and 5 cone-beam computed tomography devices, using different protocols for physical volume measurements. Materials and Methods: Four pieces of bovine leg were prepared. Marrow was removed from 3 pieces, leaving cortical bone exposed. The resulting space of 1 piece was filled with water, another was filled with propylene glycol, and the third was left unfilled. The marrow in the fourth sample was left fully intact. Volume measurements were obtained after importing DICOM images into the Dolphin Imaging 11.95 and ITK-SNAP software programs. Data were analyzed using 3-way analysis of variance with a generalized linear model to determine the effects of voxel size, software, and content on percentage mean volume differences between tomographic protocols. A significance level of 0.05 was used. Results: The intraclass correlation coefficients for intraobserver and interobserver reliability were, respectively, 0.915 and 0.764 for the Dolphin software and 0.894 and 0.766 for the ITK-SNAP software. Three sources of statistically significant variation were identified: the interaction between software and content (P=0.001), the main effect of content (P=0.014), and the main effect of software (P=0.001). Voxel size was not associated with statistically significant differences in volume measurements. Conclusion: Both content and software influenced the accuracy of volume measurements, especially when the content had gray values similar to those of the adjacent tissues.

Comparative clinical and radiologic evaluation between patients undergoing standard reversed shoulder arthroplasty or bony increased offset

  • Tiago Amorim-Barbosa;Ana Ribau;Helder Fonte;Luis Henrique Barros;Rui Claro
    • Clinics in Shoulder and Elbow
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    • v.26 no.1
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    • pp.3-9
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    • 2023
  • Background: Modifications of the medialized design of Grammont-type reverse shoulder arthroplasty (RSA) using a bony increased offset (BIO-RSA) has shown better clinical results and fewer complications. The aim of this study is to compare the clinical results, complications, and radiological outcomes between patients undergoing standard RSA and BIO-RSA. Methods: A retrospective review was performed of 42 RSA procedures (22 standard RSA and 20 BIO-RSA). With a minimum of 1 year of follow-up, range of motion (ROM), Constant shoulder score (CSS), visual analog scale (VAS), and subjective shoulder score (SSS) were compared. Radiographs and computed tomography (CT) scan were examined for scapular notching, glenoid and humeral fixation, and graft healing. Results: At a mean follow-up of 27.6 months (range, 12-48 months), a significant difference was found for active-internal rotation (P=0.038) and for passive-external rotation (P=0.013), with better results in BIO-RSA. No other differences were found in ROM, CSS (P=0.884), VAS score, and SSS. Graft healing and viability were verified in all patients with CT scan (n=34). The notching rate was 28% in the standard RSA group and 33% in the BIO-RSA group, but the standard RSA had more severe notching (grade 2) than BIO-RSA (P=0.039). No other significative differences were found in glenoid and humeral fixation. Conclusions: Bone-graft lateralization is associated with better internal and external rotation and with less severe scapular notching compared to the standard RSA. Integration of the bone graft occurs effectively, with no relevant changes observed on radiographic evaluation. Level of evidence: III.