• Title/Summary/Keyword: Low Level Laser Therapy

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The Effect of 3mW Semiconductor Laser Irradiation on Cell Proliferation (세포 증식에서 3mW 반도체 레이저 조사가 미치는 효과)

  • Cheon, Min-Woo;Park, Yong-Pil;Lee, Ho-Shik;Kim, Tae-Gon;Kim, Young-Pyo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.572-573
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    • 2010
  • This paper performed the basic study for fabricating the low level laser therapy apparatus, and one of the goals of this paper was to make this apparatus used handily. The equipment was fabricated using the 655nm laser diode and microcontroller unit and designed to enable us to control light irradiation time, and reservation.. In case of cell proliferation experiment, each experiment was performed to irradiation group and non-irradiation group for bome marrow cell. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring.

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Influence of Diode Laser (808 nm) on a Rat Anterior Cruciate Ligament Transection Model of Osteoarthritis (전십자인대 단열을 통한 랫드 골관절염모델에서 다이오드레이져 (808 nm)의 영향)

  • Park, Seongkyu;Minar, Maruf;Hwang, Yawon;Kim, Somin;Park, Minhyeok;Choi, Seok-Hwa;Kim, Gonhyung
    • Journal of Veterinary Clinics
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    • v.30 no.5
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    • pp.346-352
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    • 2013
  • The study was aimed to investigate the influence of diode laser on osteoarthritis (OA) of stifle joint induced by anterior cruciate ligament transection (ACLT). Sixty 10-week-old male Sprague-Dawley rats were used in this study. Right stifle joint was operated to create ACLT or sham. There were five study groups: control, Sham, ACLT, ACLT + Laser irradiation (ACLT+L) and ACLT + meloxicam administration (ACLT+M). Low-level laser therapy (LLLT) was applied at the operated stifle joint twice a week using an 808-nm indium-gallium-arsenide (InGaAs) diode laser during 8-week experimental period. Radiographical, gross morphological and histopathological findings were examined at 2, 4 and 8 weeks post-surgery. Radiography, CBC and chemistry tests showed no significant difference between groups. ACLT+L group showed remarkable cartilage damages compared with sham group morphologically and histopathologically at 2, 4 and 8 weeks after surgery. ACLT+M group also had more cartilage damages compared with sham group. Low-level laser therapy (LLLT) showed limitation to prevent progression of OA in the rat anterior cruciate ligament transection models; on the contrary it accelerated cartilage damage. It is assumed that the aggravating results of LLLT in this study might be due to excessive unstable movement of stifle joint from the pain-relieving effect of LLLT, rather than direct damaging effect of irradiation since LLLT did not affect cell viability.

Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts

  • Choi, Eun-Jeong;Yim, Ju-Young;Koo, Ki-Tae;Seol, Yang-Jo;Lee, Yong-Moo;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung;Kim, Tae-Il
    • Journal of Periodontal and Implant Science
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    • v.40 no.3
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    • pp.105-110
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    • 2010
  • Purpose: It has been reported that low-level semiconductor diode lasers could enhance the wound healing process. The periodontal ligament is crucial for maintaining the tooth and surrounding tissues in periodontal wound healing. While low-level semiconductor diode lasers have been used in low-level laser therapy, there have been few reports on their effects on periodontal ligament fibroblasts (PDLFs). We performed this study to investigate the biological effects of semiconductor diode lasers on human PDLFs. Methods: Human PDLFs were cultured and irradiated with a gallium-aluminum-arsenate (GaAlAs) semiconductor diode laser of which the wavelength was 810 nm. The power output was fixed at 500 mW in the continuous wave mode with various energy fluencies, which were 1.97, 3.94, and 5.91 $J/cm^2$. A culture of PDLFs without laser irradiation was regarded as a control. Then, cells were additionally incubated in 72 hours for MTS assay and an alkaline phosphatase (ALPase) activity test. At 48 hours post-laser irradiation, western blot analysis was performed to determine extracellular signal-regulated kinase (ERK) activity. ANOVA was used to assess the significance level of the differences among groups (P<0.05). Results: At all energy fluencies of laser irradiation, PDLFs proliferation gradually increased for 72 hours without any significant differences compared with the control over the entire period taken together. However, an increment of cell proliferation significantly greater than in the control occurred between 24 and 48 hours at laser irradiation settings of 1.97 and 3.94 $J/cm^2$ (P<0.05). The highest ALPase activity was found at 48 and 72 hours post-laser irradiation with 3.94 $J/cm^2$ energy fluency (P<0.05). The phosphorylated ERK level was more prominent at 3.94 $J/cm^2$ energy fluency than in the control. Conclusions: The present study demonstrated that the GaAlAs semiconductor diode laser promoted proliferation and differentiation of human PDLFs.

Current concepts of Laser dentistry (임상가를 위한 특집2 - Current concepts of Laser dentistry)

  • Eun, Hee-Jong
    • The Journal of the Korean dental association
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    • v.49 no.11
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    • pp.670-678
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    • 2011
  • Various sorts of dental devices for the dental treatment have been introduced and adopted during past several years. Dental Laser, among all devices, seems to be possible for applying to over the whole treatments and widely employed. Furthermore, this article is to introduce that Dental Laser is intended to care patients secured with the effectual treatment against Conventional procedures based upon its studies. The advantages of Dental Laser treatment-along with LLLT (Low Level Laser Therapy) effect, biostimulation effect, and minimal invasive technique - make the patients reduce their fear of the operation, lighten the inconvenience of post operation, and shorten the treatment period. In particular, it is worth considering that the use of laser-assisted therapies is associated with a marked reduction in the use of analgesics and anti-inflammatory medications compared with conventional procedures. This article is to state advantages and differences of Dental Laser treatment compared with Conventional procedures, and to emphasize to become well-acquainted with the precautions for safety and effective Dental Laser treatment. In case of operating Dental Laser with lack of the instructions, it will cause the unpredicted fatal results; therefore this treatment requires special care in its operation. Henceforward, it is anticipated that infinite treatment protocols will be introduced by applying Dental Laser, and this is to address the utilization of Dental Laser.

Effects of Acupuncture, Electro-acupuncture, Low Level He-Ne Laser Therapy at Oe-gwan($TE_5$) ${\cdot}$ Chogimup ($GB_{41}$) on L5 Spinal Nerve Ligation Model in Rats (건측(健測) 취혈(取穴) 다종(多種) 침자법(鍼刺法)이 백서(白鼠)의 신경병리성(神經病理性) 동통(疼痛)에 미치는 영향)

  • Jung, Jung-Hee;Cho, Myung-Rae;Wei, Tung-Sheun;Ryu, Chung-Ryeol
    • Journal of Acupuncture Research
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    • v.24 no.5
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    • pp.137-150
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    • 2007
  • Objectives : To find effects of acupuncture, electro-acupuncture, low level He-Ne laser therapy(LLLT) at $TE_5$, $GB_{41}$ in the neuropathic pain. We made experiment on rats ligated L5 spinal nerve like general herniation of nucleus pulposus(HNP). Methods : A model of neuropathic pain was made by isolating Left 5th lumbar spinal nerve of rats. Three days after the neuropathic surgery, acupuncture and LLLT, electro-acupuncture was injected at $TE_5$, $GB_{41}$ one time a day for a week. Each group was divided two. one is opposite side performed the surgery which is right, another is left side performed the surgery. After that, the author examined the withdrawal response of neuropathic rats' legs by van Frey filament and acetone stimulation. And also the author examined c-Fos, Nociceptin and KOR-3 in the midbrain central gray of neuropathic rats. Results : As we have observed the effect of mechanical allodynia, LT-R group were diminished on 6th day compared with control group, EA-L group, EA-R group and LT-L group were diminished on 7th day compared with control group. As we have observed the effect of cold allodynia, EA-R group were diminished on 6th day, 7th day compared with control group. As we have observed the effect of activity of c-Fos in the central gray part, EA-R group and LT-R group were diminished compared with control group. As we have observed the effect of activity of Nociceptin in the central gray part, EA-R group were a little increased compared with control group but it is not reliability. As we have observed the effect of activity of KOR-3 in the central gray part, EA-R group were significantly increased compared with control group. Conclusions : We have noticed that effect of acupuncture at opposite side of sickness and powerful stimulation could be more effective, because of EA-R group have more controllable effect all test we have done on the other hand EA-L group have only effect on mechanical allodynia. This study can be used in clinical therapy for neuropathic pain. But it is not reliability that Nociceptin have effectively to control pain. Therefore We have to follow up about that.

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Study on low-level laser therapy device according to the obesity development (비만치료기 개발에 따른 저준위레이저에 관한 연구)

  • Lee, Sang-sik;Kim, Jun-tae;Jeong, Jin-hyoung;Kim, Nam-Sun
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.9 no.1
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    • pp.82-89
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    • 2016
  • And by entering into an aging society with economic growth "beautiful and healthy desire to live', aesthetics industry as promote interest in 'Anti-aging' is emerging as a promising business increased significantly the skin care market. However, the management of the hospital or the temporal order to receive professional care providers, spatial, and cost constraints caused many companies to solve this problem began to approach the Home Care Area. Global trends in personal skin care market has been activated, the domestic has been activated at low cost, private market due to the recession. We have performed this test in order to develop a skin care device for home in order to compensate for this point.In this paper, we develop a low-level laser to create a personal skin care products and sought to incorporate them into the skin cosmetic.Expand the pores by using the low-level laser to the skin by to the dermal layer of the skin was penetrated aim experiment the ampoule, and by a comparison of the medical low-level laser reliability and determine the effectiveness or absence of the performance and efforts to commercialize.

Effects of Low Level Laser Therapy on Oral Mucositis Caused by Anticancer Chemotherapy in Pediatric Patients (소아 암 환자에서 항암제 치료 후 발생한 구내염에 대한 저출력 레이저의 효과)

  • Kim, Hae-Ja;Rho, Shi-Youn;Shin, Yong-Sup
    • The Korean Journal of Pain
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    • v.14 no.1
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    • pp.51-55
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    • 2001
  • Background: Oral mucositis is a common complication of anticancer chemotherapy. The sequelae of this consist of an increased risk of infection, moderate to severe pain, compromised oral function, and bleeding. This study was performed to evaluated the effects of the He-Ne laser and the Ga-Al-As laser on oral mucositis caused by anticancer chemotherapy in pediatric patients. Methods: There were 3 cases of osteosarcoma and 6 cases of leukemia. All patients received He-Ne laser (632.8 nm wavelength, power 60 mW) application on 400-600 Hz scanning for 5-20 minutes and Ga-Al-As laser (904 nm wavelength, power 40 mW) application by fiberoptic hand piece placed in immediate proximity to the tissue without direct contact with it for 30 seconds per point for 5 days per week. During the application patients wore wavelength-specific dark glasses and were instructed to keep their eyes closed. Results: The mean number of treatments with oral intake was $4.89{\pm}0.64$. The mean number of total treatments was $9.44{\pm}2.59$. There were no significant side effects during and after the laser treatments. Conclusions: He-Ne laser and Ga-Al-As (IR) laser treatment were well tolerated and reduced the severity and duration of chemotherapy-induced oral mucositis in pediatric oncologic patients.

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Analysis of Level Characteristics of 630nm Light Emitting Diode Module (630nm Light Emitting Diode 모듈의 레벨 특성 평가)

  • Kim, Tae-Gon;Cheon, Min-Woo;Park, Yong-Pil;Kim, Seong-Hwan;Song, Chang-Hun;Kim, Young-Su
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.347-348
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    • 2006
  • This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. The equipment have been manufactured by using the High Bright Light Emitting Diode and TLC5941 integrated circuit. As a result, 630nm Light Emitting Diode Module was made for the optimization of irradiation condition. And we confirmed the current change according to increase of the level of Light Emitting Diode Module.

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Effects of acupuncture and low level laser acupuncture therapy (LLLAT) at Yolgyol (LU7), Yogu (LR5) in hyperlipemia rats induced by high rat diet (고지혈증 유발 백서에서 열결 ${\cdot}$ 여구에 대한 침자 및 강도별 레이저조사가 체중, 식이효율, 지질대사 및 동맥경화지수에 미치는 영향)

  • Na, Chang-Su;Youn, Dae-Hwan;Cho, Myung-Rae;Kang, Ja-Don
    • Korean Journal of Acupuncture
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    • v.24 no.1
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    • pp.113-129
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    • 2007
  • Objectives : This research was performed to investigate the effect of acupuncture using invasive low level laser therapy (LLLT) at Yolgyol (LU7) + Yogu (LR5) on weight gain, food intake, food efficiency, lipid metabolism, atherogenic index, HTR (HDL-cholesterol to total cholesterol ratio) and morphological change of hepatic tissue in hyperlipdemia rats. Methods : Experimental groups were divided into high fat diet group (Control group), high fat diet and acupuncture therapy group at LU7 + LR5 (AT group), high fat diet and acupuncture group using 10 mW LLLT at LU7 +LR5 (LA10 group), high fat diet and acupuncture group using 20 mW LLLT at LU7+LR5(LA20 group), high fat diet and acupuncture group using 60 mW LLLT at LU7 + LR5 (LA60 group), once per 3 days during 9 weeks. Results : Body weight was decreased significantly in AT and LA20 groups compared with Control group. Food intake was increased significantly in LA60 group compared with Control group. Food efficiency was decreased significantly in LA10, LA20 and LA60 groups compared with control group. In the lipid metabolism, total cholesterol was decreased significantly in AT, LA10, LA20 and LA60 groups, triglyceride was decreased significantly in LA10, LA20 and LA60 groups, TG/HDL-cholesterol ratio was decreased significantly in LA 60 group compared with control group. In the morphological change, hepatic tissue were not showed balloning degeneration and irregular arrangement of hepatic cell in LA10 and LA20 groups with control group. Conclusions : Acupuncture using LLLT at LU7+LR5 can manage hyperlipemia by controlling body weight, food intake, food efficiency ratio and lipid metabolism.

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