• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.28 no.6
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• pp.434-439
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• 2002

Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. OSCC generally has a poor prognosis due to its tendency towards a local invasion and subsequent metastasis, which is mediated by multiple proteolytic enzymes and angiogenesis. Soy products contain high levels of isoflavonoids, including the tyrosine kinase inhibitor, genistein, which has been identified as a potent inhibitor of cell proliferation and in vitro angiogenesis. The purpose of this in vitro study is to evaluate the anti-cancer effect of genistein with respect to the angiogenesis and basement membrane invasion in OSCC. The highly invasive OSCC cell line, HSC-3 cells were cultured in the presence of $10{\mu}M$ genistein for 24h. To evaluate the effects of genistein on the invasiveness and the gelatinolytic activity, in vitro invasion assay and zymography were performed. In order to evaluate the effect on the VEGF and bFGF mRNA expression, RT-PCR and northern hybridization reaction, and chemiluminescence detection were applied. The in vitro invasion assay showed that the genistein treatment reduced the cellular invasion through the artificial basement membrane and significant difference between the control group and the genistein treated group was shown in MMP-2 activity. Especially, the 62 kDa activated form of MMP-2 in the control group was 1.8 times higher than that in the genistein treated group. The results of the northern blot analyses indicated that VEGF mRNA expression in the genistein treated group was significantly down regulated. This study showed that genistein inhibits angiogenesis and reduces basement membrane invasion in OSCC. It seems to support the possibility of genistein as an anti-cancer agent.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.31 no.3
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• pp.239-247
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• 2005

This study demonstrated that xenogenic human marrow mesenchymal stem cells (hMSCs) could elicit the regeneration of the sensory nerve after axotomy in the adult rats infraorbital nerves without immunosuppression. For this, we evaluated the behavioral testing for functional recovery of the nerve and histological findings at weeks 3 and 5 compared to controls. Xenogenic hMSCs did not evoke any significant inflammatory or immunologic reaction after systemic and local administrations. HMSCs-treated rats exhibited significant improvement on sensory recovery tested with von Frey monofilaments. At 5 postoperative weeks, in the hMSCs treated nerve, expression of myelin basic protein (MBP), neurofilament (NF) at the site of axotomy was higher than control. And mRNA expression of neurotropin receptor Trk precursor (TrkPre), nerve growth factor receptor (NGFR) and neuropeptide (NPY) in trigeminal ganglion were also higher. The number of myelinated nerve at distal stump and cells in trigeminal ganglion were higher in hMSC treated rats. So it was supposed that transplanted MSCs contributed to reducing post-traumatic degeneration and production of neurotrophic factors. Immunofluorescence labeling showed small portion of hMSCs (<10%) expressed a phenotypic marker of Schwann cell (S-100). Xenogenic or allogenic mesenchymal stem cells might have immune privileged characteristics and useful tool for cell based nerve repair.

• Journal of Korean Medicine Rehabilitation
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• v.25 no.4
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• pp.175-182
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• 2015

The purpose of this study was to report an anaphylaxis after treated with Bee-venom acupuncture. Anaphylaxis is a clinical syndrome characterized by the acute system reaction of multiple organ systems to an IgE-mediated immunologic mediator release in previously sensitized individuals. We investigated the patients who had injected with Bee-venom in our clinic from March 2, 2014 to May 30, 2015. and two patients of anaphylaxis treated by Bee-Venom acupuncture were observed. One case of anaphylaxis was expressed clinically hypotension drowsy mentality, dyspnea, vomiting and so on. The other case was expressed itching sensation, urticaria, breathlessness, abdominal pain and so on. Based on this case, Bee venom-induced anaphylaxis can occur although preceding reactions are local or mild systemic ones. So, Korean medical doctor using Bee-Venom acupuncture must be prepare the system consider a countermeasure by anaphylaxis.

• Journal of the Korean Ceramic Society
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• v.46 no.5
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• pp.456-461
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• 2009

Chemical composition and status of chemical bonding of the YAG($Y_3Al_5O_{12}$) ceramics after the exposure to fluorine plasma have been investigated using X-ray photoelectron spectroscopy, with the analysis on its erosion behavior. On the surface, F showed the maximum content, decreasing with depth, meanwhile the cation composition remained almost constant, irrespective of the position. The peaks due to Y in the reaction layer consisted of two kinds, showing the Y-O and Y-F bonds. These surface modifications under fluorine plasma seem to promote the erosion of the YAG ceramics. Excess addition of $Al_2O_3$ or $Y_2O_3$ into stoichiometric YAG produced 2nd phases of $Al_2O_3$ and $YAlO_3$, respectively, resulting in the slight difference in the local erosion rates. But, the overall average erosion rate was not sensitive to such excess additions of $Al_2O_3$ or $Y_2O_3$.

• Tuberculosis and Respiratory Diseases
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• v.45 no.1
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• pp.191-196
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• 1998

Follicular bronchitis/bronchiolitis is pathologically characterized by peribronchiolar lymphoid follicles, which is one of reactive pulmonary lymphoid disorders. It is associated with 1) the result of infections such as mycoplasma, chlamydia etc., 2) immunodeficiency syndromes, 3) connective tissue diseases such as rheumatoid arthritis and Sjogren's syndrome and 4) local or systemic hypersensitivity reaction. And it can be also developed without obvious causes and associated diseases(idiopathic). Radiologically it represents as bilateral interstitial patterns of pulmonary infiltrates. In this case, a 49 year-old woman was presented with intermittent cough and sputum. On chest X-ray and CT, $5\times4$ cm sized mass in right upper lobe and paratracheal lymphadenopathies were detected, by which lung malignancy was suspected. Bronchoscopy, trans bronchial lung biopsy and transthoracic needle aspiration showed non-specific findings only. After right upper lobectomy, we could confirm a case of follicular bronchiolitis which presented as an unusual mass-like radiologic finding.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.805-810
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• 2007

Molecular recognition for a specific cation depending on the change of the oxidation state of the metal catalyst component contained in the hydrogel network has been studied in a self-oscillating hydrogel. The selfoscillating hydrogels are synthesized by the copolymerization of N-isopropylacrylamide (NIPAAm), lead methacrylic acid (Pb(MAA)2), and Ru(bpy)3 2+ monomer as a metal catalyst component. The recognition for a specific cation (in this study, Ca2+ has been used) is characterized by the adsorbed amount of Ca2+ into the gel. The recognition of the gels for Ca2+ is higher at the temperature below the LCST, and also higher at the oxidized state than at reduced state of the metal catalyst component which corresponds to a more swollen state. Moreover, a propagating wave induced by a periodic change of the oxidation state with the diffusion phenomena in the oscillating hydrogel shows a possibility for temporal and site-specific molecular recognition due to the local swelling of the gel.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.11
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• pp.1741-1751
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• 2004

It is commonly requested that the steam generator tubes wall-thinned in excess of 40% should be plugged. However, the plugging criterion is known to be too conservative for some locations and types of defects and its application is limited to a single crack in spite of the fact that the occurrence of multiple through-wall cracks is more common in general. The objective of this research is to propose the optimum failure prediction models for two adjacent through-wall cracks in steam generator tubes. The conservatism of the present plugging criteria was reviewed using the existing failure prediction models for a single crack, and six new failure prediction models for multiple through-wall cracks have been introduced. Then, in order to determine the optimum ones among these new local or global failure prediction models, a series of plastic collapse tests and corresponding finite element analyses for two adjacent through-wall cracks in thin plate were carried out. Thereby, the reaction force model, plastic zone contact model and COD (Crack-Opening Displacement) base model were selected as the optimum ones for assessment of steam generator tubes with multiple through-wall cracks. The selected optimum failure prediction models, finally, were used to estimate the coalescence pressure of two adjacent through-wall cracks in steam generator tubes.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.2
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• pp.219-227
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• 2013

The purpose of this study was to quickly and effectively identify the Nosema disease of bumblebees in Gangwon Province in Korea. Bumblebees are crucial pollinators of various crops, and microsporidia are the critical pathogens of these hosts. When bumblebees are infected with Nosema bombi, their abdomens can become distended. Paralyzed and infected workers often become sluggish and die early. We have identified the morphology of the microsporidium by light and electron microscopy, and found it to have fairly small oval spores, as has been described previously in many other articles. For the specific and sensitive diagnosis of the microsporidian parasite N. bombi in bumblebees, we have developed an improved method of the polymerase chain reaction (PCR) for expeditious diagnosis. Two pairs of primers were tested on N. bombi and the related microsporidia Nosema apis and Nosema sp., both of which infect Bombus ignitus and Bombus hypocrita sapporoensis. Furthermore, we have verified and analyzed the 16SrRNA sequence data of N. bombi in bumblebees by using the Basic Local Alignment Search Tool (BLAST) server at the National Center for Biotechnology Information.

• Journal of the Korean Chemical Society
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• v.47 no.1
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• pp.26-30
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• 2003

The dinuclear $Ag_2[Phen]_2[S_2P(OPr)_2]_2$(phen=1,10-phenanthroline; Pr=propyl), was prepared by the reaction of bis(dipropyldithiophosphato) silver(I) complex with 1,10-phenanthroline ligand, and its structure was determined by X-ray crystallography. The two dipropyldithiophosphato ligands each bridge two silver atoms to form an eight-membered $Ag_2S_4P_2$ ring, while the 1,10-phenanthroline molecule coordinates to a silver atom to complete the local tetrahedral geometry for the metal ion. The Ag-S bond distances are 2.559(1) and 2.567(1)${\AA}$, and the Ag-N bond distances are 2.366(3) and 2.471(3)${\AA}$.

• Journal of Environmental Science International
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• v.4 no.1
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• pp.63-70
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• 1995

Continuous measurements of ground-level ozone (O3) were made in five minutes intervals in the rural area of Korea from July 1993 to June 1994. This site is located in Chongwon, near latitude 36.4$^{\circ}$N, longitude 127.6$^{\circ}$E. The results show that the one-year mean value was 17 ppb, and monthly mean ranged from 6 to 47 ppb. A pronounced maximum in summer and a minimum in winter were found, and these were related to anthropogenic emission and photochemical reaction. Diurnal variations of ozone minimum at 07:00 - 08:00. During the period when ozone concentration was very high (> 80 ppb), the stable winds were from N and UW; on the other hand, when ozone concentration was very low, air movement in the large scale was from the North Pacific Ocean. This suggests that in the rural area the long range transport of anthropogenic pollutants from distant sources can contribute to the larger contribution than the generation of ozone from local sources in the rural area.