• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.11-18
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• 2020

Bacteria of the genus Enterococcus are of importance in food fermentations as well as their use as probiotics in humans and livestock. However, they are also important nosocomial pathogens that cause infections. Some strains are resistant to multiple antibiotics and possess virulence factors. The role of Enterococcus species in disease has raised issues on their safety for use in foods or as probiotics. First, this review summarized the positive and negative traits of Enterococcus spp. to illustrate the controversial nature of this bacterial genus and discussed the current genomic approaches can eliminate pathogenic strains. Then, this review examined the current status of starter development for traditional food fermentations and the regulation on the approval of novel food microorganisms in Korea to point out problems in the regulation. Based on the conclusions from the studies on Enterococcus spp., we suggested the direction of safety assessment of novel food microorganisms in Korea.

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.67-73
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• 2017

This study was to investigate the perception about the popularization of temple food, known as health food and adult disease prevention food as an object of total 248 residents in Youngnam region. From the result of this research above, it may be summed up as follows. This study has found out that the advantages of temple food showed the statistical significant difference according to the gender, age, and size of family (p<0.01). The reason 'because of health food' (66.7%) had such high percentage. The necessity of popularization of temple food had big difference according to the gender and age (p<0.01). In addition, this study has shown the statistical significant difference in the cooking form for popularization by age, and future improvement for popularization by age and size of family (p<0.001). And, this study has found out that the 'traditional way as it is' (57.3%) was high in the cooking form, and 'use of various materials' (27.4%) in the improvement. Furthermore, the necessity of using meat for temple food had significant difference according to the gender and age, and 'need' (33.9%) showed rather higher tendency. The necessity of using osinchae, five forbidden pungent roots like garlic, green onions, Korean leeks, wild chives, and asafetida had significant difference according to the gender, age and size of family (p<0.05, P<0.001). The 'need' (61.3%) was considerably high. Based on the above results, general customers thought that the need for popularization of temple food and use of osinchae was very positive.

• Korean Journal of Veterinary Research
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• v.56 no.4
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• pp.249-254
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• 2016

As the meat of black goats has become popular as a healthy food, domestic goat meat-related industries are steadily growing. However, previous studies are scarce of informations about the zoonotic disease originated from the black goat in Korea. In this study, we investigated Korean black goat's infectious diseases representing bovine tuberculosis, brucellosis, and Q fever. One hundred and eighty samples were collected from a local slaughter house located in Jeollanam-do. Three typical zoonotic diseases were separately examined by carrying out enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Histopathological test was additionally performed in tuberculosis. In case of tuberculosis, results of the PCR and histopathological test were negative but the ELISA results were positive in eight samples. In case of brucellosis, one out of the total samples was shown to be positive in the ELISA and none in the PCR. In case of Q fever, there were forty one positive in the ELISA and twenty positive in the real-time PCR. Those results indicate that the Korean black goat could be a natural reservoir in the possible chain-infections among human, cows and goats. Thus, further study needs in order to improve productivity as well as to prevent the zoonosis spreading and circulation of other livestock with the black goat in this country.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.127-132
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• 1996

This study was carried out to obtain good livestock production in comparison with 3 different pasture mixture (Control : TI = Potomac orchardgrass + Bastion perennial ryegrass + Fawn tall fescue + Regal white clover, T2 = Potomac orchardgrass + Bastion perennial ryegrass + Roa tall fescue + Regal white clover, T3 = Potomac orchardgrass + Bastion perennial ryegrass + Roa tall fescue + Tahora white clover) by grazing experiment used 45 dairy calves (about lOOkg liveweight) during the period from April to October, 1994 at the Isidole farm, Cheju. Daily liveweight gains of calves grazed during the experiment period were 331${\pm}$29, 352${\pm}$4, 356${\pm}$18g in treatment 1, 2 and 3 respectively, but did not get statistically significant difference. On compared the results with the period investigated, the period which was highest in daily liveweight gain was early grazing season b m May to June while the period shown to be lowest in daily liveweight gain was between late July and late August. Hehage intake rate did not get significant difference among treatments with results shown as 67${\pm}$1.2, 62${\pm}$5.1, 60${\pm}$9.6% in treatment 1, 2 and 3 respectively. And efficiency of conversation into animal product was high in treatment 2. It is considered that good results were not obtained in this study because tall fescue and white clover, important species in this trial did not cover the pasture well with slow establishment in 1st year after seeding.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.169-190
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• 2006

Researches in livestock are currently actively progressing to improve economically important traits using DNA markers. In cattle, the candidate genes have been selected based on their known functions in the target QTL (quantitative trait locus) region in order to identify QTN (quantitative trait nucleotide) for improving productivities. In this review, molecular genetic studies for the meat related traits, one of the major determinant of market prices, have been fully described. Also recent emerging microarray technique for identifying candidate genes in cattle has been discussed. In case of microarray, cDNA microarrays have been replaced to oligoarrays in order to minimize the experimental errors in cattle. Since the first draft of bovine genome sequences was appeared in the public domain, more markers in relation to the quantitative traits will be discovered in a short period of time and genes affecting difficult-to-measure traits, such as disease resistance, can also be selected for marker assisted selection in near future.

• Journal of Animal Environmental Science
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• v.17 no.1
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• pp.23-32
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• 2011

The purpose of this study is development of the sprayer that can effectively control pathogens. Image processing was used to analyze the sprayer. Experimental paper in the form of $5{\times}7$ 10 m intervals total of 35 sheets were installed. Experiment used binarized image data obtained by sprayed pigment, to analysis spray volume and distance. The experimental results show that 60 m distance was available to the spray. And spray rate was high up to 30 m. It can be used in livestock farms are expected.

• Development and Reproduction
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• v.19 no.2
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• pp.79-84
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• 2015

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.582-585
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• 2009

Bovine viral diarrhea virus (BVDV) is an economically important worldwide disease in livestock industry. In this study, the occurrence of BVDV in Korean indigenous cattle was performed by RT-PCR using nasal swab. Twelve of 21 cattle were identified as BVDV positive and classified as persistently infected (PI). These animals showed the occurrence of diseases such as diarrhea and pneumonia. BVDV PI outbreaks were found mostly in PI calves. Sequencing and phylogenetic analysis based on the 5'-untranslated region (UTR) showed that our case belonged to BVDV-2a. These results suggested that the nasal swab sampling was available method for the detection of PI animals, underscoring the need for BVDV control strategies in Korean indigenous cattle.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.287-296
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• 2005

This experiments was designed to observe the effect of period and dose of ingestion of grapes on renal failure in dog. After the dogs had been mixed of general feed, raisins from U.S.A and fresh grapes from Chile selling in Korea. We observed the clinical sign, and analyzed the blood and urine using by biochemical test. The experiment was executed by the two following groups. The experiment group 1. derived renal failure by supplying the mixed general feed with fresh grapes and dry grapes 15 g per weight kg, 2 times a day (AM 09, PM 21), the experiment group 2. executed to derive renal failure by supplying the mixed general feed with fresh grapes and dry grapes 40 g per weight kg, 2 times a day. Extraction of blood for analysis was conducted one time a day and clinical test for renal failure was executed by means of a blood analysis, biochemical analysis, urine analysis, excretory urography (E.U) and E.R.D-$screen^{TM}$ urine test (Heska, USA). The results of group 1 were normal ranges (BUN 9.0~22.6 mg/dl, creatinine 0.8~1.2 mg/dl, Ca 9.7~12.3 mg/dl, Pi 2.9~4.6 mg/dl), renal failure was not observed. On the 3rd day in group 2, azotemia was arisen from the increasing BUN 83 mg/dl (7~25 mg/dl), creatinine 2.3 mg/dl (0.5~1.4 mg/dl), when executed urine was tested by E.R.D-$screen^{TM}$ test using in the early kidney disease diagnosis, microalbumine state was high positive, and it showed stale delay by using excretory urography (EU). This study demonstrated that acute renal failure by grapes and raisins dependent on food dose, and specific characters of individual.

• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.133-137
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• 2012

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.