• 대한한의학방제학회지
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• 제24권3호
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• pp.153-161
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• 2016

Chronic alcoholic myopathy is one of the most common skeletal muscle disorders. It is characterized by a reduction in the entire skeletal musculature, skeletal muscle weakness, and difficulties in gait. Patients with alcoholic hepatitis and cirrhosis have severe muscle loss that contributes to worsening outcome. Although the myopathy selectively affects Type II (fast twitch, glycolytic, anaerobic) skeletal muscle fibers, total skeletal musculature is reduced. The severity of the muscle atrophy is proportional to the duration and amount of alcohol consumed and leads to decreased muscle strength. The mechanisms for the myopathy are generally unknown but it is not due to overt nutritional deficiency, nor due to either neuropathy or severe liver disease. Skeletal muscle mass and protein content are maintained by a balance between protein synthesis and breakdown and in vivo animal models studies have shown that ethanol inhibits skeletal muscle protein synthesis. Daekumeumja is a traditional Korean medicine that is widely employed to treat various alcohol-induced diseases. Muscle diseases are often related to liver diseases and conditions. The main objective of this study was to assess that Daekumeumja extract could have protective effect against alcoholic myopathy in a Sprague-Dawley rat model. Rats were orally given 25% ethanol (5ml/kg, body weight) for 8 weeks. After 30 minutes, rats were administrated with Daekumeumja extract. Controls were similarly administrated with the vehicle alone. The weights of gastrocnemius, soleus and plantaris muscles were assessed and the morphologic changes of gastrocnemius and plantaris muscles were also assessed by hematoxylin and eosin staining. In results, The muscles from ethanol treated rats displayed a significant reduction in muscle weight and average cross section area compared to Normal group. Daekumeumja extract treated group showed increased muscle weight and muscle fiber compared to the ethanol treated group. It was concluded that Daekumeumja extract showed ameliorating effects on chronic alcohol myopathy in skeletal muscle.

• 대한한의학방제학회지
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• 제28권2호
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• pp.169-177
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• 2020

Objectives : This experiment was designed to assess the single oral toxicity of Ethanol Extract Inulae Flos (IF) ethanol extracts. IF is one of the important herbs to remove phlegmy which is the viscous turbid pathological product that can accumulate in the body, causing a variety of diseases. Nevertheless, there has been a lack of research on the pharmacology toxicity of IF. Methods : In this study, IF was orally administered to 5 weeks ICR mice as an oral dose of 2,000 or 3,000 or 5,000 mg/kg. The condition of the mice was observed for 14 days and their weights were measured every two days. Results : None of the mice died for 14 days. The abnormal clinical symptoms and anatomical signs of toxicity were not found in any treatment groups. The gain of net body weight was observed. There was also no significant difference in the organ weight. The serum biochemistry and hematological analysis showed a decrease in BUN, red blood cells, white blood cells and platelets although within the normal ranges. Conclusions : These results suggest that the 50% lethal dose of IF is more than 5,000 mg/kg. This could be thought that IF is a safe drug without acute toxicity and side effects. However, IF showed some weight loss and change in blood test, so it will need to be careful when using it for high doses.

• 동의생리병리학회지
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• 제19권2호
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• pp.530-535
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• 2005

A Korean traditional herbal formular, Taeumbiman-tang(TBT), was based on Taeumjowi-tang, currently the most widely used herbal formula compound, and which is known to be safe and have a positive effect on adult obesity. TBT was given to obese children for thirty days, and was found to be clinically safe and effective. The subjects were children who had been admitted into the hospital to be treated for obesity, that had more than 20% relative body weight. Originally there were 19 subjects, but 5 dropped out of the experiment. There were 5 girls and 9 boys, whose average ages were $11.00\;{\pm}\;2.60$ years, average weight was $54.01\;{\pm}\;18.59\;kg$. As a pretest, all of the volunteers were examined for height, weight, blood pressure, pulse rate, EKG, and chest radiography. The subjects underwent interview, body measurement tests, blood tests, and safety tests under 8 hour fasting state. After 15 days of TBT intake, the safety tests and the interview were conducted. The interview, body measurement tests, blood tests, and safety tests were conducted again after the 30 day trial was completed. The short term effects of TBT on obese children is weight loss. It did not cause any significant changes in the subject's livers, hearts, and kidneys, and clinically dangerous side effects or withdrawal symptoms were not observed.

• 대한한의학방제학회지
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• 제30권4호
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• pp.281-291
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• 2022

Objectives : In this study, acute oral toxicity test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis. Methods : Pomace Schisandra chinensis extracts were orally administered to SD-rats(female, n=3) without a control group according to the 'OECD guidelines'. After, mortality and clinical signs were observed, and the deceased animals were subjected to an autopsy. In addition, acute oral toxicity test was sequentially performed in step I (300 mg/kg), step II(300 mg/kg), step III(2,000 mg/kg), and step IV(2,000 mg/kg) according to the mortality. Results : There were no abnormalities caused by pomace Schisandra chinensis extracts in step I and step II. However, one animal each died in step III and step IV. In addition, clinical signs(salivation, decrease in food intake, prone position, decrease of locomotor activity, loss of locomotor activity, convulsion, hypothermia, lacrimation, staining around mouth, soiled perineal region, reddish urine, chromaturia, decrease of fecal volume, lying on side, blackish stool, no stool, compound-colored stool, refusal to feed, excitement, hypersensitivity, rigidity, dorsal position, etc.) were observed. But, no clinical signs were observed from 5th day, and experiment animals recovered completely. Conclusions : As a result of this study, pomace Schisandra chinensis extracts may exhibit acute toxicity at concentrations of 2,000 to 5,000 mg/kg, and the GHS classification was designated as 'Category 5'.

• 대한한의학방제학회지
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• 제30권4호
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• pp.269-280
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• 2022

Objectives : In this study, it was investigated the effects of solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis extract (PS) and its action mechanism on dexamethasone-induced muscle atrophy in mice. Methods : In mice, muscle atrophy model was induced by dexamethasone (5 mg/kg, I.p) once daily for 2 weeks and with PS extract administration (100 and 300 mg/kg, p.o.) as treatment groups. The changes in body weights, grip strength, Treadmill test, muscle weights, and the expression of atrophy-related genes were measured in muscle atrophy mice. The histological changes of gastrocnemius tissues were also observed by H&E staining with measurement of myofiber size. Results : The administration of PS extract increased significantly body weights, grip strength, treadmill test and muscle weights in muscle atrophy mice. PS extract administration increased significantly the area of myofibers and inhibited structural damages of muscle and increased significantly the expression of myogenin and decreased significantly the expression of MuRF1, Atrogin1 and phosphorylation of AMPK and PGC1α in muscle tissues of muscle atrophy mice. Conclusions : These results indicate that PS extract has a improvement effects on muscle atrophy with stimulation of myogenic differentiation and inhibition of mRNA degradation that could be related with the activation of AMPK and PGC1α signaling pathways in muscle. This suggests that PS extract can apply to treat muscle atrophy in clinics.

• 대한한의학방제학회지
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• 제25권2호
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• pp.223-251
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• 2017

Objectives : A lot of experiment results of Yugmijihwangtang(YM) are reported in various kinds of journals. Many of them report on the new effects that are not recorded in the traditional medical texts. So it is necessary to take it into consideration that newly reported effects could be of help to clinical practice, because this process of comparison of Donguibogam and scientific experiment results will have basis to lead into the evidence based medicine. Methods : We compared the effects of in Donguibogam and the experiment results of YM. Results : The effects of YM in Donguibogam are to replenish essence and marrow, and to treat red wen, fatigue, treat hypouresis, urinary sediment, urinary urgency, hematuria, hydrocephalus, speech and movement retardation, yin-deficiency, diabetes mellitus, nonalcoholic fatty liver, melanoma, disability to see near and far sight, tinnitus, hearing loss, alopecia, angiogenesis, cough, cough at night, trachyphonia, and, infantile convulsion. The experiment results of YM since 2000 in both Korea and China are to inhibit atopic dermatitis, renal interstitial fibrosis, anti-oxidant, emphysema, stress, glomerulosclerosis, diabetic nephropathy, chronic glomerulonephritis, hemorrhage, plantar sweating, dermal aging, kidney aging, bone loss, breast cancer, pathological myocardial cell, primary liver cancer, thrombosis, osteoporosis, intrauterine growth retardation, chronic renal failure, IgA nepropathy, slow cerebral development, and hippocampal tissue lesions on the one hand, and to help bone formation, renin-angiotensin- aldosterone system, cerebral recovery, cognitive function and expression, osteoblast proliferation and differentiation, learning and memory, cold-tolerance and oxygen deficit-tolerance and anti-fatigue, endometrial formation, humoral and cell-mediated immunity, immune regulation effect, Hypothalamus-Pituitary-Ovary Axis, and spermatogenesis, on the other hand. Conclusion : When we compared the effects of YM with the experiment results of YM, there existed a considerable gap between them. So, from now on, it is expected that a great effort and consideration are needed to solve these gaps from an academic and clinical point of view.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 추계학술대회 논문집
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• pp.502-502
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• 2007

전차선로는 각 경간마다 양단으로 지지점을 갖게 되므로 지지점에서의 탄성도와 경간중앙에서의 탄성도가 같을 수 없다. 이 두 지점의 탄성도의 차이를 나타내는 계수로 불균일율이라는 지수가 정의되어 널리 사용되어왔다. 이 불균일율은 전차선 장력과 경간 길이에 크게 영향 받는다. 그러나 드로퍼의 배치 간격에 따라서도 달라진다. 이 불균일율은 전차선로의 동적인 특성을 예측하는 하나의 변수로 활용되고 결국 집전 성능을 상대 비교하여 판단하는데 활용되며, 직접적으로는 드로퍼 간격을 설계하는데 있어서 중요한 파라미터로 사용되고 있다. 그러나 이 불균일율은 전차선로의 동적 성능을 적절히 반영하는 지수가 되지 못하고 있다고 판단하고 있다. 불균일율의 정의인 경간 중앙에서의 탄성율과 경간 끝 지정의 탄성율과의 단순한 차이 값의 크기만으로는 드로퍼의 수와 배치 간격에 따른 특성을 반영하지 못한다고 판단된다. 이를 여러 가지 다양한 케이스를 가정하여 탄성도 해석 프로그램을 이용하여 평가해본다. 평가 결과를 토대로 드로퍼 수에 따른 보정계수를 갖는 새로운 불균일율 평가 지수를 제시하고자 하며 이 새로운 지수가 기존의 지수보다 보다 집전 성능을 판단하는데 유용한 데이터가 됨을 증명한다. 또한 아울러 불균일율이 다른 가선 구조에 대하여 팬터그래프의 1단과 2단의 스프링 계수의 크기와의 조합과 상환 관계에 따른 집전 성능의 차이를 전차선로-팬터그래프 동역학 시뮬레이션 프로그램을 사용하여 평가한다. 이를 통하여 불균일율에 따른 보다 적절한 팬터그래프 동역학 모델을 제시한다.

• 대한토목학회논문집
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• 제28권6B호
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• pp.635-642
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• 2008

개수로 난류흐름내 잠기지 않는 저항체들이 에너지 손실에 미치는 영향을 수리실험을 통해 연구하였다. 개수로의 잠기지 않는 구조물이나 식생을 연구하기 위하여 정방형 다열기둥을 실험에 사용하였다. 실험수로에 흐름방향 다열기둥을 횡방향으로 1열 및 2열로 배치하고 평균적 등류수위를 유지하여 일련의 유량과 수로경사를 조건으로 실험을 수행하고 측정하였다. 실험결과를 Manning공식에 적용하여 바닥마찰과 기둥의 형상저항에 의한 흐름저항 및 에너지손실을 표현하는 상당저항계수 값을 등류수심과 기둥의 이격거리를 변화시키며 산정하였다. 그리고 실험을 통해 산정된 값과 항력상호작용계수를 포함하는 운동량방정식으로부터 유도된 상당저항계수의 이론식과 비교하였다. 이론식과 실험치의 비교로부터 기둥에 의한 흐름저항의 상호작용이 수로전체의 에너지손실에 미치는 영향이 매우 중요함을 알 수 있었다. 그리고 기둥폭에 대한 기둥이격거리가 약 2.2배 이하 일 때 값이 감소하며, 항력상호작용계수가 기둥이격거리에 지배적으로 의존한다는 사실로 부터 이론식에 나타난 값이 등류수심에 2/3승으로 증가함이 유효함을 알 수 있었다.

• 한국해안·해양공학회논문집
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• 제26권6호
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• pp.343-351
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• 2014

유공벽에 의한 파의 반사율과 투과율은 주로 유공벽의 공극률과 벽두께 그리고 입사파의 주기 및 비선형성에 의해 결정된다. 이중에서 벽두께는 유공벽의 수두손실계수와 관성저항길이에 영향을 미치므로 매우 중요하다. 그러나 기존의 연구에서는 예연 오리피스의 수두손실계수를 사용함으로써 수두손실계수에 미치는 벽두께 효과를 무시 또는 부정확하게 고려하였거나, 벽두께가 수두손실계수에 미치는 영향은 고려하였더라도 관성저항 길이를 무시함으로써 벽두께가 유공벽의 반사율과 투과율에 미치는 영향이 적절히 고려되지 못 하였다. 따라서 본 연구에서는 다양한 두께의 유공벽을 제작하여 파의 반사율 및 투과율 측정 수리실험을 수행하였으며, 그 결과를 기존의 해석해에 의한 값과 비교하였다. 비교결과 기존의 해석해 들이 벽두께의 효과를 적절히 반영하지 못하고 있음을 확인하였으며, 벽두께 효과를 정도 높게 고려할 수 있는 새로운 수두손실계수 산정식이 필요함을 확인하였다.

• 대한한의학방제학회지
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• 제22권2호
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• pp.105-120
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• 2014

Objectives : This study was investigated the improvement effects of Gangjihwan (DF) and combination of Gangjihwan and Gamisochehwan (GSH) on obesity in a high fat diet-fed obese mouse model. Methods : Eight-week-old C57BL/6N mice were divided into four groups: a normal lean group given a standard diet, an obese control group given a high fat diet, and DF and DF+GSH groups given a high fat diet with DF (40 mg/kg), and DF+GSH (80 mg/kg), respectively. After 8 weeks of treatment, body weight gain, feeding efficiency ratio, blood lipid markers, fat weight and histology were examined. Results : 1. Body weight gain and fat mass were significantly decreased in DF and DF+GSH groups compared with control. The extent of decreases was eminent in DF+GSH group. 2. Feeding efficiency ratio and circulating concentration of leptin were decreased in DF and DF+GSH groups compared with control. These decreases were significant in DF+GSH group. 3. Consistent with their effects on body weight gain and fat mass, circulating concentrations of triglyceride, glucose and insulin were decreased in DF and DF+GSH groups compared with control. 4. The size of adipocytes was decreased by DF and DF+GSH compared with control, whereas the adipocyte number per unit area was increased by them, suggesting that DF and DF+GSH decreased the number of large adipocytes. 5. Consistent with their effects on body weight gain, liver fibrosis was also improved in DF and DF+GSH groups compared with control. Conclusions : In conclusion, these results suggest that DF and DF+GSH groups decrease feeding efficiency ratio, plasma leptin concentration, blood anti-obesity biomarkers and fat mass, improves body weight gain contributing to the inhibition of liver fibrosis. In addition, these effects were more effective in DF+GSH combination group than in DF-only group.