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XENOTRANSPLANT OF HUMAN BONE MARROW STROMAL CELLS; EFFECT ON THE REGENERATION OF AXOTOMIZED INFRAORBITAL NERVE IN RATS  

Park, Eun-Jin (Department of Dental Anesthesiology, College of Dentistry, Seoul National University)
Kim, Eun-Seok (Department of Dentistry, College of Medicine, Chungnam National University)
Kim, Jin-Man (Department of Pathology, College of Medicine, Chungnam National University)
Kim, Hyun-Ok (Department of Diagnosis and Laboratory Medicine, College of Medicine, Yonsei University)
Yum, Kwang-Won (Department of Dental Anesthesiology, College of Dentistry, Seoul National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.31, no.3, 2005 , pp. 239-247 More about this Journal
Abstract
This study demonstrated that xenogenic human marrow mesenchymal stem cells (hMSCs) could elicit the regeneration of the sensory nerve after axotomy in the adult rats infraorbital nerves without immunosuppression. For this, we evaluated the behavioral testing for functional recovery of the nerve and histological findings at weeks 3 and 5 compared to controls. Xenogenic hMSCs did not evoke any significant inflammatory or immunologic reaction after systemic and local administrations. HMSCs-treated rats exhibited significant improvement on sensory recovery tested with von Frey monofilaments. At 5 postoperative weeks, in the hMSCs treated nerve, expression of myelin basic protein (MBP), neurofilament (NF) at the site of axotomy was higher than control. And mRNA expression of neurotropin receptor Trk precursor (TrkPre), nerve growth factor receptor (NGFR) and neuropeptide (NPY) in trigeminal ganglion were also higher. The number of myelinated nerve at distal stump and cells in trigeminal ganglion were higher in hMSC treated rats. So it was supposed that transplanted MSCs contributed to reducing post-traumatic degeneration and production of neurotrophic factors. Immunofluorescence labeling showed small portion of hMSCs (<10%) expressed a phenotypic marker of Schwann cell (S-100). Xenogenic or allogenic mesenchymal stem cells might have immune privileged characteristics and useful tool for cell based nerve repair.
Keywords
Mesenchymal stem cell; Nerve regeneration; Infraorbital nerve; Schwann cell;
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1 Xu XM, Zhang SX, Li H, Aebischer P, Bunge MB: Regrowth of axons into the distal spinal cord through a Schwann-cell-seeded minichannel implanted into hemisected adult rat spinal cord. Eur J Neurosci 1999;11:,1723-1740   DOI   ScienceOn
2 Dezawa M, Takahashi I, Esaki M, Takano M, Sawada H: Sciatic nerve regeneration in rats induced by transplantation of in vitro differentiated bone-marrow stromal cells. Eur J Neurosci 2001;14:1771-1776   DOI   ScienceOn
3 Deng W, Obrocka M, Fischer I, Prockop DJ: In vitro differentiation of human marrow stromal cells into early progenitors of neural cellsby conditions that increase intracellular cyclic AMP. Biochem Biophys Res Commun 2001;282:148-152   DOI   ScienceOn
4 Gregg JM: A surgical approach to the ophthalmicmaxillary nerve trunks in the rat. J Dent Res 1973;52:392-394   DOI   ScienceOn
5 Verze L, Paraninfo A, Viglietti-Panzica C, Panzica GC, Ramieri G:Expression of neuropeptides and growth-associated protein 43 (GAP-43) in cutaneous and mucosal nerve structures of the adult rat lower lip after mental nerve section. Ann Anat 2003;185:35-40   DOI   ScienceOn
6 Vos BP, Strassman AM, Maciewicz RJ: Behavioral evidence of trigeminal neuropathic pain following chronic constriction injury to the rat's infraorbital nerve. J Neurosci 1994;14:2708-2723
7 Bennett GJ, Xie YKA: Peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 1988;33:87-107   DOI   ScienceOn
8 Tyndall DA, Gregg JM, Hanker JS: Evaluation of peripheral nerve regeneration following crushing or transection injuries. J Oral Maxillofac Surg 1984;2:314-318
9 Matzinger P: An innate sense of danger. Semin Immunol 1998;10:399-415   DOI   PUBMED   ScienceOn
10 Dezawa M: Central and peripheral nerve regeneration by transplantation of Schwann cells and transdifferentiated bone marrow stromal cells. Anat Sci Int 2002;77:12-25   DOI   ScienceOn
11 Cuevas P, Carceller F, Dujovny M, Garcia-Gomez I, Cuevas B, Gonzalez-Corrochano R, Diaz-Gonzalez D, Reimers D: Peripheral nerve regeneration by bone marrow stromal cells. Neurol Res 2002;24:634-638   DOI   ScienceOn
12 Waite PME, Tracey DJ: Trigeminal sensory system. In: Paxinos G, editor. The rat nervous system, 2nd edn., San Diego, CA: Academic Press 1995, pp. 705-724
13 Woodbury D, Schwarz EJ, Prockop DJ, Black IB: Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 2000;61:364-370   DOI   ScienceOn
14 Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM, Low WC:Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats. Exp Neurol 2002;174:11-20   DOI   ScienceOn
15 Liechty KW, MacKenzie TC, Shaaban AF, Radu A, Moseley AM, Deans R, Marshak DR, Flake AW: Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nat Med 2000;11:1282-1286
16 Anderson CC, Matzinger P: Danger: the view from the bottom of the cliff. Semin Immunol 2000;12:231-238   DOI   ScienceOn
17 Black IB, Woodbury D: Adult rat and human bone marrow stromal stem cells differentiate into neurons. Blood Cells Mol Dis 2001;27:632-636   DOI   ScienceOn