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http://dx.doi.org/10.14374/HFS.2022.30.4.281

Evaluation of Acute Toxicity of Pomace Schisandra chinensis Extracts Using SD-rats  

Seokho, Kim (QBM Co. Ltd.)
Bo Ra, Yoo (Croen Inc.)
Young-Suk, Kim (Glucan Co. Ltd.)
Jong-Min, Lim (Glucan Co. Ltd.)
Bon-Hwa, Ku (Glucan Co. Ltd.)
Kyeong Tae, Kwak (QBM Co. Ltd.)
Byeong Yeob, Jeon (QBM Co. Ltd.)
Publication Information
Herbal Formula Science / v.30, no.4, 2022 , pp. 281-291 More about this Journal
Abstract
Objectives : In this study, acute oral toxicity test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis. Methods : Pomace Schisandra chinensis extracts were orally administered to SD-rats(female, n=3) without a control group according to the 'OECD guidelines'. After, mortality and clinical signs were observed, and the deceased animals were subjected to an autopsy. In addition, acute oral toxicity test was sequentially performed in step I (300 mg/kg), step II(300 mg/kg), step III(2,000 mg/kg), and step IV(2,000 mg/kg) according to the mortality. Results : There were no abnormalities caused by pomace Schisandra chinensis extracts in step I and step II. However, one animal each died in step III and step IV. In addition, clinical signs(salivation, decrease in food intake, prone position, decrease of locomotor activity, loss of locomotor activity, convulsion, hypothermia, lacrimation, staining around mouth, soiled perineal region, reddish urine, chromaturia, decrease of fecal volume, lying on side, blackish stool, no stool, compound-colored stool, refusal to feed, excitement, hypersensitivity, rigidity, dorsal position, etc.) were observed. But, no clinical signs were observed from 5th day, and experiment animals recovered completely. Conclusions : As a result of this study, pomace Schisandra chinensis extracts may exhibit acute toxicity at concentrations of 2,000 to 5,000 mg/kg, and the GHS classification was designated as 'Category 5'.
Keywords
Pomace Schisandra chinensis; up-cycling; acute toxicity; OECD; GHS;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Szopa A, Ekiert R, Ekiert H. Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies. Phytochem Rev. 2017;16(2):195-218.   DOI
2 Kopustinskiene DM, Bernatoniene J. Antioxidant Effects of Schisandra chinensis Fruits and Their Active Constituents. Antioxidants (Basel). 2021; 10(4):620.
3 Hu G, Qi Z, Wang A, Jia J. Effects of Deacidification on Composition of Schisandra chinensis Ethanolic Extract and Studies on Acute Toxicity in Mice. Molecules. 2020;25(24):6038.   DOI
4 Nowak A, Zaklos-Szyda M, Blasiak J, Nowak A, Zhang Z, Zhang B. Potential of Schisandra chinensis (Turcz.) Baill. in Human Health and Nutrition: A Review of Current Knowledge and Therapeutic Perspectives. Nutrients. 2019;11(2):333.
5 Jiang Y, Fan X, Wang Y, et al. Hepato-protective effects of six schisandra lignans on acetaminophen-induced liver injury are partially associated with the inhibition of CYP-mediated bioactivation. Chem Biol Interact. 2015;231:83-89.   DOI
6 Hu D, Yang Z, Yao X, et al. Dibenzocyclooctadiene lignans from Schisandra chinensis and their inhibitory activity on NO production in lipopolysaccharide-activated microglia cells. Phytochemistry. 2014;104:72-78.   DOI
7 Jeong EJ, Lee HK, Lee KY, et al. The effects of lignan-riched extract of Shisandra chinensis on amyloid-β-induced cognitive impairment and neurotoxicity in the cortex and hippocampus of mouse. J Ethnopharmacol. 2013;146(1):347-354.   DOI
8 Park HJ, Cho JY, Kim MK, et al. Anti-obesity effect of Schisandra chinensis in 3T3-L1 cells and high fat diet-induced obese rats. Food Chem. 2012;134:227-234.   DOI
9 Kim J.W., Ku S.K., Han M.H., Kim K.Y., Kim S.G., Kim G.Y., Hwang H.J., Kim B.W., Kim C.M., Choi Y.H. The administration of Fructus Schisandrae attenuates dexamethasone-induced muscle atrophy in mice. Int. J. Mol. Med. 2015;36:29-42.   DOI
10 Ekiert R.J., Szopa A., Ekiert H., Krzek J., Dzik E. Analysis of lignans in Schisandra chinensis fruits, leaves, biomasses from in vitro cultures and food supplements. J. Funct. Foods. 2013;5:1576-1581.   DOI
11 Mocan A., Crisan G., Vlase L., Crissan O., Vodnar D.C., Raita O., Gheldiu A.M., Toiu A., Oprean R., Tilea I. Comparative studies on polyphenolic composition, antioxidant and antimicrobial activities of Schisandra chinensis leaves and fruits. Molecules. 2014;19:15162-15179.   DOI
12 Szopa A., Ekiert H. The importance of applied light quality on the production of lignans and phenolic acids in Schisandra chinensis (Turcz.) Baill. cultures in vitro. Plant Cell Tissue Organ Cult. 2016;127:115-121.   DOI
13 Korea Food and Drug Administration. Notification No. 2009-116 Testing Guidelines for Safety Evaluation of Drugs. (2009) Aug 24;
14 OECD_423. Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. OECD; 2002.
15 Panossian A, Wikman G. Pharmacology of Schisandra chinensis Bail.: an overview of Russian research and uses in medicine. J Ethnopharmacol. 2008;118(2):183-212.   DOI
16 Choo SH, Sung HH, Chae MR, et al. Effects of Schisandra chinensis extract on the relaxation of isolated human prostate tissue and smooth muscle cell. J Ethnopharmacol. 2014;156:271-276.   DOI
17 Lu Y, Chen DF. Analysis of Schisandra chinensis and Schisandra sphenanthera. J Chromatogr A. 2009;1216(11):1980-1990.   DOI
18 Kim, H.-J., Seo, Y.-M., Lee, E.-J., Chung, C., Sung, H.-J., Sohn, H.-Y., Kim, J.-S. Anti-proliferative and Pro-apoptotic Activities by Pomace of Schisandra chinensis (Turcz.) Baill. and Schizandrin. Journal of Life Science. 2018;28(4), 415-420.   DOI
19 Park WS, Koo KA, Bae JY, et al. Dibenzocyclooctadiene Lignans in Plant Parts and Fermented Beverages of Schisandra chinensis. Plants (Basel). 2021;10(2):361.
20 Li Z, He X, Liu F, Wang J, Feng J. A review of polysaccharides from Schisandra chinensis and Schisandra sphenanthera: Properties, functions and applications. Carbohydr Polym. 2018;184:178-190.   DOI
21 Park, B. N., & Lee, J.-W. Antioxidation activity of residue after omija (Schisandra chinensis) juice extract. Journal of Applied Biological Chemistry. 2017; 60(2) 95-100.   DOI
22 Roll R., Hofer-Bosse Th. And Kayser D. New Perspectives in Acute Toxicity Testing of Chemicals. Toxicol. Lett., Suppl. 1986;31-86.
23 Diener W, Siccha L, Mischke U, Kayser D, Schlede E. The biometric evaluation of the acute-toxic-class method (oral). Arch Toxicol. 1994;68(10): 599-610.   DOI
24 Diener W, Mischke U, Schlede E, Kayser D. The biometrical evaluation of the OECD modified version of the acute toxic class method (oral). Arch Toxicol. 1995;69(10):729-734.
25 Diener W, Schlede E. Acute Toxic Class Methods: Alternatives to LD/LC50 Tests. ALTEX. 1999;16(3):129-134.