Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Influence of Diode Laser (808 nm) on a Rat Anterior Cruciate Ligament Transection Model of Osteoarthritis

전십자인대 단열을 통한 랫드 골관절염모델에서 다이오드레이져 (808 nm)의 영향

  • Park, Seongkyu (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Minar, Maruf (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Hwang, Yawon (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Somin (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Minhyeok (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Seok-Hwa (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Gonhyung (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
  • 박성규 (충북대학교 수의과대학 수의외과학 교실) ;
  • ;
  • 황야원 (충북대학교 수의과대학 수의외과학 교실) ;
  • 김소민 (충북대학교 수의과대학 수의외과학 교실) ;
  • 박민혁 (충북대학교 수의과대학 수의외과학 교실) ;
  • 최석화 (충북대학교 수의과대학 수의외과학 교실) ;
  • 김근형 (충북대학교 수의과대학 수의외과학 교실)
  • Accepted : 2013.09.26
  • Published : 2013.10.31
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Abstract

The study was aimed to investigate the influence of diode laser on osteoarthritis (OA) of stifle joint induced by anterior cruciate ligament transection (ACLT). Sixty 10-week-old male Sprague-Dawley rats were used in this study. Right stifle joint was operated to create ACLT or sham. There were five study groups: control, Sham, ACLT, ACLT + Laser irradiation (ACLT+L) and ACLT + meloxicam administration (ACLT+M). Low-level laser therapy (LLLT) was applied at the operated stifle joint twice a week using an 808-nm indium-gallium-arsenide (InGaAs) diode laser during 8-week experimental period. Radiographical, gross morphological and histopathological findings were examined at 2, 4 and 8 weeks post-surgery. Radiography, CBC and chemistry tests showed no significant difference between groups. ACLT+L group showed remarkable cartilage damages compared with sham group morphologically and histopathologically at 2, 4 and 8 weeks after surgery. ACLT+M group also had more cartilage damages compared with sham group. Low-level laser therapy (LLLT) showed limitation to prevent progression of OA in the rat anterior cruciate ligament transection models; on the contrary it accelerated cartilage damage. It is assumed that the aggravating results of LLLT in this study might be due to excessive unstable movement of stifle joint from the pain-relieving effect of LLLT, rather than direct damaging effect of irradiation since LLLT did not affect cell viability.

본 연구는 다이오드레이저가 골관절염에 주는 영향을 평가하기 위해 실시되었다. 연구에는 10주령의 수컷 Sprague-Dawley 랫드 60마리를 사용하였고 전십자인대의 절단 (ACLT)으로 골관절염을 유발하였다. 대조군, sham군, ACLT군, ACLT와 레이저조사를 병행한 군 (ACLT+L), ACLT와 meloxicam 투약을 병행한 군 (ACLT+M)의 다섯 개의 실험군으로 나누어 연구를 진행하였다. 8주간의 연구 기간 동안 808-nm indium-gallium-arsenide (InGaAs) 다이오드레이저 조사 방법으로 Low-level laser therapy (LLLT)를 일주일에 두 번 랫드의 수술 한 오른쪽 다리에 조사하였다. 수술 후 2주, 4주, 8주에 방사선학적, 형태학적, 조직병리학적 평가를 실시하였다. 8주에 실시한 방사선학적 평가와 일반 혈액검사, 혈청 화학 검사에서는 군 간의 유의한 차이가 나타나지 않았다. 형택학적 방법과 조직병리학적 방법으로 수술 후 2주, 4주, 8주의 ACLT+L 군과 sham 군을 비교 했을 때 ACLT+L 군에서 유의하게 연골의 손상정도가 높은 것으로 나타났다. ACLT+M 군 또한 sham 군과 비교했을 때 연골의 손상이 심한 것으로 나타났다. LLLT는 ACLT 모델에서 골관절염을 예방하는 효과는 제한적이었으며, 오히려 연골의 손상을 가속화 시키는 것으로 평가 되었다. LLLT가 세포의 생존에는 영향을 주지 않으므로 레이져에 의한 직접적인 연골손상 발생보다는 레이저의 통증완화효과로 인해 불안정한 무릎관절을 계속해서 사용함으로써 연골의 손상이 가속화 된 것으로 생각 된다.

Keywords

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