• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1669-1679
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• 2015

The quest to broaden the narrow range of feed ingredients available to pig producers has prompted research on the use of low cost, unconventional feedstuffs, which are typically fibrous and abundant. Maize cobs, a by-product of a major cereal grown worldwide, have potential to be used as a pig feed ingredient. Presently, maize cobs are either dumped or burnt for fuel. The major challenge in using maize cobs in pig diets is their lignocellulosic nature (45% to 55% cellulose, 25% to 35% hemicellulose, and 20% to 30% lignin) which is resistant to pigs' digestive enzymes. The high fiber in maize cobs (930 g neutral detergent fiber/kg dry matter [DM]; 573 g acid detergent fiber/kg DM) increases rate of passage and sequestration of nutrients in the fiber reducing their digestion. However, grinding, heating and fermentation can modify the structure of the fibrous components in the maize cobs and improve their utilization. Pigs can also extract up to 25% of energy maintenance requirements from fermentation products. In addition, dietary fiber improves pig intestinal health by promoting the growth of lactic acid bacteria, which suppress proliferation of pathogenic bacteria in the intestines. This paper reviews maize cob composition and the effect on digestibility of nutrients, intestinal microflora and growth performance and proposes the use of ensiling using exogenous enzymes to enhance utilization in diets of pigs.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.30 no.4
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• pp.18-27
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• 1998

Biopulping is defined as the treatment of wood chips with lignin-degrading fungi prior to pulping. Fungal pretreatment prior to mechanical pulping reduces electrical energy requirements during refining or increases mill throughput, improves paper strength, reduces the pitch content, and reduces the environmental impact of pulping. Our recent work involved scaling up the biopulping process towards the industrial level, investigating both the engineering and economic feasibility. We envision the process to be done in either a chip-pile or silo-based system for which several factors need to be considered: the degree of decontamination, a hospitable environment for the fungus, and the overall process economics. Currently, treatment of the chips with low-pressure steam is sufficient for decontamination and a simple, forced ventilation system maintains the proper temperature, humidity, and moisture conditions, thus promoting uniform growth of the fungus. The pilot-scale trial resulted in the successful treatment of 4 tons of wood chips (dry weight basis) with results comparable to those on a laboratory. Larger, 40-ton trials were also successful, with energy savings and paper properties comparable with the laboratory scale. The overall economics of the process also look very favorable and can result in significant annual savings to the mill. Although the current research has focused on biopulping for mechanical pulping, it is also beneficial for sulfite chemical pulping and some applications to recycled fiber have been investigated.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.4
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• pp.303-308
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• 2000

The objective of this experiment was to evaluate chemical composition, dry matter(DM) digestibility, DM intake, relative feed value(RFV) and hay grade of imported roughage which was collected by wholesale dealer at Chungnam province in 1999. Experimental roughages includes 8 kinds of imported hays(com stover bale, tall fescue straw, green cell, bermudagrass straw, reed carnarygrass straw, alfalfa bale, sugarcane bale and oat hay) and mixture hay(control) which was harvested at Chungnam National University experimental field. Compared with mixture hay(control) except for alfalfa bale, crude protein of most imported roughage was low, but NDF, ADF, cellulose and lignin compound were high. Futhermore, DM digestibility, DM intake, RFV and hay grade of imported roughage except for alfalfa bale was significantly lower than those of mixture hay(control)(P<0.05). Therefore, it is necessary to evaluate the economic value in the aspect of quality for the imported roughages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.297-302
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• 1995

The objective of this experiment was to compare the dry matter intake, nutrients digestibility, nitrogen and energy utilization of hehage among the orchardgrass(OG) pure, orchardgrass(OG)-red clover(RC) mixture and complex rnixtures(C. mix) by Korean native goat. The results obtained were surnmerized as follows: 1. Crude protein(CP) content was higher in OG-RC and C. Mix diets than in OG pure diets(P<0.05). NDF content was higher in OG pure diets than other diets(P<0.05). But ADF, Lignin contents and gross energy did not differ among all diets. 2. Dry matter intake by Korean native goat tend to be high in OG-RC diets, and low in OG pure diets. But there was no significant difference statistically. Dry matter and cellular constituents digestibilities were higher for OG-RC and C. Mix diets than OG pure diet, but CP and NDF digestibilities were no significant difference among diets. 3. The amount of nitrogen consumed amount by Korean native goat in the OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal nitrogen loss, Apparrently digested nitrogen and retained nitrogen percents were not significant difference among diets. 4. The amount of energy consumed by Korean native goat in OG-RC diet was higher than other diets. But, due to largely relative urinary and fecal energy loss, digestable energy percent was lower than C. Mix diets. But.metabolic energy percent was no significant difference among diets. Therefore, there was no significant differences among sward types for the utilization of nitrogen and energy by Korean native goats.

• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1069-1076
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• 2010

A novel laccase from Tricholoma mongolicum was purified by using a procedure that entailed ion-exchange chromatographies on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and FPLC-gel filtration on Superdex 75. The purified enzyme was obtained with a specific activity of 1,480 U/mg-protein and a final yield of 15%. It was found to be a monomeric protein with a molecular mass of 66 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was GIGPVADLYVGNRIL, similar to some but also different to other mushroom laccases. The optimum pH and temperature for the purified enzyme were pH 2 to pH 3 and $30^{\circ}C$, respectively. It displayed a low $K_m$ toward 2,7-azinobis (3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) and high $k_{cat}/K_m$ values. The purified laccase oxidized a wide range of lignin-related phenols, but exerted maximal activity on ABTS. It was significantly inhibited by $Hg^{2+}$ ions, and remarkably stimulated by $Cu^{2+}$ ions. It inhibited HIV-1 reverse transcriptase and proliferation of hepatoma HepG2 cells and breast cancer MCF7 cells with an $IC_{50}$ of 0.65 ${\mu}M$, 1.4 ${\mu}M$, and 4.2 ${\mu}M$, respectively, indicating that it is also an antipathogenic protein.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.31 no.4
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• pp.41-48
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• 1999

This study was intended to investigated the effect of pulp type and intital calendering on the properties of recycled fiber. As the number of recycling is increased, at least by the fourth recycling , the freeness of the SW-BKP and HW-BKP increased. And then that decrease after the fifth recycling. Especially the freeness of CTMP increased more rapidly than any other pulp in first recycling. And there was no difference of freeness in all kind of pulp by initial calendering . The speed of dewatering in making sheet consistetly became slow by proceeding recycling on condition of all calendering. and the order of the speed of dewatering was as followed. CTMP>HW-BKP>SW-BKP. However in case of HW-BKP, which was low -yield pulp, the tendency of the speed of dewatering was similar to that of CTMP which was high-yield pulp and had high content lignin . There was no change of fiber length and no effect of calendering by recycling of HW-BKP and CTMP. Howeverfiber length of SW-BKP generally decreased, especially in calendering of high temperature and high pressure conditions. The WRV of SW-BKP diminished by proceeding recycling but that of HW-BKP and CTMP diminished in the first recycling and then there was no change of the WRV of HW-BKP and CTMP. The contents of fines of HW-BKP consistently decreased by the fourth recycling and then increased in the fifth recycling . On the other hand there was no tendency in the content of fines of SW-BKP and CTMP by the number of recycling.

• KSBB Journal
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• v.10 no.2
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• pp.183-190
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• 1995

The effects of enzymatic pretreatments on the bleaching of kraft pulp were studied. The kappa number of pulp samples which represents the lignin content of pulp decreased by 25.2% by the pretreatments with xylanase(EC 3.2.1.8, Pulpzyme HB) while it decreased by 13.7% without enzyme pretreatments after the extraction of the pretreated pulp samples in 1N NaOH. To enhance the effects of enzymatic pretreatment on the bleaching of kraft pulp, phenols were used as radical carriers with the simultaneous use of peroxidase(EC 1.11.1.7, Novozyme 502), $H_2O_2$, and xylanase. Guaiacol (1mM) was most effective by decreasing the kappa number by 29.6% when a low initial concentration of $H_2O_2$ (0.1mM) was used. The use of either a higher initial concentration of $H_2O_2$ or phenols lacking electron donating substituents such as phenol and p-chloyophenol, however, decreased the efficiency of enzymatic pretreatment indicating that the production rate and the stability of phenolic radicals are important parameters.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.516-521
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• 2002

The objective of this study was to compare two varieties of Napier grass (Bana Napier grass vs French Cameroon Napier grass) and to determine whether feed intake, digestibility, average daily gain (ADG) and milk yield of lactating Friesian cows from fresh cut Bana Napier grass was greater than from French Cameroon Napier grass, using a completely randomized design. Results show that Bana Napier grass had similar percent dry matter (DM), ash and gross energy (GE) to French Cameroon. Bana grass had higher percent crude protein (CP) and lower fiber fractions, acid detergent fibre (ADF), neutral detergent fibre (NDF) and lignin compared to French Cameroon. Overall the forage quality was marginally higher in Bana Napier grass compared to French Cameroon. The DM and NDF intake expressed as a percentage of body weight (BW) were similar in both Napier grass types. Both grasses had similar digestible DM and energy. Bana had higher digestible CP but lower digestible ADF and NDF than French Cameroon. Bana Napier was not different from French Cameroon when fed as a sole diet to lactating cows in terms of low DM intake, milk yield and a loss of BW and condition. To improve the efficient utilization of both Napier grass varieties, a supplement capable of supplying 1085-1227 g CP/d and 17.0-18.0 Mcal ME/d is required for cows to support moderate gains 0.22 kg/d and 15 kg 4% fat corrected milk/d.

• Journal of the Korean Wood Science and Technology
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• v.23 no.4
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• pp.27-32
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• 1995

To understand whether ligninolytic enzyme catalyze polymerization or depolymerization of the high molecular weight (HMW) lignin, the action of laccase and Mn-peroxidase (MnP) towards commercial ligninsulfonates (LS) was examined in various conditions of pH and cosubstrates. Polymerization occurred when LS was incubated with laccase at pH 6.0. In contrast, the high molecular weight portions were significant1y reduced at pH 4.5, especially when glucose was added. When LS was treated with MnP at pH 4.5, compounds of low molecular weight were produced. In particular, when cellobiose was added to Mn-P reaction mixture, the breakdown of LS was observed. In conclusion, degradation of LS by laccase and MnP occurred primarily at pH 4.5 where-as polymerization of LS was dominant at pH 6.0. Color index, however, was not greatly changed in the degradation mixtures of LS.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.517-522
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• 2007

The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b ($1-e^{-ct}$). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p<0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p<0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.